Total Coliform Count (TCC)_A Complete Procedure (ISO 4832:2006)

[@udayanagaanurada1176]

thanks a lot.. so badly needed this to recover my micobiological testing knowladge...

[@MicroChemsExperiments]

Thank you

[@ilililililiillli668]

Absolutely helpful thank you so much!!

[@MicroChemsExperiments]

Glad it helped!

[@biannekaulf9221]

Cool. I really understand the video. Thanks for making this!

[@MicroChemsExperiments]

Thanks

[@Patolitte]

Informative and comprehensive

[@MicroChemsExperiments]

Thank you so much. Stay with us.

[@sangitasahoo7599]

Its really informative. Can you plz make a swab test video

[@MicroChemsExperiments]

Sure. We will make soon

[@brianmarv]

Is this a Most Probable Number (MPN) method or Membrane Filtration Method (MFM)? Can it work for turbid water?

[@fatemehhassantabar4842]

really useful, thanks

[@MicroChemsExperiments]

Thank you

[@malaiyarasujanakiraman7535]

thank you so much

[@MicroChemsExperiments]

You're welcome!

[@Rajput_ff001]

How is Peptone salt solution preparation done because in this you have told something else about the formula to make it and in the video of Ecoli you had told something else, please do tell🙁🙁

[@zarqaarshad411]

Kindly upload protozoa, helminths, campylobacter, staphylococcus video as soon as possible I need this for my research

[@MicroChemsExperiments]

Noted

[@tasyanurafifahsyifafitriya3144]

Can i ask something mam, why VRBL media do not autoclave? and what happen if VRBL media di autoclave? pls answer me mam

[@ibrahimmohamed6209]

VRBL media should not be autoclaved due to its specific preparation requirements and the potential negative impact on its selective properties and overall performance in microbiological assays.

[@niketadixit9721]

Mam please explain vibrio Cholera ,vibrio monocytogenes

[@mohammadhammad5844]

Pls make a video for: Salmonella Staphylococcus E.coli using tbx media Listeria spore forming bacteria(aerobic , an aerobic) Thank you very much

[@MicroChemsExperiments]

Okay. We will make

[@GolamSaroar-k7p]

just remarkable !

[@MicroChemsExperiments]

Thanks

[@yasminmazur3142]

thank you very much, can you tell me what the minimum dilution required for solids? I see that the liquids are X10, can you do that with solids too?

[@MicroChemsExperiments]

Yes. You can. ×10 dilution is the minimum

[@SirajulIslam-vp9gp]

Very nice. Thank you....

[@MicroChemsExperiments]

Thank you too!

[@saniketbhosle3390]

Is it ok if we count the colonies manually if there's no colony counting machine. The video is really helpful Thanks....

[@MicroChemsExperiments]

Yes. You can count colonies manually.

[@Jinia21744]

Pls make a video for Thermophillic acidophillic bacteria and Heat resistant mold identification by using k agar ,plate count agar and Pda media for fruit juice.

[@MicroChemsExperiments]

Ok. we will make

[@salonipatil8544]

Whatu have used is durham's tube for gas production ? is it peptone watet??

[@MicroChemsExperiments]

BGLB broth is used along with Durham tube

[@InnocentUtondo]

Can same method be used for alcoholic beverages?

[@hajarzakzouk7458]

thank you so much for the video .. may i ask a question ? .. why did you poure 4 ml medium after solidifying ?

[@MicroChemsExperiments]

This overlay of media is needed to facilitate the growth of bacterial cell on the surface of the first layer of the media

[@ramkrishansharma6717]

Sir please make a video of coliform confirmation test

[@farheenzahidsiddiquie9766]

there are white colored center dots on pink color colony, are these coliform and to be counted?

[@MicroChemsExperiments]

Yes. Those are also coliform

[@rajanpatel3228]

I have pepton not enzymatically digested casein. Will it work?

[@MicroChemsExperiments]

Yes

[@hamini1375]

do you have vrba for finger test???for fingers of food factory

[@md.shahinsarkar1625]

Thank you

[@MicroChemsExperiments]

You are welcome

[@nehatiwari2521]

In this calculation dilution factor is 10 but you prepared the dilution only once?

[@JitendraKkushawaha999]

Please make a video on total plate count..

[@MicroChemsExperiments]

Noted. We will work on it soon.

[@EelasMusic]

I have a one question from you... I collected sample from the sewage water,and followed with 3 times serial dilution with bglb broth and incubated for the identification of coliform bacteria.after the production of gas I isolate them into(0.1ml) macconkey agar and did indole test for them for the confirmation.in plates I've got 60 colonies,so the CFU /ml was calculated...6x10^5 CFU /ml ...so,I need to dilute them into three samples.like 10 CFU/ml,100 CFU/ml and 1000 CFU/ml...so can u give me an idea how to do that.... And my second question after diluted them,I need to those cultures to grow inside the nutrient broth for the DNA extraction,so,how can I inoculate them,I mean after getting dilution,we should do the culture again which I mention in 1st question like culture into the agar plates,if I take the one colony from the agar plate define 1 ml?I mean let say we culture the agar plate from the 100cfu/ml sample if I take one colony from them means that colony contain 100cfu?

[@MicroChemsExperiments]

Eelam Music Production, Its soo tough to explain here to solve your question. But I'm trying.... Situation-1: You got 60 CFU from 0.1 BGLB broth culture ****Ans to the first question: 1. To get 10CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 59ml normal saline. 2. To get 100CFU/ml sample, dilute 1ml BGLB culture (600CFU) into 5ml normal saline. 3. To get 1000CFU/ml sample, you need to use lower dilution in the same way as done for 1 & 2. ****Ans to the second queation: A single colony contains millions of CFU. First dilute 1 colony in 100ml normal saline then culture 0.1ml from the diluted colony on nutrient agar. Count the CFU on nutrient agar plate. Now Calculate and dilute the normal saline as much as you need to find your desired bacterial concentration, on the basis of how much CFU you got on NA plate. If you dont get the expected result by following my guideline then try the bellow procedure: First culture a coliform bacteria in nitrient broth, centrifuge at 4000rpm for 5 min. Discard the upper broth layer, dilute the lower bacterial cell in normal saline, prepare McFarland 1.5 turbidity and match the turbidity of your bacterial cell using a spectrophotometer. Then dilute to desired concentration of bacterial cell as needed. Thank you

[@EelasMusic]

@@MicroChemsExperiments can I use the distilled water instead of saline for the serial dilution?

[@MicroChemsExperiments]

@@EelasMusic using diatiiled water for dilution in microbiology is not a good practice. You should use normal saline.

[@EelasMusic]

In first question,for 1000 CFU/ml I need to put 0.1 ml of bglb into 0.5 ml of saline right,but that volume is not enough for me,so what can I do to increase that volume without changing the value(1000 CFU/ml)

[@EelasMusic]

In first question,for 1000 CFU/ml I need to put 0.1 ml of bglb into 0.5 ml of saline right,but that volume is not enough for me,so what can I do to increase that volume without changing the value(1000 CFU/ml)

[@Ankitsingh-bq9bu]

Thank you mam

[@MicroChemsExperiments]

You are welcome

[@uchechukwucynthia8450]

Why do you have to use peptone sugar solution for another 1st and 2nd trial instead of using the waste water

[@anshulchoudhary7238]

please make a video for Vibro cholera and Listeria monocytogenes.

[@MicroChemsExperiments]

Noted. Ok we will make.

[@GloryOjimaIsaac]

What other media can replace VBRL

[@ilkhomrakhmatullaev7768]

Hi, I have a quation about Total coliform count! If I have 26 coliform in 1st trail and 0 in second trail, How can I count Total coliforms? (26+0)/2=13, 13*10=130! Is it true answer? Thanks for your answer!

[@seloumabio640]

Normally we count only those who have a number between 15 and 300 so the one with 0 is not countable

[@kurohi9818]

hello can i ask a question can this method be used to test filtered water, to use it as one of the test conducted to test a filter i made?

[@MicroChemsExperiments]

Yes. You can use

[@kurohi9818]

@@MicroChemsExperiments thank you

[@mohdabrar2839]

thanks

[@MicroChemsExperiments]

You are welcome. Stay with us.

[@haiderkhan3062]

Can you make vedio on M Endo agar media preparation and calculation

[@MicroChemsExperiments]

Noted. Stay with us.

[@shazekorcari4922]

Can you replace Endo Agar with VRBLA ?? Thank you

[@haiderkhan3062]

@@shazekorcari4922 Which organisms grow on this VRBL

[@hemedimshanafihiri5371]

Can maconkey used in determination of faecal coliform in food sample which is in solution form

[@MicroChemsExperiments]

Yes. You can use

[@MounikaKinnera-x4m]

Mam why don't we use macconkey agar for coliforms

[@MicroChemsExperiments]

VRBL agar medium is more specific for coliform

[@foodiekamini299]

Can u plz tell me if I have done testing of E.coli on Tryptone bile X Glucuronide medium and after incubation I have found yellow growth on my plate ,so which bacteria is this E.coli or something else????? Plz reply

[@MicroChemsExperiments]

E. coli gives Blue colour colonies on TBX agar medium. So, other gram negative bacteria may give yellow colour colony. So you have to go for the identification tests to identify that bacteria

[@saniketbhosle3390]

Is this technique applicable for food products like milk?

[@MicroChemsExperiments]

Yes

[@gloriagrey5184]

what is the standard values that the water is safe to drink.

[@MicroChemsExperiments]

The maximum permissible limit of MPN in drinking water is 2.2 per 100 ml of water, as recommended by WHO

[@madinimallika9367]

Mam please culture media preparation

[@seloumabio640]

Why did you add the medium for a second tima ?

[@MicroChemsExperiments]

To confirm isolated coliforms

[@poojabondre3016]

MPN method bataiye TC,FC,nd E.Coli

[@MicroChemsExperiments]

Pooja Bondre, Noted. We will work on it soon. Stay with us.

[@poojabondre3016]

@@MicroChemsExperiments okk...

[@kanukanu4141]

Can we use lactose broth for enrichment than pouring in vrb

[@MicroChemsExperiments]

For Total Coliform Count, don't go for the enrichment. Enrichment is only applicable for detection method.

[@abdullahmohamed2027]

thanks in advance for this informative video ,but i have concern if the number of colonies is less than 10 cfu and i analysis food sample as cocoa powder what result i will write in report absent or not detected or less 10 cfu or tltc and what in case higher than 150 cfu also if no colonies detected but i should mention the iso standard i follow appreciate your support

[@MicroChemsExperiments]

Mention all your dilutions. Which lowest dilution you used to inoculate? Mention also the count of each plate.

[@Azmo477]

Yeah, there's a Iimitation in this procedure when it comes to detection threshold. The best result you'll be able to provide is

[@MicroChemsExperiments]

@@Azmo477 you are correct. Thanks for the explanation.

[@KashifateOsseni]

J'adore la vidéo svp 🙏 pouvez vous faire une version en français pour nous

[@MicroChemsExperiments]

Thank you very much. Unfortunately at this moment we can't make videos in French. You can use translator or select french subtitles while playing the video

[@ramkrishansharma6717]

Sir bacillus cereus ki video hai kyaaa

[@MicroChemsExperiments]

We will make it later

[@ramkrishansharma6717]

Ok sir ​@@MicroChemsExperiments

[@ladymex07]

Would you have a pdf version of this?

[@MicroChemsExperiments]

We do not provide any pdf

[@gloriagrey5184]

we get the water sample to the deepwell.the result is 8.0 MPN/100ml. is this safe to drink?

[@MicroChemsExperiments]

The maximum permissible limit of MPN in drinking water is 2.2 per 100 ml of water, as recommended by WHO

[@sinatryaahmaddwifa301]

Peptone salt solution is for?

[@MicroChemsExperiments]

Diluent for Dilution of sample

[@swathikumar100]

Using 1ul or 10 ul Inoculating rod used in microbiology.

[@MicroChemsExperiments]

Your question is not clear

[@gajahmada9665]

what is the name of this method ?

[@MicroChemsExperiments]

Enumeration of coliform from food and feed samples.

[@aak-lm5ln]

@@MicroChemsExperiments this is method not for water??!!

[@MicroChemsExperiments]

@@aak-lm5ln Yes. This method is also available for water samples

[@aak-lm5ln]

@@MicroChemsExperiments but it is Approvalin food.

[@aak-lm5ln]

@@MicroChemsExperiments is there any method to confirm coliform in water with only petri dishes without tubes?

[@Wt520lyn]

Hi, I would like to ask, if my sample is a food, does the steps still apply?

[@MicroChemsExperiments]

Yes. The same method will be applicable for any kinds of food sample

[@Sunnycookies]

Can we apply same method to potable water? Also can we run positive and negative control?

[@MicroChemsExperiments]

Yes. You can

[@surampudiraviteja5089]

Do you have Instagram page

[@MicroChemsExperiments]

Nop

[@kanukanu4141]

How much ml of lactose broth u took for confirmation

[@MicroChemsExperiments]

10 ml

[@glacymaejacinto9451]

What sample has the highest number of bacteria?

[@MicroChemsExperiments]

It is difficult to say because bacterial load depends on sample type and the environment. You will find large number of bacteria in spoiled food sample and in waste water sample

[@glacymaejacinto9451]

What if you serial diluted a water sample and in 1:10 you have 63 counted colonies and its number of bacteria per ml is 630, and you also have 1 colony in 1:1000 and its number of bacteria per ml is 1000. What dilution has the highest number of bacteria?

[@MicroChemsExperiments]

@@glacymaejacinto9451 The plate with 1 CFU should be considered in the colony counting. Count the plates with 10-150CFU per plate for coliform and calculate the total CFU of your original sample.

[@glacymaejacinto9451]

@@MicroChemsExperiments thank you for the info! 🤍

[@MicroChemsExperiments]

@@glacymaejacinto9451 you are welcome

[@gsreekanth7150]

What is the limt of range

[@MicroChemsExperiments]

Detection Limit is 1 CFU/ml

[@s.d.heavyseafoodprivatelim3541]

why add 4 ml of extra violet red bile lactose agar

[@MicroChemsExperiments]

The ISO method said so

[@salmahshahab8146]

if there is no gas in the BGLB medium, how do we interpret the presence of total coliforms? or what is the relationship between the confirmation test and the number of colonies in VRBL?

[@MicroChemsExperiments]

Coliform must produce gas in durham tube. If gas production is failed the interpret the result as the count is less than 1 CFU/ml

[@ZAKIUR.]

Make for mold culture

[@MicroChemsExperiments]

Noted

[@bokulhossain5771]

make a vedio for ice-cream microbilogy test!

[@MicroChemsExperiments]

Total coliform count of ice cream is same as water. If you want other parameter test for ice cream, please mention that parameter. Thank you

[@bokulhossain5771]

Salmonella spp. Staphylococcus spp. Pseudomonas spp.

[@MicroChemsExperiments]

@@bokulhossain5771 Noted. Hopefully we will start working with your mentioned parameter soon.

[@migueth2787]

@@MicroChemsExperiments espectacular :)

[@MicroChemsExperiments]

@@migueth2787 Thank you. Be with us

[@deeppandit3807]

Ma'am please fecal coliform test procedure bataye.

[@MicroChemsExperiments]

Deep Pandit, Noted. We will work on it. Stay with us.

[@sinatryaahmaddwifa301]

265/ml is 26500/100 ml right?

[@MicroChemsExperiments]

Yes.

[@safaafrin5391]

Put up lot of coliform test pls

[@MicroChemsExperiments]

Sorry I didn't get your request. plzz clarify

[@safaafrin5391]

Water analysis test

[@MicroChemsExperiments]

@@safaafrin5391 please mention test parameter.

[@safaafrin5391]

@@MicroChemsExperiments like aerobic colony count, listeria sp, clostridium perfringens, etc..

[@MicroChemsExperiments]

@@safaafrin5391 noted. We will make video for it. Stay with us.

[@abhipal8721]

Hindi me batao

[@MicroChemsExperiments]

Sorry, we use international language.

[@ArtworkShalini]

Are you a teen? Please don't use such an accent.... I'm not able to concentrate 😂

[@MicroChemsExperiments]

Artwork Shalini, I'm not good in English and I'm sorry for that. Please mute the audio and take help from the videos and subtitles.

[@ArtworkShalini]

@@MicroChemsExperiments Oh sorry...I didn't mean to hurt you... Anyways, the video was good. The important thing is that it was informative.

[@ArtworkShalini]

@@MicroChemsExperiments and I wanted to ask, what is MPN/100ml...I mean what is the full form of MPN?

[@MicroChemsExperiments]

@@ArtworkShalini No no I didn't mind for your comment. Your findings will help me to do better in future. I will try to improve my accent in future. Thanks for the comment.

[@MicroChemsExperiments]

@@ArtworkShalini MPN is Most Probable Number. MPN/100ml means Most Probable Number of microorganisms in 100ml of sample

[@gatkuothyuot6807]

please thank for microbiological video this is wonderful lesson you make my experience great again.

[@MicroChemsExperiments]

Thank you too. Stay with us.

[@meenageeths9730]

Thank you so very much

[@MicroChemsExperiments]

You are so welcome