Do you Have "Clean Spawn"?: Wet Rot, Hidden Contams, and How to Salvage Bacterial Spawn!

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In this video, I go over common bacterial contaminations in mushroom cultivation. This is to help people see if they have any potential contaminants in their spawn. I primarily go over wet rot and hidden contaminations, plus how to go about fruiting/salvaging such spawn.
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Sage
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[@Mycophilia]

Hitting the *LIKE* and dropping a *COMMENT* on a Mycophilia video has been known to increase flushing power to the MAX! It's true! 🎉🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄🍄👽🛸 BEST GENETICS, code "Mycophilia" for 10% off at checkout: tinyurl.com/ITWMycophilia Patreon: patreon.com/mycophilia Affiliates at: linktr.ee/mycophiliaofficial Mentorship and Genetics: [e-mail]

[@jrewing3164]

I think I'm just going to clean all my stuff out start fresh with a new spore syringe it's been awhile I need to get some fruits

[@ismaelrodarte876]

Hey sage quick question I’ve recently had a problem where my jars colonize everywhere except the tops of the grain they just reach out there and seem to stall any idea on why this might be? And is there anything I can do to prevent it from happening again thank you very much very grateful and thankful for your videos 🤝💙

[@kroketet]

Great vid

[@unicorntheory312]

Hi sage, I need grain spawn bags, do you have a referral? Thanks

[@GarnettRiverProductions5836]

your Canadian? eh 🍁

[@Humboldtcounty707]

The jar that won't break-up is really good info! If your mycelium doesn't break up easily then you're going to have problems. Took me a while to figure that one out.

[@brutallyhonest6103]

I recently broke up couple spawn bags all of them were kinda hard should I be concerned?

[@SketchybrainD]

Ah man, My first grow I did 10 jars and I didn’t get one that was completely clean. I was able to salvage enough to do one tub of gt And it worked. Got a bunch of really small ones the first flush, I was so happy it worked. It was mss straight to grain and I wasn’t very good at hydrating the popcorn at all. Plus I don’t think I did even a full 90 mins.

[@Vodafone16v]

Duuuude!!! Just struck GOLD finding this video!!! Been struggling with bacterial riders for a whiiiile now. Basically have narrowed it down to my LFH filter been saturated, and every "open jar" agar/grain transfer I do in front of it gets bacteria that I later transfer to tubs and get Trich. Really frustrating cuz the myc looks "OK" but at the end of the cycle, all that effort and resources are lost. But, looking at your video I've learned that they may ALSO be embedded bacteria coming in the genetics purchased. 🤔 Cheers man! Keep doing these videos! One about possible origins of such contams/bacteria would be nice!!!

[@Mycophilia]

Thanks man, talk about serendipity. I’m currently editing a video where I briefly talk about one of the sources of such contams. Should be coming out today!

[@kevandgizmo917]

This video has answered so many questions for me and new avenues to pursue which I thought were ended, thank you for this

[@ZombieSnax42069]

Did it scare you away or make you spend 250$ on cleaning supplies and protection ?

[@Somecallme_Hud]

Thank you for putting this together.

[@Mycophilia]

My pleasure Hudman, glad you enjoy!

[@rebeccaambrosino1191]

Love this video content. Very helpful. Thank you so much! Subscribed!

[@Mycophilia]

Thank you 😊

[@bootybootylove]

I’m new like Joe Dirt, but vids like these are super helpful when getting into something new. Learning the different failure modes and what you can or can’t do about them is so important to any process. Very much appreciate the knowledge, the prime examples, and the information! 🍄

[@Mycophilia]

Thank you Mark 😊

[@kristhomas6474]

Thanks for the video. Very informative and helpful. I appreciate it Im a beginner and having lots of trouble and videos like this one is invaluable for me

[@Mycophilia]

Glad it was helpful!

[@ronnienoneyabusiness2844]

I dig your channel man...doing gods work

[@Mycophilia]

Thank you 😊

[@mj_awesome8925]

Amazing video, very informative :D Mush love

[@Mycophilia]

Thanks MJ 💜

[@charlesdarwin4780]

Very informative, thank you!

[@oldboots75]

very useful info 👍 cheers Sage

[@Mycophilia]

Glad it was helpful!

[@joeby5438]

Thanks for this content, its hard to find information on hidden contamination, but it's quite common for me! One idea I wanna share with you @Mycophilia: I remember Mycoplasma are smaller then other bacteria and prefer to stay intracellular, not intercellular. Maybe they are your hidden embedded contamination. Did you try growing this kind of contaminated mycelium on Agar-water? all the best!

[@Mycophilia]

Certainly could be the case. As for water agar, yes, and ime it does nothing for embedded contams unfortunately

[@andyshelly3473]

i learned something new , ive had this . at the very leading edge of the mycelium on agar you can see a faint hays . i have a fruit on the petri dish , so im going to collect the cap and make a new spore print .

[@ovrjoyd]

Excellent and informative content 🤗

[@Mycophilia]

Thanks Ovrjoyd 💜

[@DocR16]

I'm in the process of building a professional lab, so if you want to keep me in mind towards the end of 2023, I can run some tests on that one spawn jar that you can't identify the mold/foreign spores that keeps ruining your grow

[@FederalBeureauInvestigations]

FBI

[@thelvadamee3767]

What is blud wafflin about

[@user-py7rf7gz5c]

Have u tried blastinguvc sterilizing light myco survives the bactia is bleached works best on colonized agar and liquid culture

[@kingmetroplex]

Great video ❤

[@ianbabineaux2050]

Finally a video with good information.

[@Mycophilia]

Cheers thanks for your comment 😊

[@gawdzalien2811]

One day I'll be ready for this

[@stevenmoody-MoodyHivesandHome]

Awesome video man.

[@Mycophilia]

Thanks Steven 💜

[@Unclemushlovin]

Cheers new channel and new to growing mushrooms good info thanks

[@Mycophilia]

🍄 💜

[@peppergrowmienola4850]

Good info. I have dealt with a bit of contamination over the years.

[@1871corporationUSA]

Use water agar. Or cut out a channel in your agar so the mycelium can escape the bacteria by jumping the chasm. Cleaned some s.n.a.p.e culture up via these two methods in one transfer

[@Mycophilia]

You were able to clean up embedded bacteria with water agar? Or regular bacteria?

[@MrPro-hg8dd]

Awesome video very helpful thanks bro

[@Mycophilia]

Thanks for the comment, glad you found it helpful Mr. Pro!

[@melissalovesnature3370]

Great video! Now I know why I keep getting failures with my latest batches. Time to go back to spore.

[@Mycophilia]

Thanks Melissa for the comment!

[@sunnydaze5721]

Hi❤ Such a great video for a beginner Ty xoxoxo 😊

[@sethbaker5261]

I'd love a video on how to use a microscope to verify grain spawn. I've seen very few 'scopes while perusing KZbin myco tutorials. Also, thank you for the tip regarding pressure cooking contaminated grains. I've been driving a mile away to dump, still obviously bringing spores home on my clothes.

[@kaandemirkiran2954]

my fav youtube chanel so far

[@Mycophilia]

💜 thanks 🍄

[@milwaukeebuds]

Thank you! 🫡

[@beentheredonethatoriginals5673]

Glad I found your channel. I have a year old Oyster jar of spawn that is tightly grown in and has several tablespoons of liquid in it. It was pushed to the back of the fridge and forgotten. I'm gonna give it a shot, thanks for the info it looks like it might not be contam after all.

[@Mycophilia]

That sounds like bacteria to me but I’d give it a shot anyways!

[@Mycophilia]

Glad you found my channel, hope you enjoy!

[@TheStoneMan.Official]

Cleared up a lot here good timing on this topic!!! Thank you so much Sage always learning more from you!

[@Mycophilia]

Glad it was helpful!

[@AndreaDingbatt]

Thank you very much for sharing your thoughts and knowledge!!👍 Much Appreciated,💯 Andrea and Critters. ...XxX....

[@Mycophilia]

🍄 💜

[@ericwilkerson4912]

I'm really glad to find this video today because I have a big tub exactly like that

[@Mycophilia]

Glad to hear that!

[@looneycrow7978]

Well done video

[@Mycophilia]

Thank you, appreciate it!

[@sacacorchosbarravel4909]

Been there, done that. Great vid..

[@Mycophilia]

Thanks!

[@martinmadrid3192]

Mold infects plants and fungi systematically, it has me question if some bacterial infections aren’t the same. Thank you for sharing so much information 🙏

[@Mycophilia]

Thank you for watching :)

[@adammiller6747]

Bro❤❤❤❤ i fucking love yer plates😊 absolutely gorgeous

[@DanSetzer]

You got your tea right beside bacteria colonies 😮😮😮❤ keep it up watch that tea xd

[@PovertyStudios]

Trich kinda takes over in growth quickly, if it's spotting light green it's penicillium, trich spreads much faster and has a forest green color when matured, penicillium takes longer so you can let it go a little longer before you have to toss it out, gives you chance to get some fruits out if you're at that point that is. Love the vids brotha!!! Keep fighting the good fight!!! 🦍🙏💪🏿

[@Mycophilia]

👍 👍

[@nattybizzle]

You can eat contamed fruit?

[@PovertyStudios]

@@nattybizzle depends how contaminated I guess lol, you mean like if you picked fruits from a tub that was contaminated? Or do you mean if the fruit itself has contamination on it? Either way best case scenario is you get diarrhea, worst case is you get sick and die lol but i would use the fruit from a contaminated grow to try to clean up into a healthy culture for future purposes if that's what my objective was at the time.

[@VincenzoC749]

I loved the video, but I am not really sure what to look for. I see the little yellow metabolites, and the fluffy white jars definitely look strange. But I think I just need to do more research on contam issues to get a better free asp of the possible mycelium pitfalls that you’re likely to encounter, compared to healthy growth.

[@Mycophilia]

After a few grows you will begin to see what healthy mycelium looks like and unhealthy mycelium looks like. It’ll all fit together so have fun while you’re at it!

[@Lucas-jp7uz]

I have a tub that looks like the jar you said would become trich. I also think my tub is going to turn to trich, after seeing this though I am hopeful because it's passed the time other tubs usually turn green. We will see though, probably just jinxed myself lol

[@kiowhatta1]

I find Trich has a greyish hue, colonises very quickly and looks like cotton balls, but mostly it doesn’t have the beautiful alabaster white shiny complexion of mycelium.

[@Mycophilia]

A lot of trich is gray, but they also go super white just as much. Waaaay whiter than mycelium, usually before turning green.

[@buybitcoin69]

Dope!

[@Mycophilia]

[@kiowhatta1]

Why one would bother with PF tek anymore is beyond me. But each to there own. First I had to pressure cook the jars, drill holes then squirt 2ml of liquid spawn into each jar. I did 4 jars, lost two cakes to bacteria, then put the other two in a tub with perlite on foil. (It took a month to colonise the jars btw). One cake produced a few fruits then that was it. I’ll never do it again.

[@thesupreme7815]

What do you do now

[@MoonHaze_exe]

Super helpful video thank you so much! I've heard that the "infused bacteria" in the mycelium can be a sign of a bad syringe, can anyone confirm if this is true?

[@Mycophilia]

Not necessarily a sign of a bad syringe as you can get it thru any medium. But dirty spores will greatly increase likelihood of getting contaminated mycelium.

[@metamike2012]

That explains a lot. I have to cut my spawn out of the jars. There's no breaking it up. All my agar looks great ! At T -7 now starting to get ropey but put to grain it stalls out . If I shake it, it doesn't recover. Time to top fruit for some new spores. Thanks !

[@Mycophilia]

🍀 💜

[@kgproductions273]

7 transfers?

[@stellastarfield1111]

what i gotta go down this rabbit hole

[@mikeinnj4424]

Great video! Will keeping my grains at a slightly cooler temp help deter the bacterial growth and allow the mycelium to overtake it?

[@Mycophilia]

It’ll help deter bacterial growth, but if the bacteria’s already ‘triggered’ then it won’t make much difference as the mycelium will always lose eventually

[@johneywangers1425]

I'm when I can get a set up figured out I'm gonna look at mutations with variable mutates like bacteria uv exposer ect...

[@jamesguiberteau6607]

Good to know

[@allseeingguy5485]

I had no idea that the mycelium itself could be contaminated. I had this problem for many runs and took a break because of it.

[@Mycophilia]

Something that isn’t talked about enough!

[@danwilkinson2797]

Those overlaying myceliums often look quite funky such as the ps. Nat

[@Mycophilia]

I got some real overlay on a mckenaii tub

[@ebtsoby]

I had a box that formed hard nodes all over the surface and wouldn't fruit, never seen something like that before, smelt great tho even when I broke it up and threw it away, wonder if it was some sort of embedded bacteria, I got rid of all the grain cause I tried to fruit it 4 times and gave it a month each time no success

[@ineffably_described]

Thank you! I just spawned a bacterial B+ to a shoebox with a 1:1 ratio. It looks great but I will have an eye on it. Had to scoop out the grains but they smelled wonderful still.

[@Mycophilia]

Best of luck! 🤞

[@user-xr5cz2mr4v]

@@Mycophilia If you have a few bacterial spots on the outside, cant you rub the spawn until its sides are rubbed off and just bulk the middle part, which should be clean because it has its stable immune system.?

[@1871corporationUSA]

​@@user-xr5cz2mr4vno, the bacteria transfers via water, humidity. Culture has to be cleaned up via water agar, or multiple transfers on regular nutrient agar with chasms sliced into the agar to impede bacterial spread

[@Mustachedmyco]

Heey homie i Had nothing but problems with fliped lid tek!!! 😞 Switched to ball brand plastic lids with 1 , 1/4 inch hole , covered with mirco pore tape i use 2 layers! Grab some low nutrient antibiotic agar if ya haven't already those plates will clean up 🔥🤙 🍄

[@Mycophilia]

Nothing to do with flipped lid here. But I appreciate your comment nonetheless. Antibiotic agar is also not a cure all. Most times, it will only accomplish suppressing the contamination temporarily, but once put to grain or a non-antibiotic plate, the bacteria will be back.

[@pequiventania9658]

After scaling up my grow I got a lot of bacterial contam, still trying to rule it out. I was using popcorn, never had a problem before while using jars, but when I moved to bags I got into problems, all of my contams are bacterial, not a single colorful mold :( Now im using millet, perhaps it will be easier to achieve clean spawn.

[@RickL555]

Great vid man. I stopped using WBS because some bacteria can burro deep in low grade grains and can survive the pressure cooker. Keen eye on that particularly deceptive agar plate. I wouldn't have caught that one in a million years. Patches of mycelium that grow little spikes are another sign to look out for. Keep up the good work, homie.

[@Mycophilia]

Thanks, appreciate it 💜

[@Animal-Reaction-Clips]

So what is the best thing to use then? Thanks

[@consolechips]

If you soak the grains for 48 hours you can germinate the bacterial endospores and this way they will be killed in the pressure cooker.

[@RickL555]

@@consolechips Right on, Thanks. That makes perfect sense.

[@GNARLOUSE]

​@The Eye-Rollers Guide to the Galaxy haha just watched your vid about this! Embedded bacteria huh? Well guys live and learn...sigh. What in Your guys opinion would be the bullet proof medium to avoid all this? Thanks to both of you

[@mikesw3ll153]

So i am letting my agar plates grow . I plan to transfer them to jars and substrate in a tub . My question is i just recently noticed that my ceiling has a few black colored mould on the ceiling .if im careful and do my air transfer outsude is it ok to grow inside of a tub if there is some mould on the ceiling ? Is there anything i should or shouldn't do ? This is my first time growing . Please and thank you

[@ST1CKF1SHGAMING]

Ive just started out and did as much of the sterilisation I could with everything. I did an oyster batch but looked like white fuzzy mold starting after a few days so got rid of it also those random white dots in various places. Then I redid the process trying to be even more careful with another LC syringe (GT) I got in a pack. Was as sterile as i could be with alcohol, etc but on rye and oven baked at 80 deg C for about 2.5 hrs since i dont have a PC yet. Made sure to squirt down side to monitor, after 3 days I can see white either whispy or early signs of white fuzz. Should i leave it and monitor or perhaps toss it while its pretty early? Appreciate the content and learning a ton so far.

[@ST1CKF1SHGAMING]

Ok ignore previous comment I watched your rhizo video and my mind is at ease now. I wish we could show pics here so you can see how my grows are coming along thanks to your advice. Keep at it love the info ❤

[@Mycophilia]

Glad to hear that 😊 best of luck!

[@its--_--me]

Apologies if you've already addressed this in a previous video, but when fruiting truffle producers, will the fruits be more potent the longer i let the spawn colonize? Or as soon as the jars is colonized(little to no truffles forming) spawning to fruit will be the same potency?

[@MattRoadhouse]

the fruits are tiny and potent as it is. Recent run was 6 months truffle then fruiting trays with peatmoss casing, but I'm going straight fruiting for Tampa / Mexi this round. Will see if they are in the same ballpark.

[@dankfinch]

I regularly get very strange mycelial growth with certain varieties that looks very much like @6:06. But very rarely have contamination until the third flush when the bulk sub is ready to be tossed. Its quite possible in my opinion that you have poor genetics leading to zero fruits, and eventual contamination after months trying to fruit. I think an important thing to mention is rhizomorphic mycelium, and tomentose mycelium and a combination of the two. Some of those jars look more like tomentose growth in combination with bad genetics. But in any case do what works for you and I hope you can get some new genetics from that variety and create a stable performer.

[@lexolotlgod]

Thanks. I came here with BRF jars that look just like that. Very fluffy and cloudlike. Other places online have suggested they mycelium has pulled away from the jar, leaving a low-oxygen area, and that this is the same fuzziness that can occur at the base of mushrooms in monotubs.

[@CarolynsLoveRickshaw]

How do you recommend disposing of contaminated spawn? Thanks for the great content.

[@Mycophilia]

kzbin.info/www/bejne/fHvQiYmCjtVsa5Y

[@Animal-Reaction-Clips]

Chuck in in your neighbour dish

[@jrewing3164]

I have the same problem when I put him to fruit I get a little bit but just stalls out

[@conormarie4310]

interesting I've probably come across that without realizing what it was.

[@MitchellPartaka]

Thanks for the post. I have some serious thinking to do. Should you shake or not. Lol

[@Mycophilia]

Shaking is good, shaking doesn’t cause contams, it just shows you that contams were apparent in the first place if they do not recover after a shake. Shaking basically saves you time in two ways: 1) it lets you know if you have contam earlier on if it doesn’t recover from a shake 2) it spreads mycelium everywhere so it speeds up the process itself if the mycelium is healthy

[@MitchellPartaka]

@@Mycophilia Thanks for replying.

[@foxybuddy]

What exactly the smell end up like if that's not contaminated? I've done my first try, the mycelium bottle smelled like wine, quite strong when breathing in, suppose I should wear mask before opening the lid

[@patswayze7359]

I always run into the bottom of my jars getting wet mold.idk if it pack the grain to tight or what

[@TheQuixoticRose]

Omg that really white mycelium is something I thought was healthy and it's true right after the first flush they contam 😭 or even during the first one and I only get two fruits

[@ebtsoby]

never heard of embedded bacteria, I thought if it was clean on agar it's clean period, thanks for the vid

[@Channel-tl3xz]

Those "embedded bacterial" jars are not actually contaminated. I think he's mistaking crappy genetics. Those don't look bad at all. There's no "tell tale" sign of anything in those jars

[@bryannakvinda8411]

I have been dealing with this and I believe it is my spore source going to swap and see if I am having contam issues from room or hood

[@Mycophilia]

Dirty inoculant is a prime cause for embedded contams 👍

[@bryannakvinda8411]

@@Mycophilia yeah I know but I wanted to give a local company a chance looks like I will be putting in an order and making a phone call to my trusted bank

[@ows1993]

Hey great video! I am doing LC's for my tranfers to grain and it seems i get this thick white mycileum along the sides of my jars, but the center is pretty easy to break up and not thick at all. Does that sound like that bacteria or just mycelium ?

[@Mycophilia]

Thank you, if it’s easy to break up that is a good sign that it’s not contaminated. The only way to truly tell if it is clean is to just try using it.

[@ows1993]

Thank you for the quick response, much appreciated. I will se how they work out 🙂

[@WhoDeyWildcatsReds]

Question, were those white jars of the compressed myc started from what looked like a singular culture on an agar plate from spores? I think people get germinating spores mixed up at times. Spores can germinate and grow by themselves. They only need to meet up later with other germinated spores to go on and grow healthy mycelium and eventually fruit. This may be the problem with that myc. I'd try introducing another sample of myc that came from the same strain and see of they marry 🤷

[@Mycophilia]

No

[@biker_one208]

Üblicher Weise fertigt man aus dem Petrischalenansatz erst einmal Flüssigmycel an. Dadurch lassen sich viele Probleme mit Kontaminationen vermeiden.

[@DangerRussDayZ6533]

Every jar I've ever tried to spawn has turned into those thick bacterial contam but fully colonized / smells fine jars. I've been thinking it's the WBS I've been using and/or the holes in my modified lids aren't large enough.

[@Enochulate88]

You should see how small premade holes are. Like a screw driver or nail size

[@Enochulate88]

You should see how small premade holes are. Like a screw driver or nail size

[@renasdupont2806]

would all of those take over the myco a month after innoculating mss or is it always much faster

[@beekeeperd.7656]

Is this all you got? lol. I was hoping to get nightmares from a mutant Blob consuming the city. I grow Tric just for fun (or so I tell myself). Sorry but my contaminants would eat yours for breakfast. Aspergrillis is my homeboy. It's not a grow till I see happy knats greeting me! But seriously folks... Good stuff. I see wet rot too often in my oat grain jars. Thanks for good info!!

[@Mycophilia]

Oh no, not the aspergillus! Thanks for the comment Beekeeper!

[@burgerbobbelcher]

You said "these two guys are full of truffles". What does that mean? Is that an insider term for some kind of contaminant I haven't heard of? Or have you actually discovered a way to cultivate truffles?

[@bracedforpleasure]

Have you looked into hydrogen peroxide flushing your substrates and grains before inoc or bulking?? Also with certain contam caught early enough it can treat bulked tubs by spraying and wiping up.

[@Mycophilia]

Nope peroxide should never be used that way.

[@bracedforpleasure]

@@Mycophilia I'm genuinely curious Why is that?? I can't say I've heard of any negative effects from either method. But there's a lot I don't know so any help is much appreciated.

[@fredrikvang]

Very interesting, but what makes you think the jars with the white mycelium are contaminated with bacteria, or even contaminated at all? Have you done some tests that support that notion? Might not something else actually be going on? (BTW, next time why don't you slice the white mycelial mass into 1.5-2 cm 'breadslices' with a knife and try putting them btw a thin 50-50 CC-Verm bottom and thicker 50-50 CC-verm top layer and see what happens. I suspect breaking up spawn that looks like that might not work very well.)

[@Mycophilia]

They are 💯 contaminated, once you’ve grown for some time you know what’s contaminated and what’s not. I have listed a number of reasons on this video, the primary ones being the extremely thick growth that makes it impossible to break up, along with all previous attempts with this culture always ending up with the same green mold, at least those that have been spawned to bulk. The ones that have not (bottle fruited), have not fruited in 2+ months of fruiting conditions, in addition to growing very strange mycelium masses after the first month, a hallmark of contamination. As for your idea of slicing, a better method exists called jar/top fruiting. It is better because you don’t need to break up the mycelium at all. Hope that helps.

[@Enochulate88]

By the smell lololol

[@gelhope5510]

​@Enochulate88 he said it didn't have the smell.

[@jamesrapkins4935]

Thanks for the good oil! i have a similar situation with a H.PES genetic... i was sure its carrying something, but i cant see it on agar. there is a new tek available that is being tested in the community btw. Rit dye agar.... the other option would perhaps be water agar ?

[@Mycophilia]

I’ll make an update if anything works!

[@Animal-Reaction-Clips]

Why not just keep it simple, it's fungus it'll grow on shit if you leave it alone.

[@Shitstain951]

@@Animal-Reaction-Clips lmao you’re one of those morons. Okay then explain how one would “keep it simple” when their cultures are contaminated? Dirty straight from the vendor?

[@kushgardenr1652]

I had a few fully colonized jars of grain and they didn't appear to have any contamination. but there was a little bit of water in the jars after they were fully colonized. But I opened them to spawn them to bulk and they all smelled good like fresh mushrooms. Do you think they should be fine ?

[@Mycophilia]

Probably, only so much I have to go on with that description but I’d definitely spawn it either way

[@dankernuggets7]

You got any tips for my grow bag smelling like yeast? I'm on my second flush and have lots of fruiting pins. I'm assuming not enough FAE or it's too moist? Thanks!

[@DoseofScienceDoS]

If your bulk is going bad then you have 1) Not fully sterilized (longer higher temps required) Why does this happen???? Elevation has an effect on temp. 2) You introduced a contaminant (Lysol/alcohol and draft hood) 3) Your inoculate is contaminated (do a better job on sterilizing and use smaller wide mouth jars)

[@Mycophilia]

It’s 3, I cover that in the video. Thanks for the comment!

[@1To4IlD]

Where can I get ahold of You, personally? I've got some Grains I'd like You to see so You can possibly help me decide on what it is & the nxt steps I'll be needin to take. Also, THANK YOU for all the GREAT ADVICE & KNOWLEDGE that You KINDLY SHARE w ALL OF US!!! 🤙🏽😎🤝🏽

[@Mycophilia]

Hey 1T thanks for watching and commenting! I currently only do private questions in my mentorship program. Otherwise you can post your questions on my subreddit or discord server (you can tag me with your picture with @mycosage and I will come take a look).

[@1To4IlD]

@@Mycophilia Okay, cool. Thank you!

[@vincentwu2848]

Hey! I think I'm dealing with the same bacteria as you. I've also done multiple transfers to both agar and grain and still am seeing those big fluffy clumps on top. Do you know if it's definitely bacterial, or could it be a mold or yeast? If it's bacterial then perhaps a transfer to antibiotic agar could clean it up?

[@Mycophilia]

Definitely worth a try. I would recommend doing at 2-3 xfers on antibiotic agar, and then 2 xfers on regular agar to make sure it won’t come back. This is because bacteria will always eventually outsmart the antibiotics eventually

[@Mycophilia]

It’s definitely not mold, that much I can say. Yeast is possible as well, although I can’t say for certain.

[@MB-sn8sf]

12:19 in those situations the best best is WATER AGAR

[@Mycophilia]

Unfortunately water agar doesn’t do anything for embedded contams

[@UrMothersLoverr]

look into mgp and try that it should help with your bacterial and trich issues

[@jonrastrick1997]

I'm on my first attempt, and at first, the mycelium was growing beautifully, but today, I noticed a spot of localised green mould near the top of the jar. What would be your advice? It hasn't spread yet, and it would be easy enough to scoop it out with a sterilised spoon and discard, but I'm worried that the whole lot might need to be thrown out.

[@ajhosmer315]

Throw everything you used away. Otherwise you risk everything. Look up trichoderma

[@user-fu3mu2uu6i]

Can you still eat the mushrooms safely if they have bacterial blotch ?

[@fernetstock1]

I think I have the same problem. Transfer through antibiotic agar could help with those embedded bacteria (gentamicin, chloramfenicol)?

[@Mycophilia]

It should, I would recommend doing 2-3 xfers on antibiotic bacteria, then 2 transfers on normal agar to make sure it doesn’t come back (as bacteria will always overcome the antibiotics eventually, so you need to make sure they’re gone for sure before putting it to grain)

[@loganmontes9]

In regards to the bacterial spawn, my grow bag has looked like that since inoculation. I was informed it was “tomentose mycelium”. It’s grown fine and now It’s in its 3rd week of fruiting conditions, not much has changed. I’m starting to get concerned that it may never fruit. What are your thoughts?

[@Mycophilia]

3 weeks and no sign of fruit is very slow. From my experience, I would definitely bet on bacteria being the case.

[@shaqx7039]

are you using a still air box or a flow hood?

[@Mycophilia]

Both

[@knottyinks1]

I’m new to the hobby, currently experimenting with 3 Ben’s tek, all look good, two smell mushroomy one smells sweeter??

[@Mycophilia]

sweeter often means contam, likely yeast

[@kenbourekas420]

I started doing this chasing mycelium into fruit about a year ago and I use the uncle Ben’s method but the last six syringes I received all turned into super green nasty. My guess is that it’s Trichoderma has a very foul smell to it. my first I’d say eight times was very successful. I have not changed my procedures if anything I have gotten even more excessive compulsive about cleanliness. I am guessing that I am getting contaminated syringes. From your experience, is that a possibility?

[@Mycophilia]

Absolutely

[@Braten.111]

I am in the same Situation right now.. Mycelium looks really thick and a lot of White dots and uneven, not flat at all.. but no bad smell at all... I still made a bulk in a monotub, but I don't know if it works.. I have got an orange touch and some dry powder from some jars.. I assumed it to be Penicillin, but who knows!!

[@Mycophilia]

Powder and orange are a no-go

[@Braten.111]

@@Mycophilia yes, but is it Penicillin, because it has no smell. It actually smells fresh?!

[@Braten.111]

@@Mycophilia the Orange is like the colour from oyster mycelium, more white, it just looks like that in the reflection, only slightly!

[@fattymcbastard6536]

I intend to try to perpetually multiply a "ready to fruit" lions mane kit, grown on fuel pellets supplemented with wheat bran. If I quadruple the kit, (3 bags for fruiting, 1 to inoculate the next generation), how many generations can I reasonably expect to produce before I hit a wall of contamination and have to start over? Could I reduce the risk of contamination by skipping the supplementation? On one hand, I imagine the mycelium may not spread as quickly, on the other I imagine the wheat bran may be inviting organisms that would otherwise not infect hardwood. What do you think?

[@HellGod67]

Where can I get the truffle culture and how it's called?

[@youtubee432]

Are there any that are very harmful to human or pet health?

[@Lipi19821]

I would never thought that unbreakable mycelium is contaminated...I am no expert tho.... maybe anti bacterial agar would help... make clean coulture...?