Yeast two hybrid system

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Shomu's Biology

Shomu's Biology

Күн бұрын

Пікірлер: 80
@MrLister97
@MrLister97 5 жыл бұрын
Always when I search some biological methods in youtube I hope that there will be a Shomu's video...and it is always there!! Thank you man! I will mention you during my graduation ceremony!
@shomusbiologyofficial
@shomusbiologyofficial 5 жыл бұрын
Glad to hear that you're getting benefit from my lectures
@akikonasaki
@akikonasaki 9 жыл бұрын
You are saving me in times of darkness (finals are coming) Luv you bro
@shomusbiologyofficial
@shomusbiologyofficial 9 жыл бұрын
+akikonasaki Thank you
@nunitchagucci3047
@nunitchagucci3047 7 жыл бұрын
I don't know why some people complain about this video. I find it is amazing and very helpful. I'm undergraduate and my research project used Y2H for the experiment. After watching this video I understand the concept of this Y2H completely. I like how you explained that the Y2H is like making a "check point" by using the yeast organism as a demonstration. I usually don't write a comment but you should give a thump up bro! For those who don't understand your language and accent, I find it's helpful to turn on the CC, pause the video and write down what he said. Some science concept are really complicated and you need to paused and break down each points. There are a lot of information in this video so it's not just really his fault about explaining things. It's about your efforts trying to learn it from it as well. Anyway, your channel is truly amazing bro! :))
@Agonymous
@Agonymous 11 жыл бұрын
Textbook is Bruce Albert Molecular Biology of the Cell chapter 8.
@yusun4390
@yusun4390 5 жыл бұрын
Where can we find it on the sixth edition?
@KinGamerBoy568
@KinGamerBoy568 3 жыл бұрын
This man is at the top of his game🙌🏼
@shomusbiologyofficial
@shomusbiologyofficial 3 жыл бұрын
Thank e
@parthabiswas5212
@parthabiswas5212 5 жыл бұрын
Your videos are really informative.
@shomusbiologyofficial
@shomusbiologyofficial 5 жыл бұрын
Thank you
@burnit_0193
@burnit_0193 11 жыл бұрын
Very well explained. Thank you sir.
@Sarah-oc2oi
@Sarah-oc2oi 6 жыл бұрын
On ya Shomu! Great work
@shomusbiologyofficial
@shomusbiologyofficial 6 жыл бұрын
You're welcome
@sheenashaikh5534
@sheenashaikh5534 7 жыл бұрын
Seriously ur videos are very helpful for me as i am a biochemistry student and am very thankful to u My concepts gets more clear after watching ur videos 😃😃
@wasimkhanlive
@wasimkhanlive 3 жыл бұрын
What is the name of this book and sir need it please?
@educationinfo5080
@educationinfo5080 Жыл бұрын
Thank you sir❤
@shomusbiologyofficial
@shomusbiologyofficial Жыл бұрын
You're welcome
@j2zel
@j2zel 4 жыл бұрын
Why would someone use this technique instead of just doing a simple Co-IP to see if the two proteins interact?
@darulizakernain7758
@darulizakernain7758 11 жыл бұрын
thank you simple explanation easy to understand
@julianahabeeb9881
@julianahabeeb9881 8 жыл бұрын
thank you ... great video
@shomusbiologyofficial
@shomusbiologyofficial 8 жыл бұрын
+JULIANA HABEEB glad it helped
@13loodLust
@13loodLust 11 жыл бұрын
what etextbook is that?
@TimothyOFlynn
@TimothyOFlynn 11 жыл бұрын
How would you know which bait and prey proteins to start with? For example if you had the blue crescent one as bait and another prey protein that doesn't fit, they won't bind to the transcriptional activator binding site, therefore no transcription nor translation. But does that then mean you need to plate a Petri dish for every possible protein in the cell? e.g. plate #1 has bait as blue crescent and prey as a purple bulb, plate #2 has bait as blue crescent and prey as a red bulb etc etc
@j2zel
@j2zel 4 жыл бұрын
Thanks for the clear video. What textbook do you get your figures from?
@unclehelicopter
@unclehelicopter 8 жыл бұрын
Great video! I understood that this method is usually done in a matrix of thousands of proteins X thousands of proteins. After we get the interaction pattern, how can we trace which are the exact interacting proteins?
@rasmasyean
@rasmasyean 11 жыл бұрын
Does this always work? How can you tell if the orientation of the bait and prey attached to the BD and AD will make it start translation? If you attach it "wrong", wouldn't a dimer be so misformed that it wouldn't translate regardless of a successful bind?
@guitarmanuseless
@guitarmanuseless 10 жыл бұрын
Really nice explanation....Thank you
@danielho4181
@danielho4181 10 жыл бұрын
But otherwise, your videos are really good. Thanks:)
@aboutstudies123
@aboutstudies123 7 жыл бұрын
Thank you Sir. It is very helpful. I have a small query Sir: Generally, after Transcription the m-RNA is produced, but in Yeast the reporter protein is produced?
@ektaverma8398
@ektaverma8398 4 жыл бұрын
Which book photo is dis?
@sarahfernandez3515
@sarahfernandez3515 11 жыл бұрын
Thanks this was very helpful
@nkululekokhumalo6332
@nkululekokhumalo6332 11 жыл бұрын
thank you sir . very helpful
@srikantadash4603
@srikantadash4603 4 жыл бұрын
Amazing video so helpful ❤😇 Can u plse say which book is this suman sir?
@shomusbiologyofficial
@shomusbiologyofficial 4 жыл бұрын
Didn't recall
@AnthonyChanthony
@AnthonyChanthony 12 жыл бұрын
Amazing, concise explanation! Thank you =]
@stijnvanmegen1032
@stijnvanmegen1032 7 жыл бұрын
can you tell me something about, out of frame or antisense sequence clones ? especially how they arise and why to discard them
@13loodLust
@13loodLust 11 жыл бұрын
this is awesome. Thank you!
@hernolepooja
@hernolepooja 12 жыл бұрын
informative video. thank u.
@bamibalboy
@bamibalboy 8 жыл бұрын
very helpfull, thank you!
@jamesmallon1631
@jamesmallon1631 6 жыл бұрын
What is stopping the transactivating domain protein (prey) binding the bait if the target protein and partner do not bind? There must be a system in place to stop all the false positive readings, can someone explain that to me?
@AxisLearning
@AxisLearning 11 жыл бұрын
Many thanks!
@kapilr4002
@kapilr4002 10 жыл бұрын
nice ! only that i find the explanation quite slow
@souuuful
@souuuful 12 жыл бұрын
good work
@木南-x3w
@木南-x3w 5 жыл бұрын
thank you~
@shomusbiologyofficial
@shomusbiologyofficial 5 жыл бұрын
You're welcome
@The91Walrus
@The91Walrus 12 жыл бұрын
I loved that video, but why is there that high rate of false positives?
@OptimisticCard
@OptimisticCard 11 жыл бұрын
Many thanks
@thisiseverything2
@thisiseverything2 10 жыл бұрын
Thank you
@chua41
@chua41 7 жыл бұрын
if you could explain false (+) and false (-)
@rupammajumder2764
@rupammajumder2764 5 жыл бұрын
Shomu da ei prothom tomr video ...ta kono kaje laglo na..tui Yeast cell mating er upore ekta ....series banao na
@jasminekovvada3626
@jasminekovvada3626 7 жыл бұрын
excellent
@shomusbiologyofficial
@shomusbiologyofficial 7 жыл бұрын
+Jasmine Kovvada thank you.
@patriciapt
@patriciapt 10 жыл бұрын
thankyou!
@rubykayyy777
@rubykayyy777 7 жыл бұрын
LOVEDDDDD IT
@pedromesquita324
@pedromesquita324 6 жыл бұрын
Which book did you take the image from?
@pushpasharma1813
@pushpasharma1813 6 жыл бұрын
Cell by Bruce Alberts
@Obsto123
@Obsto123 8 жыл бұрын
Thank You :D
@kattavia92dva
@kattavia92dva 9 жыл бұрын
Why the false positives though???
@AnkushSharma-zv5hv
@AnkushSharma-zv5hv 7 жыл бұрын
thanx..........
@sylwyu1
@sylwyu1 7 жыл бұрын
cheers mate! Like!
@chandnivora1109
@chandnivora1109 2 жыл бұрын
should be in more detail. not clearly understood
@jayalalkj1576
@jayalalkj1576 6 жыл бұрын
This is not fully writing .
@jessicafuhriman681
@jessicafuhriman681 11 жыл бұрын
And this is also a better video if you didn't understand the one above: sumanasinc. com/ webcontent/animations/content/yeasttwohybrid.html
@satishsasikumar
@satishsasikumar 8 жыл бұрын
Shomu, I am sorry to say that both your explanation of yeast two-hybrid (y2h) system and your language is very poor. Initially, one has to begin describing in a very simple way explaining what it is, how and why it was developed, elucidate the PRINCIPLE of y2h system elaborating on each component of the assay separately, explain the methodology of reporter assay and later describe how it is useful and different from other assays etc. I have used this assay many times in my work and I did not find your explanation coherent, logically framed step- by- step that could be useful for students who do not even know what y2H is!
@fredapatel3510
@fredapatel3510 7 жыл бұрын
Ok then genius show us how to do it......... waiting.........
@1Drocksthehouse
@1Drocksthehouse 6 жыл бұрын
ok sorry this is on par and on target and hit every important point ok first u try come explain ah sial i taking science in college now and he made me get an A ok dont talk cock
@peterkuzmin8624
@peterkuzmin8624 9 жыл бұрын
Your accent is killing me. I can't watch this. I'm sorry.
@DrReginaldFinleySr
@DrReginaldFinleySr 9 жыл бұрын
+Peter Kuzmin You are a rude idiot Peter. Not even necessary.
@peterkuzmin8624
@peterkuzmin8624 9 жыл бұрын
+Reginald Finley Well if all you get is positive reviews then there is nothing to improve. Now he knows what to improve to be a better lecturer. That was a rude comment and I probably should have worded it differently.
@DrReginaldFinleySr
@DrReginaldFinleySr 9 жыл бұрын
So you want him to "fix" his accent?
@peterkuzmin8624
@peterkuzmin8624 9 жыл бұрын
+Reginald Finley yes, just like I did when I learned English because it wasn't my first language. The reality is that a lot of people have trouble understanding foreign accents. Fixing your accent to be more articulative is just more professional. Especially when your job involves lecturing. If you have ever been to a few university lectures you know what I'm talking about. And yes, maybe it's not completely PC to say this but it's the truth.
@chanwoo1143
@chanwoo1143 9 жыл бұрын
+Peter Kuzmin dont know about his accent. I cant understand his english man
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