Reverse Transcriptase PCR (RT PCR) | What are the applications of RT PCR ? | RT PCR protocol

This video talks about the principle behind RT PCR. Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).[1] It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Combined RT-PCR and qPCR are routinely used for analysis of gene expression and quantification of viral RNA in research and clinical settings.
#animated_biology #animated_biology_with_arpan

[@florenciachandrikahalim7664]

Sorry, I can't give a 'super thanks', but I would like to express my sincere thanks for your videos. I am able to understand a lot about biotechnology and then passed my exam. I'm a vet student from Indonesia. 🙏

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance.

[@SuperLinhi]

I have a question: when I have transcribed the mRNA into cDNA, do I perform a normal PCR with the cDNA? Can someone pls tell me if i'm right or wrong ? PCR steps 1) denaturation of the template DNA 2) use primer and poymerase 3) amplification RT-PCR: 1) isolation of RNA 2) transcribe with reverse primer and enzyme reverse transcriptase into cDNA 3) follow PCR steps

[@geekymedico.8099]

Amazing explanation ... thank u sir...🙏🙏🙏🤗🤗🤗🤗🤗🤗

[@mariafernandalealtamez9844]

Very good info! Do you have the references??

[@ikennafranklinokoro4252]

Great video, comparison of RT-PCR and PCR was a benefit

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@ritikyadav3014]

Hello sir, i want to go for research but i don't know how to find research institute for my topic of research can u help me that how can i search institute.

[@davidace7]

very good, detailed and concise explaination, thank you sir!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@divyasvlog4410]

@@animatedbiologywitharpan sure sir ... lov your explanation

[@alejandropikoulasplata5524]

Thank you so much for these very useful short tips!

[@animatedbiologywitharpan]

Thanks for your support. Please feel free to suggest more video topics in comments.

[@jagjyotkaur72]

Very well explained thanks !! The animations are really good

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience.

[@jagjyotkaur72]

@@animatedbiologywitharpan yes I did share it with my friends it is really helpful

[@kaushik6122]

Thank you so much sir.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@IronMan-dj1uw]

Hello Bro You have not included the further steps Like After making the hybrid rna-dna .The hybrid's rna is partially digest by Rnase H ,then DNA polymerase I forms a complimentary strand and nick is sealed by DNA ligase and Thus forms a cDNA

[@animatedbiologywitharpan]

Yes these are nitty grity details. It was a overview hence did not get into details

[@yokoshiko9427]

Great help on exam eve😇🤩

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@sangitamandal6146]

It was very helpful ❤thank you sir😊

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@hidayat.609]

awesome

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@priyankachauhan4976]

I can't thank u enough for this... just keep going 🙏🙏🙏

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@alperentur1543]

very nice channel thanks from turkey arpan

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience. By the way I love 💘❤ Turkey and the amazing food. I still remember my visit to Istanbul.

[@animatedbiologywitharpan]

I just love baklava and kebabs

[@Xahidd____]

Can anybody tell me if reverse transcriptase posses 5'-3' exonuclease activity then why aren't Hexamers degraded instead they are incorporated 😢😢

[@user-so7ng7bf8x]

Thanks, good content, should give you thumb up.

[@animatedbiologywitharpan]

Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.

[@nilufertek3177]

you saved my todays prentation sir, thank you very much!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@shubhamrana41999]

sir is rt pcr is same technique used in covid rest or it is different .

[@animatedbiologywitharpan]

That RT pcr is real time PCR also qPCR . One step of that is reverse transcription which makes cDNA from RNA . After that qPCR is done from the cDNA.

[@umapriscilla6243]

Thank u sir

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@sanajiya4462]

Yawrrr such a super duper lecture thank you so much🥹✌️

[@animatedbiologywitharpan]

Pcr playlist kzbin.info/aero/PLKtiwIJ8Q7rpWfwFl7dpbamj09MQ7ynPT&si=sCuhziSFd8j-dSPn

[@Yousha999]

Hi thank You for the well explained video can you explain microRNAs

[@animatedbiologywitharpan]

Ya there would be series on all the regulatory RNAs soon

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@dhamotharan6745]

Sir can u explain about multiplex pcr

[@animatedbiologywitharpan]

Sure it would be uploaded soon

[@n0skies]

thank you

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@AdityaRaj-zk9eh]

thanks alot

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@anusreesaha4624]

Please upload a video on allele specific PCR.

[@animatedbiologywitharpan]

Thanks for suggesting

[@__jm__6762]

Dont we use a specific primer in RT pcr with those 2 primers?

[@animatedbiologywitharpan]

Generally no. In Reverse transcriptase PCR all mRNA are converted to cDNA . Ideally there should not be any bias. During qRT PCR( quantitative real time PCR ) we use specific primers.

[@__jm__6762]

@@animatedbiologywitharpan thank you so much sir

[@animatedbiologywitharpan]

Please follow my Facebook page for notes

[@animatedbiologywitharpan]

Facebook page : facebook.com/pages/?category=your_pages&ref=bookmarks

[@__jm__6762]

@@animatedbiologywitharpan thank you so much sir

[@myeverydaylife20]

hello dear. The RT-PCR consists in 1 cicle only ??

[@animatedbiologywitharpan]

For reverse transcription protocol yes

[@animatedbiologywitharpan]

For q RT PCR ( quantitative real time PCR ) se the following video kzbin.info/www/bejne/laGwdKqIerZlaKs

[@myeverydaylife20]

@@animatedbiologywitharpan Thank u so!!!!

[@jennymacparry1855]

What if we don't use hexamer primers???

[@animatedbiologywitharpan]

Alternatively you can use oligo dt

[@prachimate2238]

can u plz explain tma and nasba

[@animatedbiologywitharpan]

Can you tell me the full forms

[@sabinabegum419]

Inverse pcr k upar ek banayega plz

[@animatedbiologywitharpan]

kzbin.info/www/bejne/rKWrqIKNgrWHgpI

[@rebeccajohnson3402]

Where does the reverse transcriptase itself come from?

[@animatedbiologywitharpan]

You provide it in the reaction mixture, reverse transcrtase for this purpose is syntheticly made.

[@rebeccajohnson3402]

@@animatedbiologywitharpan In vivo I mean. Does it exist within our bodies or does the viral RNA code for it as well.

[@Apoorva4102]

@@rebeccajohnson3402 it's isolated from AMV virus :)

[@MolecularMedicine327]

Please refer the references i need to write my notes thanks in advance

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@user-sy5td7ft2u]

References please🙏

[@animatedbiologywitharpan]

www.thermofisher.com/in/en/home/life-science/pcr/reverse-transcription.html

[@Jesusandbible]

if you claim to be knowledgeable ...... is this a real quotation or not? “Quantitative PCR is an oxymoron.” Kary Mullis, inventor of PCR. As it it is then almost all the stats we have about Covid are bunk.