How to Use an Autoclave

There are a lot of products that need to be sterilized: pharmaceuticals, equipment, solutions, medical waste, and even tattoo needles! We use a device called an Autoclave to sterilize materials in the lab. In today's demonstration, we'll show 2 common applications:
1. Sterilize a solution using slow exhaust.
2. Sterilize and dry some empty glassware using fast exhaust.
You can explore our interactive autoclave for free at www.ncbionetwo....
*Special thanks to Meghan Davis, Toby Mapes, and Rowan-Cabarrus Community College for making this project possible.

[@Usedstryker]

An autoclave is an expensive medical tool and should be handled properly. If you are in doubt on what to do, always consult the user’s guide.

[@frederick-nrunkkamara103]

Autoclaves have come along way since I autoclaved media broth

[@ACG099]

Thank you very much... it is really a good video to understand the basic mechanism of autoclave... GOD bless you muffin choc answer to your question is "yes", you would use the same procedure but "fast exhaust" one because you are just sterilizing beakers (which are empty glasswares)... remember... liquid turns solid slowly, so slow exhaust for liquid media's and .... solid melts fast, so fast exhaust for solid things (i.e. instruments or empty glass wares) Regards, Dr. Ammar

[@me-de6hl]

Hello, thank you

[@raishahaddad9705]

I love and supported the people who making this educational videos 😄😄😄

[@jillian3047]

Cramming for a certification exam tomorrow. Thank you!

[@captin__7658]

Jillian What exam did you take? I hope you passed 🙂

[@ilyasjamei1413]

@@captin__7658 hello , are you still here?

[@captin__7658]

Ilyas Jamei yes?

[@vantablack1119]

@@captin__7658 no

[@TomsonGaming]

love educational videos like this helped me alot:)

[@nudratfatima5755]

very easy for us to explain students

[@vatsaldabhi1145]

superb to the point explanation!!!

[@tandy2740]

How do you change the length of sterilization time? And about how long does the entire process take (for autoclave to reach the proper temp/pressure, about 15 sterilization, then de-pressurize/cool down)?

[@funny11744]

Question : the object like an agar bottle - must be kept totally in the steam or just half of them?

[@akhanshamenov8924]

Using autoclave is important when preparing for a surgical procedures. I wonder if it uses more energy than kettle or microwave.

[@SpiraSpiraSpira]

akhan shamenov Yes, an autoclave uses more energy than both. Also most autoclaves are configured to maintain a certain temperature even when not in use so as to make the sterilization process quicker. Even this idle consumption is about the same as a home microwave (1kw or so)

[@Industrialfoodssolutions]

Why not use a universal sterilizer?

[@autoclavedepot1242]

Nice job mate!

[@jjptech]

Thank you for the video. Do you know any FDA or ISO guideline for ussing propperly the autoclave? Thanks.

[@8372cd30]

ty! This helped a lot

[@cebidexpharmaceutical6314]

thanks fast and to the point information...

[@billk6494]

Important Videos For New Tattoo Artist! Take Care, Bill Kearney :-)

[@ilyasjamei1413]

are you a tattoo artist?

[@dainanaorbe6965]

May I know the specifications of this autoclave. Thank you. Planning to buy this kind of autoclave. Thank yoj for the response

[@zahraothman1529]

شكرا حبيبتي💜❤️

[@RynaxAlien]

Is vacuum also used?

[@diao77723]

how does the pressure clean the inside of the containers? surely some extremophiles type bacteria could live on the inside of the sealed containers

[@purugigi]

Considering where those extremophiles live, I highly doubt they'll make their way into an autoclave.

[@diao77723]

i mean on the inside of the bottles being cleaned, their sort-of sealed so i dont know how the pressure can get inside the bottles to be cleaned

[@kiaghods5730]

Diarmuid Mac Mahon you’re not supposed to completely tighten the lid on bottles when putting them in the autoclave.

[@isasamuri1061]

what if i use slow exhaust to streil empty glass

[@ashwini16k]

Thank u so much 🙏🏻

[@chickensataybroccoli]

is it 121F ? struggling here in Aus

[@HammadAsim]

Elouise Gaylard its celsius

[@arlix6845]

isnt inhaling the air coming from the autoclave is bad for us

[@uknowngirl2531]

the embed code doesnt works in powerpoint .pls help

[@angela-xk2ov]

when should I turn off the autoclave? after or before opening the lid once the cycle is done? help please:)

[@lijaucolouiserichard2320]

121 degrees 15psi 15 minutes 121 degrees 15psi 20 minutes

[@nikolebryant7447]

Is there a specific job in a hospital for sterilization using autoclave? I'm currently a nursing student exploring what I may want to specialize in.

[@sparkysmom7149]

You would want to work in a Clinical Laboratory then

[@binnykakkassery1869]

Is this use for retort pack

[@reyakurd8810]

thank you for video

[@bdlibya17]

thanks

[@fvasonline6205]

A good video.

[@PandaReefNguyen]

FUNJEE

[@Ajayjohnson264]

Fun Gee

[@pronglebeing8389]

hahahahahahahahahahahahahahahahahahahahahahahahah

[@Donatellangelo]

These still can't destroy prions! D:

[@karencanaviri1173]

Escape lento - Líquidos y desechos médicos Escape rápido - Cristalería e instrumentos vacíos

[@lethalx88]

Ugh...never sterilize tattoo needles buddy.

[@Fun_tamilan]

வணக்கம்

[@leewilliam3417]

Mmmmnn 😊

[@mominjaved7616]

Good.. But don't use music