Reverse Transcriptase PCR (RT PCR) | What are the applications of RT PCR ? | RT PCR protocol

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Thank you so much for these very useful short tips!

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Niceee information 😊

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Great video, comparison of RT-PCR and PCR was a benefit

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[@IronMan-dj1uw]

Hello Bro You have not included the further steps Like After making the hybrid rna-dna .The hybrid's rna is partially digest by Rnase H ,then DNA polymerase I forms a complimentary strand and nick is sealed by DNA ligase and Thus forms a cDNA

[@animatedbiologywitharpan]

Yes these are nitty grity details. It was a overview hence did not get into details

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Very well explained thanks !! The animations are really good

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[@davidace7]

very good, detailed and concise explaination, thank you sir!

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@@animatedbiologywitharpan sure sir ... lov your explanation

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It was very helpful ❤thank you sir😊

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awesome

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I can't thank u enough for this... just keep going 🙏🙏🙏

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[@geekymedico.8099]

Amazing explanation ... thank u sir...🙏🙏🙏🤗🤗🤗🤗🤗🤗

[@sanjeevyadav1976]

Sir is this technique used for hiv detection

[@animatedbiologywitharpan]

can be used

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very nice channel thanks from turkey arpan

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Great help on exam eve😇🤩

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Thanks, good content, should give you thumb up.

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[@mariafernandalealtamez9844]

Very good info! Do you have the references??

[@__jm__6762]

Dont we use a specific primer in RT pcr with those 2 primers?

[@animatedbiologywitharpan]

Generally no. In Reverse transcriptase PCR all mRNA are converted to cDNA . Ideally there should not be any bias. During qRT PCR( quantitative real time PCR ) we use specific primers.

[@__jm__6762]

@@animatedbiologywitharpan thank you so much sir

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@@animatedbiologywitharpan thank you so much sir

[@anusreesaha4624]

Please upload a video on allele specific PCR.

[@animatedbiologywitharpan]

Thanks for suggesting

[@dhamotharan6745]

Sir can u explain about multiplex pcr

[@animatedbiologywitharpan]

Sure it would be uploaded soon

[@sanajiya4462]

Yawrrr such a super duper lecture thank you so much🥹✌️

[@animatedbiologywitharpan]

Pcr playlist kzbin.info/aero/PLKtiwIJ8Q7rpWfwFl7dpbamj09MQ7ynPT&si=sCuhziSFd8j-dSPn

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Thank u sir

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thank you

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[@SuperLinhi]

I have a question: when I have transcribed the mRNA into cDNA, do I perform a normal PCR with the cDNA? Can someone pls tell me if i'm right or wrong ? PCR steps 1) denaturation of the template DNA 2) use primer and poymerase 3) amplification RT-PCR: 1) isolation of RNA 2) transcribe with reverse primer and enzyme reverse transcriptase into cDNA 3) follow PCR steps

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can u plz explain tma and nasba

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Can you tell me the full forms

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you saved my todays prentation sir, thank you very much!

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[@myeverydaylife20]

hello dear. The RT-PCR consists in 1 cicle only ??

[@animatedbiologywitharpan]

For reverse transcription protocol yes

[@animatedbiologywitharpan]

For q RT PCR ( quantitative real time PCR ) se the following video kzbin.info/www/bejne/laGwdKqIerZlaKs

[@myeverydaylife20]

@@animatedbiologywitharpan Thank u so!!!!

[@jennymacparry1855]

What if we don't use hexamer primers???

[@animatedbiologywitharpan]

Alternatively you can use oligo dt

[@thisslightlysweetlife3402]

Where does the reverse transcriptase itself come from?

[@animatedbiologywitharpan]

You provide it in the reaction mixture, reverse transcrtase for this purpose is syntheticly made.

[@thisslightlysweetlife3402]

@@animatedbiologywitharpan In vivo I mean. Does it exist within our bodies or does the viral RNA code for it as well.

[@Apoorva4102]

@@thisslightlysweetlife3402 it's isolated from AMV virus :)

[@KaushikRachna.2024]

Thank you so much sir.

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[@shubhamrana41999]

sir is rt pcr is same technique used in covid rest or it is different .

[@animatedbiologywitharpan]

That RT pcr is real time PCR also qPCR . One step of that is reverse transcription which makes cDNA from RNA . After that qPCR is done from the cDNA.

[@sabinabegum419]

Inverse pcr k upar ek banayega plz

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kzbin.info/www/bejne/rKWrqIKNgrWHgpI

[@Yousha999]

Hi thank You for the well explained video can you explain microRNAs

[@animatedbiologywitharpan]

Ya there would be series on all the regulatory RNAs soon

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[@AdityaRaj-zk9eh]

thanks alot

[@animatedbiologywitharpan]

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[@Xahidd____]

Can anybody tell me if reverse transcriptase posses 5'-3' exonuclease activity then why aren't Hexamers degraded instead they are incorporated 😢😢

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Hello sir, i want to go for research but i don't know how to find research institute for my topic of research can u help me that how can i search institute.

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References please🙏

[@animatedbiologywitharpan]

www.thermofisher.com/in/en/home/life-science/pcr/reverse-transcription.html

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Please refer the references i need to write my notes thanks in advance

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[@Jesusandbible]

if you claim to be knowledgeable ...... is this a real quotation or not? “Quantitative PCR is an oxymoron.” Kary Mullis, inventor of PCR. As it it is then almost all the stats we have about Covid are bunk.

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