Dialysis (Protein Purification)

[@oumaymabalti1019]

in College,we study chemistry and biochemistry in French ,although I speak 3 other languages than english including french,I never liked biochemistry the way I enjoyed your lectures! I wish you were one of my professors! bravo!

[@harisbinfida6624]

im a great fan of yours...what a dedicated way to give knowledge to students..really impressed...really appreciating

[@navneetmishra3989]

One of the best professor, I have seen love u from India sir 😍😘

[@nareshbarik5384]

Thank you ❤🙏

[@chinmay2297]

We can also separate the protein from the ions in the water by using electrolytes which is oppositely charged to the ions present in the bag and also we can use electrodes setup.

[@sameirbasnet3102]

great lecture will you please explain the process of purification of HIS-tagged protein purification process.

[@r3g4rds]

how come water molecules dont pass through the membrane when held in open air? (Or do they leak out too?) Googled answer for myself: Surface tension of water in air keeps it from leaking out the small pores.

[@arunkumars6257]

Thank you so much!

[@jhonamaysuero8568]

thanks soo much well explained

[@leeschmalz6365]

You're great, thank you

[@shivanipopli6868]

sir, you are amazing.. thank you so much.

[@PhilipAlabiephraim]

Question: How is the salting out concentration calculated? because that will be very important in helping get proteins

[@anurag6205]

Thank you sir

[@tyvenghout9049]

Can we calculate the concentration at the equilibrium?

[@KoolViking3]

awesome!

[@gosia1761]

Hello, I really appreciate yours videos, thank you for all of them. I have a question, I want to know why the dialysis bag will be bigger after the dialysis. There's also something from the baker going to the bag. What is it and why ? I hope you will give me a response.

[@mpraneeth1978]

It's the water molecules diffusing from the beaker - the region of higher concentration- into the dialysis bag which is the region of lower concentration.

[@deepikadav]

can't thank you enough, Sir :)

[@dalbir435]

does dialysis would help to separate dna from proteins?

[@junczhang]

awesome

[@jayrextong3409]

is it like the teabag???? nye nye nye

[@KJKP]

I think diffusion is not covered by the 2nd law of thermodynamics. "Every energy transfer or transformation increases the entropy of the universe."

[@TheYz250fmf]

Thermodynamics is everything. You are reading the definition to close and not applying it correctly. Molecules want to increase their entropy correct? (yes) Therefore a molecule will diffuse to increase its entropy and it will be happy.

[@AKLECTURES]

Thermodynamics IS in fact everything :)

[@TheYz250fmf]

(:

[@fuhrer12345]

Your explanation is a little superficial. In fact when we deal with an open system such a human being, is quite the opposite. Most biochemical reactions in our body are indeed unfavorable (supramolecular complex), thus they tend to decrease the internal entropy. It's by reaction coupling and non covalent interactions that the molecules in our system can increase the universe's entropy. Gibbs free energy is a good measurement for this phenomenon.

[@jayrextong3409]

nadali mo hahaha ano imik hahaha nyenyenye

[@zanzan648]

How many type. Of dialysis

[@wirtsleg]

Why do cells have to use ion channels to transport the ions through the membrane but here it isn't needed? Why can't the cells also use the 2nd law of thermodynamics to have equal amounts of ion inside and outside the cell membrane?

[@Halum11]

diceperse...

[@AhmedAli-bx1vj]

i love you hhhhhhhhhhhhhhhhhhhhh