Cloning a puffball - utilizing aseptic technique in mycology

In this video we clone a puffball mushroom (Calvatia gigantea) onto MEA and BCYA agar - to purchase our living mycelium cultures and other products visit our ETSY shop Freshfungi etsy.com/shop/f... for more information visit our website freshfromthefar... MUSHLOVE

[@themyceliumnetwork]

my 2' X 4' X 11.5" flow cabinet (FFU) came in today. so happy that Gary inspired me to go as far as I have with fungi farming !! Thanks Gary (and friends), you've all help change my life for the better !

[@FreshfromtheFarmFungi]

🍄❤️

[@pinky882]

What unit did you get?

[@probiogr3863]

Fresh from the farm!! Yes!!

[@dhaval4570]

You should dip your hands in some chicken of the woods hehe, puffball is nice too :)

[@nicolas.lupano]

Hi. What medium do you used on the video? & do you have the results?, I’m interested on see the color of the mycelium on petri's dishes. Thanks

[@aiswaryapadmanabhan155]

Hi. I wanted to know whether spawns can be stored in paper bags or freezer paper? Will affect the mycelium run?

[@themyceliumnetwork]

good timing, I just found my first puffball yesterday why do you use such a large piece of the mushroom ? mine always turn green unless I use a very small piece (2mm/3 or less)

[@FreshfromtheFarmFungi]

It’s all about sterility - size doesn’t matter

[@john-smith.]

@@FreshfromtheFarmFungi You say all about being sterile....as you don't flame sterilize between culture grabs.

[@peteprzepiora3468]

So what sort of substrate do u plan on growing this out on?indoors or out?

[@FreshfromtheFarmFungi]

outdoors

[@CMZneu]

Is there a way to commercially cultivate puffballs indoors? has it been done?

[@FreshfromtheFarmFungi]

Not sure but it would be a splendid offering at the market year round!

[@phantomhawk01]

what do they taste like?

[@themyceliumnetwork]

@@MaxS535 Giant puffballs (calvatia gigantea) are actually saprobic. unsure of the species in the video, Gary needs to have a PCR test run & I am sure he knows someone for that

[@garfunkle660]

Heyy brother! So I noticed you have a vertical laminar flow hood. I've mostly just seen horizontal flow hoods being used in mycology so Im curious if youve had any issues like more contam or anything vertical one? I heard the main con is you cant stack stuff.. but any other drawbacks? Thanks

[@FreshfromtheFarmFungi]

kzbin.info/www/bejne/rGbPZqyEeN2meNU check out this video - I use both in my lab now. The vertical hood I like for doing tissue culture work and pouring plates and the horizontal hood I use for substrate inoculations

[@kathrynmoore1704]

we ate puffball for breakfast, just raw, soft, and yummy flesh that I swear is just one step away from fresh mozzarella cheese!!!

[@kathrynmoore1704]

but what willlll they grow on? that's the question to be answered :-)

[@stratocasterxyz]

PSA: Don't eat raw wild mushrooms, ever. Glad you didn't experience any I'll side effects, but this is how you give yourself food poisoning or serious GI upset.