DIY Tissue Culture: Carnivorous Plants

Will there be carnivorous plants in GTA 6?
Join the Plants in Jars discord server! / discord
Although I'm working with a Venus Fly Trap in this video, you could replicate this process with (pretty much) any carnivorous plant. Francisco and I also filmed a Nepenthes tutorial over on the Plant Cell Technology channel (AND we started carnivorous plant seeds in TC! very dope) so make sure to check it out: bit.ly/3TEl7Ki
My favorite place to get TC supplies (use code PLANTSINJARS for 10% off): bit.ly/3NPE5KE
Take the Plant Cell Technology Tissue Culture Master Class (use code PLANTSINJARS for 10% off): bit.ly/3wuubZc
Products used in this tutorial:
• MS bit.ly/3prgxSX
• Gellan Gum bit.ly/3S5z0Am
• Agar bit.ly/3MprY6W
• BAP bit.ly/3MYJ2zv
• IBA bit.ly/3N0cghE
• Snap Lock Containers bit.ly/3wTY0m5
0:00 hi it's PIJ
0:36 Multiplication Media
2:33 New method of explant sterilization! (hype)
7:13 Establishment
9:47 Subculturing + Rooting Media
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
Track: ILY Baby - Dyalla | No Copyright Musi...
Music provided by GameNCM.
Channel: / @gamencm
Free Download / Stream: bit.ly/3BknoRx

[@Helveteshit]

I remember the comment, where you responded about this being possible. And here we are, you are actually doing it!

[@Scooby-Snacks]

So excited you're going to talk about the Venus fly traps. Such a cool plant. 💚 🌱🫙

[@ronrothrock7116]

Venus Flytraps are so cool in culture. Mine lived in culture for over a year without ever opening the container!

[@johnsHobbies411]

@plantsinjars, I have a request. There is a new type of pineapple that's come on the market that's pink. To prevent people from growing their own pink pineapples at home, the developers remove the crowns from the pineapples before they are shipped. Sometimes there is a little bit of crown leaf tissue still alive from incomplete cuts of the crown, but I have been unsuccessful getting any of them to take using cutting methods of propagation. Can you demonstrate how to sanitize and grow a pineapple plant using a crown leaf from tissue culture?

[@Louis-kw6yk]

This would be very interesting!

[@cyr751]

there is a study done called "studies on micropropagation of pineapple" (atawia et al) that tells you their method and protocol for propagating one! you can find it for free online. good luck!

[@Shearedfield]

This is actually amazing! The Venus fly traps are dwindling in the wild. Tissue culture could possibly help rebuild it in the future!

[@adamk.7177]

As long as there is good enough genetic diversity -- it would probably be good to research how many genetic lines are needed for stability.

[@conormarie4310]

I love carnivorous plants. Also really looking forward to the virus removal via meristem TC. I hope to apply it to raspberries.

[@raybod1775]

Love plants, science, tech… love your channel.

[@Casey_Bass]

Awesome video, can't wait for the next one.

[@edneyhelenedossantos1875]

This is really cool content thank you for that, love it

[@DogPrinctz]

You’re such a cool plant nerd!!!! Great videos

[@MikeLeady]

Thank you so much!

[@aviationcompilation455]

Awesome video!

[@rysrys5213]

Thanks 😊

[@laureneckert]

Yes!!! So cool!

[@hotrodAndy]

If you're a fellow enthusiast and lack an orbital shaker table, you can use a bed-slinger 3D printer like the Ender 3 to achieve the same effect. By writing some G-code, you can make the printer oscillate perfectly to continuously stir your sterilizing solution. Also you're videos are amazing thank you for all the knowledge! It's been about 10 years since I fired up my laminar flow hood

[@adamk.7177]

Makers are so funny lol, finding any excuse to use that 3D printer. Genius.

[@l.l.3073]

Awesome video please do one on staghorn ferns.

[@MikeLeady]

Thanks!

[@plantsinjars]

Thank you!! Glad you liked the vid

[@MikeLeady]

I appreciate you.

[@eini9430]

Can you please do a video on fruit plants like blueberry? And I watched one video where you mentioned all plants can be tissue cultured. So would be interested in knowing how to culture vegetables like onion, chives which don't really have stems but have a bulb.

[@antoniosanford4675]

I'm surprised that you or Franco have never been on the growing with fishes podcast. I'm sure they would love to have you.

[@egreen704]

nice video 🤩

[@jennypratt4293]

Love this, can you tissue culture Alocasia?

[@brianlegette3411]

Can you substitute Kinetin for BAP in your multiplication media protocol?

[@t-rexbros8794]

Can you link the forceps holder you have in the video?

[@bagusa.nugroho8335]

can you do TC on Agave Titanota?

[@rebekahstahl1018]

I would love to see tissue culture of seeds for hard to germinate plants ❤

[@retdeb4401]

Can we use kinetin instead of 6-Bap? If we use it, how many mg per liter should we use?

[@driqbal316]

If you do end up with contamination and the plants in the agar medium look perfectly healthy, do you still reject and throw the sample?

[@CriticoolHit]

The number 1 thing I learned here is I need a new stir plate. Mine CANNOT get a full liter (or more maybe) of water ripping and running like that without the bar flying off.

[@Salman-Q]

I guess I am one of the lucky few that got to discover your chanel in it's early stages 😍😄 P.s I still can't get over the fact that you hurt yourself with the scalpel by accident 🥺

[@daniellepine8521]

Hi in the propagation média, did you think i could replace the hormone for kinetin? If yes how much for 1 liter of média

[@zregn3620]

Hey! I have a question about tc. Is it possible to create a verigated plant in tc? Like take the non verigated version and use hormones to get it to verigate? I have been wondering this for a while, and can't really find anything online that answers this directly.

[@plantsinjars]

You need to start with a variegated mother plant to have a chance of getting variegated plants from tc

[@daniellepine8521]

Hi , did you use some ppm in some média for multiplication or rooting média ?

[@plantsinjars]

Nope

[@Omegawerewolfx]

Can you put pieces of flytraps with pieces of crocodile and make hybrids?

[@inxsgoat]

First comment! 😊 lovely vid

[@snillocekim]

Hey i like your channel, but i know nothing about tissue culture at all. why would creating tissue cultures of plants be useful? Is it to replicate or clone a specific plant in a sterile environment? Please elaborate!

[@Scooby-Snacks]

At 9:50 did you take that out of a refrigerator? Do you have content on storing TC in refrigeration? If not can you do that?

[@plantsinjars]

Nope! That was just a shelf kept at ambient room temp. Some people store tc in the fridge to slow down growth if they are trying to preserve genetics in vitro

[@Scooby-Snacks]

@@plantsinjars Cool, thanks for articulating. It would be interesting if you have the ability to dive in depth into preservation with refrigeration. Not sure if that's something you're interested in ever covering. I'm sure there's a lot to it. Thanks so much for making these awesome videos

[@nextagro]

the surface sterilization shown this video has nothing to do with the internal bacteria and fungi diseases of the plants.

[@apextroll]

I tell vegans that all the time: "All plants have feelings".

[@Robert-cd2ht]

I sense an evil plot to take over the world.

[@Marxnchill]

🐸😢

[@johnhockett8003]

Who is this girl

[@monkey_man70-1]

I hate these dumb poachers. What value do these plants have if they are sold at any hardware store? Theres only one natural environment for them in the US and these awful people just go in and take them like it's nothing.

[@Gts2pro]

Lab safety , tie your hair back in a pony tail please