Removing the media from the water & pouring into the plate the bottle neck should be flamed in order to avoid contamination.
@group3projectАй бұрын
Does we want to add extra agar agar to vrba media during preperatiin ,can we boil the media in boiling water
@MicroChemsExperimentsАй бұрын
You should add cold water in the media and then boil it.
@suganyasuganya-ce6tu Жыл бұрын
Excellent 👍
@MicroChemsExperiments Жыл бұрын
Thank you
@Muhammadahsan-cr8zh Жыл бұрын
Mam is VRBA media in this vidio is Autoclave? because VRBA is not autoclave
@MicroChemsExperiments Жыл бұрын
Watch the video again. I just talked about general procedure media preparation. I said, Either you have to boil or you need to autoclave.
@Muhammadahsan-cr8zh Жыл бұрын
@@MicroChemsExperiments Now its clear
@sougata_rajak Жыл бұрын
Maam , can we use this method for Nutrient agar ?
@MicroChemsExperiments Жыл бұрын
Yes. You can
@praptipatel4962 жыл бұрын
Thank you ma'am 😊
@MicroChemsExperiments2 жыл бұрын
You are welcome
@alejandropm60173 жыл бұрын
Can I use this method with a complex sample (like soil or bee pollen) to first culture and count the colonies with serial dilutions and then use the cultures from the serial dilutions to isolate the different resulting colonies to create pure cultures?
@MicroChemsExperiments3 жыл бұрын
Yes. You can
@alejandropm60173 жыл бұрын
@@MicroChemsExperiments thanks, colonies will grow inside the agar or in the surface?
@MicroChemsExperiments3 жыл бұрын
Inside the agar media
@uyirgene3 ай бұрын
@@alejandropm6017 you will get the colonies on the surface and sub-surface of the agar media.
@SharonOwino-o5f15 күн бұрын
Why is the media placed after the sample I thought its media then sample
@sabasiddiqui14922 жыл бұрын
Please suggest me which media is suitable for fecal coliform test by pour plate method. And how we can prepare this media?
@MicroChemsExperiments2 жыл бұрын
M-FC agar.
@MaggsLingam2 жыл бұрын
Why do you turn the plate upside down?
@paruljoshi26592 жыл бұрын
to collect water of condensation on the lid.
@MicroChemsExperiments2 жыл бұрын
Exactly
@ZoyyaKhalid Жыл бұрын
What formula will we use to calculate CFU/g by this method? What will be the dilution factor?
@pagsolinganjamielyndc.210 ай бұрын
Could we use this method to test the viability of probiotics in ice cream?
@venkybunny15542 жыл бұрын
what about colony forming units please explain
@MicroChemsExperiments2 жыл бұрын
Please take help from Google database
@nurulizzah62483 жыл бұрын
I want to ask. Stomacher bag are use or not in this experiment ?
@MicroChemsExperiments3 жыл бұрын
We didn't use stomacher bag. Because no need of use in this technique.
@nurulizzah62483 жыл бұрын
@@MicroChemsExperiments seriously . Because our lab manual there is written has a stomach bag
@MicroChemsExperiments3 жыл бұрын
@@nurulizzah6248 Generally, tomacher bag is used for sample preparation. But in this video, I didn't show any sample preparation. I just explained the procedure of pour plate method. That's why I didn't use stomacher bag in this video.
@MicroChemsExperiments3 жыл бұрын
@Kylen Cannon Please don't discuss about any irrelevant topic here. Thanks for your coperation.
@MicroChemsExperiments3 жыл бұрын
@Devin Jamari Please don't discuss about any irrelevant topic here. Thanks for your coperation.
@rohitrs2403 Жыл бұрын
TQ
@MicroChemsExperiments Жыл бұрын
You are welcome
@slsblizdhr61922 жыл бұрын
what kind of agar base you're using in this tutorial?
@thazinphyu13252 жыл бұрын
For milk and Milk product and some product, we can use Violet Red Bile Agar