Preparation of nutrient agar plates l How to make agar plate

In microbiology, it is very important to know how to make nutrient agar plates for routine microbiological work. Nutrient agar plates are prepared to grow microorganisms, especially bacteria.
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Hi, I Am Rahul B Rathod, Welcome to Our You Tube Channel RBR Life Science
About this video-
friends, In this video tutorial we are going to learn How to make nutrient agar medium plates and preparation of nutrient agar in petri plates for microbiology laboratory work thank you so much ....
Queries Solved:
1. How to make agar medium plates
2. How to sterilize media using autoclave
3. How to pour media into Petri plates
4. How to store agar plates in refrigerator
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#rbrlifescience

[@modoumangan9851]

Awesome, very clear and easy to understand

[@rbrlifescience]

Most welcome, also please suggest any topic or concepts i should make such videos

[@modoumangan9851]

@@rbrlifescience I will appreciate it if you make a video about putting bacteria broth into microtiter plate to measure their growth curve and optical density

[@TechsScience]

I tried this Experiment but got no reasult Also my solution looks whitish

[@rbrlifescience]

After autoclaving solution will be clear

[@TechsScience]

@@rbrlifescience but the agar solution is yellowish In general My solution remains pale yellow even after autoclave & yes I am taking an edible agar (used to make jelly) is it OK?

[@rbrlifescience]

Once agar in plate solidifies then it remains slightly yellowish. Nothing to worry about.

[@TechsScience]

@@rbrlifescience it's not about the color the problem is I am not getting bacteria culture even after 7days

[@vlogwithsanu377]

Thanku 🥰🥰

[@rbrlifescience]

Most welcome

[@Vjsnd]

This is great for beginners in the lab like myself :) Loved it!!! Thank you

[@rbrlifescience]

Glad to hear that ... stay tuned to watch such more videos

[@rachealpalmer9941]

Can I keep it for 48 hrs before refrigeration

[@rbrlifescience]

Yes off course

[@lovelyfeespanola7931]

Why do some nutrient gar need to add sodium for prep?

[@chemist291]

Is it necessary for antibacterial activity

[@sindhujan5241]

The best explanation out there. you are awesome 💯❤ ❤

[@rbrlifescience]

Thank you Dear

[@kolavinodha1242]

Sir can u make the vedio regarding the Isolation of fungi nd bacteria from spoiled fruits nd vegetables sir please

[@rbrlifescience]

The process is more or less similar as explained in the video. You can use your sample instead of soil

[@a110100]

Transfer a piece of the fresh fruit onto agar. Hopefully they grow separately and you can keep transferring away from the contamination.

[@sudiptakapri8624]

1.After making the nutrient broth we should check the the pH . 2. Before pouring the plates conical flask neck should be heated for contamination safety

[@rbrlifescience]

Yes, correct

[@dakavlogs9680]

Casi no entendí pero las imágenes ayudaron mucho para comprender y me siento mas seguro para hacer mi examen de esto

[@rbrlifescience]

English please

[@kael3808]

hi! what can we do if we’ll just sterilize the agar using pressure cooker? (we don’t have autoclave)

[@rbrlifescience]

Yes it will sterilize the agar

[@santoshbhavi8516]

Thank you very much for this informative video.

[@rbrlifescience]

Most welcome sir

[@joyamedu5680]

After preparing the Agar solution, can i use it to culture bacteria within 48 hrs without putting it in d refrigerator?

[@rbrlifescience]

Yes you can use plates after 24 hrs of incubation

[@upeducationtec9064]

thank you very much. well understood

[@UyenTran-ut5sy]

sir, why unused sterile medium should be then stored in the fridge rather than at room temperature condition?

[@rbrlifescience]

The moisture from the agar medium will evaporate, and plates will become dry. For microbial growth to occur we need water in the agar plate. Also if we keep the petri plates outside then there will be chances of microbial contamination.

[@All_in_1_YouTube_channel]

Hello sir

[@rbrlifescience]

Hi

[@divya821]

Sir make more how to reporting culture sensitivity and biochemical reaction

[@rbrlifescience]

Ok i will try

[@nishitachitkara4623]

But why we use autoclaving or incubator

[@rbrlifescience]

We use autoclave to setrilize and dissolve the media. After sterilization we pour the media in petri plate and after agar in plate solidifies we need to keep plates in incubator for drying for 24 hrs -48 hrs. This incubation is also check any contamination while pouring.

[@soniyarajkumari9419]

How do we know that we need 4.6gm of NA in 200ml of H2O

[@rbrlifescience]

On NA bottle, it is mentioned that for making 1000 ml of NA you need to take 28 grams of NA powder from bottle and suspend it in 1000 ml distilled water. It's just calculation 28 g for 1000ml 2.8g for 100 ml 4.6g for 200 ml Have a great day 😊

[@Talhanaqvi12]

But he didnt use sand for bacteria ??

[@rbrlifescience]

its animation for understanding

[@Pharmacygirl-struggles]

Because he's using nutrient agar powder.

[@Welcometoherbalworld]

Well explained

[@winwinkhaing468]

Why you convert plates? Is it really need or not ? Please explain kindly me?

[@rbrlifescience]

After pouring agar media in the plates once agar media in the plate solidify (usually after 1-2 hrs) then it should be kept in inverted position. Now two things are there, lid and plate with Media. If we invert the plate then due to weight of media in plate kept on lid there no air will enter inside the plate. If we keep the plate as it is after pouring then there are chances that air will enter inside and plate will be contaminated. This is the reason that all solid agar plates are always kept in inverted position in order to avoid entry of air inside the plate. Also plates are not contaminated while handling in inverted position.

[@gardeningonly]

Which is the good online platform to buy nutrient agar for india?

[@rbrlifescience]

It's himedia labs

[@gardeningonly]

@@rbrlifescience thank you i already checked there but they don't have 100gm I am just learning from your videos and blog so just want to try with small amount.

[@rbrlifescience]

You can purchase from Amazon you will get 100 gm

[@ManiMani-qj8rb]

Petri art video panuga

[@RealKing213]

From where we can buy nutrition agar and can grow orchid seed

[@rbrlifescience]

You can buy the nutrient agar from various companies like Hi media, thermo scientific, Merck etc. Few are also available on Amazon

[@soniyamourya8351]

Sir really this video use for me👍

[@rbrlifescience]

Welcome Soniya ji ...You can also suggest topics and I will try to make videos on it 🙏keep it up

[@HattoriHanzo62]

just a small and respectful comment: the correct measure unit to indicate "grams" is "g" and not "gm"

[@rbrlifescience]

Thank you for suggestion. I accept and I will definitely try not to write this gm for gram unit . Apology

[@HattoriHanzo62]

@@rbrlifescience no need to apologize, 🙂everybody can make small imperfection: the quality of the information is great and I just wanted to help to be more precise

[@rbrlifescience]

@@HattoriHanzo62 👍👍

[@zesseljhontaguibolos6621]

whathappen do them after 24 hrs, 42 hrs and 72 hrs?

[@rbrlifescience]

As the time passes the water in the agar gets evaporated if we keep plate in the incubator. A time comes when the agar falls from the lid after 5 to 6 days

[@divya821]

Sir make for culture sensitivity blood urine and so on

[@rbrlifescience]

ok i will try

[@somisettyharshitha7821]

Sir make isolation and identification of probiotic bacteria and yeast

[@rbrlifescience]

i will try

[@ligayan5143]

sir how do u compute for the powder agar to be used?

[@rbrlifescience]

If you are using ready made nutrient agar bottle then there is no need for computation. If you are preparing by addition of individual components then agar percentage is 1.5% For making 100 ml of NA take 1.5 g of agar powder For making 1000 ml of NA take 15 g of agar powder For exact composition read our blog post rbrlifescience.com/nutrient-agar-media-composition-and-uses/

[@Welcometoherbalworld]

Your videos are very helpful. Thank you sir, plz make more video of lab experiment

[@rbrlifescience]

Sure, please suggest me topics or concepts on which I should make videos

[@WaleedArif-i6m]

well explained

[@rbrlifescience]

Thank you So much and Stay Tuned

[@lvs8850]

very good video

[@rbrlifescience]

Thank you

[@Wrcher]

Thank you

[@rbrlifescience]

You're welcome

[@poleenterprise7676]

Thanks 🙏

[@rbrlifescience]

You’re welcome 😊

[@رضااحمد-ه2ت]

Thank you ❤

[@rbrlifescience]

Welcome

[@chkumaryadav657]

Nutrient Agar 37grams per 1liter so potatto Dextro Broth 24 Grams per 1 liter

[@rbrlifescience]

Whatever media you are preparing there is information available on Media bottle itself. Generally there is information given for how much media you should add to make 1 litre solution of that media .

[@chkumaryadav657]

@@rbrlifescience ok sir good information

[@manand287]

Thanku so much for u r hlep of my studies..god bless u

[@rbrlifescience]

Most welcome Sir

[@ruchika_899]

Tysm ❤

[@rbrlifescience]

Most welcome and stay tuned for such more videos

[@fansarulsk4415]

Blood agar macconkey agar video upload karo

[@rbrlifescience]

I got your request.. and I definitely going to make the video on blood agar and macconkey agar soon.👍

[@anassabduabdu9798]

Well done doctor

[@rbrlifescience]

Thank you and stay tuned to watch such more videos

[@rbrlifescience]

I am just Master of Zoology.

[@bernadettegaby]

For 23 gm of nutrient agar powder, add 1 L of distilled water. Is it right?

[@rbrlifescience]

yes correct, whatever media you prepare there is information available on bottle itself. Take 23 gm of nutrient agar powder add it in 1000 ml distilled water

[@hak8278]

28 mg for 1L👍

[@rbrlifescience]

It will depend upon which company media you are using. In this video i have added 28 grams of nutrient agar in 1 litre of distilled water. Instructions are always written on media bottle itself

[@arielbowmanoquinn7065]

I cracked up when he kept showing the petri dish pouring. 🤣 Classic.

[@rbrlifescience]

Thank you that you liked it

[@garageland_]

Change this accent please

[@rbrlifescience]

Ok thank you for suggestion

[@viratkohli7137]

Thanks sir

[@prabhadabahde1905]

Sir humko 17 g Lena hai kaise weigh kare

[@rbrlifescience]

First take a weighing boat or dish and keep it on digital weighing balance and tare the weight of dish to 0 . Now open the nutrient agar powder bottle and add the Nutrient agar powder in dish till the weight on weighing balance shows 17 gram.

[@prabhadabahde1905]

@@rbrlifescience thank you sir

[@rbrlifescience]

@@prabhadabahde1905 most welcome

[@rulakakatipandey9554]

Nice and stepwise and easily understanding to all....thank u sir

[@rbrlifescience]

Thank you for complement..... Stay tuned for such more videos

[@niloofarjadidi6030]

hi, what is the percentage of the ingredients of nutrient agar media?

[@rbrlifescience]

Nutrient agar media has different components such as Peptone, Beef Extract, Sodium Chloride, and Agar. For exact composition percentage please read our blog post rbrlifescience.com/nutrient-agar-media-composition-and-uses/

[@sawairagul251]

Well explained 🥰

[@rbrlifescience]

Most welcome..And stay tuned