PCR - Polymerase Chain Reaction Simplified

[@tusharpathare7]

Truly simplified.. One of the best KZbin channel for learners like me

[@MEDSimplified]

Watch our latest Video on RT-PCR

[@MonaHansen]

kzbin.info/www/bejne/bZubhIeQhrx5n5Y&feature=emb_logo

[@josephw4830]

@@MonaHansen Agreed

[@vaishali3562]

@@MEDSimplified aa

[@takemein]

Here are some notes too. kzbin.info/www/bejne/mKClkqJ_fax_bMU___Polymerase Chain Reaction

[@amalal-ghory1908]

I've literally watched so many videos on this topic but this is the one that really made it stick. This video helped me so much for multiple reasons, with the first being the neat layout of the video. The second reason is relating the names of stuff to how it plays a role in the whole process, and even going as far as to explain "historical" context. Last but not least, the fact that you related the entire process to a real-life application made it a lot easier to understand how it all fits together, and it made me love it and genuinely want to understand it rather than just study it. Major thanks to you

[@isurusrimadusanka8025]

We have similar things watch this👇 kzbin.info/www/bejne/rKOqYpujrLuEl7c

[@Fresh_K]

I must confess that by far this is one of the best youtube channels I've come across.Its really #MEDSIMPLIFIED.Bless you.

[@MEDSimplified]

Thankk you for your awesome comments 😀

[@alexandrahowell3142]

HIV has an RNA genome, not DNA as you state at 6'06" of the video. For HIV, you'd first have to do reverse transcription to produce cDNA before you can do a PCR

[@kushunadkat9087]

Yes

[@fathimahashim7005]

But when this hiv virus enters the human cell, it starts to create dna for making further copies of themselves.

[@alexandrahowell3142]

The video is incorrect when it states "the blood of the patient contains the virus, and in the virus is its genetic material, the DNA". Rather, it should state, "the virus's genetic material which is a single stranded RNA molecule, is reverse transcribed within the cell it has infected, and the resulting DNA then integrates into the host cell chromosome".

[@simeonikechukwu1957]

Useful observation @Alexander Howell

[@HelloAayush]

@@alexandrahowell3142 but when hiv virus is already in macrophages then it's dna due to reverse transcription is already integrated in the macrophage cell and further in the T helper cells The viral RNA is converted to DNA in macrophages. So we can say that we will directly get the DNA integrated in the cell?🤔

[@aysha744]

Truly simplified I cant understand anymore and This topics are made me boaring But now it is very amazing Thanks a lot

[@kritikagautam1140]

So simplified and easy to understand. Brilliant Explanation. Thank you ❤

[@ilyassalmon9513]

One of the best KZbin channel for Students like me

[@nidhishreeg6292]

Thank you so much for explaining this topic in such an easy and understanding way!!

[@gayatriconventschoolbaran7538]

I m in love with ur voice it is soo calming soo soothy... Watching video of other teachers on you tube give me headache as their voice is soo noisy but listening to u is really relaxing... And ur presentation is superbb

[@oyenimeapreala6460]

ThankS to DR Rorpopor Herbal on KZbin who helps me cured my PCR God bless you DR Rorpopor Herbal his herbal medication is very infective and active

[@behappywithmanotg9379]

Really easily understandable ,very informative

[@latrellebrown7916]

Thank you! Automation Engineer here trying to move to the next level of understanding the things that I do.

[@MonaHansen]

kzbin.info/www/bejne/bZubhIeQhrx5n5Y&feature=emb_logo

[@dr.garima_gynae]

Very simple explanation of a complex topic👍👍

[@TheOiler1989]

Thanks a lot and I'll be back to learn more about this. Now I know why Kary Mullis called PCR a manufacturing process.

[@favouritethings9363]

Thank you very much for this video.It is very well explained ,simply and easy to remember

[@Emjay_Music]

You. Are . Saving. My. Semester. Thank god for you!!!

[@updown7607]

In my book temperatures mentioned in denaturation is 94 degrees Celsius and for 1 minute. In primer Annealing, temperature is 54 degrees Celsius and time duration is 2 minutes.

[@ramzanrajput8161]

one of the best youtube channels God bless you

[@nitinprakash4207]

Thank you sir for trully clearing my all doubt of this topic.

[@consolidate_23]

One min down in the video already subscribed🙌🏾, amazing explanation thank you!

[@KoToKo90cHaN]

Great video! Just wanted to note that you've mentioned two different temperatures for denaturation, 96 degrees at 4:50 and 72 degrees the rest of the video, which one is correct?

[@parv9994]

Sarah it is always 96 degrees for denaturation and 72 degrees for extension (a.k.a. elongation). He probably got confused during recording.

[@robertschlesinger1342]

A worthwhile video for all beginning biology students.

[@koushikbiswas5803]

Your voice is awesome! God gifted!

[@zafranmalikrolvikashmiri]

All med simplified videos are grt & nice

[@saikeerthanarachamadugu8171]

Your idea on flashcard is excellent

[@Khaniofficialchannel]

Super Clear voice & best explanation of PCR.Thumbs up ⚘

[@rozellas2210]

The MOST INFORMATIVE AND SIMPLIFIED VIDEO EVER ON PCR! Thanks a lot!

[@cjain646]

Great job, Very helpful, good voice. Thank you

[@MEDSimplified]

Thanks for the appreciation •°

[@cjain646]

You really have an amazing voice, the person who is drawing does a good job too.

[@sahibaikram5484]

hye, thank you so must for making this topic super easy. my English is not so good so please don,t mind. you are amazing. i am seventeen years old and i want to be a great doctor just like you. you are my top inspiration. thanks again

[@MEDSimplified]

Thank you so much for the appreciation

[@apryLwitha_why]

Hello. I'm from FLORIDA, USA. Your English is not bad at all. I understood you perfectly. I wish you much luck in your medical career as a Doctor. I'm sure you will do great things in this field.

[@basavaraj.bdoranalli9956]

U r not making it simple....u r simply making others mind simple....about a particular topic,...osm work...must appreciate..your work ..all D best...,🤘🤘

[@terrybash5194]

Thank you so much DR RORPOPOR HERBAL on KZbin you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes

[@bhinggo7255]

Thank you MEDSimplified! You have always helped me in every biochem exam! Thank you for making our student lives easier! Bless you!

[@tharindunuwangamage8688]

Good job guys

[@yrahlabog9422]

This is so helpful for my report. ❤️ Thanks a lot.

[@sandeep.v8589]

Really MED is simplified

[@RaudiRaul]

You say at 5:30 in the video that the temperature should be raised to 72 degrees Celsius for step one, Denaturation. Isn't it higher like around 95 degrees Celsius?

[@vocalistharshi]

You are blessing for us sir You explanation is so simplified, 🤧🤧really really helpful , keep making 💜💜 Thanku 🙃

[@aishwaryabhatnagar75]

thank you for this..i needed a simple and accurate definition n functioning of pcr..for an interview..thank you

[@MastermindTV]

Thank goodness i found you

[@faisalfida9929]

Best For Non Medicals😍😍

[@michaeldaveapo7207]

Your video is really relevant today for the COVID - 19 pandemic. It helps people understand the testing process of identifying a person that has COVID - 19

[@sportysbusiness]

You mean understanding that the test doesn't work? Even it's inventor said it should ever be used to test for viruses. No wonder it's 80% inaccurate, a coin toss is more accurate.

[@daisytoromanzano8865]

@@sportysbusinessThose are facts, thanks for the reminder. Kary Mullis said that you can find whatever you want, just increased the cycles. He also said never to be use to diagnosed just for lab research. Also contains E.O. In UK under a FIOA disclosure it was found that has EO( carcinogenic agent), way above safe limits. We cant put trust in any man, just Abba.

[@sunithath799]

Thank you it's really simple to understand.

[@trinaaaich3529]

Thank u so so much. Was of great help.

[@shikhakumari2212]

Very nice explanation sir👍👍👍👍

[@dhunganalaxmi8277]

Wow what a teaching style ❤❤❤

[@lihiycohen7477]

Wow thank you for this video!! Very well explained and presented. Super useful.

[@likilkumarsunkari3025]

Well explained bro

[@mychannel4397]

Very good and helpful video

[@sunithatella1554]

Thank you, 👍 it's really superb explanation

[@jyotirachchh002]

Thank you 😄🤍

[@DrReem-bm1yg]

Thank you ,, it is really really simple and clear explanation ^^

[@nehavikram007]

Finally... something made sense

[@pratikbagthariya7089]

Thank you this is the best channle for remember biology 🙏❤️👍💯

[@deemamattar517]

This video is literally amazing 😍😍

[@aqsakhan1100]

Your voice is so addictive 😶😐😅

[@profiliomongjam1556]

Thank you very much for explaining pcv in simple way

[@adabwaleobonato5561]

Very useful information and well defined...one thing mistake is here an example of Virus HIV containing DNA...🧬

[@loopeesa1823]

It's so simple and realatable!

[@lidhiyalouis1851]

Excellent presentation

[@tonywong7300]

This is an eye opening explanation. Thanks

[@satyaranjanacharya4635]

u know u voice is just 😍😍

[@pritichristian2673]

Good explanation. Thanks

[@shahbaloch4814]

Grest method of teaching easily understand and learn it thanks a lot sir 😊

[@riazkhattak527]

Thank you so much sir 😊😊

[@niks8924]

Thanks so much for this vedio...i easily understood the PCR

[@MEDSimplified]

Thank you so much

[@ravikanttyagi9633]

Is this best to detect abdominal tuberculosis

[@janicecarman3157]

Salamat pangsagot ko sa biotech 🥰

[@janicecarman3157]

Well come

[@rizwan7540]

Thank you so much sir. It's a really informative and interesting video I have really seen. May Allah accept your struggle. Aameen . Love from Rizwan 🥰

[@NisarAhmed-zd3di]

What an editing video that's amazing keep it up

[@mireillemvondo-she8884]

Thank you so much, I really appreciated the easy explanation.

[@House_of_wealth]

Thank you for this video, it really help me a lot

[@AwaisAli-xl1pu]

Really impressive thumbs up

[@tanzeelahashim8168]

Good explanation 👍👍

[@shaiestamalik5226]

Very well explained.. Please share a vedio of Rtpcr procedure for Covid-19..

[@ironshield4]

Hi, I'd like to request clarification regarding one of the points covered in the video. You say there will be 2 copies after the first cycle, then 4 after the second, and 16 after the third, then 32, 64, 128, and 512. Unless I'm missing something, or misunderstood (which is entirely possible), I think the 3rd cycle should yield 8 copies, not 16. If I'm right, then all of the subsequent values are wrong as well. Additionally, 512 would follow 256, not 128. Otherwise, it's a very helpful video, and I appreciate the work you put into it. Thanks!

[@JaswinderSingh-qi7iy]

You are right .I was searching for this comment so as to confirm that I wasn't wrong

[@yogeshlakshman8488]

Thanks to clear my doubt

[@jiniaisrat9434]

Thank you so much. I was searching an easy video. It helped me a lot.

[@crypted4527]

Please make a video on Recombinant DNA technology

[@biologywithannie]

super informative..👍

[@cse46ilanthenral.v63]

Thank you for your video

[@florashlaks]

How is the primer decided? I mean, is the base pair sequence same in all primers? What decides which primer to use?

[@karenjwoods5961]

Thank you very clear explanation..now i can understand

[@drashtibhatt6987]

I always prefer ur videos for my study bcoz which way you simplfy the topic it's just amazing

[@Roselynne1016]

Can you explain why Kary Mullis the inventor of the PCR test stated over and over that the PCR test should not be used to diagnose viruses?

[@Roselynne1016]

@John Bindini , thank you.

[@torgenxblazterzoid]

@jorge pearl Nobody has isolated and/or purified COVID-19. If you believe otherwise then post your *definitive* evidence here.

[@boilingfrog783]

@@shellysistla9037 - Hi, I'm a complete layman so please excuse any ignorance on my part. On the subject of the Covid virus, what medical conclusions would you draw from PCR positives where the cycle threshold has been set high - 40 cycles or more - when the video indicates less cycles should be performed (Professor Carl Heneghan has indicated 25 as appropriate) and the test subject is clearly asymptomatic? Is it correct to refer to such a person as a "case" and is there any realistic possibility of them being infectious? Thanks. Best regards 👍

[@alvarodias68]

@John Bindini I understand that pcr shouldn't be used to diagnose illness. But why do you want people to kiss your ass?

[@alvarodias68]

@steven atkinson Bingo!

[@abh3835]

Thank you.. Informative

[@pritammukherjee1053]

Nice video.... Pls continue ur videos regularly in youtube

[@satishgautam7234]

Hats off bro u do make med simplified

[@stevemartin4249]

One of many problems in governments using PCR for mass testing of the coronavirus - why is there no standardized number of cycles to be run to determine a positive or negative test for RNA snippets? It is as if business or political interests choose the results they want first, and then choose the number of cycles to give that result. Say it ain't so Joe.

[@danielm.1521]

Oh c'mon man...straight Malarkey! Take that mess to another comment section and quit spreading the nonsense and causing doubt. You don't like per results then go the antigen route.

[@debravictoria7452]

Diagnosis? The inventor, Kary Mullis said the PCR was of no use for diagnosis.

[@TheFelltimber]

You are confusing end time PCR with RT PCR. The latter is used for covid testing.

[@godloves9163]

@@danielm.1521 Get a life! What an ignorant response. kzbin.info/www/bejne/jIiYk4GaYqljoNk

[@kardusbox6430]

Look at the Indonesian PCR business..

[@-Shrinath]

Would like to get a video from regarding agarose gel electrophoresis, sturtevant's obsevation, Morgan's linkage theory, etc..

[@nilas8944]

This was so helpful!

[@katejbradshaw]

Well done!

[@sujitdey1717]

Quite helpful... Thank you for making this video...

[@RiazKhan-og7bf]

Good for learning in easy way but many questions can still arises here

[@MedicalBroadcast]

Indeed very well explained.

[@vedantpandhare128]

Thanks bro 👍

[@pareyiosoitara3352]

Well elaborated..thankyou

[@hadich3009]

jazakAllah hu khaira

[@Naturekasprash]

Thank you for this video ☺

[@nagatsatti8426]

Excellent .very nice thank you sir

[@aditeayah]

Thanks

[@MoyMoy-q6m]

So about the Process of PCR; Denaturation, is when the sample is heated, breaking the hydrogens bonds that holds the double-strand DNA together, resulting the separation of the double-stranded DNA; in Annealing, the sample is finally cooled down, allowing the primers to bind with the individual strands of DNA; in Extension, the primers produce more nucleotides of the short sequence of the primer and once a new DNA is completely formed, the double-strand DNA will also undergo the same steps repeatedly by 20 to 30 times, resulting the millions of copies of DNA. Correct me if I'm wrong.

[@oleglookin7007]

Thanks for the video! However, what about the rate of amplification? Other sources state this is 1-2-4-8-16-32 etc, in this video the step of 4 copies next results in 16 copies. Is it wrong or not?

[@muhammadsulaiman6023]

Very nicely explained..

[@zeeshanansari7621]

Keep it up bro🤗🤗🤗