Prokaryotic Transcription INITIATION - How do sigma factor & RNA polymerase initiate transcription?

References/Resources: / the_crux
The process of the transcription Initiation in prokaryotes. I explain the structural features of sigma factor 70, RNA polymerase (core- and Holo-enzyme) and the promoter DNA configuration and transitions. The main stages of the initiation process discussed are Closed Complex formation, Open Complex formation (with Transcription Bubble), Ternary Complex formation, and DNA scrunching.
Jump to your favorite section:
00:00 Introduction
01:20 Sigma factor structure
02:15 RNA polymerase structure
03:28 RNA polymerase holoenzyme
05:08 Closed Complex
07:39 Open Complex
09:40 Ternary Complex
10:30 DNA scrunching
Learn about prokaryotic promoter structure and organization: • Prokaryotic Transcript...
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[@okpolihenry983]

YOU ARE VERY GOOD AND DETAILED . THIS IS FOR GRADUATE STUDENTS NOT THE USUALL SIMPLISTIC ONES WE SEE ON KZbin

[@avonleastudy6535]

Exactly. it is so detailed and easy to comprehend. Thankyou the crux.

[@adrijabanerjee-ek6hw]

This is for masters and higher studies. Exactly what we need. Amazing Explaination

[@YourLocalLeo]

quick, clear and concise. great chapter organization, diagrams, everything is great.

[@gabrielatrevino9785]

love these videos! seriously saving my grade in genetics!

[@user-ig6or1sm5q]

your videos are so clear and interesting! thank you so much!!!

[@sumaib1]

well prepared and well delivered. I have to appreciate your drawing skills. Thank you.

[@user-fs1wr5ho9m]

You literally save my biochemistry! Thank You!!!

[@sudhavani4306]

Best vlog on transcription initiation

[@Audriavbruna]

SO HELPFUL MY GOODNESS!! THANK YOU!

[@mdshanewazhossan1894]

Excellent video brother.

[@claudiamorse]

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[@okpolihenry983]

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[@santoshhuded9688]

Nice explanation 👌

[@tanvirahmed617]

sir you r extremely helpful and so detailed . watching you from bangladesh .

[@Sunshine-hc3lt]

Thank you so much

[@Sweety__Tiwari]

Very helpful, thanks😇

[@ayeletchenchicheportiche4684]

wow!! so useful!!

[@ammarahmehak9986]

very nice video, keep it up

[@AspirantsForum]

Really love this marvellous content and teaching skills. You're lectures are like a beacon. Which textbooks do you recommend us to get such contact in print form?

[@janvi4589]

top content🔥🔥

[@theinnominatoidk3821]

Thank you

[@KseniaKlimova]

Thank you so much for such a fantastic and at the same time clear explanation! Much deeper than in an ordinary textbook) Which sources did you use?)

[@theCrux]

I am glad you found the content helpful :) I use relevant published articles and peer-reviewed discussions to assemble this content.

[@martineneillagateka5779]

May God bless you

[@islemben4845]

Thank you so much , and please what about sigma factor as a specificity factor

[@crabjoon]

a life save fr!!!

[@MohammedAhmed-pc9ys]

Great work Sir. Well prepared, highly organized lectures with an in-depth explanation. Can you continue your genetics series, please?

[@theCrux]

Thank you for the kind words and support. I do plan to continue the genetics series. Are you looking for a specific subject/topic in the genetics series?

[@MohammedAhmed-pc9ys]

Thank you Sir 😊. Regarding the suggestion for the genetics lectures, I guess, mutation types and consequences, as a start, will make perfect sense

[@theCrux]

Sure, I will put your suggestion on my list.

[@bioboi4438]

are the elements shaded in purple always there and part of the promoter or only in some certain circumstances?

[@lucidscience9554]

Thank you for this amazing video!!! I had a doubt here, is base flipping reversed once DNA scrunching proceeds, as in when the bases at -11 and -7 shift upstream of transcription bubble? And is base flipping exclusively observed in the -10 region only?

[@theCrux]

Great question! The current understanding is that base flipping is dynamic and reversible once the bubble gets too big - so there is a threshold length of RNA (made during scrunching) that triggers sigma domain 2 to disengage with the TATAAT sequence.

[@lucidscience9554]

@@theCrux Got it!!! Thank you so much!

[@harrypal8459]

The initiation by RNA polymerases requires the assembly of a large complex.outline the order of events that result in initiation ,and indicate the type of molecular interactions that are involved in transcription in prokaryotic? Pls reply this question answer in brief and pls plz plz sir reply quickly

[@pratibhasah6698]

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[@Biomeducated]

Made some promo for your channel on my FB page!

[@theCrux]

Thank you! I will also set up a Facebook page for my channel so that the information availability/update is more seamless :)

[@tanmoydas7095]

Sir thank you for the detailed explanation. You told that RNA pol directly binds to UPE for rRNA synthesis, and sigma factor does not bind to this particular UPE(-58,-37). So, does RNA pol for rRNA not need sigma factor?

[@theCrux]

Hello. I am not sure if I understand your question. In this video we are discussing mRNA synthesis. I don't think this video touches on rRNA. In prokaryotes, UPE is required for typical regulation mechanisms: Example, lac operon: see, kzbin.info/www/bejne/l5upfYWbpNqNqLc

[@tanmoydas7095]

@@theCrux Got it sir. I mis-understood. Thank you

[@NabanitaAB]

Please make video on Replication

[@theCrux]

DNA replication will be the next major series of videos. I am currently structuring and organizing the content. I hope to post those videos somewhere around March.

[@NabanitaAB]

@@theCrux Thanks a lot sir, glad to know that. It'll be very helpful to me just like your previous contents. Your contents are so much interesting and very very informative. I throughly go through all the videos , and those help me a lot. Keep going sir , you're one of the best. 👌🏻 👍🏻

[@ritumohapatra6960]

Ek min -10 and -35 coding strand par nahi hote?

[@anihysenaj3743]

great video! my doubt: you say ''when the number of nts is greater than 10 the transition from abortive tx to elongation takes place'' but when is it that this happens and why or is it casual?

[@theCrux]

Excellent question! 10 nts is sort of a "magic" number (just an average actually and not a strict rule) - when the RNA is synthesized, the exit channel is blocked by the sigma factor, so the enzyme tries to push it out but it fails often. During this failure, the RNA is released, which we call abortive transcript. But after a few tries, the sigma factor is kicked away and the exit channel is cleared out, which allows the RNA to move out and the transition into the elongation occurs. You can find more details in the DNA scrunching part of this video: kzbin.info/www/bejne/bZzJoph_n66tg7s

[@anihysenaj3743]

@@theCrux super clear! Thank you very much!

[@sammilton6593]

how dna unwinds during elongation in prokaryotes

[@theCrux]

Hello Sam! For transcription elongation in prokaryotes, please see this video - kzbin.info/www/bejne/bZzJoph_n66tg7s