mRNA: Removing Bottlenecks in Process Development and Manufacturing

[@MrMyfra]

How did you get pDNA from mRNA? was it by reverse transcription, followed by cloning the cDNA into a plasmid vector, such as pmRNAxp? and then use the plasmid DNA as templete for in vitro transcription? these are not clear from this talk!?

[@SartoriusBIASeparations]

Hi and thank you for your question. As shown on the slide, plasmid DNA is isolated from E. coli, then linearized using a restriction enzyme. Linearized plasmid DNA is used as a template to produce mRNA in IVT reaction. mRNA is then purified from IVT as described in the webinar. Plasmid engineering (cloning) is not a topic described in this webinar. In case of additional questions please contact us directly at: [e-mail]