Fructan HK Video Protocol with K-FRUCHK

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Megazyme’s Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.
Note that this is a modified version of AOAC method 999.03/AACC Method 32.32.01 (K-FRUC) in which the overall assay principle remains unchanged but detection is performed through the enzymatic hexokinase/glucose-6-phosphate dehydrogenase system as opposed to the reducing sugar PAHBAH determination. The K-FRUCHK procedure has not received official method status.
This video will talk you through the assay principle and procedure, and explain how to perform the necessary calculations.
Chapters for this video can be found below:
00:02 Theory of the Analytical Procedure
02:07 Kit Description
03:12 Preparation of Reagent Solutions/Suspensions
05:30 Samples Containing 0-12% Fructan
07:55 Samples Containing 12-100% Fructan
Analysis of Fructan Content
10:47 a. Hydrolysis of Sucrose & Low DP Maltosaccharides
12:39 b. Hydrolysis of Fructan
17:14 Calculation of Fructan Content
• Product page at the Megazyme webstore: www.megazyme.c...
• Download the K-FRUCHK data booklet: support.megazy...
• Access the Mega-Calc for K-FRUCHK: support.megazy...