My New Favorite Liquid Culture Recipe for Growing Mushrooms

[@ZCRAIZED1]

After trying the original recipe with a 64th of peptone and LME and karo I just put together another 7 quarts of the suggested recipe for more nutrition. The first way shown in this video worked very well. I simply want growth for another round as fast as i can get it and plenty of nutrition to fill the jar with mycelium. The suggested recipe was 1G peptone, 1G LME and half tbsp of honey or corn syrup. I mixed the first quart that way and while stirring it i noticed it was definitely not completely clear. i lowered the LME to between.5 and .75 if a gram, not measuring it exactly just leaving the 1\4 teaspoon less than full and that solved the problem if the slight haze. Absolutely clear LC with that recipe. 1G peptone which is 1\4 teaspoon, .5 to .75G LME, light malt extract and half tbsp 7ml of Karo. I used karo only because that is what was there and honey seems to caramelize when i use it at 4% I have not tried it at lower concentrations, it might be perfectly fine. I wanted to list my findings for others who are trying to get completely clear LC but still wanting high nutrition. Thanks a million for all your help, especially the trick with adding 2 inches of water covering the jars in the bottom of the all American pressure cooker so I don't cause a haze after sterilization. It worked perfectly. I ran it between 12 and 13 psi after getting it to that pressure for 35 to 40 minutes and immediately vented. Perfect results. Thank you very much!

[@Biokemist-o3k]

If you use filtered air through a Luer loc filter disc you will see the mycelium grow in huge leaps and bounds. One in and one out and a 45 dollar aquarium air compressor. I say the 45 dollar one because you will want numerous jars going at one time.

[@ZCRAIZED1]

​@@Biokemist-o3k I have all of those parts right now. I use a blunt tip needle that goes all the way to the bottom of the jar so putting air in that should cause a lot of oxygen going through the liquid and to the bottom of the jar. Thanks for the idea, I am going to try this method.

[@Biokemist-o3k]

@@ZCRAIZED1 Excellent my friend! In Early 2000's I was part of a company in Kennet Square Pa. that had a culture collection that rivaled the National Type Culture Collections. I started cultivating medicinal and gourmet mushroom spawn that we supplied to large mushroom grow houses. With fresh air I had to double the amount of peptone or malt extract because the mycelium grew so fast. We ended up selling the company to Campbell's soup and I suppose it still exists. It was called Mushroom Science Laboratories. keep up the great work.

[@DARKW1ZARD616]

Hey man do you know how much (7ml or half Tbsp) of Karo is on grams

[@Biokemist-o3k]

@@DARKW1ZARD616 1/2 tablespoon is 7.39....milliliters so the answer would be yes to your exact question.

[@ProperPoliticsWithLociKittie]

Funny story...been watching your videos and ordered all the stuff in this one so that I can start growing my own liquid culture. I just went on etsy and randomly selected a liquid culture syringe to get me started. I didn't even look at who the seller was until I had sent the payment. Turns out it was you XD

[@SporenSprout]

That is funny! 😂 Thank you and good luck with your experiments! 🧫 🍄

[@agnesm.w.5937]

Spore n' Sprout is a great place to learn about fungi growing!🙂

[@Tephomab]

I stuck spores from a MSS into coconut water as an experiment and it started growing myc after 4 days.

[@Slothen]

i love the clearness and very fast growth of this recipe. thank you! however, most of my strains now start to make significant rafts. i first thought it was contam, but surely they are not all suddenly contaminated. it gathers up in big floaters on top that is very hard to break. have you experienced the same?

[@SporenSprout]

Yes I experience the same, You have to stir frequently to prevent the mycelium from doing that and becoming too thick. You can switch to honey LC if it becomes annoying. Or you can watch my blender video where I blend the LC: kzbin.info/www/bejne/gnjZcqhmqt1gjKssi=Or-S6wzPFjK0Y62v

[@Slothen]

@@SporenSprout i was thinking if they get a bit too much nutrition still? have a feeling it could be related. not feeling super comfortable with these rafts, and the additional step of blending i would also like to avoid. what do you reckon? i'm coming from honey LC and that's been working fine, but i feel the clarity of this method makes it easier to spot bacterial contam.

[@Grand-TJ-Bear]

I've been using this recipe for a out 6 months now, i use a little more peptone and malt extract but it looked like we get the same kind of results, as soon as you showed the ingredients I got excited, this is by far the best LC recipe I've used

[@SporenSprout]

It’s really a game changer! I love how clear it is and how fast the mycelium grows compared to just honey. Thanks for watching and Good luck !🍄

[@codyl.807]

Hands down the magic sauce

[@IrishEagIe]

​@@SporenSproutWhat's your process on growing indoor chicken of the woods?

[@adammichaels800]

@@IrishEagIe that’s my question as well. So tasty!

[@tedkravos7129]

I once stored it in cold cooler and it ruined my LC. It wouldnt grow anything anymore. Can I just keep it in a kitchen cupboard for a few months? Did that only take you 2 weeks to have that much mycelium from just 1 cc of LC injected in that jar?

[@armadillo9961]

Great video and thanks. Thats 1/64 tablespoon. A teaspoon is about 1/8 of a tablespoon. So 4x that small one you got there or 1/4 of the big one (the size of a nail) would be a teaspoon.

[@SporenSprout]

Why does the spoon say “1/64th TSP” right on it then? It would say TBS if it was 1/64th tablespoon. Either way it doesn’t matter, you can use more nutrients than that. The only reason I used such small amounts was because I had sediment but I found out it was from not using enough water in the pressure cooker. Make sure the water level is touching the bottom 2” of the jars while pressure cooking.

[@armadillo9961]

@@SporenSproutthe spoon is wrong :) Americans generally have a different understanding of teaspoons they are really tiny in the rest of the world. I like your content just wanted to point that out

[@fattymcbastard6536]

I love how this comment section is basically a huge list of FAQs *that are being answered!* Keep it up, man. You've earned another subscriber.

[@justniksmycosilva]

Thank you for this recipe. What do you think about using powdered corn syrup? Does mycelium have a preference of bone peptone over soy, or vice versa? Do you feel that this broth will work for pestilotiopsis microspora, initially anyway? We are excited to find out how this works with all of our different species. We have a number of projects going on both professionally and personally that this may be a nice addtion too. Thanks again for sharing it with is all!

[@SporenSprout]

I’d go with soy over bone. The bone peptone makes the mycelium grow on the surface too much and also smells bad. Yes I think it will work great for any microorganisms really. I have never heard of corn syrup powder but for me corn syrup is never strong enough. I like honey.

[@hoosierdaddy70]

once grown how can you store to preserve and how long will it last?

[@SporenSprout]

@@hoosierdaddy70 I store them on a shelf in a room that is 60F and they last 1-2 years perfectly fine like that.

[@BretCrandell]

First of all, thank you for sharing all your knowledge! If a person wanted to run an exhaust fan a few times an hour for short periods of time, do you have any recommendations as far as a timer that is that precise? Thank you again!!

[@Myco_N3rd]

I used this exact recipe. Used 5ML of a LC into 600ml and its been 6 days i can not beleive how muxh the myc has grown! It is blowing my mind

[@patrickmanasco8772]

I had good sterilizing in microwave for abt 5 min

[@hAckAbleMe]

Microwaves kill most of the benefitial nurtients, ya know.

[@MatviiLeonovych]

Hello! First of all - amazing video and results! I would use some advice if you don't mind: I'm using 8 grams of fructose in 200 ml of distilled water. Sterilized the solution and inoculated it, kept the jar wrapped in the aluminium foil at 24-26 Celsius. It's been 4 days now and the only thing that appeared in the solution is a very thin and short transparent (or white, it's hard too tell they are so tiny) strings of supposedly mycelium. Also the spores (they appear as very dark brown dust) are doubled in size. Is it going well and I should proceed with waiting or the result I have now (4 days) is not enough and I should just scrap it and start all over again? Thank you in advance!

[@SporenSprout]

It might be ok but spores are not very clean to begin with so it’s not adviced to inoculate with spores. You should germinate the spores on agar first and then transfer to LC media. You will need to check the liquid you made on agar to know for sure if it’s good

[@MatviiLeonovych]

@@SporenSprout gotcha, thanks for the answer!

[@ZeOnetruth]

Praise God for all of Eternity!!!❤️‍🔥👑🪽

[@gruntymunky4082]

ive had cultures last 3 years in a dark cool closet..........dont disturb the water wall inside and the mycelium will give off a digestion enzyme that sterilizes the water and essentially puts it in stasis.

[@ZCRAIZED1]

I didn't see this and as usual I mixed everything in hot water, the peptone took a small amount of agitation to get it mixed but all three ingredients were very easy to mix in hot water. LME and I'm using light Karo just because that's what is here. I have honey but at 4% it badly clouded and a lot of sediment. I'm guessing I caramelized it with too much heat and too long of a temperature.

[@basantaphukan7262]

Thanks for the valuable content. What amount of dextrose can be added instead of corn syrup?

[@SporenSprout]

1/2 tablespoon of dextrose

[@hungnamnguyen9003]

Hey amazing video!!! Quick question: does this recipe works with lion's mane mushrooms?

[@SporenSprout]

Yes it works for all species

[@hungnamnguyen9003]

@@SporenSprout can I replace malt extract with malt extract agar?

[@SporenSprout]

@@hungnamnguyen9003 no because it will become a solid instead of liquid due to the agar.

[@hungnamnguyen9003]

@@SporenSproutThank you so much for relying to me!! But is there a catch for this method? Is there a limited time you can do this? I also watched your video on grain to grain transfer. Is there a limited times you can do that also?

[@SporenSprout]

@@hungnamnguyen9003 you certainly can’t do it forever. After 1-2 years you will need to start again from spores which is pretty easy anyway.

[@fattymcbastard6536]

Thanks to these LC videos promoting this bacteriological peptone, Amazon is sold out. I decided to order the soy peptone instead. Given these results from something sooo diluted, we all need to promote this recipe so there's more peptone to go around as this mycological revolution ramps up 😉. Good to know at least that LC solutions can be successful over such a broad range of concentrations. I don't think I'll even bother measuring after watching this; just a couple of pinches of each to be sure I have at least this amount, but obviously waaay less than other recipes I've seen.

[@bradleyhawley7519]

Do I need a vent along with the self healing port?

[@SporenSprout]

Yes I use 2 layers of micropore tape.

[@Lucas_Beetles]

Hello, im interested in to trying your way, but the metal lid ever get rust over time? do you think its better to use plastic with ring in it?

[@SporenSprout]

Do not use a drill, use a hole punch like this one: amzn.to/3BzLr1r

[@cHemist4Higher]

The video shows you using 1/2 tsp but the description says 1/2 tbsp. You also way 1/2 tbsp for dextrose.

[@SporenSprout]

@@cHemist4Higher I already answered this many times in the comments, you can look at them to see my response.

[@pipephotos]

Once you got the mycelium grown and stirred you can store it for 12 months untouched or do you still stir it daily?

[@SporenSprout]

You can stir it once every 1-2 weeks at that point.

[@RipperYouTubeOfficial]

I got your spores in the mail super fast, thanks. I'm about to make these mixes today, and eventually use them to mass produce shrooms in my 2400sqft shop. Let's goooooo

[@SporenSprout]

Nothing better than that 🙌🏼 Good luck brother! 🍄

[@SporenSprout]

@@EricCheetoh How to Clone Enoki & Shiitake Mushrooms from the Grocery Store kzbin.info/www/bejne/e3K1f62EfaatnJI

[@HavokBWR]

What do you think about adding brewers yeast? Ive seen other recipies with this but im not sure what it does.

[@Giggle_Slayer]

Can you link your pressure cooker?

[@SporenSprout]

@@Giggle_Slayer This is the one I recommend: amzn.to/3AtAU7u

[@Giggle_Slayer]

@@SporenSprout I already run 2 of thoes at once. I was hoping the one you had in the video had a larger capacity?

[@devinoliver1376]

you know how G2G is like 3 generations then start a new batch? is it similar with liquid culture? or once you make the culture, you can keep going with it and not worry about generations

[@SporenSprout]

@@devinoliver1376 It’s similar.

[@Chrissiesmile]

Hi what an informative video. thanks, I am making mine to your recipe and need to know what temperature to store the jar at?

[@SporenSprout]

You can store them at 70-75 F. When mixing the ingredients you should start with room temperature water. The ingredients do not dissolve well in hot water. You can use 1/4 teaspoon (1 gram) of each powder as long as the water is not hot. It should dissolve perfectly in cold/room temp water. It also helps to reduce PSI from 15 to 13-14 to prevent opaque cloudy LC media. Good luck!

[@Chrissiesmile]

@@SporenSprout thank you for your detailed response

[@ZCRAIZED1]

I wish I would have seen this video last night. I only saw the short one and didn't catch the statement about watch the full video. I made 7 quarts of the lighter nutrient content. I'll be making this heavier recipe tonight if I can figure out what to do with my all American canner to get it to not make the mixture clouded. I'll definitely take your advice about running it at less than 15 PSI. Thanks for everything, cheers.

[@ZCRAIZED1]

I didn't see this and as usual I mixed everything in hot water, the peptone took a small amount of agitation to get it mixed but all three ingredients were very easy to mix in hot water. LME and I'm using light Karo just because that's what is here. I have honey but at 4% it badly clouded and a lot of sediment. I'm guessing I caramelized it with too much heat and too long of a temperature.

[@dirtydavesgarage8251]

does the water have to be distilled if its boiled first since its being sterilized in the PC?

[@SporenSprout]

@@dirtydavesgarage8251 No it doesn’t have to be distilled. Use a presto 23 quart pressure cooker and make sure the water level is touch the bottom of the jars.

[@CarlosCardenas-t5o]

Thank you brother! Peace and love☮️❤️🙏🏻

[@MrYusha]

LME is the best IMO! Clear and fast growth!

[@Poolshockguy]

the rubber stoppers and filters that come with jar lids, do yall leave those on when sterilizing in cooker

[@SporenSprout]

@@Poolshockguy Yes

[@Andy-pj6ek]

I've been using a recipe that uses about 1/2 tsp peptone and 1/4 tsp LME, but the same amount of corn syrup and water. You've found these much smaller amounts of peptone and LME to be superior to larger amounts like that?

[@SporenSprout]

Yes in my experience the smaller amounts work better. If you can fit more in without turning the solution cloudy than keep doing that. If there is any cloudiness the mycelium will be initially inhibited in my experience. I’m thinking a hot plate will allow you to dissolve more without it compromising clarity.

[@Andy-pj6ek]

@@SporenSprout Interesting. I'll definitely try this out.

[@thiagorossi3860]

Thank you for your videos! They are very educational. I am more familiar with your old videos. Trying to catch up now. I feel my knowledge is a bit rusty after over a year break from studying mycology. I had experimented little and due to health conditions I was forced to have a break and my LC spoiled meanwhile. I’m ready to restart but now all I have is 1 spore print and 5ml spores suspended on water. Unfortunately I am not able to get more. I have materials for grains on jars, bags, agar, LC, bulk substrate… What would you recommend I start with so I make the best of the little I have? For grains I have access to rye and popcorn by the way. I am interested in starting a good self sustaining cycle loop so I don’t waste the spores I have. Your help would be greatly appreciated. Thank you in advance!

[@X.L.B1]

Honestly before watching this, I thought to make this you’d use part of the end product, like making a spore print or putting some on to agar. Does this just multiply your initial 10ccs and that’s it or is there a bigger plus that I’m missing?

[@SporenSprout]

Spore -> agar -> LC Yes It propagates the mycelium.

[@ZepHoffman]

hi bro, can i use the spore syringe ,directly into the liquid culture ? thanks @@SporenSprout

[@SporenSprout]

@@ZepHoffman Yes you can but spores are not sterile so there is increased chances of contam. You’d have to definitely test the resulting liquid culture on agar plates. Might as well put a few drops of the spore syringe on to agar plates first and transfer the mycelium to Lc media

[@ZepHoffman]

@@SporenSprout many thanks bro!

[@edwardhicks3003]

​​@@SporenSproutwhat's a lc media obviously I'm a noob I wanna do spore syring into culture to but don't want contam

[@jackmccourt1367]

I used this exact recipe and mine came out clear and no sediment but has a yellow color I used karo light corn syrup is that normal 😅

[@SporenSprout]

Sounds like you might have overcooked but it should still be fine. Make sure the water level in the pressure cooker is touching the bottom 1-2 inches of the jars while pressure cooking.

[@RipperYouTubeOfficial]

Ok mate, I bought some of your liquid culture from you, and have made this mix in this video. How much liquid culture would you recommend me using for each jar of LC?

[@SporenSprout]

I use 2-4 ml

[@RipperYouTubeOfficial]

@@SporenSprout Thanks, I appreciate the fast response! Also, what's the chemical symbol on your wrist?

[@SporenSprout]

@@RipperKZbinOfficial No problem ! It’s serotonin.

[@orionsbelt3827]

Do you know the measurements by weight for LME and peptone? Asking because I don’t have measuring spoons.

[@SporenSprout]

Just use .5 grams peptone and 1 gram of LME. Mix the ingredients into cold/room temp water not warm/hot water. It this video I used less because I was mixing with warm water and that made it cloudy.

[@orionsbelt3827]

@@SporenSprout dude thank you! One last question. I received some LC from you and I made a rookie mistake of putting the pink oyster in the fridge with the rest of them. Luckily I got it on to agar before I did that and it’s looking healthy but I’m wondering if I should toss the syringe because I’m out of clean agar plates to check if the LC survived the cold. Think it’s done for?

[@SporenSprout]

@@orionsbelt3827 The pink oyster culture will be fine, it is very strong.

[@orionsbelt3827]

@@SporenSprout 🙏🏼🙏🏼

[@richardbinkley8487]

How long/often should LC be put on the magnetic stirrer?

[@SporenSprout]

5 minutes every other day is good.

[@richardbinkley8487]

@@SporenSprout I appreciate the info!

[@b2manufacturing]

Is that filter tape on the lid or is it completely sealed with the exception of the injection port

[@SporenSprout]

The lid has 2 holes. 1 hole has RTV injection port and the other hole is covered with 2 layers of micropore tape.

[@b2manufacturing]

@@SporenSprout awesome! I like it

[@mircoianniciello9452]

I am not capable of fully dissolve the ingridient and still look cloudy, any suggestion? I am using lme, bovine bone peptone and glucose syrup ( didn’t find corn syrup where I live)

[@SporenSprout]

Make sure the water in the pressure cooker is touching the bottom 2” of the jars while cooking.

[@mircoianniciello9452]

@@SporenSprout I will try , thank you

[@ChristophDemmler]

Thanks!

[@applejuicecaboose]

Any idea if those peptones are considered a GMO? Or if using it would mean my mushrooms are now a GMO? Not really something I'm edumicated on but I'm cooking oysters like Mushroom Heisenberg over here and want to start selling these bad boys off. Thank you for the recipe! Literally back right now from months ago because I'm about to cook up some more LC for a new batch of shroomies!

[@tonybahama6817]

I use this same recipe…it’s the bomb. One gram of each to 600ml water 👍🏼

[@PPP-on3vl]

How is it possible that so little amount of sugar works far better than let's say 4% ?

[@MattRoadhouse]

The Myc actually runs for nutes, instead of being surrounded by tons of sugar. Think it helps expand better @@PPP-on3vl

[@nelisv7218]

Do you realise you use about 20 times the amount of what he shows in the video??

[@tonybahama6817]

@@nelisv7218 nonsense. Other growers verified & I got the recipe from an industrial grower. Had spectacular results & no contam.

[@villhelm]

Because most sugars like corn or honey you would use at a 4% solution. LME is a complex carbohydrate and can be used at far lower concentrations such as in this video

[@robertsalazar5459]

only pressure cook for 20-30 mins @ 15psi? I thought it had to be 90 or is that just for grains. Thank you

[@SporenSprout]

@@robertsalazar5459 Yes only 30 minutes for LC media, 45 mins for agar, 1.5-2 hours for grain/substrate.

[@robertsalazar5459]

Thank you, thats foing ro save me alot of time! ​@@SporenSprout

[@vinizuh]

You definitely need the corn syrup, without it, the mycelium will stall less than half way full.

[@fattymcbastard6536]

I'm running this experiment right now, and have jars of LC with added honey. They're 2 weeks old now, and all seem to have stalled. The added sugar hasn't seemed to make any difference on rate of growth or the volume of growth.

[@waynegreenway5445]

Is this better than your honey recipe for Cordyceps Militaris? I ordered some syringes from you last week and plan on making the LC before they get here. Thanks. Love your videos.

[@SporenSprout]

It works well but if you don’t stir it everyday the mycelium tends to clump and tangle which can be annoying. I prefer honey LC for that reason.

[@waynegreenway5445]

@@SporenSprout I do have a stir plate if the LC is better suited for propagating the Cordyceps. Thank you!

[@seanpinnell4806]

Thanks so much, is it possible to convert the tea and tablespoon measurements into grams per 600ml?

[@SporenSprout]

55 mg of each powder and 20 grams of syrup.

[@SporenSprout]

@@pouyarezaei5976 Yes, you can try adding more but if it produces sediment than put 55 mg of each.

[@yullopez1728]

how much in weight is it 1/64 spoons of DME and peptone?

[@SporenSprout]

Just use a gram of LME and .5 gram peptone and make sure to use presto 23 quart with 3 liters of water in it. I used low ingredients in this video because my all American pressure cooker always ruined my media by making it cloudy and I didn’t realize that for a long time.

[@LoveWins]

This might be a dumb question but I'm a newbie so please don't make fun of me lol. Is it possible to take liquid culture that has already been formed, and inoculate another jar with it to allow it to grow? Essentially can you use one jar to make more jars? Also, on a side note, that is some absolutely beautiful looking liquid culture you have. Nice work! 👏👏👏🙌❤️

[@SporenSprout]

Yes you only need 1-2 ml of liquid culture to make a whole new jar and you can keep doing that for a while.

[@bully1676]

4:05 What Fahrenheit exactly is a "cold" room

[@SporenSprout]

@@bully1676 60-65F

[@ZeroRacer]

Would using the mycelium in here be ok to use in an extract? Instead of like using the mycelium from rice?

[@SporenSprout]

Yes this is a great way to harvest mycelium without grain filler.

[@AtanasMukoski-ji5qe]

Hello first time i make the LC. Will you be kind to chek the video of mine and tell me is it good? Can you tell by just looking at the LC ? Thank you so much

[@Asefree3]

😂 I just realized I brought liquid culture from you lol

[@SporenSprout]

That’s awesome! 😆 Thanks for the business and for watching! 🍄 Good luck!

[@sudankarthik507]

Is it distilled water brother?

[@SporenSprout]

Doesn’t matter. Just make sure the water level in the pressure cooker is touching the bottom 2 inches of the jars while sterilizing

[@momsmushroomsjodyfoster5786]

Is time for growth the same regardless of what mushroom you’re growing? For example I thought Cordyceps were ready after like 4 days tops?

[@SporenSprout]

With this recipe the mycelium initially grows faster but will not completely fill the jar like other recipes with more nutrients like honey. Cordyceps can grow for 1-2 weeks

[@Biokemist-o3k]

I love your videos my friend!!!Have you tried sparging with filtered air?

[@jayhovah5621]

Just curious, could you explain why this Recipe works with just a fraction of the nutrients vs all of the other ones that use grams of this stuff?

[@SporenSprout]

The low nutrients force the mycelium to quickly expand in search of more food but won’t grow as full. You can use more nutrients and that’s fine. I just had problems with cloudy media when using lots of nutrients but then I found out that my all American sterilizer is what was causing the cloudiness so now I only use my presto 23 quart pressure cooker for LC media.

[@safwanajasmin7352]

Is this sterilisation is 30 minutes at 15psi? .should we Weight until the pressure cooker turns to 15 psi and the 30 minutes or all together 30 minutes from the minutes I started pressure cooking?

[@SporenSprout]

Yes 30 mins once it gets to 15 psi.

[@czil3984]

is a magnetic stir plate just for convenience or can i just shake the jars

[@SporenSprout]

You would have to have a special hydrophobic filter instead of micropore tape. And shaking won’t really break it up that good.

[@mjarami3]

Hi, I appreciate your concise and simple instructions to create liquid culture. What is the suggested time to let the culture grow mycelium or is it by sight? (i.e. 50% of the liquid has mycelium) I'm trying to build up my culture backlog and extend my spore purchases. Thank you!

[@SporenSprout]

Yes I’d shoot for 50% and it usually takes 1-2 months

[@Cy2o9]

°Super LC recipe

[@Wow88z]

So I didn’t use an agar plate, but I haven’t used my spore syringe yet, so I still have time to buy one - how would I transfer it from agar plate to liquid culture ?

[@ZCRAIZED1]

It's very easy if you haven't already figured it out. You just cut the area of agar that you want and drop it in the jar.

[@Lulzswag]

I've been using that same recipe for a couple of years now. It's the best imo. That peptone really makes the mycelium POP.

[@emekaalex288]

How do you use those syrups to grow mushrooms

[@Lulzswag]

@@emekaalex288 Tht syrup provides food for the mycelium to consume and grow in within the liquid. That's what a liquid culture is. Then you just draw some up in a syringe and use the LC to inoculate grain.

[@emekaalex288]

@@Lulzswag I grap now... Thanks

[@PandemicCild]

Question so Ive seen online that people have said you only need a few drops of a spore syringe to 1ml. So what is the truth can you get away with less ? Or do you need more spores

[@SporenSprout]

A spore syringe is not perfectly clean so you should only use 1 ml. If you germinate and clean the spores on agar and make liquid culture from it than you can use 2-5 ml

[@GwrecksMachina]

can you please do a conversion to grams and milliliters?

[@DG-cv6jf]

I second this

[@alexgreen8846]

Been looking for this recipe, i believe this is the recipe used in the premade jars i bought.

[@esad2077]

y0.... in your video you use 1/2 tsp = teaspoon corn syrup and then the written recipe calls for 1/2 tbsp = tablespoon? Which is correct? kinda confused... could be me.... Please advise~! Thanks~!

[@SporenSprout]

@@esad2077 Sorry, 1/2 TBS is good.

[@esad2077]

@@SporenSprout Thanks for the ULTRA QUICK REPLY~!!!!!

[@P_Tree]

I'm confused too because I thought you said you was 1/64tg of a teaspoon ​@@SporenSprout

[@P_Tree]

Yeah you said 1/64tg of each ingredient. That's not enough food ?! I would think. Now I can see a half a tablespoon of mea and 164 teaspoon of Pepto because I know pepto and goes a long damn way I just actually got some from Amazon I got some soya for 15 bucks

[@SporenSprout]

@@P_Tree Yeah because the lack of nutrients makes the mycelium stretch faster looking for food. You can definitely use .5 TBS of mea and 1 teaspoon of peptone and .5 tbs of syrup.

[@SERGEYFT]

Hello, my name is Sergey. Tell me, do you have experience storing mycelium on slanted agar in a freezer (-20 C)? My experience can be said to be 50/50. Half came to life within a week and the other half did not. I began to suspect that half of the mycelium was lost not due to freezing, but due to drying out. What experience do you have in this matter? The result is the same both on grain and on agar.

[@SporenSprout]

I don’t store my slants in the freezer. 30-40 F is good and prevents excess drying. Dried mycelium should rejuvenate on wet agar pretty easy though. I have had liquid culture at 60-70f that was perfectly fine after 4 years! So maybe you will want to store in liquid instead.

[@SERGEYFT]

@@SporenSprout Storing mycelium in liquid for four years is a fantastic result. Thank you .

[@jobbingactor]

Couple of questions. What happens if say 10% LME is used? and 2. How does the amount of LME affect the long term shelf life of LQ?

[@SporenSprout]

@@jobbingactor you can use more ingredients as long as you make sure the water level in the pressure cooker is touching the bottom 2” of the jars while sterilizing. The amount of nutrients does not have a big effect on shelf life.

[@jobbingactor]

@@SporenSprout Credit where its due your one of a tiny few you-tubers that takes time out there day to post replies and on top of that, very quickly. It doesn't go un- noticed. Message received. Ive prepped some jars very similar to yours, I had Malt yeast extract but no peptone so went for a gram of yeast nutrient. Yesterday's jars were clocking on for 20g per litre so I watered those down with distilled water to about 5% and will PC them again.

[@SubFlow22]

I use the same ratio of peptone but it always turns my LC yellowish.

[@SporenSprout]

That’s ok 👍

[@rogergamer2108]

Have you noticed any significant differences in using corn syrup vs without?

[@SporenSprout]

There is no significant difference. You can leave it out.

[@Jonesybones42_o]

So does this make magic?

[@SporenSprout]

@@Jonesybones42_o If you want

[@calichojack991]

So with this once you on your last syringe could you just repeat this process all over again?

[@SporenSprout]

Yes

[@rhb30001]

My presto states to fill 3 quarts of water when using it.. does the LC recipe glass mason jar have to sit completely above the water during the 20 minutes or is it ok if the bottom portion of the mason jar is submerged in the water?

[@SporenSprout]

An inch or 2 covering the bottom of the jars is normal. You can use jar lid rings to help raise the bottom rack further if needed.

[@leftyB82]

That is awesome I'm going to try it

[@SporenSprout]

It’s a game changer!

[@leftyB82]

Thanks for the info

[@TheManagingTechnolog]

I just prepared the liquid culture for the first time - once is the nutrient broth sterilised and innoculated (I used mycelium from growbox, hope no contam), where do you guys store it please? Shall it sit for the first two weeks outside or inside the fridge please? I understood once its fully colonized its best to rest in the fridge, but I am struggling to figure out, where to store it for the innitial growth period - i will now store it outside, hope it will not eat my house overnight😂

[@SporenSprout]

Yeah just store in on a shelf in a warm room.

[@TheManagingTechnolog]

@@SporenSprout thanks, will do that 👍🏻

[@CryingPlanetDotCom]

I tried this exact recipe which looked amazing and the only thing I didn't do was use your brand of malt. The corn syrup and the pep tone was the same brand from Amazon. I used Anthony's malt and it will not dissolve. Right now I'm not sure if it's the malt or the peptone but something isn't dissolving no matter what I do with the magnetic stir. They're the same color so it's hard to tell. And guess my only other avenue is eliminating one and then the other to see if they work at dissolving entirely in the liquid

[@SporenSprout]

Increase the amount of water in the pressure cooker. The water should be touching the bottom 2 inches of the jars while pressure cooking.

[@CryingPlanetDotCom]

I did a jar without the Anthony's malt and it dissolve fine with the magnetic stir before pressure cooking. I'll try your suggestion on that with the one that still has Anthony's malt in it

[@aquiferjunkie4413]

Does this work well for any dung loving mycelium?

[@SporenSprout]

Yes

[@stevefrench6576]

I have a question . Do you burp your sterilizer , or simply allow it to run for 10 minutes before shutting the valve ?

[@SporenSprout]

I just let it vent for 10 minutes and then shut valve

[@rabbitroger3512]

This week for starting mycelium grow I need to keep it in dark, worm place?

[@SporenSprout]

Yes that’s ok. Light is not a big problem

[@agnesm.w.5937]

600 ml is too much for me for 1 calture. Does the hot water dissolve the ingredients well enough so I can divide the 600 ml liquid into multiplied jars? Thank you.

[@SporenSprout]

Yes that’s fine

[@agnesm.w.5937]

@@SporenSprout Thank you so much!

[@droidnick]

Serious question... why would you need an expensive hot plate to disolve the peptone $ malt extract? Wont that heat up and disolve during the PC?

[@SporenSprout]

I didn’t use a hot plate but some people do.

[@droidnick]

Love this tek, brother.

[@coolaechardonnae7830]

I wonder even grinding down Morning Glory Petals to absorb in with Culture would help with Potency.

[@SporenSprout]

I love morning glories!

[@SERGEYFT]

Good afternoon . What do you think about replacing petri dishes and test tubes with glass flat-walled bottles? It is essentially a hybrid of a test tube and a petri dish. The only thing that worries me is the convenience of isolating the mycelium strain in a bottle. I would like to hear your opinion.

[@Noah-xs6cj]

when it is ready to fruit what do i use for the substrate do i have to use grain and inject it or can i sterilize a plastic bin with cardboard or something else please let me know

[@SporenSprout]

Make some grain spawn: How to Make Sterilized Rye Grain Spawn for Growing Mushrooms kzbin.info/www/bejne/q3e0o2WAndagfZY

[@sigaint666]

How do you get it so clear. All my LC caramelizes 😢

[@SporenSprout]

Use less ingredients and only pressure cook for 20 mins

[@sigaint666]

Do allow to vent for 10 mins or 20 minutes total?

[@SporenSprout]

@@sigaint666Cook for 20 mins and release all of the pressure

[@dtrotteryt]

Your results are fantastic looking, and I LOVE the simplicity of your recipe. Most I have read and tried are far overly complicated in comparison. I can't wait to try this out! Cheers!!

[@SporenSprout]

Feel free to add more ingredients, you can use 1 gram of LME and .5 grams peptone for stronger solution. Just make sure there is enough water in the pressure cooker so that the water level is touching the bottom 2 inches of the jars while pressure cooking. I used low amount of ingredients because I kept getting sediment but found that I wasn’t using enough water in the pressure cooker which caused the sediment.

[@ourfamilyoutdoors7331]

If myc appears flat floating at the top or sunk to the bottom is it dead?

[@SporenSprout]

It is normal for the mycelium to grow on the surface and at the bottom.

[@mycologyromance]

You used corn syrup in the video but said it's optional. Does that mean there's no need of the corn syrup?

[@SporenSprout]

Yeah you can just use lme and peptone

[@funnyguy8638]

I dont have light malt extract but can i use only peptone and syrup?

[@SporenSprout]

Yes that would be ok. Or use just 1 TBS of honey.

[@michaelweimer2499]

Someone please tell me what the red injection port stuff is called

[@SporenSprout]

RTV silicone: amzn.to/4aFLVQ8

[@ajjybaquial539]

what is the alternative when i dont have peptone? peptone is expensive in my country

[@SporenSprout]

Beef extract or vegetable extract

[@SazzadAli-u1g]

So the idea is to sterilize the f2ck out of the jar and fill it with some liquid carbs? Would you consider trying a really crude version of this? Like wiping down the jar with some off the shelf isopropyl alcohol and then dumping some sugar water in with some innoculant? Idk I think it'd be cool to see if it works. Anyway, really cool footage.

[@ArnoldRamirez-c4q]

Karo vs corn syrup ? Or what’s the difference ? Thanks in advance love the videos !

[@SporenSprout]

Not much different other than the karo most of the time has vanilla flavor added to it

[@ZCRAIZED1]

I read the ingredients and saw both types of Karo have vanilla. I wondered if that made any difference at all. I was concerned it might caramelize but I had no issue.

[@mo3taz_mizo38]

if I'm using half gallon mason jar should I still PC at 15 psi for 30 mins or go longer than 30 mins? also if using spores syringe, how many cc should I use for the half gallon jar if I need fast colonization?

[@SporenSprout]

30 mins is still fine. 1-2 ccs although spores are not guaranteed clean. Best to germinate on agar first and then transfer to liquid. You can do it though, just make sure to test a drop of the final product on agar.

[@mo3taz_mizo38]

​​@@SporenSprout doing agar currently is not possible, I got those spore syringes from a very reputable supplier that I had successful spores to grain 10 years ago from their syringes, I'm also following a very strict hygiene including sterilizing neddles in PC and inoculating in well sanitized closed area and using self healing port like the one in video and for the GE I'm using 4× 1/4 inch holes and on each hole I'm using tyvek and on top of it GE filter disk..

[@SporenSprout]

@@mo3taz_mizo38 You should be fine but you can easily buy pre made agar plates here if needed: amzn.to/42C7ZrX

[@mohammadrezanaziri7782]

Why do some button mushroom seed mycelium grow slowly?

[@SporenSprout]

I wonder the same thing. I think they modified the genetics to be that way so that we cannot clone them easily for ourselves. Better to find the species in the wild and start from there to get a clone. I have to button mycelium now growing that is growing well.

[@earlsark]

The video is very easy to follow... thank you bro, from your guidance I made it. As a result, I get mycelium that floats lightly, so clean and smooth like a cloud in a solution that remains clear until the end of the process. Unfortunately I don't understand how to share with friends here. once again, I thank you very much..

[@SporenSprout]

No problem! Thanks for your feedback 🙏 I’m glad you like how clear and fluffy the mycelium is with this method.

[@RipperYouTubeOfficial]

Which model pressure cooker are you using? The 921, or 925?

[@SporenSprout]

I have the 41 quart, 75x electric all American

[@RipperYouTubeOfficial]

@@SporenSprout omg, that has to be a beast! Does that run on 220?

[@SporenSprout]

@@RipperKZbinOfficial yeah I love it! 120 volt