This weekend will be the final setup of my home TC setup. 🎉 Pennsylvania winters are my biggest worry; last winter we had -40°F wind chill and everything in my house froze. 😕🥶 Pray for a warm winter, please! 🙏
@Lilyastacio Жыл бұрын
Hi Francisco! Is the first time watching you! I am here to learn! Thanks for sharing your knowledge!!!
@PlantCellTechnology Жыл бұрын
Hi Lily, Hope the shared information is useful for you. Thanks for your comment.
@dragonsimon13 ай бұрын
Have you seen any efficacy differences between cultures with saran wrap and without? The purpose would be to mostly help prevent contamination from mold spores correct?
@PlantCellTechnology3 ай бұрын
Over the years of using the wrap, I have noticed that the media last longer, and I don't have to subculture as often. The contamination rates are also lower.
@dragonsimon13 ай бұрын
@@PlantCellTechnology thank you! I was wrapping all my cultures for a while but when I have so many cultures to do, the time can stack up and be tedious
@PlantCellTechnology3 ай бұрын
@@dragonsimon1 We totally get the pain.
@briansplantlife Жыл бұрын
Nice presentation!
@PlantCellTechnology Жыл бұрын
Thank You!
@febeomnibeepboop636711 ай бұрын
bathrooms are wet, wet is good for molds, molds are a contam risk. probably better not in the bathroom. kitchens have a lot of bacteria so also not the best. a good procedure with stil airbox etc however allows you to work in absolute filth :D the size of the space and thus draft matters a lot in this. watch out with stil air boxes SAB using open flames and cleaning products that are flammable ;), lol okay im still watching and kudos on that demonstration . i did see a procedure mistake here, when you use a S.A.B you want to organize it in such a way that you never have to reach over your work. i seen that hand hovering over the medium going for the burner, it would be better to set it in the center ( the burner also cleans the air btw by burning off any contam, bunsenburners are sometimes used to create a clean air bubble). this hovering over things can cause things to fall from your hands or arms. so always plan out the steps and set up things so you dont have to reach over things. work front to back instead of left to right. i always imagine the center being the place of work and the sides to be the hand moving area and the back corners are storage
@W4lkthroughWizard7 ай бұрын
Dont u have a window in your bathroom? Mine is dry af. You got to improvise if youre doing TC at home
@mikearts6836 Жыл бұрын
How come the media jars you use is not hte one you sell on your shop?
@danieljheelan5256 Жыл бұрын
Thanks a lot for sharing Francisco. Take care while working with flame, cleaning and tools in alcohol. I put fire inside. Also the flame risk to melt the top of the plastic container. Daniel
@PlantCellTechnology Жыл бұрын
Thank you for the concern, Daniel!
@Unkn0wn1133 Жыл бұрын
Do you also do flasking orchid seeds or just tissue culture plants
@PlantCellTechnology Жыл бұрын
Currently, we are just doing tissue culturing of plants. Is there anything specific in which you are interested?
@Unkn0wn1133 Жыл бұрын
@@PlantCellTechnology orchid tissue culture?
@PlantCellTechnology Жыл бұрын
We didn't try orchids in our lab. But, you can easily find the protocol for the plant on the internet as it's one of the extensive tissue-cultured plants. @@Unkn0wn1133
@Hekate-g6r7 күн бұрын
Your English is like a mix of English of a Spanish speaker and a Dutch speaker :) An interesting fusion...
@PlantCellTechnology7 күн бұрын
Our director’s accent definitely has a unique flair, reflecting a mix of international experiences. Glad you found it interesting, and thanks for watching our video!
@pangalang Жыл бұрын
thanks for your great work. i have 2 grams of meta toplin and need to know how to make a stock concentration and the best solvent to use can meta topolin be autoclaved? Can meta topolin concentration be stored over time?
@PlantCellTechnology Жыл бұрын
You can learn about preparing stock solutions for plant hormones here: www.youtube.com/@PlantCellTechnology/search?query=plant%20hormone Add meta topolin in the media after it's cooled down using the syringe filter technique.
@buckbeaksgarden76111 ай бұрын
great questions! I want to know now too :)
@labeenausmani9696 Жыл бұрын
Please tell me the Hardening process
@sweettooth5737 Жыл бұрын
There are a bunch of vids on YT that teach acclimatization for TC. In my opinion, having a good humidity dome with vents is the best way to start.
@sweettooth5737 Жыл бұрын
This is what I use: kzbin.info/www/bejne/gmqai3mQapujnZY
@PlantCellTechnology Жыл бұрын
Here are some videos to learn about acclimation process: www.youtube.com/@PlantCellTechnology/search?query=acclimation Hope you find them useful.
@stonheng9 ай бұрын
Im courious if they got contaminated or not
@PlantCellTechnology9 ай бұрын
We will update you soon!
@nadunrosh10 ай бұрын
wow thats cool
@hbee3688 ай бұрын
Your bathroom bigger than my apartment 😂😂❤
@PlantCellTechnology8 ай бұрын
Haha!!
@geoffgeoff1434 ай бұрын
Tip. Do not use purple metho in a spirit lamp.
@nikkilynick9606 Жыл бұрын
Is there a difference between 70% and 90% alcohol
@PlantCellTechnology Жыл бұрын
70% alcohol is more diluted having 30% of water, whereas the 90% is more concentrated with 10% of water.
@febeomnibeepboop636711 ай бұрын
the higher % decreases the time it sits unevaporated which reduces the time its killing bacteria, it also helps getting in to the cells to have some water in it. be aware tho as alcohol isnt all that good at killing mold spores, unlike bleach solutions
@antoniosanford4675 Жыл бұрын
The next step is for you to build a gene gun!
@sweettooth5737 Жыл бұрын
I'm going to try growing some Japanese red maple seeds in TC this winter. Is the protocol different for starting seeds? I read that tree TC usually uses 1/2 MS amount but no mention of seed protocol. 🫤
@PlantCellTechnology Жыл бұрын
Yes, generally protocols differ based on plant species and even the explant used in the process. We haven't tried the species yet in our lab. But, would love to get some update from you if you are going for it. Good luck!
@sweettooth5737 Жыл бұрын
@@PlantCellTechnology Thank you! I just got three cuttings from my neighbor. I know that I should take them in the spring but he wasn't sure he still be living there next spring. Will give updates. 👍💚