TWiV 866: EV antibodies rEVolutionize our thinking

[@patrickkearney1577]

I like how Amy is always very precise in her discussions.

[@jmer9126]

I always could tell that Amy was brilliant and dedicated, but today I realized she is truly amazing.

[@SmallBizSpoken]

I have been a lurker since the pandemic interrupted life as we knew it. I have to comments on how radiant Amy looks with the new light. Good job! Thank you for your amazing work.

[@bid6413]

Thank you Amy especially for unveiling your work. Amazing and very interesting, even for a layman. I am amazed by the depth of your knowledge and creativity in research design. Cheers, Will

[@lesfaby8997]

Links for this episode 03:14 ABRCMS ePoster Spring Symposium for Emerging Scientists 05:42 Cross reactive enterovirus antibodies (mBio) 09:38 What is a serotype 24:54 Which polio serotype is dominant 26:01 These Viruses used to be categorized by infection route 31:40 Does species make sense with viruses 36:07 Cross reactivity makes it hard to distinguish viruses using serum Poliovirus receptor transgenic mice (virology blog) Timestamps by Jolene Thanks Weekly Picks 1:29:42 Dickson European scientists set new record in production of nuclear fusion energy 1:31:51 Amy Alfredo’s Magic Wand AFM is acute flaccid myelitis 1:35:47 Brianne What Happened After the Chicken Pox Vaccine 1:37:13 Rich Year Three of COVID-19 Harsh Truths Realities and Glimmers of Hope 1:39:05 Vincent Economists Are Fueling the War Against Public Health

[@Mark_Ocain]

Brianne has a knack for asking just the right questions.

[@patriciagiles5833]

Brianne didn't get where she is by being a schlub.

[@patriciagiles5833]

@@philipmccain5480 I saw a good interview she did recently that was in a zoom meeting format.

[@vtee361]

I love how they all got so excited at the end when were talking how important Amy’s work will potentially be. How can work with such ramifications not attract funding, surely this whole pandemic has taught us nothing but how important science is. The admiration these guys had for Amy’s work was palpable. Respect to all of you

[@jmer9126]

The light for Amy is a huge improvement. Thank you all for fabulous shows

[@celestekelsey696]

I found it too bright and harsh. I think Brianne's lighting is fine...could be a little "pinker" to soften but her light is even and not harsh. :)

[@fernie5128]

Amy! You are a gift and I wish I had all the money you needed...Thanks from Minnesota

[@BigGuy8059]

Does Amy's funding have to be from a grant? There are 108,000+ subscribers on this channel. If we all sent Amy 50 cents, she would have her 50K.

[@Mark_Ocain]

Amy lit up during this discussion... She was "In the zone"!!! Truly, she is the "Empress of Entero" LOL🤣 I could tell Dickson was enjoying the discussion as well.

[@norm1124]

Love the passion in this call 😍

[@stephanvonugron1944]

TWIV ALL STARS!! Great Episode!!!

[@animalparty8206]

👍👍👍👍👍Amy is a Science Goddess!!!!❤❤❤❤❤ So Brilliant!!! So Inspiring!!!! THANK YOU!!!😚

[@michelemurphy3541]

I absolutely love these podcasts. I lived in Austin for several years so seeing Rich Condit participating is super fantastic for me. The entire panel, all of you {!} are simply pure delight as I sit here, in my parent’s guest room, making jewelry or sewing up dog bow ties, while listening to these outstanding podcasts~ it makes my day very single time and I have a bazillion thanks to each and every one of you! Take care you guys and again, T H A N K YO U for all of the awesomeness-completely fantastic!!

[@noirleblanc00]

Great Episode! Amy’s work is so exciting.

[@katriel8693]

I have seen Brianne and Amy disagree before on TWIV. They are both so brilliant, I would love to see them collaborate! What a great episode

[@maryjanewhite5710]

This was a LOT OF FUN to watch!

[@cab5917]

Excellence in virology, research and science for the benefit of the world.

[@deborahfreedman333]

The best show so far, problem solving, experiment design and resource constraints, the fun stuff.

[@Bob_Huff]

Dickson was right: This was a great show. Gripping!

[@andreagradidge3752]

This episode is worth listening to twice (or more) for The Scientific Method. Brilliant!

[@Backtothescience]

Had to laugh when Amy recounted the story of Vincent saying you should respect my experience. I remember having exactly the same "discussion" with my supervisor when I was a PhD student. I was proven right as well.

[@sandsnowvancouver1]

Many thanks for another great podcast

[@miltz76]

Thanks TWiV! Another great one in the books.

[@gladysarmstrong889]

Great presentation so interesting. Thankyou👍

[@GIBKEL]

This is other level understanding. Just knowing what questions and how to ask them.-

[@marg716]

This. Is. FANTASTIC!!

[@Polkadotpup]

Thanks for posting the link to Laurie Garrett’s article.

[@Polkadotpup]

Vincent, you and Amy look great. What light do you use and where did you get it? Thanks

[@martinpollard8846]

good question, I'd like to know too

[@jballenger9240]

Me three.

[@lesfaby8997]

Great episode for understanding how science is done.

[@JasonCunliffe]

5:42 START

[@GaryLongsine]

"This is my favorite show of all time, right now." This is a defensible position.

[@2listening1]

4:20 Also, two names of the youngest sisters in Little Women. 👩🏼👩🏻

[@katriel8693]

Loved this episode

[@aaroncabral]

Amy, the venus of virology

[@norman_5623]

An aircraft carrier costs $13 billion. You could grow a lot of mice for that.

[@michaeladavis3]

Amy, to distinguish polio vs evd-68, can you do something similar to a colony in situ hybridization for one virus or the other? I used to have to do a colony lift southern to idententify rare recombination events when generating constructs for transgenics.

[@lisalisa20907]

Great episode!

[@jayakarjosephjohnson5662]

I think, Spectrometric study of Epitope molecules is imperative for the research on Précised vaccine development.

[@Polkadotpup]

Vincent…what type/brand of light do you use. You and Amy look great!

[@martinpollard8846]

good question, I'd like to know too

[@katriel8693]

Would also like to know

[@celestekelsey696]

"Science is a self-correcting discipline..." The truth will eventually emerge but in some cases do we have that kind of time. How many scientists died or worse before their discoveries were acknowledged? "Italian scientist Giordano Bruno was burned at the stake for teaching Copernicus’ heliocentric view of the Universe. Ignaz Semmelweis, Gregor Mendel, Gregor Mendel , and Ludwig Boltzmann 4 unfortunate scientists who made crucial discoveries but were dismissed during their time." The German physicist Max Planck said that science advances one funeral at a time.

[@erik_carter_art]

I was surprised by the expectation that individual short peptides comprising a larger conformational epitope will be bound by an antibody against that conformational epitope. I thought the reason antibodies with conformational epitopes work great for ELISA or histology or flow, but not for a Western blot, was because of this non-binding.

[@AMRosa10]

This is a fishing expedition, so all they need is a strong enough interaction for it to associate with the B-Cell/hybridoma receptor and have the two colocalize in a microfluidics well where it can be sequenced. What it sounds like is happening is that the barcoded peptides are still able to fold into a secondary structure with enough specificity which a portion of the antibody can bind weakly, which is enough for the peptide to tag the B-Cell for sequencing. The sequencing then gives you the full sequence of the immunoglobulin gene, which you would clone and transfect into new cells. You would then do binding assays to validate the affinity of binding. You don't need the type of affinity that you need for a blot, which has to hold on through washes. Also I am pretty sure that once you run and denaturing western you won't get refolding when you blot it, so there it should still just be in its primary structure, and an antibody wouldn't recognize that.

[@khhnator]

you would think that with modern technology, someone wold have tried to make a wiki where mistakes could get corrected quicker.

[@altyrrell3088]

Show Amy how much you care. Send her an EV isolate.

[@caygill2]

Excellent chapter!

[@cheeheifoo9282]

This stuff is difficult! Wow!

[@2listening1]

4:24 What is the “M” name, Amy? I think it’s cool to have four names, like a Royal!

[@jballenger9240]

What is the name of the light you mentioned Prof V.? And, take omega-3-fatty acids and vit D3, it’s good for us as our youth advances.

[@AbaseenPodcast]

LOL @ "Hydrogen Epitopes" 1:30:02 😂😂😂😂

[@claudia8861]

Amy's light is too light. It shines on her so much sometimes you don't see her nose. I hope she did not get a headache.

[@FewFishManyWorms]

Love the episode. Only thing I will say is I think you guys should bring a stuctural person on to discuss conformational epitopes. I think Amy's (and Brianne's follow up) is completely wrong. Linear peptides work for linear epitopes. Short peptides almost never even recapitulate linear conformational epitopes (such as a singular loop). Putting multiple small peptides together also will not recapitulate a conformational epitope. What CAN happen is that if an antibody binds a conformational epitope that is largely linear, it can still have enough affinity to bind. But look at epitope-focussd immunogen design approaches such as those around AS412 of HCV. Peptides do not recapitulate the necessary spatial arrangement (tertiary and quaternary structure) for binding of actual conformational epitopes. I really hope it works for you, but I just completely disagree with the rationale.

[@noreenquinn3844]

Wow!

[@safimoshkani8495]

I didn’t know that human T cells if exist in serum are able to generate immune response in mice upon transfer of immune serum

[@eottoe2001]

The University of Louisville medical school may not like the descriptions of its programs from the 1890s. LOL

[@ScotHarkins]

Amy keeps her house because she knows she's right and today showing she's right will be almost trivial...if the people reading the grant app have the wit to see she's coming forearmed, or will in the next version of the application.

[@ScotHarkins]

Dr Oliver of "Back to the Science" did a nice video takedown of the shutdown "study" (really it's just a paper). "Lockdown Slapdown? 'Meta-analysis' that ignores most of the literature". Just wait until she gets to the weighting...oh dear.

[@luismatheu4226]

Antibody mapping

[@missano3856]

Yay, first comment.

[@martinpollard8846]

lol

[@ruthmcbride1778]

What is the. Canyon? I bot lost

[@AMRosa10]

It is a part of the structure of the capsid of enteroviruses. If you think of a soccer ball, but exaggerate it so that the faces really stick up and the seams are really sunken, the place where the seams are would be about where the canyon is on a viral capsid like that of poliovirus. You wouldn't expect that an antibody can squeeze into that space, however there are data to show that antibodies can actually bind this area of the viral capsid.

[@animalparty8206]

Thanks AMRosa! I was going to attempt to explain this but would never have been able to do it so eloquently!!

[@christopherrobinson7541]

Amy, how much money do you need?

[@peterbooker3908]

Stick to what you know you'll have a better show!

[@skepticalbadger]

This is literally about one of their pieces of research. They "know". Do you? All you post is empty criticism.

[@malcolmmarten9865]

If you live long enough you may realize the importance of this work presented and will have had the privilege of seeing the author present it. A rare opportunity. I thought the show was magnificent.

[@BigGuy8059]

Does Amy's funding have to be from a grant? There are over 108,000 subscribers on this channel. If we all sent Amy 50 cents, she would have her 50,000.

[@toby9999]

Good question. Nice idea if possible.