Agarose Gel Electrophoresis

For more information, visit www.bio-rad.com....
This video demonstrates how to load and run DNA samples on an agarose gel. Basic information about the charge of DNA and how it will run in an horizontal electrophoresis cell is explained.
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[@waowwaow8239]

Hop Barış hocamdan geldik buradayız

[@bs_lordvoldemort]

BARIŞ HOCA OLMASA KANAL 0.5 MİLYON GÖRÜNTÜLENMEDE KALACAKTI Bİ TEŞEKKÜR EDİN BENCE

[@sumeyye_nur_esin]

BARIŞ HOCAM SAĞOLSUN BİZİ DENEYLERE ORTAK ETTİ

[@kerimbeyazt4175]

barış hoca da olmasa geleneksel eğitim metotlarına maruz kalmaya devam edeceğiz

[@1eternalbs]

ben de ordan geldim sdkfsdmögs

[@mer.2024yks]

Ben de chhdj

[@Myefty_bs]

Bende

[@sevvaltann]

barış hocam yolladı bizi buralara

[@noth3285]

o kim

[@sevvaltann]

@@noth3285 barış hoca biyoloji

[@tyt-ayt6142]

Evetttt

[@doktorolucam]

Dr biyoloji

[@mer.2024yks]

Ayn

[@yavuzsolak]

We brought the greetings of our valuable teacher Barış M. Kapan

[@elzemfezademir]

diğer ismi ne hocanın

[@yavuzsolak]

@@elzemfezademir Mahmut 🍀

[@sudeninkloroplastı]

Barış hocaya teşekkür edin bu izlenme için.

[@nisan-m]

barış hocam sağ olsun

[@polkadots7792]

EVET EVET!

[@ercan..]

Barış hocadan geldik.

[@afiyetolsun00]

Barıs hoca kalitesiyle buradayız

[@mstftrk2843]

Barış Hocam'dan geldim.❤

[@birslskslsksk]

barış hocam sağ olsun deneyleri görebiliyoz

[@busra9257]

Came from "DR. BİYOLOJİ" chanel 🤙

[@tanjat123]

Barış Hocadan gelen arkadaşım sana da selam olsun

[@mezunbiri]

Barış hocadan gelenler yazsın buraya yks savaşçısiyizzz

[@elenielenaki6673]

I just realized why I was doing this in lab because the instructor did not explain a thing! Thanks soo much! God bless 🙏🏻

[@xxTAARGUS]

Thankfully my instructor is a boss and covered all of this and used this to show the process, hope you get a better teacher next semester.

[@onyebuchiblog]

I'm interestingly interested in this

[@esmaartunay9558]

barış hocadan gelenler

[@hedeftp6934]

Barış hocamdan buraya transfer olduk

[@Alexaonthegame]

Came from Dr.Biyoloji❤

[@aysenilhakbilir7839]

barış hocamın selamını getirdim ey ahali

[@walidnadirulahnaf3978]

1. siapkan sub sel mini 2. sejajarkan gel sehingga sumur paling dekat dengan elektrode negatif 3. tempatkan egl agarosa ke ruang gel 4. tambahkan buffer elektroforesis ke reservoir sampai reservoir tertutup buffer elektroforesis sedalam 2mm 5. tempatkan sampel sesuai urutan yang benar 6. tempatkan sampel ke dalam sumur dengan menggunakan mikropipet. jagalah pipet tetep tegak lurus terhadap lubang 7. pasang tutup ruang gel sesuai dengan elektrodanya (hitam ke hitam, merah ke merah) 8. sambungkan elektrode ke catu daya 9. nyalakan catu daya 10. atur tegangan konstan sebesar 100V 11. atur timer menjadi 60s 12. amati perubahan yang terjadi

[@Yagmur_b.c]

Barış hocam sayesinde daha iyi anladım şimdi

[@rascalriley]

Barış Hoca'dan gelenler

[@firemeetgasoline8502]

Barış hocadan gelenler ses verin 🤝

[@againstepbystep]

ben ben ben

[@Shyeers]

ben dee 🤘

[@YKS2006]

Kazandınız mıı

[@1eternalbs]

asla cevap vermezler... @@YKS2006

[@bs_lordvoldemort]

burdayız

[@gulsumbalc3786]

Barış hocam da barış hocam

[@MehmetTugrull]

aşwldşlawdşşad

[@yufusye]

Saygılar barıs hocam

[@Erchnnsogl]

@DR.BİYOLOJİ HOCAMDAN SELAMLAR

[@Birazdaaytmatli]

Before "hadi soru cozelim!"

[@yagmurbb4818]

baris hocadan selamlar

[@Ksjdjdjjf]

Barış hocadan gelenlere sa

[@Cerrahpaşatıp-m5p]

Barış hocamdan geldimmm

[@SohaAli786]

How many are there after studing biotechnology 12 class

[@SohaAli786]

@DoktorOnia nice

[@k47mc60]

MSC microbio

[@Toluene26]

Me too

[@CEO_CRESCENT]

Me

[@sumitroy5204]

Nah , I'm here for college lab exam

[@AhmetDeniz-j5u]

Barış Hocadan geldik...

[@Hyxmzöskfkckx]

evet bende Barış Hocamdan geldim

[@abcde-cq6ns]

Yorumların çoğu "Barış hocadan geldim" 😂

[@fadentunc578]

Dr. Biyoloji 💐

[@cemre3593]

Tamamdır hocam bunuda izledik✔️✔️

[@Arpnasingh20]

Amazing...to see the DNA fragments...1. Smaller the fragment size ,the farther it moves. 2.Agarose is natural polymer extracted from Sea weeds. 3.Separated DNA fragments can be visualised after staining with ethidium bromide.

[@aleena8065]

NCERT! 😂👌

[@Mego1031]

Who the hell still uses ethidium bromide!??!? SMH...SYBR Safe has been around for like a couple of decades now...there's absolutely no need to keep using the HIGHLY carcinogenic EtBr...wow...

[@yagmurtosun4221]

Barış hocanın askerleriyiz

[@hamitmertoguz5980]

DR Biyoloji den gelenler

[@bs_lordvoldemort]

burdayız

[@Baranmencur]

barış hocadan geldik...

[@YGFARUK]

Buradan Barış hocama selamlar

[@xquartest]

adamsınız barış hocam

[@yasar_yasamaz]

Barıs hocamdan geldim.

[@ulskc]

GENÇLER MERHABA

[@poetrylover5561]

Excellent description on GEL ELECTROPHORESIS...MANY THANKS FOR UPLOADING THIS LECTURE ❤️

[@YKS2006]

Dr. Biyoloji Dı dım tı tıs 🥁

[@AslgulSert-ii8tx]

Barış hocam bir tane 😊😊

[@Sweet_tooth_04]

Barış hocaya teşekkürler ❤

[@shayisbored]

How nostalgic. I did this as a practical exam when I was in college and completely ruined the agarose gel 😂😭😭. My prof got really mad.

[@walterwhite4699]

Damn can't wait till I go to college...

[@timecode37]

What happened exactly? How does one ruin the agarose?

[@sondos8928]

Watching it during quarantine 😭😭😝

[@buba_Dukz]

haha

[@sondos8928]

Omg i passed that semester and still corona didn’t ended

[@nutallergy420]

@@sondos8928 hahahaha I’m doing it for mine right now

[@walterwhite4699]

@@sondos8928 "ended"

[@irishtaco9056]

im sorry quarantine was a year ago-? holy shit-

[@LeylaKonyal]

I'm coming from Barış hoca youtube chanel❤

[@punamchand9461]

Nomesh sir PW show this in class 😎😎 rewatching this video after class ❤❤

[@Diyars04]

Barış hocamm saolsun

[@nredirul5069]

Bütün barış hoca yorumlarını beğendim👍

[@bluegenes2273]

1:56 oh, that is so damn satisfying.

[@sam_khan_1995]

When he pippet sucked the entire sample I said fukkkk so perfect

[@pisser98]

instead of pushing the pipette all the way through when loading the wells, you could release pressure to suck the bubble back in. that way you avoid marked sample spilling out of the wells - not that it makes a huge difference, i just like to keep the buffer clean.

[@Ronnicus]

Or you could not push to the second stop

[@HayDayEveryday]

@@Ronnicus lol

[@BennurErbil-d1u]

Barış hocadan geldik✨️

[@vladimirlenin4241]

Barışın askerleri geldi.

[@onyebuchiblog]

Lovely, I'm interestingly interested in this. I just finished running the three practicals now, on center for molecular bioscience and biotechnology lab in SCH

[@potatoboi9298]

Hi can i ask you some questions maybe in the future for academic purposes only. thank you. is it okay if i can contact you through email?

[@yks2024z]

Barış hocama saygilarrr🫶🏻🫡

[@Omer.00007]

Dr biyobarış

[@sudiptachanda3486]

What should we do if there more bubble in red electrode? Please make a video on sample preparation also

[@eylulvehayatindakiguzellikler]

barış hocamdan geldikkk

[@yksascisi]

Barış hocam 🤟🤟

[@zambak-k5i]

dr biyoloji BABAAAG

[@سندس-ك9ج]

انا هنا لاني ادرس هذا الدرس في الاحياء شكرا لك ساعدني هذا لافهم

[@Myefty_bs]

Eyvallah Barış Hocammm ❤❤

[@Heisenbergmiii]

BARIŞ HOCAM ❤✋

[@elvinn212.]

Barış hocam gönderirde gelmezmiyiz ❤

[@papoutsothiki]

Please DO NOT stick your pipette tip in the well while loading,you might puncture it, not to mention the sample might hit the well and spurt outside the well.pour enough buffer so that your tip is immersed but is still right above the well and release sample slowly. The loading buffer in your sample, in this case the blue stuff, contains glycerol which is heavy and 'leads' your DNA safely in the well.

[@whatevervlogs9663]

I do what I want

[@papoutsothiki]

@@whatevervlogs9663 you can choose to learn or not. your call mate 🤷

[@srividhyanarayanan7337]

Actually I'm curious to know how to load the sample.if I simply put the sample in the well doesn't it get mixed with buffer.or u directly put the sample inside the agarose??

[@papoutsothiki]

@@srividhyanarayanan7337 your sample should already have buffer in it because you use the Loading Buffer LB I use a 1 microliter of 6x loading buffer (trisborate a couple of dyes and glycerol) and 5 microliters sample therefore your LB is diluted to 1x. If you are using 1x TBE or TAE the liquids in theory are close isotonically even though the actual samples vary. do not worry. the glycerol in your LB is heavy enough it will pull the sample into your well because gravity. Just practise a bit - put the tip above the well IN THE BUFFER and slowly release sample (no bubbles) you will see your sample-dye sink to the bottom of well. Maniatis protocols explain this nicely. Let me know if you need a recipe for LB .

[@k47mc60]

@@papoutsothiki I don't think that's a thing, the pipette is supposed to puncture through the gel to allow the plasmid dna/ genomic dna to enter the electrophoresis casette

[@Icecube88]

i messed this up in lab. wasted 3 weeks preparing my cheek cells to see if i was a taster or not. smh.

[@mehmethanyildirim1353]

Thank you so much. I learned lessons but i never applied and i am still scared 😅

[@dimpalpanchal1795]

Hello professor sir I am am student and our mam suggested to watch this video and it's makes me more curious and I want to learn more and more about this can you help me sir ?

[@caganyavuzer]

Barış hocam gönderdi O7

[@esra.793]

Barış Hocamda Barış Hocam

[@silveramayksçalışan]

Barış Hocadan geldik efenim

[@i.kamalesh1930]

For the bands to be visible…. No need for ethidium bromide and uv light exposure?

[@Ripjaws3199]

When we did it we couldn't really find the wells and struggled for almost an hour and a half to find the wells.

[@BluishKedy]

Tüm diller var sadece Türkçe çevirisi yok üzücü...

[@gokcelektrogitarcalmakistiyor]

Barış hocam

[@hussainabbas454]

What is the dimention of uv tray in the video.

[@hakanyigit8901]

Barış hocamda barış hocammmmm

[@amritas2400]

Thank you! This helped a lot! But hey, where's ethidium bromide and UV rays? And bright orange bands of DNA?

[@Gayu_raje]

Yes.. Atlast we visualize clear bands by uv rays .. They didn't mentioned it

[@DoOrDie-kf6wh]

Me after studying biotechnology in 12

[@boyinalabcoatboyinalabcoat393]

for the blanck I use the leader + load dye. for samples of dna you need load dye + dna + enzime + enzime buffer, I am missing something?

[@jasminanil5411]

very informative . thanks a lot.

[@HayDayEveryday]

Thanks

[@salazardelmar7235]

Why do we see more bubbles at the black electrode ?

[@primermezunnn]

Modern egitimm

[@Ayça_2]

Barış hocam yollarsahoop buradayız

[@HindustanKarakshak]

Nomesh sir jindabad

[@maryamsalih3801]

how I can label the product of gel electrophoresis by which program if my gel image was taken by JPA please kindly for you recommend me thanks

[@adanqureshi2425]

What do we need the buffer for ?

[@Justlight_tr]

herkes barış hocadan gelmiş sdkfnekjgh

[@rishibhargav5714]

Watching this after reading Bio-Tech chapter...

[@Getgot-en1kv]

I don’t need sybr dye for this step right?

[@legostar8142]

Which dye do you use? And do you add the dye into the centrifuge?

[@girijasankarpanigrahi4393]

Ethirium Bromide as the movement indicator and Bromopropanal as the blue dye.

[@iclalcim]

Harika bi sey

[@krishnamani8150]

Please how to prepare agarose gel and buffer to post the vedio

[@tnemakyildiz]

hop barıs hocam