How to make Agar - Singer Instruments

[@Singerinstruments]

Hi Jamie, You can buy off of companies like Sunrise Science or Formedium Or you can simply search agar on Amazon - the exact recipe you use will vary depending on your experiment? Hope that helps!

[@Singerinstruments]

You can buy them from NEB/Sigma-Aldrich or a number of online reagent stores.

[@rblanchard]

HAH I was just looking for a recipe for a refresher for an interview, glad I stumbled upon this, hilarious. (although I would prolly measure the dry ingredients individually in case you pour too much of one)

[@Singerinstruments]

Agar must be autoclaved prior to use to avoid growth of contaminants. However there are alternatives: Gamma Irradiation Electron-beam linear acceleration Ethylene Oxide Gas Hope that helps :)

[@pinkpoodlelover101]

were can I get all this stuff.... i really need it for my science fair project

[@wafferz]

What would one do if they don't have an autoclave available?

[@Su-bn5bx]

please which one agar like agar nutrient but just defrent in one matter ?

[@abcde36924]

Don't Peptone and Glucose make reaction in the autoclave?

[@DogeUSA]

So to make agar i need 6.4 of agar...hmm.

[@dvalinswamp]

hi! what kind of antibiotic can be used for yeast ranching in homebrewing?

[@Singerinstruments]

Hi Dvalin. Thank you for your question. Although we're not experts in home brewing, you may try ampicillin or other beta-lactam antibiotics. Good luck!

[@supriokamal7072]

@@Singerinstruments hello, could you specify of how much and in what concentration the antibiotics be used?

[@emilybt93]

Is 4g Yeast Extract the same as 4% (w/v)?

[@Singerinstruments]

Hi Emily. Thanks for your question. No, 4g of anything does not equal to 4%(w/v). 4% w/v means that it's 4% weight over volume. In this case, if you dissolve 4g of Yeast Extract in 100 ml of water, it would give a 4% Yeast Extract. Hope it helps.

[@Jnthnbts]

O_o I hope he turned off the bunsen burner after adding the antibiotics. lololol.

[@lilarosa8357]

the voice in background is really disturbing

[@whity5870]

These are great instructions but most these materials arn't easy to obtain .

[@diptoahmed6058]

Disturbing tone is disgusting.