What are restriction enzymes

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Hi Suman bro ... ! Krishna here from Bombay in 3rd yr bsc biotech. What an Awesome video bro.. !Expained it so wonderfully, slowly and clearly. Cannot expect a better explanation buddy.Great work.Nice of you to share the knowledge..help others.. Respect for u Mahn .. Thumbs up :).. I along wid friends Followd allmost all ur videos for the exams..as Lecs in college don't help much due to hapazard incomplete and careless teaching. :/ understood fully and better after seeing ur videos. U will be respected every where bro.. Thanks..Owe u for ur efforts ..Cheers from Bombay..

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damn! im in 9th grade and i have to learn this crap!

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‘A bacterium’* Thanks for the detailed explanation. You’re the best!

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"Hey! This is part of your cell don't cleave me " 😂😂 however sir you are awesome.. I am in BSC biotechnology 3rd year... And I started watching your video from f.y. BSC... At every exam at end of paper I write "credits goes to suman sir from sohmus biology"

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Sir msc biotechnology k bad kya future options available hai?

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well i want to understand this specific topic bcz my base is very weak in Biotechnology...you did it just fine... thanks bro

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you have a way of making concepts easier, thank you.

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great video!!!!!!.thank you sir.i have been preparing for NTS exam and I am sure that this will definitely help me.thanks alot once again

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[@kunbidia1470]

thanks for the lecture great one but what i know is EcoR1 cleaves G A not A T, it is Sma1 that cleaves A T creating blunt ends while the latter creates sticky ends

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@@protivasaha2575 hmm thik bolechen....bhalo teacher ...but totlay......

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you are so good in explaining things. i always watch your videos

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Honestly you are an excellent guy...I know how the DNA molecule becomes straight after a while..... seriously you guys are amazing teachers....I saw a few KZbinrs and you're great and I am sure you can only have this kind of commentaries.... I love scientist that post their notes....I believe we can see always another angle from somebody else and I like that.....and always an enriching one too.

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[@paulingraham6781]

I thought I might make a comment for all those who are like me and don't watch videos that much the whole way through in general, this one about endonucleases: an endonuclease is an enzyme which cleaves a chain of linear polymers comprised of nucleotide units bonded together (polynucleotides) by separating nucleotides except for those on the periphery.

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Hello Suman sir.. Your videos are quite clearly understood able and most helpful and easy to memorize. I really appreciate your handwork and would like you to make more such videos for easy understanding about biology for the students.. I would like to you to make one video on how to upload the Genomic Sequences at NCBI. Thank you.

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Thank you. I am planing to make whole new Bioinformatics video series soon

[@sheikhgazala205]

@@shomusbiologyofficial Thank you sir will be waiting.

[@harikrishna7036]

Sir.Very nice.but I have one doubt.how restriction endonuclease enzyme do not affect our parental genome.?.You say there is methylase in parental genome..But phage genome also have methylase ..But how is specifically affect the phage genome not bacterial genome.i am really confused that area?Can u pls explain?

[@BaBaN7777]

Sir from where the restriction enzymes were formed inside the bacterial cell? Which organelle responsible for this?

[@srividya4602]

@Shomu's Biology Sir what if the restriction enzymes created by bacteria are Isoschizomers? (Isoschizomers can recognise n cleave methylated dna sequences)

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[@kalpanakulshreshtha2727]

Very good explanation.

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Thankuu broo for explaining ....and I think it would be still more useful for competitive exams if u explain the nomenclature of some more enzymes... Plz please try to upload one more video for explaining some more examples so that it will be useful a lot... Even me and my friends almost many of us listen to ur classes thanks bro for explaining so clearlyyyy....

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@@shomusbiologyofficial The fact that u are still replying to comments even after 7-8 years of upload is amazing. You definitely are in love with the subject and it clearly reflects in your way of teaching, how you explain everything with so much ease and interest. THANK YOU so much sir for your valuable lectures, it hepls us students in ways one can not fully explain. Best wishes for u may god give u happiness, prosperity and good health.

[@DELMohdHasanKhan]

Sir ...bacterial genome produce R.E or phage produce R.E.....i m confused little bit

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good n digestible lecture

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Amazingly done... Just awesome thank you sooo much sir. Allah bless you. Love and respect from Pakistan. You are my ideal teacher. 👌👌👌👌👌👌👌👌

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[@hinawajid1575]

Thanks a lot for a remarkable lec. I've a qs: If the restriction site sequence is same for bacteria n phage to be cleaved, How does the bacteria recognize that its his own DNA to be modified or its bacteriophage's? Can't it modify phage's DNA? Kindly answer. Thank You

[@shomusbiologyofficial]

Bacteria protects its own DNA by methylation it

[@WakgariAsfew]

thank u so much, i learned more about restriction enzymes

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you dont be thank for watching ...im thanking you for explaining

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Superb.... Sir... This video cleared my doubt. Tnq sir

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thanks alott for ur all videos... they helped alott..

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Hyy sir aap rdt kis book s padha rhe plz comment sir

[@shomusbiologyofficial]

Didn't recall now

[@sushenasxr]

sir i hav exam exams tomo so could u pls make a video on restriction mapping

[@abhijitbarman3908]

hii ,sir R.E is only the restriction endonuclease?? nothng more?? can't be othr nucleases lyk exo? or res.endonuclease??

[@wesammagdy2018]

there are other endonucleases ,but R.E are the most common.

[@ananthjai2592]

Exo is not cut a correct cleavage it will cut diffrent area so these no use in R.E

[@allsops3062]

🙏🤗Thank you for sharing this knowledge ❤️ very gifted and humble teacher. You really teach so I really learn.🤗🙏

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[@kiara1231971]

I have a question in what year of university do you study restriction enzymes?

[@shomusbiologyofficial]

Bsc 3rd year

[@biologybykomal8248]

In nomenclature Roman numeral tells about order of isolation

[@missbal0ch829]

Are Restriction enzymes responsible for the breakage of both hydrogen bonds b/w N.B and also phosphodiester bond b/w the sugar to form the sticky ends of DNA???? please sir try to solve my confusion

[@pukhrajmali5972]

RE cut phosphodiester bond

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Really good tutorial

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sir..need video on pcr troubleshooting

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Hats off to u brdr😊....god bless u always👌😊😊👍👍

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Wonderful session sir 👍👍

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Thank you so much sir ❤ this makes me very heplful . God bless you 🥰

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Many thanks. It is very helpful.

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Glad it helped

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Yes you are doing alot alot for others

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Well done am greatfull for this video, I must say I comprehend easily💯

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Sir - please make video on enzyme technology

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Happy techers day sirr. Sir plz held your Live session at any day. Love from pakistan.

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Best explanation sir.. I lv ur vdo

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Thank you so much sir... ❤❤

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Thanku sir... God bless you always 😇💓🌼🤗🙏

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[@duaam3100]

thanks man. that was very helpful!!

[@wesammagdy2018]

i want to know according to what the enzyme has its specific seq. of nucleotides which it can recognize it ?

[@kenectic]

Even if it has like a written code for a recognition signal sequence. How do restriction enzyme know how to do its job? How does it know where to go?

[@A1Kalid]

+kenectic it doesn't GO anywhere , its everywhere , the DNA produce so much it fells the hall place of them , swimming everywhere , .

[@rosefinch3759]

Great video! Very helpful!

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Just awesome ... thanks a lot sir

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Thank you so much..clearly exaplained 👍👍👍

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[@manu3989]

Dear Shomu, I really like your teaching style. you are doing great work. But, in this video, I have not convinced on the content you said from 8.26 to 9.20?? could you please clarify? As I know, it is the bacteria which produces REs to kill invading foreign DNA not the vice versa! Thanks Mohan

[@kaustavsengupta8757]

Mohan TC From that what he meant that virus DNA do have some protein that help viral DNA to get cut and paste itself inside the bacterial DNA so that virus can hijack host, and start it's own replication. Since it's all the game of probability, whether protien enzymes(DNA of Virus) is able to find appropriate location to get inside before host immune system kills it or not. That's the reason why we use term "plague formation unit" amount of virus needed to infect a cell, incase of influenza (common cold) it need around 350 to infect a cell whereas Polio virus is nearly upto 40-50.

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Excellent vdo sir🙂

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very good video sir .. i am fan of you

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[@arnitheking]

very nice vid, great explanation

[@ganeshmunikuntla5125]

Sir who cut the hydrogen bonds during the activity of endonuclease

[@anshulrana6211]

Sir can you make another video of this

[@shomusbiologyofficial]

New version coming soon

[@anshulrana6211]

@@shomusbiologyofficial thanks ❤️❤️❤️

[@pikkukumari6965]

sir cutting pattern of enzymes plz send link

[@Anonymous-n8i2d]

That was a great video. thank you

[@devilgodking2666]

I have'nt understand the R-1 in e.colli and how we get the R-1 please explain me sir

[@devilgodking2666]

There is no-one to give the answer

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@@devilgodking2666 😂😂

[@Universe_41]

R-1 where R means rough and 1 tells the order of in which this restriction enzyme was obtained may be this means that first restriction enzyme obtained in ecoli was EcoR1 hence 1 is written there

[@bishobkh1935]

Sir what about the phage genome it would not function anymore??

[@dinujaperera5526]

Thank you so much sir❤️

[@shomusbiologyofficial]

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[@nnandini7566]

Sir please make a vedio of poly nucleotide Kinase

[@sadamlakhvii1667]

Great sir g...! Impressive

[@shomusbiologyofficial]

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[@vaishalithukral9129]

Sir what if phage dna doesnt contain recognition sequence for restriction endonuclease enzyme?

[@pukhrajmali5972]

Bacteria produce RE which is specific for phage

[@elizabethnx]

That phage can infect bacteria..RE is protecting bacteria from phage

[@waltermood8827]

Very Interesting

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Thank you

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Sir please make a video for codon optimization

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Will

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Thank you so much 🙏😄

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thank you so much sir👏👏👏👏

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Dhanyavad

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So clear! Thank you 🙏🏻

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[@dr.jogendrasinghnim4219]

there great videos helpful to all

[@dhruvk7892]

explained well

[@ashdeep5991]

is there any difference between restriction enzyme and endonuclease?

[@shomusbiologyofficial]

Yes

[@ashdeep5991]

what? Restriction enzyme or restriction endonuclease make cleaves at specific point whereas endonuclease doesn't do the same, is it the main difference?

[@helyrbhatiya]

🙏no words just hats off💯