DNA repair mechanism - Base excision repair

[@guljafari3269]

this video was uploaded in 2014 and here in 2019- it is still very helpful... after listening to hours of lectures- this is more helpful than anything else. thank you very much I hope you don't stop producing more these biology videos. thanks-lifesaver

[@shomusbiologyofficial]

That's what most people don't understand. They mock these headphones but the relevance was ahead of time. Thank you for appreciating my efforts. Glad to hear that you are getting benefit from the videos.

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Thank you

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Really sir,u r explaining all the concept for basic theory which is very helpful for the students to understand the concepts of immunology,human genetics,biochemistry,biophysics...all in one sir..you are legend.

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You're welcome. The best for the exam

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Thank you

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@@shomusbiologyofficial nevermind

[@kiranbhagat6019]

Thank you, sir, you make this video in 2014 in this year I was in 8 th standard and nowadays it helps me when I am in my BSc final year 😁😁😁 thank you so much!

[@daimahamid5078]

Oh my my.... You explain really well.... Thanks for this video.... It was really helpful

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Thank you Sir...🤗💞💞🙂👍👌🤗.. You are my best favorite teacher Sir.... Thank you ..

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@roosheel]

You made a small mistake when you spoke about DNA pol I 5'-3' polymerase activity. The 5'-3' polymerase activity only works when you are using the 5'-3' exonuclease activity, ergo the concept of "nick translation", but when you use the 3'-5' exonuclease, one of two things must happen, either you excise in one direction, and then consecutively, move backwards in the 5'-3' direction to add the new repaired nucleotides. The nucleotides are not added while you move in the 3'-5' direction. Or you could have a distributive polymerase activity, where Pol I disassociates from the DNA and a new Pol I binds and repairs the gap via replacement with new nucleotides. Other than that, great video

[@luisfreitas6435]

So in this case the DNA pol I uses his 3' -> 5' exonuclease activity and add new nucleotides using his 5 --> 3 ?

[@shohanahmed7221]

@@luisfreitas6435 No cut the segment of DNA by using 5'-3' exonuclease activity then siled the gap by the polymerization activity of the DNA pol-1

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You are my saviour; amazing lecture bro

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You are truly a blessing!

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Thank you

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Love from kerala sir thanks for yur videos 😇✨️

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If only my professor can explain this like you do then the class would be fun to learn.

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[@NorthernChinar]

Since you were explaining about E.coli BER pathway. you forgot to mention the role of DNA phosphodiesterase in removing the AP-Deoxyribose phosphate that was left by the DNA glycosylase. & thats the only difference in eukaryotic and prokaryotic pathway of BER. eukaryotes dont use DNA phosphodiesterase.

[@onaizasumreen1165]

Sir you are doing excellent job... It's help me out to understand my Molecular biochemist lectures. You elaborate everything... I like your way of teaching. Keep it up sir 👍👍👍

[@shomusbiologyofficial]

+onaiza sumreen thank you. Glad you liked my lectures. Please subscribe and share

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+onaiza sumreen thank you. Glad you liked my lectures. Please subscribe and share

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I have already subscribed sir..

[@syedaaisha3858]

great sir. seriously your are fantastic ....i really got a lot of knowledge from your all informative vedios

[@nandininandu4731]

Really very nice explanation sir 🙏

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[@margaridar7187]

You saved me again ! Thank you so much!

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Very nice method of teaching...Thanks

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U are really an amazing teacher

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Thank you

[@sandhyasingh9650]

mistakes happen evn by d teachrs also ....iam grtful fr ds coz it help me to clear my concpt ..thank u

[@sherazkhan2802]

DNAP I actually uses its 5' - 3' exonuclease activity there .

[@whatsnew14]

But in eukaryot it's DNA pol beta isn't it? Plz answer

[@cook.king0844]

Very well explained❤❤❤❤

[@shomusbiologyofficial]

Thank you

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Thank you so much sir .. i love all your videos and it helps me a lot !!♥️♥️

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Thankyou so much. Your videos are very helpful.

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You're welcome

[@imMainak49]

Easy way of understanding. Thank you

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[@gouravsaha7031]

Nicely explained... thank you sir😊

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Thank you ,sir, from Sylhet Agricultural University, Bangladesh 🇧🇩

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Thank you. Glad you liked my lectures

[@laddugopal8137]

perfect explanation 🤩🤗

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I love your channel, thank you for everything 😍

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[@phanitej3122]

very well explained ... thanku for ur job...

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Wonderful lectue thankuuuuuuu so muchhhhh

[@shomusbiologyofficial]

You're welcome

[@divyavarshney6222]

Thank you sir for the amazing video .....and sir i have a question that is Does proofreading repair induced mutations ?

[@shomusbiologyofficial]

If mistakes present, it may cause mutation

[@divyavarshney6222]

@@shomusbiologyofficial no...sir i want to know that will it repair or not ?

[@zanobiasohail9337]

Nicely explained. 👍🏼

[@shomusbiologyofficial]

You're welcome

[@Sleepmeditation25]

U r doin awsm job

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Thank you

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Very nice explanation..thank you sir

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That a lot sir. U just saved me up

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Thank you. Glad you liked my lectures

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you did great! Learned a lot! thank you

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your videos are very helpful!

[@ericrugan007]

I really like your video and explanation. Except few things that you are repeating the same things again and again to prove the same point, which i feel too lengthy.

[@shomusbiologyofficial]

Thank you for reporting

[@anushamadireddy9844]

thank u Sir . very good explanation

[@fatemahaalhashem2308]

Excellent 👍👍 thank you so much

[@abebeyenesew4622]

it's nice but better if show it by 3D Animation. Deal on the main concept. Pictorial explanations are needed more for MSc Biomedical students.

[@wildcardtrader2671]

It would be great if you can reshoot the videos for better clarity

[@surendramishra9636]

Thank u Soo much sir.... For this video Nd please explain how can u prepair for CSIR

[@AnimeUni-versed]

You are the best !

[@shomusbiologyofficial]

You're welcome

[@aimansaleem6166]

What is the main difference between base excision and nucleotide excision repair??? I m confused in this.

[@shomusbiologyofficial]

Chcek by video on this difference

[@aimansaleem6166]

Thanks for replying.. What u told in the last of vedio just that is the difference,, I mean the difference of nucleotide, in base excision there is only one nucleotide to remove and in nucleotide, removal or 15 to 25 nucleotide?

[@speakup-24]

It's helpful ☺️

[@mridhusharma9907]

It is AP endocuclease that will only cleave off the back bone of the apurinic or apyrimidine sites, how can it chew the sequence that is not the AP site. Sir plz explain.

[@samuelscott8896]

Love this guy!

[@h.upekkhaabhayarathne2274]

Is this the direct repair mechanism of DNA? Need some clarification

[@shomusbiologyofficial]

What do you mean by direct repair?

[@h.upekkhaabhayarathne2274]

Photoreactivation of thymine dimer is an exmple fr tht i guess. Do you have a video on tht?? Please be kind enough to make one if not...........🙏🙏🙏🙏

[@zunairahbatool7573]

Thank you 💞

[@shomusbiologyofficial]

You're welcome

[@snehahalder7233]

Thank you 🙏

[@shomusbiologyofficial]

You're welcome

[@iglaniket1352]

The activity of dna poly 1 in ber for cleaving is 5' to 3' exonuclease not 3' to 5' ,other that great video 🙌🙌

[@iglaniket1352]

And for gap filling it's 5' to 3' polymerase activity

[@janardhan476]

Thank you sir it very good

[@shomusbiologyofficial]

You're welcome

[@mangobananana]

This is amazing!

[@shomusbiologyofficial]

+mangobananana thank you.

[@monababtain588]

life saver !! thanks

[@meetaajwani2642]

Is this for prokaryotes or eukaryotes? If anyone knows please help. Thank you

[@nneomaudeaja4522]

This is for prokaryotes

[@ashiqurrahman624]

Shomu What about deoxyribophosphodiesterase? It cleans up the backbone by removing a strech of neighboring sugar phosphate residues so that a DNA polymerase can fill the gap with nucleotides complementary to the other strands. Maybe this term is missing

[@khandaniyal7542]

Thank you so much

[@shomusbiologyofficial]

You're welcome

[@dipayandey327]

What about flap endonuclease ?

[@meghas3935]

can u mak a video about DNA damge and repair mechanism...pls

[@shomusbiologyofficial]

These are videos

[@meghas3935]

@@shomusbiologyofficial thank u

[@antaradas4320]

Thank you sir..

[@shomusbiologyofficial]

You're welcome

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Sir plz make a video on operon model in hindi

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Thnk u very much sir.

[@tulasibiswal2666]

Thanks sir 🙂

[@shomusbiologyofficial]

You're welcome

[@pkprabhakarjha]

Does any class I and class II AP endonuclease have any role here?

[@amenaakter3961]

Thank you

[@shomusbiologyofficial]

You're welcome

[@pratibha8953]

Can anyone tell me that this is proof reading mechanism of DNA repairs or not?? Plz reply fast anyone

[@shomusbiologyofficial]

Coming soon

[@pratibha8953]

@@shomusbiologyofficial thank you so much for this😇

[@LucyLovesRG]

great video but it's 5' to 3' exo

[@funworldzz]

3' to 5'

[@sathiyalakshmip260]

Yeah , It is 5' to 3'

[@manasasg4053]

Ur excellent

[@visalakshiboopathi9970]

Thanks

[@shomusbiologyofficial]

You're welcome

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Do micro biology video

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Thankyou so much :)

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Thanks.. that helped

[@shomusbiologyofficial]

+Aqsa Ashraf glad to hear that

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Tank you dear

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You're welcome

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Great

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[@alka-k3v]

2:35

[@sdfPZXC]

well explain sir XD ty a lot

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Nice

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Welcome

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very easy

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thanks sir

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+Anita Singh you're welcome

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Tnk u

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nyc

[@chaitra8473]

What is Nick sir

[@shomusbiologyofficial]

Phosphodiester backbone breakage

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👍

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👍👍💚

[@TheDuvee6]

Puma product placement detected

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sir language hindi me do please