RNA splicing part 2 (self splicing)

[@flutiegirl4193]

I'm using your videos to help with bioinformatic analysis of a sequence from maize for my senior undergraduate thesis. Very helpful! You are a good teacher. Thank you.

[@shuvronilchakraborty8163]

What are you doing now?

[@erginsahin6490]

Thank you for this good, educative video regarding RNA self-splicing. This is indeed a brief explanation of a self-splicing event taken place in self-splicing group II introns. In the case of a self-splicing group I introns, exogenous guanine is used as a cofactor for the first transesterification event which results in break up of 5' end of the intron (Guanine is transferred to this end) from the upstream exon. As a result of the second transesterification event, the intron is released and the exons are ligated to each other. Lariat formation is not seen in group I introns.

[@rizwanullah6467]

Tell me name this book plz

[@bisma0564]

Shoumu sir z superduprr but yeh he told this thing by mistake...😊

[@asmakar6151]

You explain very difficult things very easily.and it is perfect

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@Kinderverse1234]

Increase the playback speed and watch, it will save lots of ur time.

[@AGMP9]

Thank you

[@ashu_the_stylish]

Time is taken for understanding..... If you are genius then go and read your book within a short time...!!

[@scottamon8908]

Sir you are very good at explaining these concepts. You are much better then my professor.

[@shomusbiologyofficial]

+Scott Amon thank you. Glad you liked my lectures. Please subscribe

[@luisar3968]

Thank you you are my favorite youtuber that helps me with my school

[@shalinipatel9584]

He corrected it ..Excellent! Simply superb!!

[@priyatripathi1802]

Can't thank you enough sir !! Your videos are just amazing. You are a saviour sir ! God bless you and thanks. 😊

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@johntindell9591]

Thank you so much. You are literally the best at teaching this.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sanjibkalita403]

you are really celebrity for biology student. thank u very much

[@ashu_the_stylish]

Sir I love your classes...... You re so hard working person, I have seen in your videos sir..... Your videos are helpful to any biology student...💓

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@ashu_the_stylish]

@@shomusbiologyofficial you deserve that sir... 💓

[@fakhsol2771]

Thank you You work hard I enjoy your describtion of self splicing It is so useful 🌹🌹

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@sourabhrajput293]

Bhaiya bhot jeeoge aap yarr😂😂😂. Thanks alott

[@kannanpg14]

Sir am msc microbiology student. Ur videos are very helpful. Thanks sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@tabayallum3828]

Thank you so much sir due this lock down period or sllabus was incomplete but ur lecture is very much helpful...

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@sp7351]

aren't the blue rectangles exons at 18:42 ? Would appreciate your confirmation, thanks and great lectures! =D

[@sp7351]

nevermind, just saw 20:43...

[@manaswinibal6594]

Thank you sir ur video are really helping 🥺 ,its lots more easier to grab the concept,plz make video on biotechnology plzzzz 🥺🥺🥺🥺

[@shomusbiologyofficial]

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[@bikkeychettri1333]

Thank you sir, referring your videos for my NET preparation in this lockdown period.💐

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@divyaa6884]

I just love your videos❤❤🤗🤗 & for you ,,molecular biology is easier than before,, for me...😊😊❤❤

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@divyaa6884]

@@shomusbiologyofficial ❤❤

[@asreekhan7372]

But he is missing something

[@ChandrachurMukherjee96]

The rna is requiring the splicing due to these introns only... so if it helps in removing itself... does it really have advantage Most of the time when the exons get mutated introns take over the function.. This is the only advantage

[@anitasaha4597]

Thank you !!! Helped me a lot for my entrance.

[@shomusbiologyofficial]

You're welcome

[@umersaleem2590]

you have mentioned 5:47 that the splicing signals present on the intron are like AGGU at both 5 end as well as 3 end of intron which is contradictory to the what the literature mentions about the structure cum signal for splicing. The splice donor site includes an almost invariant sequence GU at the 5' end of the intron, within a larger, less highly conserved region. The splice acceptor site at the 3' end of the intron terminates the intron with an almost invariant AG sequence. Upstream (5'-ward) from the AG there is a region high in pyrimidines (C and U), or polypyrimidine tract. please explain.

[@ashajaluthariya1511]

for me u r god of knowlage...💜

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@mrinusand]

you're lectures are highly helpful! Thanks!

[@bettymariya7578]

👍super explanation 🙏

[@shomusbiologyofficial]

Thank you

[@PriyaSingh-nk7mt]

Very good video Pls make another video for lariate formation mechanism of splicing

[@shomusbiologyofficial]

Will. Make it soon

[@samsonteklehaimanot5665]

Hello Suman Bhattacharjee, You really helped me to get ready for my exam .I really appreciates your time. Do you have DNA repair and replication, recombination and Protein synthesis video as well??

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check his channel. it's awesome! there is DNA REPAIR AND REPLICAITON I BELIEVE

[@richasrivastava5867]

It really helped me a lot. Thanks for your videos. Take Care

[@wakaderahul1095]

Thanks sir your lectures helpful to preparation of exam.....

[@m.sc.partiibiotechnologysi1546]

good lectures sir... it helps a lot !

[@shomusbiologyofficial]

+MSc Part 1 thank you.

[@manju8109]

Explained very well. Thank you

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@manju8109]

@@shomusbiologyofficial Yes definitely, your lectures are very helpful in understanding difficult concepts.....Thanks again. Keep it up :)

[@neethugopal2351]

Thank you. It's very helpful for my study

[@shomusbiologyofficial]

You're welcome

[@kanikachoudhary7697]

Very helpful video ....thnku so much sir

[@shomusbiologyofficial]

You're welcome

[@anaraserikbek92]

you're truly gifted! are you involved in any research or doing your own reseach?!! you should apply to MIT!!!

[@vidhyasatha]

Thank you for this video it's really helpful for my exams:)

[@thakurprathvisingh4048]

Great explanation 👌

[@rasmisahoo5522]

. really grateful to u Sir🙏😊Sir is the ligase enzyme is essential for joining of two exon or not?

[@ARASHIop77]

excellent description sir but you did not mention how two exons join together? i mean is there any enzyme involved which ligates the two exons together?

[@shubhambhardwaj5946]

this two step reaction is also called transestrification reaction because phosphate is react with alcohol

[@mfbvlog]

Iam addict to your lecture 🔥

[@sushantgupta8969]

Thanks sir for making it more easy

[@shomusbiologyofficial]

+Sushant Gupta glad it helped

[@soudaminidas2999]

Very helpful vedio ... Thank you so much sir ....

[@shomusbiologyofficial]

You're welcome

[@ujjainimukherjee4328]

You are the best!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@pri6458]

Sir please make a video on homing and retrohoming🙏

[@shilpasatapathy6665]

You're just excellent

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@ranaasadmehmood776]

However, very good explanation 👏👏👏

[@Raptor9323]

13:50 they are exon 1 and 2 right ? you told intron ?

[@Raptor9323]

thanks to 20:48 i was confused the whole time

[@prakritijha4050]

By mistake he said

[@nagmakhan5106]

Very nicely explained

[@shomusbiologyofficial]

+nagma khan thank you.

[@ChandrachurMukherjee96]

Actually you forgot to show the 5' carbon in the ribose sugar structure( instead put the oxygen there)

[@rubinaamanat3311]

Good job👏

[@shomusbiologyofficial]

You're welcome

[@neeturaghav1931]

Superb explanation

[@shomusbiologyofficial]

Thank you

[@alearner3404]

Pranam Sir! Sir kindly give me the notes of Splicing or tell me where I can find the notes of splicing? It'll be helpful for me . Thanks a lot❤

[@vijaykumardas1379]

Why -OH group attacks only G-G sequence?

[@VinodKumar-oi5fw]

awesome ,.,, wonder full job , I really hats off u

[@ramachandrabehera2347]

By stop

[@AGMP9]

Thank you sir 😊 👍

[@shomusbiologyofficial]

You're welcome

[@ranaasadmehmood776]

Oh my god Aap ki intron wali mistake ny guma K rkh dia tha mujy..... 😁

[@jawadsayed4534]

Hye Why only adenine is present in branch point cant there be any other base and secondly if RNA can do splice itself than why it sometimes tends towards spliciosome mediated splicing ?

[@jawadsayed4534]

@@rajniyadav6085 yes that is but why only adenine

[@vaishalithukral9129]

Why you didnt talk about group I and group II introns.

[@rizwanullah6467]

Tell the book name from which you teach this topic plz

[@shomusbiologyofficial]

Don't recall now

[@rizwanullah6467]

@@shomusbiologyofficial why sir! I need this plz

[@-_-ok9550]

Thankyou so much.

[@shomusbiologyofficial]

You're welcome

[@hananiyakarada7519]

Thanks u so much sir ....

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@madhumitachhatria2910]

Sir at the last where you are indicating the cleavage actions that are not introns..that are exons which will cleave 1st due to nucleophile attack please rectify the mistake

[@Avra_b]

Excellent. Thank you :)

[@pattiaditinaidu1802]

Thank you sir

[@shomusbiologyofficial]

You're welcome

[@shanuverma9932]

Wonderful

[@shomusbiologyofficial]

You're welcome

[@shanuverma9932]

@@shomusbiologyofficial sir can you please do a video on mechanism of gene expression in yeast .

[@prabirsbiology8220]

Sir 🙏 Why dna ligase is not use here? Ans plz

[@khannagma7553]

Because it is a RNA

[@sidhunayak3562]

Thank u

[@shomusbiologyofficial]

You're welcome

[@sharathkumarism]

excellent lecture

[@durugesh6651]

Good but very much slow ..

[@sudarshanreddy5115]

Tq sir 🙏🏿

[@shomusbiologyofficial]

You're welcome

[@dineshahir1291]

I think you do one mistake you write exon position intron.😭 But you are genius mistake showed.👍

[@nehaverma1440]

Thank you so much sir..

[@tanyasingh1934]

Thankful

[@shomusbiologyofficial]

You're welcome

[@ritikasingh8809]

Thanks!!

[@shomusbiologyofficial]

You're welcome

[@nothingbutbiology3163]

There are some wrong in this video. Where he wrote intron 1 and intron 2 this will be exon 1 and exon 2

[@shomusbiologyofficial]

Yes. Sorry. I need to delete this video

[@pawanbakthiyal2759]

Thanks sir

[@shomusbiologyofficial]

You're welcome

[@rabbitlover8288]

Sir would u provide me the notes and video s for preparing net exam .. n tell me the fee of it ?

[@shomusbiologyofficial]

You can buy our csir net study material pack for Rs. 10300 only

[@rabbitlover8288]

@@shomusbiologyofficial sir apne kisi video m is se related btaya h apni to bta dijiye kuki tda detail to chaiye hga na hume b

[@pramitlahiri1978]

2:02-bookmark sequence

[@alwinaanam1231]

very helpful..

[@pratikshapatil5652]

Sir I don't have clear this concept

[@mbinbkk]

bro you called exons introns the whole time

[@Isaiaswolf66]

He corrected himself in the middle

[@abhishekpati2457]

Intron and Exon identification is incorrect

[@zohashakeel101]

Good but slow..

[@shreesumanas6027]

Dear Sir, Thank you so much...It would be great if u help me by uploading the mechanism of DNAzymes

[@aparnadey4914]

Sir it's exon 1 and 2. Not intron

[@ayyuen]

OMG