Pour Plate Technique for Colony Counting_A Complete Procedure (Microbiology)

  Рет қаралды 104,841

MicroChem's Experiments

MicroChem's Experiments

Күн бұрын

Пікірлер: 43
@irfanullahkhan07
@irfanullahkhan07 25 күн бұрын
Removing the media from the water & pouring into the plate the bottle neck should be flamed in order to avoid contamination.
@group3project
@group3project Ай бұрын
Does we want to add extra agar agar to vrba media during preperatiin ,can we boil the media in boiling water
@MicroChemsExperiments
@MicroChemsExperiments Ай бұрын
You should add cold water in the media and then boil it.
@suganyasuganya-ce6tu
@suganyasuganya-ce6tu Жыл бұрын
Excellent 👍
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Thank you
@Muhammadahsan-cr8zh
@Muhammadahsan-cr8zh Жыл бұрын
Mam is VRBA media in this vidio is Autoclave? because VRBA is not autoclave
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Watch the video again. I just talked about general procedure media preparation. I said, Either you have to boil or you need to autoclave.
@Muhammadahsan-cr8zh
@Muhammadahsan-cr8zh Жыл бұрын
@@MicroChemsExperiments Now its clear
@sougata_rajak
@sougata_rajak Жыл бұрын
Maam , can we use this method for Nutrient agar ?
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Yes. You can
@praptipatel496
@praptipatel496 2 жыл бұрын
Thank you ma'am 😊
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
You are welcome
@alejandropm6017
@alejandropm6017 3 жыл бұрын
Can I use this method with a complex sample (like soil or bee pollen) to first culture and count the colonies with serial dilutions and then use the cultures from the serial dilutions to isolate the different resulting colonies to create pure cultures?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Yes. You can
@alejandropm6017
@alejandropm6017 3 жыл бұрын
​@@MicroChemsExperiments thanks, colonies will grow inside the agar or in the surface?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
Inside the agar media
@uyirgene
@uyirgene 3 ай бұрын
@@alejandropm6017 you will get the colonies on the surface and sub-surface of the agar media.
@SharonOwino-o5f
@SharonOwino-o5f 15 күн бұрын
Why is the media placed after the sample I thought its media then sample
@sabasiddiqui1492
@sabasiddiqui1492 2 жыл бұрын
Please suggest me which media is suitable for fecal coliform test by pour plate method. And how we can prepare this media?
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
M-FC agar.
@MaggsLingam
@MaggsLingam 2 жыл бұрын
Why do you turn the plate upside down?
@paruljoshi2659
@paruljoshi2659 2 жыл бұрын
to collect water of condensation on the lid.
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Exactly
@ZoyyaKhalid
@ZoyyaKhalid Жыл бұрын
What formula will we use to calculate CFU/g by this method? What will be the dilution factor?
@pagsolinganjamielyndc.2
@pagsolinganjamielyndc.2 10 ай бұрын
Could we use this method to test the viability of probiotics in ice cream?
@venkybunny1554
@venkybunny1554 2 жыл бұрын
what about colony forming units please explain
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Please take help from Google database
@nurulizzah6248
@nurulizzah6248 3 жыл бұрын
I want to ask. Stomacher bag are use or not in this experiment ?
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
We didn't use stomacher bag. Because no need of use in this technique.
@nurulizzah6248
@nurulizzah6248 3 жыл бұрын
@@MicroChemsExperiments seriously . Because our lab manual there is written has a stomach bag
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@@nurulizzah6248 Generally, tomacher bag is used for sample preparation. But in this video, I didn't show any sample preparation. I just explained the procedure of pour plate method. That's why I didn't use stomacher bag in this video.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@Kylen Cannon Please don't discuss about any irrelevant topic here. Thanks for your coperation.
@MicroChemsExperiments
@MicroChemsExperiments 3 жыл бұрын
@Devin Jamari Please don't discuss about any irrelevant topic here. Thanks for your coperation.
@rohitrs2403
@rohitrs2403 Жыл бұрын
TQ
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
You are welcome
@slsblizdhr6192
@slsblizdhr6192 2 жыл бұрын
what kind of agar base you're using in this tutorial?
@thazinphyu1325
@thazinphyu1325 2 жыл бұрын
For milk and Milk product and some product, we can use Violet Red Bile Agar
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
VRBL Agar
@ezshanisiddarth5598
@ezshanisiddarth5598 2 жыл бұрын
Your from mam
@MicroChemsExperiments
@MicroChemsExperiments 2 жыл бұрын
Thanks
@ayleen9580
@ayleen9580 11 ай бұрын
everything is good but y fake accent
@Zahid-h1t
@Zahid-h1t Жыл бұрын
very bad background music
@MicroChemsExperiments
@MicroChemsExperiments Жыл бұрын
Sorry for that.
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