What are restriction enzymes

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Sir whole graduation is completeed bkz of u. Ur 10 mints videos nade my life. Sir alhamdillah. I m the silver medalist of my institute.. And u played a major role for my this achvmnt..my ever best tchr.. Love u frm core of my heart. Need ur comnt below.. ♥️😍 Plz sir. I will uploaded. S. S on my social accounts..

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Hi Suman bro ... ! Krishna here from Bombay in 3rd yr bsc biotech. What an Awesome video bro.. !Expained it so wonderfully, slowly and clearly. Cannot expect a better explanation buddy.Great work.Nice of you to share the knowledge..help others.. Respect for u Mahn .. Thumbs up :).. I along wid friends Followd allmost all ur videos for the exams..as Lecs in college don't help much due to hapazard incomplete and careless teaching. :/ understood fully and better after seeing ur videos. U will be respected every where bro.. Thanks..Owe u for ur efforts ..Cheers from Bombay..

[@shomusbiologyofficial]

Thank You friend. I am glad that my videos are helping you guys. Stay tuned. There are more to come. All the best.

[@lalithsasubilli8103]

damn! im in 9th grade and i have to learn this crap!

[@rajbaghouse1657]

"Hey! This is part of your cell don't cleave me " 😂😂 however sir you are awesome.. I am in BSC biotechnology 3rd year... And I started watching your video from f.y. BSC... At every exam at end of paper I write "credits goes to suman sir from sohmus biology"

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Extremely useful for me! I am going to recommend it..thank you Shomu.

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Thanks sir, thank u so much! It really helped me clearing the doubts which were termed as "too inquisitive" by my teacher as we were being taught from NCERT but, I really like these topics..and u know how good for nothing is NCERT!

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you have a way of making concepts easier, thank you.

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@@protivasaha2575 hmm thik bolechen....bhalo teacher ...but totlay......

[@kunbidia1470]

thanks for the lecture great one but what i know is EcoR1 cleaves G A not A T, it is Sma1 that cleaves A T creating blunt ends while the latter creates sticky ends

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[@MultiDatta]

Thanks brother !! Saved my genetics lab preparation. Hats off

[@paulingraham6781]

I thought I might make a comment for all those who are like me and don't watch videos that much the whole way through in general, this one about endonucleases: an endonuclease is an enzyme which cleaves a chain of linear polymers comprised of nucleotide units bonded together (polynucleotides) by separating nucleotides except for those on the periphery.

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Sir msc biotechnology k bad kya future options available hai?

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you are so good in explaining things. i always watch your videos

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[@srividya4602]

@Shomu's Biology Sir what if the restriction enzymes created by bacteria are Isoschizomers? (Isoschizomers can recognise n cleave methylated dna sequences)

[@Gmhi6615]

well i want to understand this specific topic bcz my base is very weak in Biotechnology...you did it just fine... thanks bro

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You deserve much more recognition ❤

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@@shomusbiologyofficial The fact that u are still replying to comments even after 7-8 years of upload is amazing. You definitely are in love with the subject and it clearly reflects in your way of teaching, how you explain everything with so much ease and interest. THANK YOU so much sir for your valuable lectures, it hepls us students in ways one can not fully explain. Best wishes for u may god give u happiness, prosperity and good health.

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U r d life saviour of students like us

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great video!!!!!!.thank you sir.i have been preparing for NTS exam and I am sure that this will definitely help me.thanks alot once again

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Hello Suman sir.. Your videos are quite clearly understood able and most helpful and easy to memorize. I really appreciate your handwork and would like you to make more such videos for easy understanding about biology for the students.. I would like to you to make one video on how to upload the Genomic Sequences at NCBI. Thank you.

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Thank you. I am planing to make whole new Bioinformatics video series soon

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@@shomusbiologyofficial Thank you sir will be waiting.

[@harikrishna7036]

Sir.Very nice.but I have one doubt.how restriction endonuclease enzyme do not affect our parental genome.?.You say there is methylase in parental genome..But phage genome also have methylase ..But how is specifically affect the phage genome not bacterial genome.i am really confused that area?Can u pls explain?

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Honestly you are an excellent guy...I know how the DNA molecule becomes straight after a while..... seriously you guys are amazing teachers....I saw a few KZbinrs and you're great and I am sure you can only have this kind of commentaries.... I love scientist that post their notes....I believe we can see always another angle from somebody else and I like that.....and always an enriching one too.

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You explain everything so beautifully. .always make the complicated stuffs simplified and easily understandable. .thank you👍

[@saicharitha1121]

Thankuu broo for explaining ....and I think it would be still more useful for competitive exams if u explain the nomenclature of some more enzymes... Plz please try to upload one more video for explaining some more examples so that it will be useful a lot... Even me and my friends almost many of us listen to ur classes thanks bro for explaining so clearlyyyy....

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[@kalpanakulshreshtha2727]

Very good explanation.

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Wonderful session sir 👍👍

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[@sushenasxr]

sir i hav exam exams tomo so could u pls make a video on restriction mapping

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I'm from Egypt and follow you. I understand from you and appreciate your efforts ,Thanks .👏💜

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Superb.... Sir... This video cleared my doubt. Tnq sir

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[@wesammagdy2018]

i want to know according to what the enzyme has its specific seq. of nucleotides which it can recognize it ?

[@chainikagupta5360]

sir..need video on pcr troubleshooting

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good n digestible lecture

[@raveenabiswas3333]

you dont be thank for watching ...im thanking you for explaining

[@BaBaN7777]

Sir from where the restriction enzymes were formed inside the bacterial cell? Which organelle responsible for this?

[@WakgariAsfew]

thank u so much, i learned more about restriction enzymes

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[@kenectic]

Even if it has like a written code for a recognition signal sequence. How do restriction enzyme know how to do its job? How does it know where to go?

[@A1Kalid]

+kenectic it doesn't GO anywhere , its everywhere , the DNA produce so much it fells the hall place of them , swimming everywhere , .

[@abhijitbarman3908]

hii ,sir R.E is only the restriction endonuclease?? nothng more?? can't be othr nucleases lyk exo? or res.endonuclease??

[@wesammagdy2018]

there are other endonucleases ,but R.E are the most common.

[@ananthjai2592]

Exo is not cut a correct cleavage it will cut diffrent area so these no use in R.E

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Happy techers day sirr. Sir plz held your Live session at any day. Love from pakistan.

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Hyy sir aap rdt kis book s padha rhe plz comment sir

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Didn't recall now

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Amazingly done... Just awesome thank you sooo much sir. Allah bless you. Love and respect from Pakistan. You are my ideal teacher. 👌👌👌👌👌👌👌👌

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Sir I just love your class..! It's very much understanding and interesting

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thank you so much, it realy influenced by ur way of teaching. it is simple but really effective

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Best explanation sir.. I lv ur vdo

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Hats off to u brdr😊....god bless u always👌😊😊👍👍

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[@biologybykomal8248]

In nomenclature Roman numeral tells about order of isolation

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Thank you so much sir... ❤❤

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thanks alott for ur all videos... they helped alott..

[@bishobkh1935]

Sir what about the phage genome it would not function anymore??

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Yes you are doing alot alot for others

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very good video sir .. i am fan of you

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[@DELMohdHasanKhan]

Sir ...bacterial genome produce R.E or phage produce R.E.....i m confused little bit

[@KHUSHBOOKUMARI-hw4zm]

Just awesome ... thanks a lot sir

[@shomusbiologyofficial]

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[@hinawajid1575]

Thanks a lot for a remarkable lec. I've a qs: If the restriction site sequence is same for bacteria n phage to be cleaved, How does the bacteria recognize that its his own DNA to be modified or its bacteriophage's? Can't it modify phage's DNA? Kindly answer. Thank You

[@shomusbiologyofficial]

Bacteria protects its own DNA by methylation it

[@chandanlalkarn8]

Really good tutorial

[@M_Alatawi]

Many thanks. It is very helpful.

[@shomusbiologyofficial]

Glad it helped

[@manu3989]

Dear Shomu, I really like your teaching style. you are doing great work. But, in this video, I have not convinced on the content you said from 8.26 to 9.20?? could you please clarify? As I know, it is the bacteria which produces REs to kill invading foreign DNA not the vice versa! Thanks Mohan

[@kaustavsengupta8757]

Mohan TC From that what he meant that virus DNA do have some protein that help viral DNA to get cut and paste itself inside the bacterial DNA so that virus can hijack host, and start it's own replication. Since it's all the game of probability, whether protien enzymes(DNA of Virus) is able to find appropriate location to get inside before host immune system kills it or not. That's the reason why we use term "plague formation unit" amount of virus needed to infect a cell, incase of influenza (common cold) it need around 350 to infect a cell whereas Polio virus is nearly upto 40-50.

[@anshulrana6211]

Sir can you make another video of this

[@shomusbiologyofficial]

New version coming soon

[@anshulrana6211]

@@shomusbiologyofficial thanks ❤️❤️❤️

[@subhashreesahoo4589]

Excellent vdo sir🙂

[@pikkukumari6965]

sir cutting pattern of enzymes plz send link

[@devilgodking2666]

I have'nt understand the R-1 in e.colli and how we get the R-1 please explain me sir

[@devilgodking2666]

There is no-one to give the answer

[@aditirattan8190]

@@devilgodking2666 😂😂

[@Universe_41]

R-1 where R means rough and 1 tells the order of in which this restriction enzyme was obtained may be this means that first restriction enzyme obtained in ecoli was EcoR1 hence 1 is written there

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thanks man. that was very helpful!!

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Thanku sir... God bless you always 😇💓🌼🤗🙏

[@shomusbiologyofficial]

You're welcome

[@rosefinch3759]

Great video! Very helpful!

[@rishideepbhagat988]

Sir - please make video on enzyme technology

[@preetisharma-mi8fk]

Sir please make a video for codon optimization

[@shomusbiologyofficial]

Will

[@kiara1231971]

I have a question in what year of university do you study restriction enzymes?

[@shomusbiologyofficial]

Bsc 3rd year

[@vaishalithukral9129]

Sir what if phage dna doesnt contain recognition sequence for restriction endonuclease enzyme?

[@pukhrajmali5972]

Bacteria produce RE which is specific for phage

[@elizabethnx]

That phage can infect bacteria..RE is protecting bacteria from phage

[@anjalikhan4232]

why Restriction site is added to primer.....

[@arnitheking]

very nice vid, great explanation

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thank you so much sir👏👏👏👏

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Thank you so much sir❤️

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Great sir g...! Impressive

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[@thiwankaperera8603]

Thank you so much..clearly exaplained 👍👍👍

[@shomusbiologyofficial]

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[@missbal0ch829]

Are Restriction enzymes responsible for the breakage of both hydrogen bonds b/w N.B and also phosphodiester bond b/w the sugar to form the sticky ends of DNA???? please sir try to solve my confusion

[@pukhrajmali5972]

RE cut phosphodiester bond

[@PoojaSaini-cz5fu]

Thank you so much 🙏😄

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@nnandini7566]

Sir please make a vedio of poly nucleotide Kinase

[@ashdeep5991]

is there any difference between restriction enzyme and endonuclease?

[@shomusbiologyofficial]

Yes

[@ashdeep5991]

what? Restriction enzyme or restriction endonuclease make cleaves at specific point whereas endonuclease doesn't do the same, is it the main difference?

[@jyotisgarma]

Thank you so much sir ❤ this makes me very heplful . God bless you 🥰

[@shomusbiologyofficial]

You're welcome. Glad you like it

[@ashutoshs1966]

Correction...The Roman number in the end denotes order of identification

[@faithagbede5076]

Well done am greatfull for this video, I must say I comprehend easily💯

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[@Lily-cl6zk]

So clear! Thank you 🙏🏻

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🙏no words just hats off💯

[@Anonymous-n8i2d]

That was a great video. thank you

[@MK-wr5ki]

very good awesome lec bro........EXPLAIN ME 3 TO 5 AND 5 TO 3 SEQUNCE IN DNA PLEASE n

[@tejasbadhe6199]

M K please learn first spelling of"sequence" befaor taking a big leap to restriction enzymes

[@sidkfunday8454]

Tejas u also have to learn the spelling of before firstly🤣🤣

[@allsops3062]

🙏🤗Thank you for sharing this knowledge ❤️ very gifted and humble teacher. You really teach so I really learn.🤗🙏

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Dhanyavad

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Great sir! 👌👍

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