Agarose Gel Electrophoresis

[@waowwaow8239]

Hop Barış hocamdan geldik buradayız

[@kerimbeyazt4175]

barış hoca da olmasa geleneksel eğitim metotlarına maruz kalmaya devam edeceğiz

[@1eternalbs]

ben de ordan geldim sdkfsdmögs

[@mer.2024yks]

Ben de chhdj

[@Myefty_bs]

Bende

[@sumeyye_nur_esin]

BARIŞ HOCAM SAĞOLSUN BİZİ DENEYLERE ORTAK ETTİ

[@sevvaltann]

barış hocam yolladı bizi buralara

[@noth3285]

o kim

[@sevvaltann]

@@noth3285 barış hoca biyoloji

[@tyt-ayt6142]

Evetttt

[@doktorolucam]

Dr biyoloji

[@mer.2024yks]

Ayn

[@nisan-m]

barış hocam sağ olsun

[@polkadots7792]

EVET EVET!

[@yavuzsolak]

We brought the greetings of our valuable teacher Barış M. Kapan

[@elzemfezademir]

diğer ismi ne hocanın

[@yavuzsolak]

@@elzemfezademir Mahmut 🍀

[@birslskslsksk]

barış hocam sağ olsun deneyleri görebiliyoz

[@ercan..]

Barış hocadan geldik.

[@MSASTUDIO-47]

BARIŞ HOCA OLMASA KANAL 0.5 MİLYON GÖRÜNTÜLENMEDE KALACAKTI Bİ TEŞEKKÜR EDİN BENCE

[@mstftrk2843]

Barış Hocam'dan geldim.❤

[@mezunbiri]

Barış hocadan gelenler yazsın buraya yks savaşçısiyizzz

[@tanjat123]

Barış Hocadan gelen arkadaşım sana da selam olsun

[@afiyetolsun00]

Barıs hoca kalitesiyle buradayız

[@esmaartunay9558]

barış hocadan gelenler

[@hedeftp6934]

Barış hocamdan buraya transfer olduk

[@rascalriley]

Barış Hoca'dan gelenler

[@elenielenaki6673]

I just realized why I was doing this in lab because the instructor did not explain a thing! Thanks soo much! God bless 🙏🏻

[@xxTAARGUS]

Thankfully my instructor is a boss and covered all of this and used this to show the process, hope you get a better teacher next semester.

[@onyebuchiblog]

I'm interestingly interested in this

[@busra9257]

Came from "DR. BİYOLOJİ" chanel 🤙

[@firemeetgasoline8502]

Barış hocadan gelenler ses verin 🤝

[@againstepbystep]

ben ben ben

[@Shyeers]

ben dee 🤘

[@YKS2006]

Kazandınız mıı

[@1eternalbs]

asla cevap vermezler... @@YKS2006

[@MSASTUDIO-47]

burdayız

[@SohaAli786]

How many are there after studing biotechnology 12 class

[@SohaAli786]

@DoktorOnia nice

[@k47mc60]

MSC microbio

[@Toluene26]

Me too

[@CEO_CRESCENT]

Me

[@sumitroy5204]

Nah , I'm here for college lab exam

[@gulsumbalc3786]

Barış hocam da barış hocam

[@MehmetTugrull]

aşwldşlawdşşad

[@walidnadirulahnaf3978]

1. siapkan sub sel mini 2. sejajarkan gel sehingga sumur paling dekat dengan elektrode negatif 3. tempatkan egl agarosa ke ruang gel 4. tambahkan buffer elektroforesis ke reservoir sampai reservoir tertutup buffer elektroforesis sedalam 2mm 5. tempatkan sampel sesuai urutan yang benar 6. tempatkan sampel ke dalam sumur dengan menggunakan mikropipet. jagalah pipet tetep tegak lurus terhadap lubang 7. pasang tutup ruang gel sesuai dengan elektrodanya (hitam ke hitam, merah ke merah) 8. sambungkan elektrode ke catu daya 9. nyalakan catu daya 10. atur tegangan konstan sebesar 100V 11. atur timer menjadi 60s 12. amati perubahan yang terjadi

[@aysenilhakbilir7839]

barış hocamın selamını getirdim ey ahali

[@Ksjdjdjjf]

Barış hocadan gelenlere sa

[@Erchnnsogl]

@DR.BİYOLOJİ HOCAMDAN SELAMLAR

[@hamitmertoguz5980]

DR Biyoloji den gelenler

[@MSASTUDIO-47]

burdayız

[@fadentunc578]

Dr. Biyoloji 💐

[@Arpnasingh20]

Amazing...to see the DNA fragments...1. Smaller the fragment size ,the farther it moves. 2.Agarose is natural polymer extracted from Sea weeds. 3.Separated DNA fragments can be visualised after staining with ethidium bromide.

[@aleena8065]

NCERT! 😂👌

[@Mego1031]

Who the hell still uses ethidium bromide!??!? SMH...SYBR Safe has been around for like a couple of decades now...there's absolutely no need to keep using the HIGHLY carcinogenic EtBr...wow...

[@AslgulSert-ii8tx]

Barış hocam bir tane 😊😊

[@poetrylover5561]

Excellent description on GEL ELECTROPHORESIS...MANY THANKS FOR UPLOADING THIS LECTURE ❤️

[@abcde-cq6ns]

Yorumların çoğu "Barış hocadan geldim" 😂

[@sondos8928]

Watching it during quarantine 😭😭😝

[@buba_Dukz]

haha

[@sondos8928]

Omg i passed that semester and still corona didn’t ended

[@nutallergy420]

@@sondos8928 hahahaha I’m doing it for mine right now

[@walterwhite4699]

@@sondos8928 "ended"

[@irishtaco9056]

im sorry quarantine was a year ago-? holy shit-

[@Sweet_tooth_04]

Barış hocaya teşekkürler ❤

[@Baranmencur]

barış hocadan geldik...

[@papoutsothiki]

Please DO NOT stick your pipette tip in the well while loading,you might puncture it, not to mention the sample might hit the well and spurt outside the well.pour enough buffer so that your tip is immersed but is still right above the well and release sample slowly. The loading buffer in your sample, in this case the blue stuff, contains glycerol which is heavy and 'leads' your DNA safely in the well.

[@whatevervlogs9663]

I do what I want

[@papoutsothiki]

@@whatevervlogs9663 you can choose to learn or not. your call mate 🤷

[@srividhyanarayanan7337]

Actually I'm curious to know how to load the sample.if I simply put the sample in the well doesn't it get mixed with buffer.or u directly put the sample inside the agarose??

[@papoutsothiki]

@@srividhyanarayanan7337 your sample should already have buffer in it because you use the Loading Buffer LB I use a 1 microliter of 6x loading buffer (trisborate a couple of dyes and glycerol) and 5 microliters sample therefore your LB is diluted to 1x. If you are using 1x TBE or TAE the liquids in theory are close isotonically even though the actual samples vary. do not worry. the glycerol in your LB is heavy enough it will pull the sample into your well because gravity. Just practise a bit - put the tip above the well IN THE BUFFER and slowly release sample (no bubbles) you will see your sample-dye sink to the bottom of well. Maniatis protocols explain this nicely. Let me know if you need a recipe for LB .

[@k47mc60]

@@papoutsothiki I don't think that's a thing, the pipette is supposed to puncture through the gel to allow the plasmid dna/ genomic dna to enter the electrophoresis casette

[@bluegenes2273]

1:56 oh, that is so damn satisfying.

[@sam_khan_1995]

When he pippet sucked the entire sample I said fukkkk so perfect

[@yasar_yasamaz]

Barıs hocamdan geldim.

[@LeylaKonyal]

I'm coming from Barış hoca youtube chanel❤

[@YKS2006]

Dr. Biyoloji Dı dım tı tıs 🥁

[@cemre3593]

Tamamdır hocam bunuda izledik✔️✔️

[@BennurErbil-d1u]

Barış hocadan geldik✨️

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Bütün barış hoca yorumlarını beğendim👍

[@elvinn212.]

Barış hocam gönderirde gelmezmiyiz ❤

[@user16789-x]

BARIŞ HOCA MENTİONED

[@azra-pk2cz]

As bayrakları

[@shayisbored]

How nostalgic. I did this as a practical exam when I was in college and completely ruined the agarose gel 😂😭😭. My prof got really mad.

[@walterwhite4699]

Damn can't wait till I go to college...

[@timecode37]

What happened exactly? How does one ruin the agarose?

[@pisser98]

instead of pushing the pipette all the way through when loading the wells, you could release pressure to suck the bubble back in. that way you avoid marked sample spilling out of the wells - not that it makes a huge difference, i just like to keep the buffer clean.

[@Ronnicus]

Or you could not push to the second stop

[@HayDayEveryday]

@@Ronnicus lol

[@punamchand9461]

Nomesh sir PW show this in class 😎😎 rewatching this video after class ❤❤

[@sudiptachanda3486]

What should we do if there more bubble in red electrode? Please make a video on sample preparation also

[@onyebuchiblog]

Lovely, I'm interestingly interested in this. I just finished running the three practicals now, on center for molecular bioscience and biotechnology lab in SCH

[@potatoboi9298]

Hi can i ask you some questions maybe in the future for academic purposes only. thank you. is it okay if i can contact you through email?

[@mehmethanyildirim1353]

Thank you so much. I learned lessons but i never applied and i am still scared 😅

[@Myefty_bs]

Eyvallah Barış Hocammm ❤❤

[@esra.793]

Barış Hocamda Barış Hocam

[@eylulvehayatindakiguzellikler]

barış hocamdan geldikkk

[@xquartest]

adamsınız barış hocam

[@yufusye]

Saygılar barıs hocam

[@amritas2400]

Thank you! This helped a lot! But hey, where's ethidium bromide and UV rays? And bright orange bands of DNA?

[@Gayu_raje]

Yes.. Atlast we visualize clear bands by uv rays .. They didn't mentioned it

[@Hhajsjrkutl]

Barış hocamdan geldimmm

[@barışhocanınmühendisi]

Barış Hocadan geldik efenim

[@Icecube88]

i messed this up in lab. wasted 3 weeks preparing my cheek cells to see if i was a taster or not. smh.

[@caganyavuzer]

Barış hocam gönderdi O7

[@hussainabbas454]

What is the dimention of uv tray in the video.

[@i.kamalesh1930]

For the bands to be visible…. No need for ethidium bromide and uv light exposure?

[@boyinalabcoatboyinalabcoat393]

for the blanck I use the leader + load dye. for samples of dna you need load dye + dna + enzime + enzime buffer, I am missing something?

[@neelam722]

Is fast blast DNA stain as good as ethidium bromide,i mean can it practically replace ethidium bromide,are there any hazzards concerned with this stain as it is with ethidium bromide

[@Reivivus]

+Neelam Singh, In our University Biochemistry lab today, we used SYBR safe DNA Gel Stain. It is less mutagenic than ethidium bromide, and can be used under blue light just like ethidium bromide. I would feel much more comfortable buying this solution from Thermofisher Scientific. You can buy the solution here, as well as see the tests used to validate its efficiency on this page, as well. (Notice how the page is padlocked when you follow the link.) www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html

[@jasminanil5411]

very informative . thanks a lot.

[@HayDayEveryday]

Thanks

[@yksascisi]

Barış hocam 🤟🤟

[@legostar8142]

Which dye do you use? And do you add the dye into the centrifuge?

[@girijasankarpanigrahi4393]

Ethirium Bromide as the movement indicator and Bromopropanal as the blue dye.

[@dimpalpanchal1795]

Hello professor sir I am am student and our mam suggested to watch this video and it's makes me more curious and I want to learn more and more about this can you help me sir ?

[@Ripjaws3199]

When we did it we couldn't really find the wells and struggled for almost an hour and a half to find the wells.

[@maryamsalih3801]

how I can label the product of gel electrophoresis by which program if my gel image was taken by JPA please kindly for you recommend me thanks

[@سندس-ك9ج]

انا هنا لاني ادرس هذا الدرس في الاحياء شكرا لك ساعدني هذا لافهم

[@Heisenbergmiii]

BARIŞ HOCAM ❤✋

[@hakanyigit8901]

Barış hocamda barış hocammmmm

[@adanqureshi2425]

What do we need the buffer for ?

[@salazardelmar7235]

Why do we see more bubbles at the black electrode ?

[@katarina6587]

thanks my dude

[@dlhawari5086]

Hello, any distributor of this instrumentation and consumable in Indonesia? Thank you

[@Getgot-en1kv]

I don’t need sybr dye for this step right?

[@gokcelektrogitarcalmakistiyor]

Barış hocam

[@paouw6877]

what can be used as buffer solution except for tbe and tae?

[@Daretofeelalive]

Water

[@aycelllaaa]

Barış hocam yollarsahoop buradayız

[@ulskc]

GENÇLER MERHABA

[@yessycueva2600]

I love it =) It's so interesting and beautiful at the same time =)

[@MrHeatAz]

So are you :p

[@deisigomez7345]

Why does DNA travel down the gel towards the positive charge

[@tishashah9842]

because DNA itself is negatively charged

[@hiralsheth3620]

Y do we get purple n blue bands.. In AGE, what does these 2 bands indicate, WHERE is the actual DNA

[@ayat4522]

Ethidium bromide is used to stain dna to visualize it .isn't it actual dna?

[@Diyars04]

Barış hocamm saolsun

[@sevvalesi28]

barış hocadan geldikk

[@yksliguts]

kimyadaki anot katot devresinin nerde kullanıldığını öğrenmiş oldum ek olarak

[@yagmurtosun4221]

Barış hocanın askerleriyiz

[@riyasharma-rr3ji]

why we cannt load dna samples in the wells before loading the buffer?

[@r.sivamurugansivamurugan2551]

it is because the buffer maintains the pH

[@krishnamani8150]

Please how to prepare agarose gel and buffer to post the vedio

[@iclalcim]

Harika bi sey

[@uykusuzbirdoktor7879]

herkes barış hocadan gelmiş sdkfnekjgh

[@bukimya]

Dr Biyoloji🫡

[@AhmetDeniz-j5u]

Barış Hocadan geldik...

[@Gayu_raje]

How can u add sample before casting the gel

[@Gayu_raje]

@@BioRadLaboratoriesCorp if the sample is added after the buffer.. Sample wouldn't mix with it?

[@mondarahmed7245]

What happens if I place samples in the wells first -- that is ahead of the buffer?

[@the-sprocketeer]

Sorry to reply a year later, but in case you or others are wondering, I do NOT recommend this. If the samples are already in the wells when you add the buffer, the movement of the fluid could cause the samples to spill out of the wells, leading to contamination

[@HemantSharma-ro1dk]

This is very nailed and good knowledge thanks 🙂

[@HindustanKarakshak]

Nomesh sir jindabad

[@arresteddevelopment2158]

So exciting, this!