How To Grow Unlimited Mushrooms With Liquid Culture!

[@austinmoreno8486]

Red rtv high temp silicone- Lowe’s/homedepot/any car place. Use this instead of the tape and it’s a self healing injector port

[@phuckyoutube5927]

It's a beautiful thing to be honest

[@DAAI741]

But doesn't this eliminate air exchange? I thought that was the point of using micropore tape

[@austinmoreno8486]

@@DAAI741 drill a separate hole for either poly fill (have to be careful not to get that wet) or look up hydrophobic syringe filters and google that with mushroom and you should find the size you need and videos on how to drill and attach to canning jars

[@lovebig222]

I love that u simplified things with a single hole. Not ideal for larger scale production but so simple for the small home farms. Thanks

[@hailanj]

Out of all the videos I've watched on liquid cultures this answered all my questions and was the simplest to follow, thank you! 🍄🌱

[@SporenSprout]

No problem! Thanks for watching, Check out this updated video! kzbin.info/www/bejne/gIrPkIikjrCSobc

[@captaingildor6884]

Would have been great if you showed that 2 or 3 week later image of a successful jar at the very end.

[@SporenSprout]

yes that would have been a good idea. I have a video of very think growth from this technique i can show you.

[@SporenSprout]

@Somsouk Sourivong www.etsy.com/SporenSprout/listing/857369035/oyster-mushroom-liquid-culture-syringe?Copy&ListingManager&Share&.lmsm&share_time=1603999711267

[@SporenSprout]

@@themyceliumnetwork what?

[@SporenSprout]

@@themyceliumnetwork Well if you buy 7 it’s free shipping I think

[@SporenSprout]

@@themyceliumnetwork I see, I think I have free shipping for international turned off. I can turn it on for you

[@TheTubejunky]

NOTE: If there is more than 4% sugar to water ratio the liquid can be come toxic to the mycelium/spores. It's better to use less than more if you decide to guesstimate.

[@pacificnorthwestfreshmushr930]

Agar Is much easier to trust.

[@contambrah]

What's the minimum sugar concentration?

[@georgekatsinis5224]

@@contambrah If the maximum is answered above, isn't the minimum, by default understood to be ZERO - but with no food = no growth. Anything greater than ZERO will allow for reproduction.

[@somethingforeveryone4206]

Also keep in mind that with less food available you will also be triggering rhizomorphic growth.

[@contambrah]

@@somethingforeveryone4206 isn't that what we are looking for ?

[@Raban322]

I admire your patience, the amount of already answered questions (particularly the marble confusion 😆) is staggering. Thank you for the information.

[@SporenSprout]

😂 Thank you for watching!

[@jeff042854]

I’m a newbie at this, so thanks for the instruction…very clear and helpful! My questions: 1) Where/under what temperature and light conditions should I store the jars? 2) For how long can the jars be stored before going bad? 3) Can you use liquid culture (mycelium) from a syringe as well as the spores within a syringe? Thanks!

[@SporenSprout]

Thanks for watching! Store colonizing jars at 70-80 degrees light doesn’t matter but darkness is preferable , once full they will last for 6 months at room temp and can and should be refrigerated to force the mycelium to go dormant for longer than a year.

[@jeff042854]

@@SporenSprout Awesome, thanks. How about “liquid culture” syringe vs “spore” syringe?

[@SporenSprout]

@@jeff042854 Liquid culture is just spores that have been germinated by introduction to sterile nutrient media. Spore syringes are made with only water to prevent them from germinating, they can be store for years before use.

[@SporenSprout]

@@jeff042854 Also a spore syringe contains many different strains of the species. A liquid culture syringe is one strain. So the genetic differences will be wide with spore syringe.

[@HGICQueenDiamondAries]

@@SporenSprout I did broke boi Tek about 2 weeks ago. I have vermiculite ready for the monotub with all the alcohol to get it ready. I took 11 cc's and divided them between six bags. Some of the bags look like they're about done and some look like they're about halfway done. How do I know when to move the cake to the mono tub? Should I do each one as it's ready, or do I wait till they are all done? I'm going to be watching your videos because this is the first one that I've seen, but if you can answer my question I would be very much appreciative for it. They are already talking to me, I'm excited!

[@harrisonschwartz565]

Funny I realize I actually bought my initial spore syringes from this channel and didn’t even realize until now. They’ve worked great!

[@SporenSprout]

Thanks for the support! 🍄 🙏

[@strictlyundergroundhiphop5129]

​@@SporenSprout after injecting the jars where do you put them to grow and what temp ?

[@SporenSprout]

@@strictlyundergroundhiphop5129 on a shelf in a room that is around 75 F

[@strictlyundergroundhiphop5129]

@@SporenSprout in the dark ?

[@WhiteWolf1980]

​@@strictlyundergroundhiphop5129 yes

[@HGICQueenDiamondAries]

I'm so glad to fall down this rabbit hole because of this video.... I have lots and lots of questions because I am in the first phase of broke boi Tek, about to move into the monotub. I subscribed for all!

[@stevkuj]

This has become one of my all time favorites. You just saved me thousands of dollars.

[@traviss7740]

Wow.... this is a very good idea to have a plethora of any type of species on hand at any given time. Thanks for the knowledge.

[@sphyre99]

Hello, I love your straight forward teaching style. Thank you. Question: could I follow your spore print method and then directly inoculate jars as you have in the above video?

[@SporenSprout]

Thanks! Yes that would work perfect

[@zxt6788]

Its best to use liquid culture as spores take up to a week or so to turn into mycelium and spores are also usually contaminated so its best to do a brf tek with the spores, get agar, take a piece of clean mycelium from either the middle of a cake or the middle of a mushroom or something and place it into the agar, once it turns to mycelium you can use that to create your own liquid culture, you have a much greater risk of contam and contam is very hard to see usually in liquid

[@TerpyTen]

Don't mind me....Just gonna binge watch your channel while i keep prepping more seed spawn.

[@crankshaftwilly6740]

Great Video. Turned my bad attitude into a good one. Best vid for LC.

[@SporenSprout]

Thanks for watching ! I’m glad it was able to brighten your mood, good luck my friend! 🍄

[@MosesMLam]

Super straightforward no fuss method. Thanks! Some newbie questions: 1. How do you know when the mycelium has grown fully in the jar and ready for use/storage? 2. Can you just throw them in the fridge after for storage? If not, what's the shelf life like before they go bad (and how do you know they've gone bad?) 3. How does the Karo light compare with other mixes with malt extract / dextrose?

[@SporenSprout]

1. It is not definitive, but most aggressive species will completely fill the liquid culture jar with mycelium. Once the jar is full of thick mycelium it should be stored in a cold room or in refrigerator. Some species like lions mane and shiitake will usually only grow half way in the liquid culture jar and are not as aggressive. 2. Storage at room temp is fine for up to 6 months, refrigerated or cold room shelf life is over 12 months. It’s hard to tell when it has gone bad, but a way to test it is to put a drop on agar plates. If it grows it is still viable. If the culture is very old it will be noticeably slower or yields will be smaller and smaller over time. 3. Honey is way better than karo in my experience. Karo produces more thin, weaker mycelium and honey produces very thick, aggressive mycelium. It is good to switch between honey and LME once every 1-2 years to change up food source. Check out my updated video about honey liquid culture: kzbin.info/www/bejne/gIrPkIikjrCSobc How some species produce different color mycelium in liquid culture: kzbin.info/www/bejne/b3WViWecbLWhmac

[@saintdavid89]

Do the syringes or the jars need to be refrigerated at any point, or can they be stored indefinitely at room temperature?

[@RasMajnouni]

The European company i bought spore syringes from say refrigerate is best.

[@soyebravat8903]

do you have a video on a good method to create a grow kit?

[@SporenSprout]

kzbin.info/www/bejne/qmaYemVnmN6Kj8U

[@jonnyquest1120]

Awesome info man!!! I know you said these are for LC syringes, is it the same procedure for spore syringes? Or do the spores need to germinate first? Thanks!

[@SporenSprout]

Same procedure. The spores will germinate inside the culture media and become LC

[@WarmBusch]

If your talking about making your own spore syringe then no it's not the same. You will need a spore print, and you would not add the sugar to the water. There's videos here on KZbin that you can follow.

[@99thmonkeyresonance66]

@JonnyQuest [*Newb here*] what does the "LC" mean? (Trying to learn all I need to know and not just listening to one person so I have more of a non-biased, non-judgemental amount of info thats swayed either way) I appreciate your response in advance😉

[@kamadavana5821]

@@99thmonkeyresonance66 I think it means live culture? I’m not entirely sure myself also new

[@99thmonkeyresonance66]

@@kamadavana5821 yes it does. Thanks for answering me. There won't be TOO to much here on "EwwTweb", about "growing mushrooms" in DIFF/ALT ways- (mainly- bc 'they', NEED you- to "buy from" THEM. Same goes w/ the "Gardening"/"plant industry's". Tbh, its DE-spicable- IMO.) You REALLY, FACTually, DO NOT- "NEED to buy, all the 'special stuff'- affiliated w/their 'operations'." There are MANY, many diff ways to go about "growing your OWN mushrooms"- whether for medicinal/food/fun purposes. A good place to start is the Reddit group! "r/mycology"- I believe its called. Hope this helped.

[@Mesch-87]

Can you use tap water or does it need to be something special, would the chlorine in tap water kill everything?

[@SporenSprout]

Tap water is perfectly fine. Here’s an updated video kzbin.info/www/bejne/gIrPkIikjrCSobc

[@Mesch-87]

@@SporenSprout nice, thank you for the tips, can you use anything other than Karo for the food?

[@SporenSprout]

@@Mesch-87 Yes, In the updated video I use honey. I have another video using light malt extract. Here’s the video for that method as well kzbin.info/www/bejne/mXutcqyBab9gZ9k

[@bethechange4934]

Best tat ever!!

[@testosteroneinc.3800]

🤔

[@spicypunch6029]

So why do i need an agar plate? I could simply buy a LC syringe from the lab and make unlimitided amounts of LC. And if my LC contaminates i could rebuy a new syringe for 15$

[@SporenSprout]

@@spicypunch6029 Yeah that’s correct

[@spicypunch6029]

@@SporenSprout so i could skip agar plates. But If i mess up and all My LC contaminats i have to Rebuy a syringe. Probably very frustrating when all you jars are contamineted bc your LC is not Clean:/

[@SporenSprout]

@@spicypunch6029 Yes it’s best to make a couple agar plates as backups and for testing

[@tomanderson848]

Super helpful. Thanks for you r info! Could maybe make an injection port with high temperature gasket silicone too!

[@SporenSprout]

Yeah this is the bare bones way to do it. Here’s the updated video ! kzbin.info/www/bejne/gIrPkIikjrCSobc

[@masontroglen3413]

Baby bottle tek was a great easy 1. The vid got taken down long time ago... up t use what you want. I like organic honey. I prefer it over karo.. helps not to use too much!

[@wendybagley6775]

Really great viedo, very nice and clear. If I dont have one of these magnetic stirrers can I stir it up by swivelling the jar around with the marble inside?

[@SporenSprout]

Yes that will work

[@wendybagley6775]

I could.not find marbles. Are stones ok to use?

[@wendybagley6775]

Thank you for your response!

[@101ckes]

Really great information for a newbie like myself. Thank you for taking the time to pass on your wisdom. You have given me the confidence to give this a go.

[@Martha_mycology]

👆🏻👆🏻👆🏻👆🏻 Hey look up the handle for good spores,grow kits ,shrooms etc

[@sbpermaculturesilvopasture30]

Hi, can I use the new liquid culture to make more liquid cultures without senescence or fruiting problem I use the third generation to inoculate substrate? ( I want to use it on cordyceps ); thank's

[@bradpotetz9844]

Thanks alot man. I rarely comment but I feel you did a great job. No bullshit just information necessary. Do you feel the stirrer was worth the extra cash

[@SporenSprout]

Yes! Best thing iv ever bought for this hobby! I think it was like $30 on amazon. As others have commented the stirrer actually brings fresh oxygen into the mixture and speeds healthy growth!

[@bradpotetz9844]

@@SporenSprout great ! Thanks for the info.! I will get one since I'm about to give this a shot.

[@SporenSprout]

@@bradpotetz9844 sounds good! If you need any help lmk!

[@shashipatel6885]

Hmm, how would I go about taking a syringe from my Liquid Culture to make a new jar of Liquid culture?

[@SporenSprout]

Use a sterile syringe and tilt the jar and suck up the liquid.

[@rancidhotmail]

Thanks for making this video. How long can such liquid culture last for? Can you store it in the closet or long does in the fridge?

[@SporenSprout]

At room temperature they will last up to 6 months. Store in refrigerator for 1-2 years. Thanks for watching and check out the updated version of this video here kzbin.info/www/bejne/fIuzgJWjd7ZmgdE

[@jorgehabash8690]

Amazing video. Does it work for every mushroom?

[@SporenSprout]

Yes

[@jorgehabash8690]

@@SporenSprout Cool, should I keep it in fridge?

[@SporenSprout]

@@jorgehabash8690 After it’s grown enough you can put it in the refrigerator

[@jasonlarsen4945]

So the "nutrient broth" that mushroom syringe companies use to grow mycelium for the syringes is just watered down corn syrup?

[@SporenSprout]

Yes or light malt extract

[@Dykiboop]

@@SporenSprout bro its very hard to get corn syrup in my country? Any other alternative that i can use?

[@SporenSprout]

@@Dykiboop yes many. Try honey, it’s better than Karo. Also light malt extract powder is good.

[@cuddlybear4524]

Am I safe in assuming that, once your jar is colonized, you can then use THAT jar culture to make more jars of culture for a theoretically unlimited supply of liquid culture out of a single $20(ish) dollar syringe?

[@SporenSprout]

Yes that’s basically how it works

[@jessicalatten7173]

Hi thanks for this video! You said we could use a marble too. Does that mean we need the stirrer machine or would we just shake the jar with the marble in it?

[@SporenSprout]

You wouldn’t need the stirring machine if your using a marble. Just tilting the jar back and forth is good enough for the marble to break up the mycelium that will clump at the bottom.

[@thersten]

@@SporenSprout what if it's a magnetic marble and oblong?

[@SporenSprout]

@@thersten Yes, just be careful not to shatter the jar.

[@jefolson6989]

Got a stir machine for less than $30. Its worth it. Make sure to put the stir bar in before sterilizing, but sanitize the bars first. I think they were the source of contam the first time.

[@FishLubber]

Is the tape you are using plastic or paper? Thanks for making this so clear!!

[@SporenSprout]

Paper tape is best: amzn.to/4awG7cm

[@SporenSprout]

Check out my other videos for more tutorials! This was only the first video I ever made.

[@FishLubber]

That’s awesome. What do you think your contamination rate is for the jars ?

[@SporenSprout]

@@FishLubber Maybe 1 in 1000 jars and only from self error like accidentally reusing a needle or a failed injection port or tape becoming wet.

[@waltonfurnacewoodworkingan8663]

Huge fan and customer. One thing... you are using gas heat and alot of people have electric. Gas heats up faster. What would be the time in the pc for electric?

[@SporenSprout]

The time starts after it’s heated up to 15 psi so it would be the same time. I usually do 25-30 minutes at 15 psi.

[@waltonfurnacewoodworkingan8663]

@@SporenSprout ok. I just did mine. It did turn the solution yellow but did not crystalize. I added the mycelium to it. It just looks like i am collecting pee at the moment

[@waltonfurnacewoodworkingan8663]

@@SporenSprout one more question. Can the stir be ever other day or maybe 2 days?

[@jjmatthewsmatthews8808]

Hi my friend I was just watching you on KZbin and I will say I was very impressed by your knowledge and willing to share with us. My son has been growing mushrooms for me to eat because of the research he has been doing about the health benefits of a few different types.... I have a question. We are in the process of making the jar myzilyume. We are in Utah so it's extremely cold. For two to three months his been trying to get some to grow. My question is how long will the myzilyume last in the jar before it's not good to grow anything. We want to be ready for when the weather changes and it's warm enough to grow them. We were trying for a long time and finally I told him to go ahead and keep the room really warm and let's see what happens. Two weeks later we were enjoying some kind mushrooms lol thank you

[@SporenSprout]

Thanks for the kind words! 🙏 mycelium In liquid culture stored at room temp will last around 6 months to a year and if you refrigerate the liquid culture it will last longer than a year.

[@thegreenroomorganic]

Great video man! Is it possible to use honey instead of corn syrup? Like you do with agar recipes?

[@SporenSprout]

Thanks for watching! Yeah I use honey for all my liquid cultures currently.

[@thegreenroomorganic]

@@SporenSprout thank you for responding so quick brother. I’m going to try this tomorrow, this changes the game for me. Mush appreciated

[@SporenSprout]

@@thegreenroomorganic No problem! Good luck with all of your experiments! 🍄 🧫

[@MikeBoneMusic]

Whats the next move after they germinate?

[@SporenSprout]

Watch the updated method here: kzbin.info/www/bejne/gIrPkIikjrCSobc Once you have the liquid culture you can do this: kzbin.info/www/bejne/p6W5fpWAm5tjhs0

[@nitinroy1331]

Q1. Can sugar syrup made out of crystallized sugar be used instead of corn syrup? Q2. What can be be done if i don't have a magnetic stir? What can be done to instead of that process? Love 💕 from India

[@SporenSprout]

Yes you can use any sugar source you have available. You can just put a glass marble inside the jar instead of magnet.

[@nitinroy1331]

@@SporenSprout put marble and shake the jar to stir it?

[@SporenSprout]

@@nitinroy1331 don’t shake aggressive but yes

[@traviss7740]

I will say that my current jar of liquid culture was made with no magnetic stirrer and no marble and the entire thing is evenly cloudy. I just spun the jar with my wrist at least 1x per day for the past 12 days.

[@jasdipsagu3952]

Where do you store the jars when doing this process for 2-3 weeks? In a cool room with indirect sunlight? Then fridge to store long-term?

[@traviss7740]

@@jasdipsagu3952 Yep. High in a closet, probably ~71 F.

[@zlatkomargeta4788]

cool video. have you used laminar flow hood while inoculating jars? thanks

[@michaelflores9796]

Hello i have a question. Would you inoculate the substrate with the syringe? If so will each bag that is inoculated with the same syringe be a identical. Thanks

[@SporenSprout]

You would inoculate grain spawn with the syringe and once the grain spawn is colonized, mix with substrate. Yes each bag will be identical. I have other videos that show the whole process.

[@michaelflores9796]

@@SporenSprout I actually just found my answer in another one of your videos lol. Wife and I are just getting into mushroom growing so will be being watching all your videos. Thanks for all your hard work producing these informative videos. Top notch.

[@SporenSprout]

@@michaelflores9796 Thank you for the kind words! Growing mushrooms is the most rewarding feeling. It feels great to help other people find the same happiness I did. If you ever have questions you can reach me directly on sporensprout.com Have a good night!

[@MonkeySpecs301]

do you keep the jars in the fridge or cool dark place, and when ready use a syringe to extract? or do you make many syringes as soon as the jars are full of mycelium?

[@SporenSprout]

Keep jars around 75 degrees while colonizing. Once a considerable amount of mycelium has grown, you can store in the refrigerator or room temp and extract many syringes at once or whenever.

[@bigshoots1181]

@@SporenSprout idk if you mentioned it, but it's there a expiry time amount on the liquid jar cultures? There's different ways of catering on the species, do you think Agar plates or liquid culture like you do is the best/most efficient?

[@SporenSprout]

@@bigshoots1181 LC is good for 6 months at room temp and over a year if refrigerated. Agar plates are very good for long term storage.

[@bigshoots1181]

@@SporenSprout awesome ty for the info, subbed!

[@adrianmartinez5312]

Great work. Very sterile and described each step very easily to understand🤟

[@marvinwilliams7938]

Hi there, thanks for the video. How many generations of LC can you use before it goes bad? Like you apply LC syringe into LC jars, then you use a bit of the solution from each LC jar to make another LC jar. Usually how many times can you do this before we have to start again? How do you even start again?

[@sptheartist1803]

I swear you can find absolutely ANY tutorial on YT !!!

[@SporenSprout]

KZbin is awesome!

[@sptheartist1803]

@@SporenSprout Just added two items to cart and favourited your shop as well 👍

[@SporenSprout]

@@sptheartist1803 Thank you so much for your support!

[@gandalf2256]

Can you do the same thing with a spore syringe?

[@SporenSprout]

Yes but it’s better to go to agar first from spores because the spores could be dirty.

[@gandalf2256]

@@SporenSprout ahh okay, didn't think of that. Thanks for your reply.

[@chrissladden4593]

Good Vid, clear precise informative presentation. Very helpful. How do you recommend storing the new LC? Thanks

[@MrJoshmmm]

Silly question but I’m a newbie … once you got your jars filled with liquid culture can I just fill empty syringe with the sweet goodness and just add to a substrate and get mushrooms … thanks for the video too !!!

[@SporenSprout]

Yes that’s basically how it works. Check my other videos and you will understand quick

[@jesidillon4593]

Loved the vid I'm i'm a 3world country atm and corn syrup is near impossible to get by. Could I replace it with honey, molasses, etc? Thanks

[@SporenSprout]

Yes honey is the best thing to use.

[@Its_me12759]

I'm from India This vdo is very useful Thanks

[@HoneyandMyco]

Can you do a video showing how you would extract it into a syringe that you could sell or give to a friend?

[@SporenSprout]

Yes I can do that. I’ll start working on it ASAP. In the mean time, quick sneak preview would be to tilt the jar of colonized liquid culture and inject empty syringe and suck up the culture that way. The only problem with that is, if your mycelium isn’t very dense and full in the jar it will be hard to reach. If that happens, try using LME recipe l (I will be making a vid on that soon too). Or you can super sanitize your work area and also use a UV-C light to sterilize everything and then simply open the jar lid and suck the culture up and close the lid right after. Hope this helps.

[@HoneyandMyco]

@@SporenSprout yes this helps but I’m also trying to figure out what syringes you purchase for this exactly like you order the syringes, extract the liquid culture and the replace the syringe with a stopper under a flow hood and then send a sterile packaged syringe to a buyer along with the stoppered liquid culture correct? Also when is your LC considered colonized enough to start extracting it? I have a weeks worth of lions mane LC that I stir everyday. When can I start extracting it? Should I wait a few more weeks of mycelium accumulation? And also how do I prevent it from forming hyphal knots?

[@SporenSprout]

@@HoneyandMyco order empty sterile 10 ml syringes from Medvet international (they come with needle attached) and a separate order of 18 gauge sterile needles, and syringe caps. Open the syringe package near flow hood or in SAB and suck the culture up, take needle off and replace with syringe cap. Now the syringe is ready to be labeled and packaged along with one sterile needle. That is all the friend or customer would need. One capped syringe of culture along with one sterile packaged needle. There is no set maturity to the Liquid Cultures. As soon as there is enough to fill the syringe with a decent amount it is good to go. Usually takes me about 1-2 weeks for adequate growth . Use a magnetic stirrer to insure they mycelium can be broken up and distributed for faster growth and also fresh air sucked in my vortex it creates.

[@HoneyandMyco]

@@SporenSprout thanks that helps a ton... I’m on medvet right now, there’s several types of 18g needles of varying thickness. Do you have a link to the specific ones you use?

[@SporenSprout]

No problem! Type in SYRINGE,10CC 18 X 1, LL, 100/BOX, EXEL in the search bar

[@angelah8247]

I'm your 700th subscriber!

[@mlparts8466]

Great video man! One question: after all that, what's the best way to "duplicate" the LC? Should I take a few CC from the jar and pour them in other LC jars? How many times could I do that?

[@SporenSprout]

Yes you can put a few ml in more LC media or you can put a few drops on a bunch of agar plates to multiply that way. There is no definitive answer to your question, it can be done many times until it stops performing well from old age, some species degrade quicker like cordyceps.

[@mlparts8466]

@@SporenSprout Thank you very much 🙏

[@thedrakesbrah]

hey im back again i didnt even realize this was you i watched your channel since the beginning and didnt even know

[@SporenSprout]

Thanks for watching! 🙏

[@danjf1]

total newbie here.... am wondering so say not to go spore to liquid culture but to agar first. Any thoughts on this? great tutorial!

[@SporenSprout]

If you send spores to liquid culture, the liquid culture will have many different genetics so your fruit bodies will be not very consistent. When you send spores to agar you have the ability to choose the best growing sector of mycelium and transfer to a new plate. Then you can use a wedge of the new plate to send to liquid culture. Then your liquid cultures genetics will be more consistent. Not a big deal either way though. After replicating liquid culture many times you will have to start back again from spores to keep the culture fresh and strong.

[@Itsallgoodd]

I've got a small cloud forming in jar....now to keep it going, how warm should the room be for it to thrive? It's cold up here in Oregon, should I get a heat pad?

[@SporenSprout]

Nice!! You should stir the cloud every few days so that it breaks apart into tiny pieces. I use a space heater in my room and set it at 70-75 F

[@orionsbelt3827]

Hey brother thanks again for the content. I have an extra LC jar of Cordyceps but I’m not ready to use it right now. I’ve been told cordy LC doesn’t store well but I was hoping to hear your thoughts on how I can make this LC last a few more weeks.

[@SporenSprout]

The best thing to do is keep it in a cold room. It will be ok there for 1-2 months.

[@orionsbelt3827]

@@SporenSprout thanks!

[@tsymnz66]

Do I store it in the dark and at what temperature is best?

[@ricknelson9422]

Hi thanks for the video. I couldn't find my question in all the other comments, here it is: when storing the jars in the fridge do you put the jars in something like a sealable plastic bag to protect them from any nasties in the fridge, or do you just put the jars in the fridge as they are? Thanks

[@SporenSprout]

In a perfect world, you would have a dedicated mini fridge just for culture but to answer your question, I don’t use a plastic bag and I don’t know that anyone else does. If the liquid culture is super thick and full like it’s supposed to be, there is less room for contam also.

[@pawemorawski155]

i love this vid this is so understable, thank you for doing stuff like this and i have one question - can i do this also with copelandia cyanescens culture??

[@SporenSprout]

Thanks for watching! And yes you can !

[@Itsallgoodd]

Alternative to pressure cooker? If I do not have a pressure cooker

[@SporenSprout]

Steam bath for 2.5 hours

[@giuseppejones1554]

is there any specific protocol or details for using/transferring/storing the new liquid cultures? also how long does it take after inoculation for it to be usable?

[@SporenSprout]

You can watch my other videos for information about how to use the liquid cultures. A syringe is used to extract the liquid culture after 2-4 weeks of growing in the liquid. Can be stored for years at room temp or refrigeration

[@giuseppejones1554]

⁠​⁠@@SporenSproutsince commenting i’ve watched a lot more of your videos and they’re great. you’ve done us all a great service so thank you. that’s surprising they can be stored for so long even at room temp!

[@calebwoodruff984]

Hello! Would there be any way to possibly spare a few mushroom caps and mushroom nutrients put them into a blender and use that mixture to grow more mushrooms?

[@SporenSprout]

Only if you are inoculating some wood chips outside in the forest or something. Look up the term “slurry” for growing mushrooms.

[@Batsnang]

Quick question and I'm sorry if this has been asked: could I use a simple (sugar) syrup instead? I don't have corn syrup on hand but I do have sugar, water, and a pot. So I figure I'll ask!

[@Kay-rh1jb]

Honey. Pure honey

[@SporenSprout]

Yes just use honey

[@eskee1]

Sugar will work

[@crazygandalfwithweed1180]

@@SporenSprout brother, do i substitute honey and corn syrup in a 1 to 1 ratio ?

[@SporenSprout]

@@crazygandalfwithweed1180 Yes

[@thekarmafarmer608]

I agree with the comment below. Great, straight forward style of teaching. Thank you for posting this

[@SporenSprout]

I appreciate that, thanks for watching!

[@josephrikers1111]

Also I’m assuming you have to first by liquid culture strains? Can you do it with spores from the caps of ready to pick shrooms?

[@SporenSprout]

You can use spores but you will have many different strains growing. Also spores are not entirely clean so you may have to test the final product on agar to see if it’s clean.

[@nicgal1735]

I have 2 larger white fuzzy jellyfish floating on top I stirred 2 days before and now they appeared, it’s been 13 days sense I injected with LC and mycelium growth is at 400ml when settled very new first time trying jar and I can’t really find an answer if it’s good or bad? Or contaminated? Thank you

[@SporenSprout]

The mycelium will start growing on the surface like that when it has used up all nutrients

[@nicgal1735]

Thank you

[@Gorguruga]

Great video! I don't have a pressure cooker or a magnetic stirrer. I know they're widely used in mycology but I was wondering if there's any way to sterilize and stir without investing in those items? For example a make shift steamer using everyday household pans (instead of the pressure cooker). And simply physically shaking the jars (instead of using the magnetic stirrer). Any broke boi possibilities there?

[@SporenSprout]

You can steam the jars for 2 hours in a pot of water with a lid. You cannot shake the jars if you use a micropore filter because the liquid will touch the filter or even leak from it. You can use a marble and swish it around the bottom to help break up the mycelium instead of shaking or using a magnetic bar.

[@pinkwheat]

Hi, can you sub maple syrup or should it be honey/karo? Thanks, great video!

[@brodiebiology560]

You could use 40grams of honey/L

[@HeyHeyHarmonicaLuke]

@@brodiebiology560 Honey has antibacterial properties! I wonder if that would help with contaminations. "Honey exhibits a broad-spectrum of antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria, including antibiotic-resistant (MRSA) ones. Honey has been shown to have a strong activity against many bacteria in both media and in culture."

[@drty_satyr384]

What do you do with the jars now? Fridge or leave em out?

[@SporenSprout]

Leave em out

[@hitechsolution6022]

What is the difference between this method and your another method of a separate injection port plus air filter hole and pressure cooking with 15 psi for 30 minutes ? Also have a question about the regular 2 piece mason jar lid that rusted the filter hole overnight after i left the honey water in the jar for overnight cool off. i am using your liquid culture syringes which i bought from etsy. Do you think i will have to redo everything over again with plastic lids or it will be still okay.

[@SporenSprout]

The difference is this video is I didnt use rtv silicone injection port. The filter hole was not rusted, you probably splashed the honey water and it came in contact with the filter. The filter should not get wet. Make sure water level is half way in jar, make sure not to over cook too hot, make sure not to splash. If the filter gets wet, dry it off with laminar flow air.

[@timothymccarthy7747]

If you invert your lid and put it in a hot oven it will melt stuck to the ring. That makes it easy to remove when you inject spore solution.

[@BlasterTheMaster]

Awesome video thank you!! How long should I let the jars cool before inserting syringe?

[@SporenSprout]

Thanks for watching! Let jars cool to room temp before inoculation.

[@BlasterTheMaster]

@@SporenSprout sweet thanks for the quick reply, they just got done sterilizing, perfect timing dude!

[@BlasterTheMaster]

@@SporenSprout is it okay if the liquid is a tiny bit yellow, mostly clear but just a small yellow tint is there, barely noticeable

[@SporenSprout]

@@BlasterTheMaster Yes, that’s ok. My favorite thing now is to use half tablespoon of honey and 30 mins at 15 psi in a pressure cooker.

[@BlasterTheMaster]

@@SporenSprout epic, I’ll have to try that next time, this is my first time growing and I just made my substrate and got recommended your video so I figured making the liquid cultures would be perfect so If I messed up I could try again! I heard you have a website and sell spores? I’ll buy from you next time I order some! Thanks for all the help, happy thanksgiving!

[@nexusfxgen3449]

Must corn syrup be used? or can any other type of syrups will do the job ?

[@SporenSprout]

You can use other stuff including honey

[@kingdav5794]

Thanks for sharing. How can the mycélium breath and grow in a liquid substrate while it hardly grows on solid substrate with too much water ? Thank you

[@SporenSprout]

That’s a good question

[@shannagarroutte7829]

I wonder the same thing about how plants can be propagated in pure water and produce nice healthy roots, and then that same plant in soil with too much water gets soggy and the roots rot 🙄

[@ConstantlyCrawford]

Instead of corn syrup can I use honey from my pwn hives?

[@SporenSprout]

Yes check out the updated method here! kzbin.info/www/bejne/gIrPkIikjrCSobc

[@tee228]

my jars look really dense and cloudy with mycelium at the bottom only. is this normal or is it supposed to fill the whole jar? i stir every day and whenever i check, it all settles at the bottom.

[@SporenSprout]

Yeah that’s normal

[@moe40cal]

So is this to make more syringes to grow more shrooms or this will grow the Shrooms? Sorry trying to learn, and how many ML is 2cc?

[@SporenSprout]

This will grow more syringes so that you can grow more mushrooms

[@SporenSprout]

2 ml

[@yakivpopavich]

Is it easier to do this or just use your liquid culture to do grain to grain transfers over and over?.

[@SporenSprout]

This is easier

[@yakivpopavich]

@@SporenSprout Thanks, how long can the jars keep once they are made?. I assume refrigeration increases longevity?

[@SporenSprout]

@@yakivpopavich around 12 months or longer in refrigeration.

[@joshuabarnhart9028]

It looks like you used trasnpore in this video. Idk if anybody else noticed. But, if using a smaller amount of LC in a jar, is there any reason to include a gas exchange port?

[@divinicus]

do you ever experiment with adding supplements to the liquid

[@SporenSprout]

Sometimes I add a pinch of light malt extract

[@treybegood]

Can you substitute with normal corn syrup instead of light (Karo) corn syrup and if so, how would you modify the recipe?

[@philiolynott4886]

Can you add spores to the solution instead of LC? thanks!

[@theresnospo0n]

Yes.

[@SporenSprout]

Yes

[@DamnBrother]

Do you refrigerate the jars after it's fully colonized with mycelium?

[@SporenSprout]

I keep expansion jars at 78 F for 1-2 months and then I move them to a very cold room instead of a refrigerator. I keep master slants in the refrigerator.

[@DamnBrother]

@@SporenSprout thanks for the reply

[@qlanbeats5820]

Hi can you use glucose syrup as a substitute for corn syrup?

[@SporenSprout]

You should use honey! It’s wayyyy better.

[@qlanbeats5820]

@@SporenSprout Wow! Thank you...

[@SporenSprout]

@@qlanbeats5820 no problem! I use 1 tbs of honey per 600 ml of warm water and then pressure cook at 15 psi for 30 mins.

[@kayasamuk]

How can I propagate arbuscular mycorrhizal fungi in this way, dry them and store them? I will use it for the roots of trees in the ground

[@SporenSprout]

You can propagate any mycelium this way and then just spread the mycelium under the trees

[@kayasamuk]

@@SporenSprout thank you 😍

[@silentwalk1768]

Thanks for the video not sure what you meant by the marble you did not show that do you put a marble in each jar like he would a spray paint can

[@SporenSprout]

Yea just throw a marble in the jar before pressure cooking the jars for 30 mins at 15 psi. I used a magnetic stir bar in this video which is preferable

[@josephrikers1111]

How much typically, I know many factors are involved, but with a 10cc syringe, how much shrooms could one grow? Or yield?

[@SporenSprout]

Using 2 ml per inoculation, you can inoculate 5 3 LB bags of grain which you can grow around 1 pound of dry mushrooms from. You can attempt “grain to grain transfers” to spread the mycelium to even more bags of grain and exponentially increase output.

[@adtatatumtemporis7111]

How should it be stored? Light, temperature etc... If stored, how long can ut be stored before it dies?

[@_Mikekkk]

Is there a reason to use a tape instead of silicone to protect that hole?

[@SporenSprout]

It needs to breathe. You can do 2 holes, one with silicone and one with a few layers of 3 m micropore tape.

[@user-xu5vn3tp3i]

So when you extract the liquid culture into a syringe do you have to add another layer of microspore tape ?

[@SporenSprout]

@@user-xu5vn3tp3i Yeah. Check out my newer videos I use better lids

[@doctordabs3051]

where is the best place to store the jars during the 2weeks?

[@SporenSprout]

On a shelf in a room without dust. 78 F temp

[@houriaoug3522]

If we haven't this syrup what can we replaced it by other syrup like honey for example?

[@SporenSprout]

Yes honey is actually better, that’s all I use now. I use 1/2 TBS of honey dissolved in 600 ml of hot water in a quart jar and sterilize for 30 mins at 15 psi. Perfect every time. Here’s a video I made more recently kzbin.info/www/bejne/gIrPkIikjrCSobc

[@Mightybean222]

After watching a few more times this might seem like a dumb question, but should the lid be fully closed when pressure cooking, or slightly opened.

[@SporenSprout]

The lid band is loosened a 1/4 turn. Just to help reduce pressure on the tape.

[@Mightybean222]

thank you soo much, youre the man!@@SporenSprout

[@therealderrickwynne]

Can this same process be done with an already existing culture solution?

[@SporenSprout]

Yes

[@7H3M4DH8R]

How long does the liquid medium last before inoculation?, I got some of my cultures in but currently waiting on more but would like to get all these ready for when they arrive.

[@SporenSprout]

Months. It’s actually good to let it sit for a few days before inoculation just to make sure no other growth appears. Btw you should use 1 Tablespoon of honey instead of karo. It’s much better.

[@7H3M4DH8R]

@@SporenSprout Thanks I'm using honey but after pressure cooking it settled to the bottom of the jar I did mix it well. Wonder if it was crystalized in the bottle. Has this ever happened to you.

[@SporenSprout]

@@7H3M4DH8R Yes this is very common due to over cooking. You should start over, It has to be done very precise. Use very warm but not boiling water and dissolve 1 tablespoon. Pressure cook 30 mins and not a second longer. Try not to go over 15 psi as well.

[@7H3M4DH8R]

@@SporenSprout Funny i did 4oz with 1 teaspoon for 10 minutes. My honey was probably just old. I'll try again with new honey.

[@Birdgangg]

I have two quart jars, one with blue oyster and one with lions mane. The blue oyster mycelium is growing every day and after two weeks, has filled up nearly half of the liquid medium. The lions mane, however, grew a little in the beginning, but seems to have stalled out in the last week or so. Is this an issue with the mycelium or could this just be a species specific issue?

[@CHLNlikaVLN]

If I don’t have a pressure cooker, am I able to boil the jars in a large pot for 90 minutes at medium to low heat?

[@SporenSprout]

I would do that for 2 hours and even then I wouldn’t completely trust it. Test the end product on agar before using it.

[@Aggoenix]

Do liquid culture lasts the same long as spore syringes (literelly around a year or more)?

[@SporenSprout]

Most liquid culture will last over 6 months at room temperature and over 12 months in the refrigerator.

[@littlemissstonerr]

Those syringes that you started with are spore syringes right? I’m wanting to make some lc with several spore syringes that I purchased. I’m just getting confused when you call them “liquid culture syringe”.

[@SporenSprout]

No I used a liquid culture syringe. Liquid culture is basically germinated spores. It is mycelium. You can use a spore syringe the same way as you would a liquid culture syringe but it is considered multispore inoculation and will result in many genetic differences.