Sterilisation vs. Pasteurisation in Mushroom Cultivation I How To Grow Mushrooms I GroCycle

[@GroCycleTV]

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[@aivkara]

I'm a total Newb to mushroom growing in practice, but I've done a lot research. You two have, I think, the best information and approach to real-world startup of mushroom growing that I have seen in several years. Thank you!

[@sirdeakia]

Guys, the most important part of Pasteur's process isn't elevating the temperature to x or y degrees. It's subsequently lowering it to a radically lower temperature, with the variation being the final strike on microbiological life.

[@scroogemcduck1462]

Can you expand on this?

[@vk081064]

Thanks a lot brothers. That's a very detailed comparison study. God bless you folks. Keep safe.

[@arthurbuccioli]

Cheers from brasil, thanks a lot for the video, very usefull info! Whatts the proportion of line to use in cold pastheurization? Can we combine lime and hot water methods?

[@danielsigursson6215]

Excellent video, great explanation and comparison between wet weight harvest yield and actual biological efficiency.

[@stevensanborn6010]

Pretty sure i saw that just sterilization adds yield done in experiments on non supplemented substrates. Not sure by how much , because there are many ways , species and types of sterilization and pasteurization . Also sterilization has a very key benefit of reducing contam. I do both sterilization and pasteurization and I found my contam goes down by a lot using sterilization. Tossing blocks is the biggest waste of energy and just feels bad. This is also why its more consistent , so its important to consider - trust me id rather soak than pressure cook. Also bio efficiency is great for straw because its light and carry so much water. But it takes up a lot more space. So it's also important to consider that if you are a grower limited by space , denser might be better .

[@osmundmathias7998]

happy

[@ebtsoby]

Yessss, highly debated topic, thanks so much for covering this been looking for a vid like this for a while and I trust you guys cause you're pros, keep it up!!

[@ebtsoby]

and the breakdown of biological efficiency at the end was great!

[@saltypyro9458]

@@ebtsoby Not really. They are comparing a hardwood substrate to a softwood substrate like straw. We already know hardwoods produces more BE. That's why we use hardwoods, not softwoods like pine and straw. Pasturise and run a lil bit higher spawn rate to take over faster.

[@bonbalab_]

Thanks for sharing Amazing idea

[@joeyb6680]

Great video fellas. Really good information in this one.

[@DianaMJoice]

I love your detailed mushroom growing videos. Thank you! And... would sterilisation work in an oven?

[@slamurai8080]

thankyou so much for sharing such valuable information!! love you guys!!

[@ignaciaholmes4897]

Hello, great video thanks. I am in Chile and wonder what is the hydrated lime chemical details so I can try to find something similar here

[@chinocio]

Hola Ignacia. Calcium hydroxide is an inorganic compound with the chemical formula Ca(OH)₂. (Wikipedia)

[@jackthemuss2493]

Brilliant guys. Love the math. Also I prefer Hydrated lime and only pressurize sterilization for spawn seeds. The best thing about learning from you guys is it lets me know what I'm doing is correct.

[@kemaketseforkssebitiela3089]

Did you mention vinegar, chlorine and lime for cold substrate pasturization?

[@seanshiels153]

Cheers lads. A lot to ponder there 👍

[@rikjennihoddinott4063]

How do you dispose of the lime infused water after pasteurisation? Would I be harmful to dispose of?

[@ryaeon9793]

thank you! can we have all cold pasteurisation method? lime vinegar etc etc

[@leicodaya4660]

Quality info my bro

[@SparkyHelper]

Such a great video. Thanks. Looking forward to taking your course soon.

[@shawnc958]

Glad to find your channel !! so Im looking at getting into medicinal growing for health, years ago i grew "recreational" mushrooms using a brown rice flower and vermiculite substrate and high heat simi sterilization with great success, any thoughts on this substrate mix ??

[@jaquiwilson5051]

As always very informative. Thank you.

[@thekarmafarmer608]

Hi guys, I have asked a question before in several places but never got an answer, concerning the pressure of pressure cookers here in UK. After this video I`m compelled to ask again in the hope someone has an answer. I recently bought a well reviewed pressure cooker, which has a sterilisation setting. I found out only afterwards that European/UK pressure cookers have an lower maximum working pressure than American ones; around 12 psi. I`m quite disappointed that most videos cite 15 psi as a base line for sterilisation. I gather from this video that my cooker will merely `super-pasteurise` my substrate. I wondered if I could off-set this deficit by adding time to the cook? Any ideas, or will I just have to save for an All American? Thanks for your great work.

[@Juniperlaneestatesales]

12 psi is perfectly fine (google shows many discussions when asked if 12 psi is okay to sterlize) just leave for 90 min to 2 hrs

[@thekarmafarmer608]

@@Juniperlaneestatesales Hi. Thanks for the reply. Yes, I did read many articles and even scientific papers on the subject but all suggest that 12psi would `super-pasteurise` and not sterilize.

[@MrTupimus]

Yes, just takes longer. As a sidenote superpasteurization refers to a technique where pasteurization temperatures are held for 12-24 hours. Works much like sterilization.

[@junbaldonado4055]

After cold pasturization and let the material drip, do you need to rinse it? When you add supplement to enrich the nitrogen content, is the supplement in whatever form, does it need to be sterilized because you already pasturized substrate and an added supplement ?

[@Nostalgiaforinfi]

No rinse.

[@humanjad]

excelente video, sigan produciendo buenos contenidos!!!

[@irilesyataghene1567]

how many times can you reuse the water in the lime pasteurisation method? maybe by adding some more lime at each batch to reach the desired pH..

[@samuel58237]

What is the difference in yield per square foot of building? I guess that depends on how much your columns weigh.

[@CMZneu]

When you mention biological efficiency, how many flushes are you considering?

[@CMZneu]

@@justinlangley9529 Standard?! i don't know, i don't remember seeing any mushroom growing channel that normally get 100% of first flush. Can you recommend one? what substrate do they use?

[@rfldss89]

1:00 do you have any experimental data to back that up or something? I couldn't find any sources starting that the simple fact of boiling a closed container increases its temperature above boiling due to the pressure, and from my understanding that's impossible.

[@alexandervlaescu9901]

This is a really simple physics fact. Boiling , freezing , etc points are affected by two major things : temperature and pressure. Normally when you freeze or boil something you do it in an environment of one atmosphere (normal atmospheric pressure). If you were to have a higher pressure boiling and liquidizing something would be far easier.

[@danushkajayasooriya574]

Great vedio thanks lot

[@trickyblickfang]

what abuot cooking in ashwater? do i may need to wash out with cooking water afterwards?

[@majidaattaplantscare4582]

So amazing

[@ozooner]

Hello, thanks for the detailed video! You mentioned supplementing with nutrition, but can you go into detail with this as well? I know some people add gypsum and some say that adding nutrition is overthinking. What is your verdict on it?

[@WASS291]

2 studies showed that adding caco3 calcium carbonated (which acts as a buffer for ph) and gypsum calcium sulfate (makes the substrate surface better ) so yeah consider using them and do not use hydrated lime or lime which is calcium hydroxide ca(oh)2 and good luck brother :)

[@sogniluigi]

Can I pasteurised sawdust? Because sounds like that i can just sterilised sawdust. Thanks for the good video

[@CMZneu]

You can definitely pasteurize it as long as you don't add anything to it.

[@stefanieshedd9687]

Eric you got your haircut!!

[@bartendersunny1]

Can anyone explain the vinegar method? What % of vinegar to water?

[@MalinaImport]

i believe temps don't need to be 121 for sterilisation. atmospheric sterilisation for longer times at lower temps also works.

[@viktormorris194]

What you're referring to is super pasturization. It functions similar to sterilization when applied to bulk substrate bc it kills, say 99.5-99.9% of contaminants. The mycelium can usually handle the rest. But it's not *technically* sterilized bc of that small amount of bacteria that could survive

[@rfldss89]

For sterilisation you need to reach temps high enough to destroy the most resilient microbial spores. Generally thats considered to be botulinum spores for which you have to reach 120°C for a certain amount of time. Maybe colloquially sterilisation is used in a different way, but we should really stick to technical definitions to avoid confusion.

[@asamusicdude]

Will liquid PH up do the same as lime?

[@dirtpoor9571]

We're you ever able to find an answer?

[@asamusicdude]

@@dirtpoor9571 I tried it and it works fine

[@junbaldonado4055]

Do you have to rinse after cold pasteurization?

[@trip_lord1atinstagram890]

Look him up 👆

[@Nostalgiaforinfi]

No

[@skullbone2473]

What kind of pellets are/can you use?

[@bubblerings]

I think the video is: Easiest way to grow oyster mushrooms at home... They use pre-pasteurized Straw pellets or hard wood pellets.

[@Ted...youtubee]

I realize you respond with "volume", but, why can't one use microwave oven for killing substrate organisms?

[@shayeladshayelad2416]

Hello the im trying to get oyster king/orngi mushroom with no success I have the white furry mycelium but when i drill holes in the box after more then month there arent even small mushrooms poping What can be the reasons for that please help

[@bubblerings]

Watch more videos about Fruiting conditions... and monotubs. You drill the holes first, and can Cover them with tape until fruiting... or you will have problems.

[@victorsenna5729]

Hi, sterilization is relative, you never kill everything. Sterilization reduces 99,9999999999% (log reduction of 12) of organisms and pasteurization reduces 99,9999% (log reduction of 6).

[@ianmoon1103]

Here we go 🙄

[@kennetharmbruster]

He said eliminate all of the COMPETITIVE ORGANISMS. If they’ve been mitigated/debilitated to the point where they’re no longer a threat, they’re not competitive.

[@joshuafritz1386]

For some reason I cannot imagine that sterilization is applicable to rural areas with little to no technology. We are talking about growing in a simple way. Farms aren't clean.

[@saltypyro9458]

Trying to compare straw to hardwood is not a good comparison for BE. We already know hardwoods produce better than softwood like pine and straw.

[@kemaketseforkssebitiela3089]

Use subtitles please

[@ccrust2111]

chestnut is not agaracuis it is pholiota