Agarose Gel Electrophoresis

For more information, visit www.bio-rad.com....
This video demonstrates how to load and run DNA samples on an agarose gel. Basic information about the charge of DNA and how it will run in an horizontal electrophoresis cell is explained.
www.bio-rad.com...
We Are Bio-Rad Explorer.
Our Mission: Bio-Rad’s Explorer program provides easy access to engaging hands-on science learning experiences that spark interest in science and its influence on the world.
To do this we:
Provide high quality, relevant, learning experiences based on real world science
Empower educators with skills and confidence to deliver engaging, memorable lessons
Connect with Bio-Rad Explorer Online:
Website: www.bio-rad.com...
Twitter: / bioradeducation
Pinterest: / bioradexplorer
LinkedIn: / 1613226
Facebook: / biorad
Instagram: @BioRadLabs
Snapchat: @BioRadLabs

[@waowwaow8239]

Hop Barış hocamdan geldik buradayız

[@sumeyye_nur_esin]

BARIŞ HOCAM SAĞOLSUN BİZİ DENEYLERE ORTAK ETTİ

[@kerimbeyazt4175]

barış hoca da olmasa geleneksel eğitim metotlarına maruz kalmaya devam edeceğiz

[@bencekomigim]

ben de ordan geldim sdkfsdmögs

[@mer.2024yks]

Ben de chhdj

[@sevvaltann]

barış hocam yolladı bizi buralara

[@noth3285]

o kim

[@sevvaltann]

@@noth3285 barış hoca biyoloji

[@tyt-ayt6142]

Evetttt

[@doktorolucam]

Dr biyoloji

[@mer.2024yks]

Ayn

[@nisan-m]

barış hocam sağ olsun

[@polkadots7792]

EVET EVET!

[@tanjat123]

Barış Hocadan gelen arkadaşım sana da selam olsun

[@hedeftp6934]

Barış hocamdan buraya transfer olduk

[@ercan..]

Barış hocadan geldik.

[@berin2004]

barış hocam sağ olsun deneyleri görebiliyoz

[@mezunbiri]

Barış hocadan gelenler yazsın buraya yks savaşçısiyizzz

[@mstftrk2843]

Barış Hocam'dan geldim.❤

[@yavuzsolak]

We brought the greetings of our valuable teacher Barış M. Kapan

[@blairwaldorrf]

barış hocadan geldim

[@afiyetolsun00]

Barıs hoca kalitesiyle buradayız

[@esmaartunay9558]

barış hocadan gelenler

[@Erchnnsogl]

@DR.BİYOLOJİ HOCAMDAN SELAMLAR

[@rascalriley]

Barış Hoca'dan gelenler

[@Ksjdjdjjf]

Barış hocadan gelenlere sa

[@gulsumbalc3786]

Barış hocam da barış hocam

[@MehmetTugrull]

aşwldşlawdşşad

[@fadentunc578]

Dr. Biyoloji 💐

[@firemeetgasoline8502]

Barış hocadan gelenler ses verin 🤝

[@againstepbystep]

ben ben ben

[@Shyeers]

ben dee 🤘

[@YKS2006]

Kazandınız mıı

[@bencekomigim]

asla cevap vermezler... @@YKS2006

[@YKS2006]

Dr. Biyoloji Dı dım tı tıs 🥁

[@hamitmertoguz5980]

DR Biyoloji den gelenler

[@AslgulSert-ii8tx]

Barış hocam bir tane 😊😊

[@elenielenaki6673]

I just realized why I was doing this in lab because the instructor did not explain a thing! Thanks soo much! God bless 🙏🏻

[@xxTAARGUS]

Thankfully my instructor is a boss and covered all of this and used this to show the process, hope you get a better teacher next semester.

[@onyebuchiblog]

I'm interestingly interested in this

[@DrSohaAli786]

How many are there after studing biotechnology 12 class

[@DrSohaAli786]

@DoktorOnia nice

[@k47mc60]

MSC microbio

[@Toluene26]

Me too

[@CEO_CRESCENT]

Me

[@sumitroy5204]

Nah , I'm here for college lab exam

[@hakanyigit8901]

Barış hocamda barış hocammmmm

[@esra.793]

Barış Hocamda Barış Hocam

[@yksascisi]

Barış hocam 🤟🤟

[@caganyavuzer]

Barış hocam gönderdi O7

[@LeylaKonyal]

I'm coming from Barış hoca youtube chanel❤

[@busra9257]

Came from "DR. BİYOLOJİ" chanel 🤙

[@roseofheaven_]

Dr Biyoloji🫡

[@eylulvehayatindakiguzellikler]

barış hocamdan geldikkk

[@Arpnasingh20]

Amazing...to see the DNA fragments...1. Smaller the fragment size ,the farther it moves. 2.Agarose is natural polymer extracted from Sea weeds. 3.Separated DNA fragments can be visualised after staining with ethidium bromide.

[@aleena8065]

NCERT! 😂👌

[@Mego1031]

Who the hell still uses ethidium bromide!??!? SMH...SYBR Safe has been around for like a couple of decades now...there's absolutely no need to keep using the HIGHLY carcinogenic EtBr...wow...

[@walidnadirulahnaf3978]

1. siapkan sub sel mini 2. sejajarkan gel sehingga sumur paling dekat dengan elektrode negatif 3. tempatkan egl agarosa ke ruang gel 4. tambahkan buffer elektroforesis ke reservoir sampai reservoir tertutup buffer elektroforesis sedalam 2mm 5. tempatkan sampel sesuai urutan yang benar 6. tempatkan sampel ke dalam sumur dengan menggunakan mikropipet. jagalah pipet tetep tegak lurus terhadap lubang 7. pasang tutup ruang gel sesuai dengan elektrodanya (hitam ke hitam, merah ke merah) 8. sambungkan elektrode ke catu daya 9. nyalakan catu daya 10. atur tegangan konstan sebesar 100V 11. atur timer menjadi 60s 12. amati perubahan yang terjadi

[@poetrylover5561]

Excellent description on GEL ELECTROPHORESIS...MANY THANKS FOR UPLOADING THIS LECTURE ❤️

[@nredirul5069]

Bütün barış hoca yorumlarını beğendim👍

[@shayisbored]

How nostalgic. I did this as a practical exam when I was in college and completely ruined the agarose gel 😂😭😭. My prof got really mad.

[@walterwhite4699]

Damn can't wait till I go to college...

[@timecode37]

What happened exactly? How does one ruin the agarose?

[@sondos8928]

Watching it during quarantine 😭😭😝

[@buba_Dukz]

haha

[@sondos8928]

Omg i passed that semester and still corona didn’t ended

[@nutallergy420]

@@sondos8928 hahahaha I’m doing it for mine right now

[@walterwhite4699]

@@sondos8928 "ended"

[@irishtaco9056]

im sorry quarantine was a year ago-? holy shit-

[@Sexettin]

Barış Hoca sayesinde geldik

[@bluegenes2273]

1:56 oh, that is so damn satisfying.

[@sam_khan_1995]

When he pippet sucked the entire sample I said fukkkk so perfect

[@papoutsothiki]

Please DO NOT stick your pipette tip in the well while loading,you might puncture it, not to mention the sample might hit the well and spurt outside the well.pour enough buffer so that your tip is immersed but is still right above the well and release sample slowly. The loading buffer in your sample, in this case the blue stuff, contains glycerol which is heavy and 'leads' your DNA safely in the well.

[@whatevervlogs9663]

I do what I want

[@papoutsothiki]

@@whatevervlogs9663 you can choose to learn or not. your call mate 🤷

[@srividhyanarayanan7337]

Actually I'm curious to know how to load the sample.if I simply put the sample in the well doesn't it get mixed with buffer.or u directly put the sample inside the agarose??

[@papoutsothiki]

@@srividhyanarayanan7337 your sample should already have buffer in it because you use the Loading Buffer LB I use a 1 microliter of 6x loading buffer (trisborate a couple of dyes and glycerol) and 5 microliters sample therefore your LB is diluted to 1x. If you are using 1x TBE or TAE the liquids in theory are close isotonically even though the actual samples vary. do not worry. the glycerol in your LB is heavy enough it will pull the sample into your well because gravity. Just practise a bit - put the tip above the well IN THE BUFFER and slowly release sample (no bubbles) you will see your sample-dye sink to the bottom of well. Maniatis protocols explain this nicely. Let me know if you need a recipe for LB .

[@k47mc60]

@@papoutsothiki I don't think that's a thing, the pipette is supposed to puncture through the gel to allow the plasmid dna/ genomic dna to enter the electrophoresis casette

[@punamchand9461]

Nomesh sir PW show this in class 😎😎 rewatching this video after class ❤❤

[@HindustanKarakshak]

Nomesh sir jindabad

[@pisser98]

instead of pushing the pipette all the way through when loading the wells, you could release pressure to suck the bubble back in. that way you avoid marked sample spilling out of the wells - not that it makes a huge difference, i just like to keep the buffer clean.

[@Ronnicus]

Or you could not push to the second stop

[@HayDayEveryday]

@@Ronnicus lol

[@onyebuchiblog]

Lovely, I'm interestingly interested in this. I just finished running the three practicals now, on center for molecular bioscience and biotechnology lab in SCH

[@potatoboi9298]

Hi can i ask you some questions maybe in the future for academic purposes only. thank you. is it okay if i can contact you through email?

[@mehmethanyildirim1353]

Thank you so much. I learned lessons but i never applied and i am still scared 😅

[@sudiptachanda3486]

What should we do if there more bubble in red electrode? Please make a video on sample preparation also

[@drd6482]

SILENCE #yousaidit #haahhahah #wavy #furthersilence #yeahboi

[@kiwibranches6013]

#yeahboi

[@jasminanil5411]

very informative . thanks a lot.

[@HayDayEveryday]

Thanks

[@sevvalesi28]

barış hocadan geldikk

[@iclalcim]

Harika bi sey

[@katarina6587]

thanks my dude

[@amritas2400]

Thank you! This helped a lot! But hey, where's ethidium bromide and UV rays? And bright orange bands of DNA?

[@Gayu_raje]

Yes.. Atlast we visualize clear bands by uv rays .. They didn't mentioned it

[@Icecube88]

i messed this up in lab. wasted 3 weeks preparing my cheek cells to see if i was a taster or not. smh.

[@yessycueva2600]

I love it =) It's so interesting and beautiful at the same time =)

[@MrHeatAz]

So are you :p

[@rishibhargav5714]

Watching this after reading Bio-Tech chapter...

[@aycelllaaa]

Barış hocam yollarsahoop buradayız

[@abigailtsado8720]

Very understanding , thank you

[@Ripjaws3199]

When we did it we couldn't really find the wells and struggled for almost an hour and a half to find the wells.

[@arresteddevelopment2158]

So exciting, this!

[@i.kamalesh1930]

For the bands to be visible…. No need for ethidium bromide and uv light exposure?

[@arresteddevelopment2158]

Thank you for this

[@charliesun1304]

Very cool video, well done!

[@faizaquddus2848]

A Clear video..

[@anjalichaudhary5985]

osmmm video! plzz upload more more dis kind of video so that it is to understand

[@boyinalabcoatboyinalabcoat393]

for the blanck I use the leader + load dye. for samples of dna you need load dye + dna + enzime + enzime buffer, I am missing something?

[@hemo2458]

fantastic

[@rheacrisolo1882]

I'm watching this for an activity, but I badly want to experience doing it.

[@girijasankarpanigrahi4393]

Yep. Our school brought us to the practical room to make us watch this but anything was barely visible :') 👍

[@HemantSharma-ro1dk]

This is very nailed and good knowledge thanks 🙂

[@legostar8142]

Which dye do you use? And do you add the dye into the centrifuge?

[@girijasankarpanigrahi4393]

Ethirium Bromide as the movement indicator and Bromopropanal as the blue dye.

[@neelam722]

Is fast blast DNA stain as good as ethidium bromide,i mean can it practically replace ethidium bromide,are there any hazzards concerned with this stain as it is with ethidium bromide

[@Reivivus]

+Neelam Singh, In our University Biochemistry lab today, we used SYBR safe DNA Gel Stain. It is less mutagenic than ethidium bromide, and can be used under blue light just like ethidium bromide. I would feel much more comfortable buying this solution from Thermofisher Scientific. You can buy the solution here, as well as see the tests used to validate its efficiency on this page, as well. (Notice how the page is padlocked when you follow the link.) www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html

[@dertlibirogrenci9764]

Allah Allah

[@bong__singha2390]

After nomesh sir lec

[@NIPUNICHATHUNIKA]

Thanks. It’s very clear

[@meghanaakshaya1915]

Very good information tq it is useful of my lesson .

[@Jemiller2012]

very well done video

[@hemantanayak3997]

excellent videos

[@d.playerallrounder2407]

🙏thank you 🙏

[@mattmeza6825]

This was awesome

[@Biologyandbiotechnology-qc5jo]

good expression

[@claudeflex]

BARIŞ HOCAM ❤✋

[@mehulmakvana8583]

That's my favourite experiment

[@MrRisha100]

Thank you.. Well explained

[@deisigomez7345]

Why does DNA travel down the gel towards the positive charge

[@tishashah9842]

because DNA itself is negatively charged

[@ajsidhu4171]

i never pressed run on the generator and I was so confused when my samples never ran :(

[@drd6482]

wavy #science #ducks #ligit @yes

[@drd6482]

#yeahboi

[@pharmasolutionpakistan7668]

Good effort

[@saradarvishvand5653]

very very good

[@hiralsheth3620]

Y do we get purple n blue bands.. In AGE, what does these 2 bands indicate, WHERE is the actual DNA

[@ayat4522]

Ethidium bromide is used to stain dna to visualize it .isn't it actual dna?

[@shanoosingh9076]

What a wonderful day???????????????!!!

[@HussainAli-xf6bk]

very Informative

[@KarthiKeyan-kq9yu]

really good

[@medicinestudent8349]

Thank you!

[@xmem1r]

Any NCERT Biology student here ?

[@anamikapushkar222]

thanks .. its good to learn the exact technique.

[@Aevan248]

that was great thank you

[@qa14tusharnaiknaware25]

Very Interesting 👍🙏

[@harkamanghag6877]

Very informative.