Column chromatography - gel filtration chromatography lecture

this column chromatography lecture video explains about the protein gel filtration chromatography technique principle. This is also known as molecular exclusion chromatography or gel filtration chromatography. In this chromatography process molecules are separated based on their size. Mixture of molecules are passed through a polymer network made with agarose gel. Large molecules will not enter into the pores created by the agarose polymer so they will elute faster and the smaller molecules will be trapped inside the agarose polymer as a result they will take more time to come out of the column.
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Tq sir u are very helpful to me to understand easy about chromatography.... Tq ...

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[@nikhilsaripalli4085]

Sir What if smaller molecules enter the void along with larger.. its not compulsory that smaller take the pores only, they may enter the voids too. Than first to be eluted is smaller particles in this case..

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[@payalagarwal8444]

I have a query, so, does folded protein comes out first or unfolded according to their hydrodynamic volume?

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Thankio Sir I like Your Teaching method, Your video Satisfied me in my aim and many many thanks at last iwant to say you Sir please explain Uranic Acid Pathway If You can. Thankyou Sir

[@praveenkumarmahto4316]

sir , when we will pour a sample it may contains undesirable materials but we get product according to size. So undesirable materials are also disributed in product according to size. Then how we can separate undesirable materials.

[@laxmibirajdar4667]

Another difference between gel electrophoresis and size exclusion chromatography is that smaller fragments will run faster and larger ones will run slowly in gel electrophoresis while in size exclusion chromatography smaller molecules will come slowly and larger ones come faster, am I right Sir?

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[@malikhaseeb6022]

i want to lecture and assignment on protein purification in detail . I really like your teaching style that,s so good .so, i want to take more lecture

[@nuralam9013]

Hi... thanks for your all lectures..... would you please upload about various types of detector used in chromatography..... thanks again

[@jisansarwar1050]

If I'm using agarose instead of sephadex beads as the matrix of the column then how do the protein of high mol. wt. will elute faster than that of lower ones

[@fatimaimessaoudene4766]

thanks for your big healp . I just look to go deeper in technics.i mean how to do calculations of the V0 or Retention volume and time . how to read a cromatoramme if you can o that I'll be thankfull

[@Jivanam_anubhavah.]

hlw sir , u teach us very smartly . tx for that . but , somewhere i studied that some mixture of protein standards added first before mix of protein . can you explain why is it ?

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Hi SIr, I love your videos a lot...Can you please make a video about chromatofocusing

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+Akshay Zagade you're going welcome

[@senolgundogan4787]

HI! Sir I have this question above could you please help to me solution please. Proteins may be quantified by spectrophotometry at 280nm. Explain the underlying principles of this technique and outline the advantages of this assay over alternative methods.

[@aniruddharanadive3016]

please sir..take 1 lecture.on this topic

[@aniruddharanadive3016]

what is flash chromatography?

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What about Gel permeation chromatography ? Is it same ?🤔🤔

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Yes

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Plz explain affinity elution

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[@talenteducation405]

Is it adsorption column chromatography?

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How to separate sample and mobile phase from the reservoir

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Please make a video of analytical method development in hplc in hindi

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[@medinaafandiyeva6936]

So, gel filtration chromatography is the same as column chromatography? I am little bit confused with terminology

[@gerardreaper8793]

column chromatography covers many types of chromatography like ion exchange, affinity and also gel filtration. so gel filtration is a type a column chromatography since its run through a column

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Thank you so much.

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[@muralitharan2275]

Why take about application of.gel filtration chromotography?

[@dr.mastan]

If sample contain both smaller and larger prtn then how only larger prtns I'll come through pores why can't small prtns come through the same pores from which larger one comes

[@levelone7511]

anthati mastan small particles has two route: through pores and from surrounding with large ones the letter has one so low percentage of small will run beside large

[@krishnendusen1686]

How can be the unit of hydrodynamic volume is angstrom??

[@karanpatel4583]

I though the reason is buckflow not just gravity

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understand easily

[@mohanakaruppiah4587]

From this GPC data how can I find out the molecular weight of my synthetic compound

[@djbheris4991]

I think there is now a technologically advanced set-up where there is a detector such as UV-Vis and refractive index.

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sir elution means

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Elution mean to carry

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[@aravindsr907]

In another lecture... they said larger molecules will travel slower as they want to find alternative way, around the st phase and but small molecules can easily move through the pores in them and travels faster.... Actually which statement is true ??🤔🤔

[@akankshaawasthi8680]

Aravind SR I think the one where large mol. Are eluted first is much more gripping concept because as you see the surface are for the large molecule will be greater and the way they had to find will be small in comparison to the small molecule which are stuck in the labyrinth of pores.....like a ant which has average speed way much faster than a cat but the cat will still outrun them.

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