Gel filtration chromatography in 5 minutes | Size Exclusion Chromatography | Lab Procedure

this video explains the concept of gel filtration chromatography in less that 5 minutes. Size Exclusion Chromatography concept is depicted with animation.

[@nikolaspais9029]

thank you so much i study biochemistry and this video was really helpful. keep up the good work!

[@animatedbiologywitharpan]

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[@maham671]

Me also studying biochemistry

[@NEBULAFITNESS9885]

This video was was helpful for me in microbiology , thanks sir 🙂

[@animatedbiologywitharpan]

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[@PriyankaPrasad0811]

Bro thank you for this, like genuinely. I'm currently cramming for my first biochem exam and you did in five minutes what my professor couldn't do in two weeks :P

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@shahabkhan7604]

Would be nice if you add lil bit description, written form as summary, one liner so we may revise things from that

[@generendellaquino5944]

Thank you so much for this! Keep up the good work :)

[@KCGLOBAL2024]

I love this Please don't stop now that I'm in school

[@animatedbiologywitharpan]

ok thabks for your support. please share with your friends

[@SulekhaJharimune]

Amazing work!! Keep it up 👍

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@abhiruchisah1595]

thank you .You explaine it very nicely .Im from iiser tirupati pursuing majors in biology

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

[@prakashvincent9836]

Hi your explanation is good. Just a concern is, in the 0.33 time, you tell that there is binding of proteins in gel filtration column which is wrong

[@animatedbiologywitharpan]

I think you got me wrong @ .33 sec I was talking about a general workflow of column chromatography and later pointed out that GFC is bit different than the general workflow ,it involves seiving mechanism in contrast to binding

[@Galactic_Puss]

Cool stuff man, I deal with both ionic exchange, size exclusion and ultrafiltration at work and you help me understand how the process actually works

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@patriciajimenez492]

can the elution volume be greater than the total volume of the column?

[@laibanadeem2664]

You explained really well....

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@laibanadeem2664]

@@animatedbiologywitharpan ok

[@avneetkalra1145]

thank u for making it alot easier

[@animatedbiologywitharpan]

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[@sammrayounas2681]

Suppose if exclusion range is 5 to 600 then the molecules whose size is below 5 they also trapped between the voids then these molecules also eluted with small size molecules then our reqired size protein will not be obtained is this happen or not sir????? Kindly tell me...

[@laiqaaftab8555]

what is wash in workflow

[@kimpereira4770]

This helped so much! Thank you!

[@animatedbiologywitharpan]

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[@palvoliuto6059]

thank you for your great videos. could you please answer a question for me. what type of chromatography would you recommend for filtration of a polypeptide fragment? Thank you.

[@animatedbiologywitharpan]

If the peptide fragment is previously tagged by his tag / HA tag then one can do affinity chromatography, if slight estimation of size is known then one can do gel filtration, if amino acid composition is known and it has charged residues then one can go for ion exchange as well. So it really depends on what we know about the polypeptide.

[@animatedbiologywitharpan]

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[@palvoliuto6059]

@@animatedbiologywitharpan Thanks you so much my friend! All the best!!!

[@palvoliuto6059]

@@animatedbiologywitharpan i will and i have subscribed too!!

[@hccc8782]

very useful

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@udayselva1476]

Good job brother 💓👌👍

[@animatedbiologywitharpan]

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[@syedtoufeeqahmad9023]

Thank u sir

[@debarghyapratimgupta9487]

Great.. Thank you!

[@KokoriKid1]

Great video man

[@animatedbiologywitharpan]

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[@khadojaraad8274]

Thank you ,can you put translating in to Arabic?

[@animatedbiologywitharpan]

I don't know Arabic my friend

[@Suraj_gawali]

I watching your playlist

[@animatedbiologywitharpan]

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[@Suraj_gawali]

What is your education.and what perpose of making vidio

[@Suraj_gawali]

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[@animatedbiologywitharpan]

Go to the about section in the channel you would get everything.

[@awantikatiwari8080]

What does that G represents in that beads chart

[@animatedbiologywitharpan]

I think it just the name....it dont have any significant meaning....I am not sure

[@KamleshKumar-io7hl]

i think its mean GEL

[@ethanferrer1577]

Redemption

[@nerocean09]

yoooo my slime, i know you dont know mandem like that

[@PrinessSonali]

Sir how to score well in sec C?

[@animatedbiologywitharpan]

Just by improving basic concepts and reasoning

[@PrinessSonali]

I'm very weak in chemistry. Can I still score well with certain reliable chapters only!?

[@animatedbiologywitharpan]

I think it’s possible

[@vietthangduong7629]

god blesses me pass my text. make my teacher see this cmt plz T.T

[@animatedbiologywitharpan]

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[@harmohitkaur9867]

yo can you not use the r slur thank you. if you don’t know why it’s offensive even in a scientific context, look it up.

[@prasadpitagi8787]

The explanation and animation are great, but your accent in irritative. At some point of time in this short video it drew my attention away. Speak either in Indian accent or western.

[@babangapu8189]

So is your accent british? Or like your idol sachin tendulkar