Pour Plate Technique for Colony Counting_A Complete Procedure (Microbiology)

[@irfanullahkhan07]

Removing the media from the water & pouring into the plate the bottle neck should be flamed in order to avoid contamination.

[@praptipatel496]

Thank you ma'am 😊

[@MicroChemsExperiments]

You are welcome

[@suganyasuganya-ce6tu]

Excellent 👍

[@MicroChemsExperiments]

Thank you

[@pagsolinganjamielyndc.2]

Could we use this method to test the viability of probiotics in ice cream?

[@MaggsLingam]

Why do you turn the plate upside down?

[@paruljoshi2659]

to collect water of condensation on the lid.

[@MicroChemsExperiments]

Exactly

[@sabasiddiqui1492]

Please suggest me which media is suitable for fecal coliform test by pour plate method. And how we can prepare this media?

[@MicroChemsExperiments]

M-FC agar.

[@Muhammadahsan-cr8zh]

Mam is VRBA media in this vidio is Autoclave? because VRBA is not autoclave

[@MicroChemsExperiments]

Watch the video again. I just talked about general procedure media preparation. I said, Either you have to boil or you need to autoclave.

[@Muhammadahsan-cr8zh]

@@MicroChemsExperiments Now its clear

[@alejandropm6017]

Can I use this method with a complex sample (like soil or bee pollen) to first culture and count the colonies with serial dilutions and then use the cultures from the serial dilutions to isolate the different resulting colonies to create pure cultures?

[@MicroChemsExperiments]

Yes. You can

[@alejandropm6017]

​@@MicroChemsExperiments thanks, colonies will grow inside the agar or in the surface?

[@MicroChemsExperiments]

Inside the agar media

[@uyirgene]

@@alejandropm6017 you will get the colonies on the surface and sub-surface of the agar media.

[@group3project]

Does we want to add extra agar agar to vrba media during preperatiin ,can we boil the media in boiling water

[@MicroChemsExperiments]

You should add cold water in the media and then boil it.

[@SharonOwino-o5f]

Why is the media placed after the sample I thought its media then sample

[@ZoyyaKhalid]

What formula will we use to calculate CFU/g by this method? What will be the dilution factor?

[@sougata_rajak]

Maam , can we use this method for Nutrient agar ?

[@MicroChemsExperiments]

Yes. You can

[@venkybunny1554]

what about colony forming units please explain

[@MicroChemsExperiments]

Please take help from Google database

[@slsblizdhr6192]

what kind of agar base you're using in this tutorial?

[@thazinphyu1325]

For milk and Milk product and some product, we can use Violet Red Bile Agar

[@MicroChemsExperiments]

VRBL Agar

[@nurulizzah6248]

I want to ask. Stomacher bag are use or not in this experiment ?

[@MicroChemsExperiments]

We didn't use stomacher bag. Because no need of use in this technique.

[@nurulizzah6248]

@@MicroChemsExperiments seriously . Because our lab manual there is written has a stomach bag

[@MicroChemsExperiments]

@@nurulizzah6248 Generally, tomacher bag is used for sample preparation. But in this video, I didn't show any sample preparation. I just explained the procedure of pour plate method. That's why I didn't use stomacher bag in this video.

[@MicroChemsExperiments]

@Kylen Cannon Please don't discuss about any irrelevant topic here. Thanks for your coperation.

[@MicroChemsExperiments]

@Devin Jamari Please don't discuss about any irrelevant topic here. Thanks for your coperation.

[@rohitrs2403]

TQ

[@MicroChemsExperiments]

You are welcome

[@Zahid-h1t]

very bad background music

[@MicroChemsExperiments]

Sorry for that.

[@ezshanisiddarth5598]

Your from mam

[@MicroChemsExperiments]

Thanks

[@ayleen9580]

everything is good but y fake accent