Promoter Delation Assay/EMSA

[@chirayutrivedi7648]

Excellent explaination sir... Thank you 🙏

[@azadbharath7444]

Q 1:Option C:A&D is correct Whynot c ?: If repressor btwn -868&-432 deleted, expression of little much should be higher at -432 than -868.

[@earth2k66]

There is no surety if the region downstream -432 isn't a part of the repressor as well. The data you get from the graph shows the expression going below the control in the last two black bars. Deleting a repressor won't do much if the upstream enhancer is already removed. D statement can't be true, the majority of the binding site was present in -1210 and -868, the expression wouldn't have changed in the second bar as well.