Spore Syringe to Agar - The Agar Files

Today's class I'll be going over 3 different methods to germinate spores from a syringe onto agar. Thank you as always for the love and support.
Inoculation Loop Video - • Cheap and Easy DIY Ino...
0:10 Intro and Prep
2:17 Shake up your Spore Syringe
4:55 1-2 Drops on Agar
5:23 Inoculation Loop on Agar
6:28 Sterile Swab on Agar
7:28 Cleanup and Storing your Agar
8:30 7 Days after inoculating
9:20 10 Days after inoculating
10:16 Outro
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[@AyyYaYYaY]

"Break this JAWN Apart" Philly is Really in the house.

[@themyceliumnetwork]

here is another tip: with the syringe cap on tight pull the plunger a short distance and quickly let it go, it will create a shockwave & disperse the spore clumps.

[@chipmeister111]

Nice, thanks

[@Enochulate88]

Here's a tip: throw soil on top of your sub when your done fruiting it to make a indoor compost bin. Lots of fun

[@AllFameKings]

@@Enochulate88 after harvest? or?

[@zachm1452]

As a medic I've found that if you pull back on the syringe before you push the solution out you have much more control over how much comes out during that first push.

[@Sasha8pancakes]

But you risk letting contams enter the syringe probably

[@Sasha8pancakes]

Adding on to my previous reply, if done in a still air box it would probably be kinda safe

[@jessicahay9305]

You can place a just slightly damp alcohol wipe over the end first. As an addict in recovery I can confirm that it's much easier if you pull back first

[@damonwilks8799]

Risking pulling in contaminates in the air? Why risk it

[@timebombcatnip]

I purge that first push into a shot glass, its all dead spores anyway you just cooked um.

[@landen702]

Another tip for controlling drops is just squeezing the sides rather than pushing the plunger. Thanks for what you do for us PGT!

[@AbdelkaderBoudih]

That awesome, you just use it as a pipette. Genius.

[@unparalleled3039]

Syringes are hard plastic.

[@fungdark8270]

That can introduce air back into the syringe

[@jeffsmith8065]

@@fungdark8270 you do it without the syringe

[@greatestytcommentator]

Yeah Bro.. You are a Genius..

[@jakemccarrey]

If the clumps are super coagulated, I’ve secured the syringe to a 12v Dewalt saws-all cutting blade on the rig and shook the ever loving bajesus out of it. That will DEFINITELY get the clumps apart. Sterilize accordingly afterwards. 👍🏽

[@voteazblue]

Sensei, thank you, thank you, thank you! I'm starting my journey in mycology and microdosing to help my grandson who suffers from epilepsy, Crohn's, and depression. Of all the DIY videos I've watched, yours call to me. I've inoculated agar plates and made brown rice mini-jars (pint). Wish me well!

[@gizzardgizzard3583]

You sound like an amazing grandmother! Hope all is going well :)

[@PuppetMasterdaath144]

haha nuts to see an older woman make drugs

[@DeltaNovum]

Much love to you and your grandson. Hope you're both doing well.

[@GingeRenee]

your such an amazing woman and grandmother. I hope your seeing amazing results from the plant medicine to help your grandson. I truly believe mother nature gave us everything we need. Blessings to you and your grandson.

[@Aces314]

Hey, I’ve recently had my first two flushes ever and want to thank you man, I’ve got knowledge from you that I’ll hold forever. Thanks alot

[@gloriadw1]

Newbie here and your content is great!! I’m learning sooo much. Thank you for explaining everything extensively. After watching other channels and finally hearing you explain things to where I understand it - you hear many “ohhhhhhhs” coming out of me. Your channel is quickly a favorite. Thanks for all the great content!

[@AbraxasTheSorcerer]

3 videos in one weekend! You the man 🤙🏼, we appreciate all your efforts. I've been growing cannabis for 10 years and now with the help from you, and members of the mycology community on KZbin and reddit, I've been slowly buying all my supplies over the past few weeks while I study the craft before I start. This will probably never get read but thanks brother ✌🏼🍄 EDIT: 8 months later and I’ve grown pounds of TATs 😂🤙🏽 (side note) I also just had my very first contamination 🤙🏽

[@SquashDaBeef]

Dude just go for it! Learn on the job :)

[@monkeyboy4311]

Best. Hobby. Ever. 🤙💚

[@michaelcoleman5922]

I could say the exact same thing as Psychonaut. Been growing herb for years and thanks to Teacher and a few other, my fruiting room is ready and I'm waiting for my spores to come in. Can't wait.

[@kibby3164]

F KZbin and Reddit. If you want info on cultivating, foraging, or anything that has to do with mycology. Then go to The Shroomery.

[@michaelknichel2528]

@@michaelcoleman5922 Where did you get your spores?

[@Humboldtcounty707]

Man, I wish this was posted yesterday at this time! I just did the squeeze one drop method. Should have done the streak method. Oh well, thanks PGT!

[@sel.lodjalosalvador159]

same here

[@yeagsntoastable]

I have way more than a couple drops

[@kevinfromcali7062]

Hello PGT, really just wanted to express my gratitude for all your hard work in the videos that you're doing, I'm a newbie at this only about 3 weeks into it but I'm so excited about it, I don't know why I'm so drawn to it, I think you're doing such a wonderful job and I really just wanted to convey that, thank you so much.

[@LeonPhythian69]

The confidence wrapping with the parafilm shows pure practice. ❤

[@jacobpie3692]

Love learning from someone so knowledgeable, and I absolutely love your video style. You put a lot of work into this channel, good freaking job my man

[@eementexi3062]

Having a shitty day... came home took some food open youtube and new video from PTG! Now my day is a lot better :)

[@gizzardgizzard3583]

Idk if he ever says so in the video, but definitely store your agar dishes/cups upside down before and after inoculating to cut down on contam caused by condensation mushlove

[@Yokoname]

i remember learning this in my freshman year of highschool in biology 😂

[@gizzardgizzard3583]

@@Yokoname that's way cool! I unfortunately had to find this out the hard way I lost all my first agar cups to contam bc they weren't upside down

[@Paul4Krista20]

Thank you for this info! I didn’t partake in any science fairs since I was usually hiding under classroom portables high as a 🦒 a$$ 😅

[@hunnitkaydj]

Keep them right side up while they are innoculating?

[@gizzardgizzard3583]

@@hunnitkaydj i keep em upside down still while innoculating the spores still make enough contact to make some mycelium

[@davidhalldurham]

I'm just a beginner and this video was extremely helpful. Thank you.

[@god910]

@ 5:08 you mention the first depression of the syringe causes way too much to come out. I was hoping you were gonna say something. I work in the medical field and our saline flushes do the same thing. To resolve this, simply pull back on the syringe, it breaks the stopper free and then smoothly moves forward. ;-) But be careful not to pull it backwards out of the body, just a tiny bit to free it up, twisting sometimes helps too.

[@VeronicaCastro-ru8nm]

Most amazing coincidence this month! Love your videos and literally this weekend got plates to do SS to agar, glad I waited you read my mind!

[@kimberlymarie9512]

I'm glad you are feeling better 💜

[@devethic]

Thanks for the content! Not trying to sound unappreciative with my last comment - it's just helpful to get an idea of the process/order of events when working with an SAB because it can confusing trying to parse together when to go out of the box then back in to flame sterilize the needle etc without disrupting the still air. Been watching lots of different videos to try to piece it together, this one helps a lot!

[@AaronAlso]

I suggest leaving out the shot glass and picking up a 5ml graduated cylinder. It helps me to avoid using too much solution. 1ml will innoc dozens of plates. Also, a pipette gives much more control over the single drop. [5ml grad cylinder w/ glass pipette $6.99 @ Amazon] Same equipment can be used to transfer LC to plates or slants.

[@ChrisMosner]

I like the sound of this method. How much liquid do you drop onto each plate?

[@AaronAlso]

@@ChrisMosner Literally 1 drop or less is all that will be needed.

[@mmpatriot2170]

So good. You make info that seems incomprehensible simple. I’m stoked to start working with agar. 👍

[@PhillyGoldenTeacher]

I'm glad I could help. Good luck starting your journey with agar! 😊

[@irenedelvalleprietoguia8102]

Wow! Vegan ham candy! This video makes me so happy! Thank you!

[@karinchristensen9809]

Many Thank yous dear Jack! Looking forward to seeing where the peitre dish goes next!! 😉

[@PhillyGoldenTeacher]

😄

[@karinchristensen9809]

I can see some white stuff in one of my bags. What now?? Thanks you!

[@greatestytcommentator]

@@karinchristensen9809 Well? What Happened Next?

[@michaelgrouse7850]

Please make a video about how you made your still air box. I'm getting into the hobby (collecting my tools and supplies now) but have yet to see a good how-to on a good still air box and yours seems luxurious as you have room for a camera inside. ;)

[@toddwmac]

Thanks a ton PGT. Thanks in a big part to you, I just received my 1st mycology research kits today. Your lessons have been invaluable in my learning and planning . Now I just need to pour those 200 agar plates. :) Thank you.

[@Deverydoo]

Well darn. I could have used this video yesterday. Searched a bit and this pops up today. I used the direct inject method onto the agar. Shaking up all the spores was the hard part. Didn't think to add a slight touch of air to help the solution shake better. Figure the cotton swab method will work best.

[@electronicsrus8546]

Hi my name is Lance. I am a new grower and I just watched one of your videos and I like how simple your videos are been watching a lot of other videos trying to learn the mycology. Just wanted to say thanks and I’m going to watch more of your videos

[@PhillyGoldenTeacher]

Thank you lance, I wish you the best on your journey! 😁🍄

[@mrkwa-385]

Always nice to see a good education video from u mate!

[@BlueNukez]

your whole channel is a blessing!

[@tylerbrandon9592]

Love your videos bro! Just wanted to add my own cheap and easy inoculation loop I use. I just got a black head popper from the dollar store 😂😂😂, all stainless steel and can be put in a PC to sterilize if necessary

[@markgarry7866]

Thank you bro, just getting started with some lions mane, King Oyster, and others, I picked up some innoc loops and sterile swabs for a few bucks on Amazon, will be swabbing in a couple of days, thanks for all your great vids. After agar grows out, will transfer to agar, and l.c. and see how things progress, then onto my masters mix, and grain/ verm mixes to see which varieties grow best on each medium...thanks again.Pgt

[@kerimacgill7489]

Yet another great video Teacher! You show so many great ways to get shrooms started that it's nearly impossible to fail! Thanks again!

[@MeadsyYT]

literally what I was looking for, just ordered spore syringes yesterday and was wondering if I could start messing with agar, thanks!!!

[@sk8urbike935]

Wow when you were tapping the spore syringe on your finger it exactly matched the music in the video

[@PhillyGoldenTeacher]

😄

[@neoasura]

Thanks for this, I prefer using Agar cups, but once I'm done growing for a season, I put everything away. All I have left is spore prints in storage. Going from spores to agar allows me to skip a step.

[@jermainelightfoot7060]

Great vid , I was wondering how to put spores to agar 💯💪🏾 you answered all my questions

[@anthonyfarley4990]

Homeade ingenuity brother, been following your ways for a minute with great luck forsure (: lol keep up the good work!

[@TAC0FACE]

Only had one successful grow 2 summers back. This summer i had bought all this stuff, going for some oats jars and pf tek jars... I used agar and thought I had some good plates, they looked good. I cut up the agar, plop it into the jars, they colonized quick. Then i opened them to mix w coco coir and peeee yew smelled rancid... Slightly yellowish. Had to dump it. Gonna go at it again. I pressure cook everything lol! I don't have a good, use a SAB.. something got in it. Well spring is here, time to try again

[@injegomontoya370]

Great videos! I need to get better at spotting contamination. I thought that plate was good. I'm gonna check more videos , you probably have one about spotting contamination already.

[@MrCasperV.]

I knew I forgot something . Well I'm learning . Thank you for sharing your knowledge . It's literally gold ✨️ .

[@ANJH1106]

Mush love been following you since high school love your work thanks so much for your inspiration!

[@Akashic_Monk]

thank you great video

[@kels884]

No matter what I do, I get massive condensation in my agar plates. Any tips? Thanks pgt & glad to have you back.

[@PhillyGoldenTeacher]

You can stack your plates right after pouring, the heat will rise through the plates and remove condensation from the lower plates. the best method to eliminate condensation completely is to leave your plates in front of the flowhood for 2-3 days after pouring. the laminar flow slowly makes its way through the plates and dries up condensation.

[@tyo6896]

I gave mine a quick shake, and was able to get droplets to condense, to see spires growing, but now I gotta leave it tilted or upside down till the water dries up.

[@atomcatz69]

Hell yeah, thanks PGT!

[@dustinstoudt7753]

Dude I was literally looking for a video on this last night. Woke up to you uploading one lol

[@lemonberries]

Thanks for the info! Im gonna try this tomorrow

[@riverdreamwalker3693]

Well I tried the spore syringe ti agar well got a squirter on both plates so now I think the loop will be my next try

[@jalishaboatman1468]

I’m so new to this. I have a few questions. 1. What do I do with the leftover syringe? 2. after the inoculation process is complete, what comes next and then after that? I need help. 😅

[@FlojHabs]

music taste goes hard homie

[@PhillyGoldenTeacher]

😄🍄

[@johns9990]

Pls keep the great videos coming! You are the best teacher on here for shroomies!

[@oscarm.j1340]

His got lots of good Stuff like, Psilocybin, Psychedelics, also have Shrooms, Spores Syringe, Micro-dose, Dmt, Lsd, Valium, Modafinil, XTC, Xanax, Grow kits, Grains, 1P-LSD, AL-LAD and lots more. He could guide you through anything you need..

[@oscarm.j1340]

Feelgrabz,,,,,...

[@oscarm.j1340]

Reach him via his Instagram handle or Telegram channel....

[@GarconTheGuy]

This channel is so cool. Thank you for the informative content!

[@daysensouth7325]

How can you tell the difference between mycelium and mold? They look so similar except the mold wasn't as thick looking

[@tyo6896]

I've been using zip lock sandwich bags to store my plates in, after micropore tape wrap or pararfilm. Agar requires a bit more of a sterile environment while working with. I've had bad luck unless I'm very very careful.

[@frankblackington2477]

You rock I appreciate your info! Do you have any videos of what to look out for as far as what contamination on an agar plate looks like once you start getting growth ?

[@RedneckSavant]

idk if it was intentional, but you were pretty on time with the song when you were tapping the syringe!

[@th3word487]

Great info. Thank you.

[@fns5757]

"if you dont shake it you probably just innoculated a bunch of water"

[@clayhicks7175]

You should make a video on choosing a microscope i have been considering purchasing one lately to aid my mycological hobbies

[@idkthatxool749]

I would also be very interested in this

[@chris-has-problems]

This dude is great 👍

[@BadddDoggg-id4po]

Actually, on that last plate you looked at it may not be mold on the bottom, that may be a monokaryon mycelium, the thicker stuff looks like dikaryon mycelium. The mono won't fruit if you try to grow it out.

[@alejandro_grande13]

This is awesome. I've been wondering what the point of the agar is (first time grower)

[@LuisTorres-bo2mz]

Love the jawn at 3:02 I’m from Philly so I caught it 😂😂

[@PhillyGoldenTeacher]

😄

[@patrickmcgovern676]

What I'm looking for is taking your inoculated spore agar and making a culture in a syringe from a slice. Is this possible and is there a video with this !!! Thank you Patrick McG. Rookie mycologist self taught journey. Please reply I would be very grateful. Have a wonderful day. 😊Mush Love !!

[@davideluciano7761]

What can you do with culture still in the syringe can u put it in the fridge

[@PhillyGoldenTeacher]

Yes you can 🙏🍄

[@AlphaTk]

Is it possible that spores doesn't need oxygen to grow? you have it in sealed petri dish & it make us very curious how to actually get that blue agar for growing mycelium. I think this the cleaner method, which i would like to attempt. Thank you for sharing this information with us.. had no idea if mushroom spores can grow in agar dish.

[@Daweedmon420]

Hello PGT! So I live in the tropics and the temps are higher and pretty much stay to a now more than 7 to 10 degree flucation. WIll Mycelium grow even at my temperatures 76/87, Thanks for the help

[@PhillyGoldenTeacher]

Yes it will grow 🙏

[@Hogbellylife]

Great video thanks for the info!

[@michaelkreisel3573]

I made 40 cups and had 100% failure 😥 they’re all slimy and they all stink 😡 I’m pretty certain I squeezed to much juice in my agar. I’ll try my luck at cloning and maybe some spore agar. Thanks PGT

[@TheGr8Herd]

For me it was less that the agar itself intimated me and more everything that might be neede d to use it successfully. You know, SABs,Flow hoods,etc. Now that I understand a bit more about sterilize procedure and how to make an affordable, effective SAB it doesn't seem so bad.

[@guardianofthesacredflame1649]

How did you get the spores in the syringe?

[@davist-mq5db]

Thanks alot for everything

[@streamingharsh]

Amazing 🤩🤩

[@jmichel70]

Thank you

[@edwardramos1726]

Great job.

[@nnguyen3482]

once the spores are on the agar, whats the best/ideal conditions for the agar? room temp in the dark ect.. going to give agar a go with all the info learned . thanks PGT

[@vectionnoitcev2836]

Room temp is fine, anywhere between 75 and 80 F is optimal.

[@nnguyen3482]

@@vectionnoitcev2836 thanks, how long in these conditions would one expect to see some growth?

[@vectionnoitcev2836]

@@nnguyen3482 Anywhere from 4 days to a week. It's been 4 days since I inoculated dishes and one is showing signs this morning. The timing of your question is perfect! Two dishes were done from a spore print and six with spore syringes, 2 dishes for each variety. The spore print wins the race so far. I put 4 little drips from the spore syringes on each dish just to be sure but no activity as of yet...

[@nnguyen3482]

@@vectionnoitcev2836 haha your timing is on point too. I'm going to try both ss/print later today. thanks for the help

[@courtneyb6154]

@@vectionnoitcev2836 Hi! Did your syringed spores ever pop?

[@pkgmsu]

Does PT come on here to answer questions? Why didn’t he eye loop the first dish on the left side? Where does one get those nice stainless racks for working in front of hepa?

[@donaldslayton2769]

Mechanical agitation for the spore syringe? Tape it to the "back" massager for a few minutes?

[@PhillyGoldenTeacher]

I don't see why that wouldn't work 😄

[@nickb3105]

Maybe a stupid question, but is there an advantage to putting it on agar as opposed to right in a grain bag?

[@PhillyGoldenTeacher]

Spore syringes are naturally dirty, and agar allows the ability to clean up the culture before introducing it to your grains

[@theguitarden6920]

Do you have any tips for identifying mycelium from anything else in a petri dish? Obviously orange or green is bad, but I have had petri dishes full of thick white fluffy stuff, which I imagine isnt mycelium but it is hard to know

[@davids11131113]

Fluffy white is mycelium

[@scottxd45]

Is there any need to put a needle on the syringe for anything done here. Could you not just drip it straight from the syringe?

[@PhillyGoldenTeacher]

the needle helps you get more control on squirting, but yes you can just use the syringe without the needle.

[@derrick_v]

7:25 scary smiley face in syringe

[@idkthatxool749]

Lol that’s awesome

[@dosisemmanouil]

After the spores have germinated you just get a piece of it to grain?

[@PhillyGoldenTeacher]

Ideally you want to make a few transfers of the mycelium to new dishes to clean up the culture. But yes when the spores germinate, they produce mycelium that can be transferred to grain jars 🙏🍄

[@dosisemmanouil]

@@PhillyGoldenTeacher Thank you🙏❤ much appreciated all the info and videos!! Greetings from Greece!

[@jeremytalbot8565]

So I definitely need to put my spores on agar first. I knew it had to be harder then just injecting spores. 🤣

[@RaceReady2A]

Yo good lookin. I appreciate the content you put out . I’m on my first time doing this and I’m hoping for the best because I’m a beginner and want results but I’m also an avid and experienced grower of many things so I understand failure for growth.. it Looks like we have the same Agar., mine is called Rapid Rhizo.. hopefully this works, I’ll watch your video on Agar to grain bag I’m pretty sure I seen one on your video uploads.

[@akashaspirit6150]

Does anybody know how long the agar cups can be stored before they go bad out of the fridge?

[@PhillyGoldenTeacher]

about a month at room temperature. you probably can still use them longer than that, but for being optimal and efficient its best to use them sooner rather than later.

[@whygamingwhy2404]

I'm curious, is there any reason to use specific recipes for agar instead of just doing water agar? Is there a reason we don't? Does it just not grow?

[@charlesmjohnston]

What did you do to sanitize, disinfect that shot glass ? Just hot soap and water, dried with a paper towel?

[@devethic]

Could you do another video like this but inside an SAB?

[@rustylimbo]

Why would one choose to create more opportunity for infection? IE. Squirt into shot glass, then to loop or swab then to agar? Seems to me the less transfers the better - drop from syringe to agar, done?

[@Crypt0Crossf1re]

[@robertpowser5624]

Where is best place to store plates after fully colonized? How long will plates be viable?

[@jeffreyhalverson2611]

Good Stuff! Thanks:)

[@PhillyGoldenTeacher]

😁🍄

[@riverdreamwalker3693]

Why just a couple drops would it be beneficial to make little drops all over?

[@philipclift7205]

Can you drop spores on the plate and spread them with a flamed loop and skip the shot glass step? Hybrid of your 3 techniques.

[@lewismclean6285]

What if you buy a spore syringe and you can't see any spores at all in there? Does that mean I've been scammed or is it possible there's spores in there somewhere? I've innoculated some agar just to check so will give that a week or so to see before kicking off lolz

[@elizabethr2908]

I’m new to mycology. Do I store the LC with the needle on or off the syringe after inoculating the agar? Do I recap the syringe? Love the video!

[@mariobass4202]

Name of the song please??? Thank you PGT for your videos I find it very helpful sensei keep kicking ass!!

[@seanyardley]

Hey pgt, Sorry if this question is answered in a different video but is there a reason your agar is blue? Is it just a different type of agar and is there any difference between the yellow agar I am used to seeing?

[@PhillyGoldenTeacher]

You can add food coloring to change the color! 😄

[@seanyardley]

@@PhillyGoldenTeacher thank you man 👍🏻

[@chicofromph33nix64]

When I do spore syringe to agar,I don't use a needle. I take the cap off and squeeze 2 drops onto agar