I've literally watched so many videos on this topic but this is the one that really made it stick. This video helped me so much for multiple reasons, with the first being the neat layout of the video. The second reason is relating the names of stuff to how it plays a role in the whole process, and even going as far as to explain "historical" context. Last but not least, the fact that you related the entire process to a real-life application made it a lot easier to understand how it all fits together, and it made me love it and genuinely want to understand it rather than just study it. Major thanks to you
@isurusrimadusanka80254 жыл бұрын
We have similar things watch this👇 kzbin.info/www/bejne/rKOqYpujrLuEl7c
@tusharpathare74 жыл бұрын
Truly simplified.. One of the best KZbin channel for learners like me
Here are some notes too. kzbin.info/www/bejne/mKClkqJ_fax_bMU___Polymerase Chain Reaction
@alexandrahowell31423 жыл бұрын
HIV has an RNA genome, not DNA as you state at 6'06" of the video. For HIV, you'd first have to do reverse transcription to produce cDNA before you can do a PCR
@kushunadkat90872 жыл бұрын
Yes
@fathimahashim70052 жыл бұрын
But when this hiv virus enters the human cell, it starts to create dna for making further copies of themselves.
@alexandrahowell31422 жыл бұрын
The video is incorrect when it states "the blood of the patient contains the virus, and in the virus is its genetic material, the DNA". Rather, it should state, "the virus's genetic material which is a single stranded RNA molecule, is reverse transcribed within the cell it has infected, and the resulting DNA then integrates into the host cell chromosome".
@simeonikechukwu19572 жыл бұрын
Useful observation @Alexander Howell
@HelloAayush2 жыл бұрын
@@alexandrahowell3142 but when hiv virus is already in macrophages then it's dna due to reverse transcription is already integrated in the macrophage cell and further in the T helper cells The viral RNA is converted to DNA in macrophages. So we can say that we will directly get the DNA integrated in the cell?🤔
@Fresh_K5 жыл бұрын
I must confess that by far this is one of the best youtube channels I've come across.Its really #MEDSIMPLIFIED.Bless you.
@MEDSimplified5 жыл бұрын
Thankk you for your awesome comments 😀
@aysha7443 жыл бұрын
Truly simplified I cant understand anymore and This topics are made me boaring But now it is very amazing Thanks a lot
@latrellebrown79164 жыл бұрын
Thank you! Automation Engineer here trying to move to the next level of understanding the things that I do.
I m in love with ur voice it is soo calming soo soothy... Watching video of other teachers on you tube give me headache as their voice is soo noisy but listening to u is really relaxing... And ur presentation is superbb
@oyenimeapreala64603 жыл бұрын
ThankS to DR Rorpopor Herbal on KZbin who helps me cured my PCR God bless you DR Rorpopor Herbal his herbal medication is very infective and active
@ilyassalmon95132 жыл бұрын
One of the best KZbin channel for Students like me
@kritikagautam11402 жыл бұрын
So simplified and easy to understand. Brilliant Explanation. Thank you ❤
@nidhishreeg62923 жыл бұрын
Thank you so much for explaining this topic in such an easy and understanding way!!
@dr.garima_gynae2 жыл бұрын
Very simple explanation of a complex topic👍👍
@nitinprakash42072 жыл бұрын
Thank you sir for trully clearing my all doubt of this topic.
@updown76073 жыл бұрын
In my book temperatures mentioned in denaturation is 94 degrees Celsius and for 1 minute. In primer Annealing, temperature is 54 degrees Celsius and time duration is 2 minutes.
@saikeerthanarachamadugu81714 жыл бұрын
Your idea on flashcard is excellent
@Emjay_Music3 жыл бұрын
You. Are . Saving. My. Semester. Thank god for you!!!
@behappywithmanotg93793 жыл бұрын
Really easily understandable ,very informative
@basavaraj.bdoranalli99563 жыл бұрын
U r not making it simple....u r simply making others mind simple....about a particular topic,...osm work...must appreciate..your work ..all D best...,🤘🤘
@terrybash51943 жыл бұрын
Thank you so much DR RORPOPOR HERBAL on KZbin you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes
@koushikbiswas5803 Жыл бұрын
Your voice is awesome! God gifted!
@TheOiler19894 жыл бұрын
Thanks a lot and I'll be back to learn more about this. Now I know why Kary Mullis called PCR a manufacturing process.
@ramzanrajput81613 жыл бұрын
one of the best youtube channels God bless you
@sahibaikram54843 жыл бұрын
hye, thank you so must for making this topic super easy. my English is not so good so please don,t mind. you are amazing. i am seventeen years old and i want to be a great doctor just like you. you are my top inspiration. thanks again
@MEDSimplified3 жыл бұрын
Thank you so much for the appreciation
@apryLwitha_why2 жыл бұрын
Hello. I'm from FLORIDA, USA. Your English is not bad at all. I understood you perfectly. I wish you much luck in your medical career as a Doctor. I'm sure you will do great things in this field.
@rozellas22102 жыл бұрын
The MOST INFORMATIVE AND SIMPLIFIED VIDEO EVER ON PCR! Thanks a lot!
@favouritethings93633 жыл бұрын
Thank you very much for this video.It is very well explained ,simply and easy to remember
@bhinggo72555 жыл бұрын
Thank you MEDSimplified! You have always helped me in every biochem exam! Thank you for making our student lives easier! Bless you!
@zafranmalik89984 жыл бұрын
All med simplified videos are grt & nice
@michaeldaveapo72074 жыл бұрын
Your video is really relevant today for the COVID - 19 pandemic. It helps people understand the testing process of identifying a person that has COVID - 19
@sportysbusiness4 жыл бұрын
You mean understanding that the test doesn't work? Even it's inventor said it should ever be used to test for viruses. No wonder it's 80% inaccurate, a coin toss is more accurate.
@daisytoromanzano886511 ай бұрын
@@sportysbusinessThose are facts, thanks for the reminder. Kary Mullis said that you can find whatever you want, just increased the cycles. He also said never to be use to diagnosed just for lab research. Also contains E.O. In UK under a FIOA disclosure it was found that has EO( carcinogenic agent), way above safe limits. We cant put trust in any man, just Abba.
@sunithath7992 жыл бұрын
Thank you it's really simple to understand.
@robertschlesinger13424 жыл бұрын
A worthwhile video for all beginning biology students.
@Khaniofficialchannel4 жыл бұрын
Super Clear voice & best explanation of PCR.Thumbs up ⚘
@cjain6464 жыл бұрын
Great job, Very helpful, good voice. Thank you
@MEDSimplified4 жыл бұрын
Thanks for the appreciation •°
@cjain6464 жыл бұрын
You really have an amazing voice, the person who is drawing does a good job too.
@aishwaryabhatnagar753 жыл бұрын
thank you for this..i needed a simple and accurate definition n functioning of pcr..for an interview..thank you
@consolidate_de_dreamer3 жыл бұрын
One min down in the video already subscribed🙌🏾, amazing explanation thank you!
@yrahlabog94223 жыл бұрын
This is so helpful for my report. ❤️ Thanks a lot.
@tharindunuwangamage86883 жыл бұрын
Good job guys
@dhunganalaxmi8277 Жыл бұрын
Wow what a teaching style ❤❤❤
@vocalistharshi3 жыл бұрын
You are blessing for us sir You explanation is so simplified, 🤧🤧really really helpful , keep making 💜💜 Thanku 🙃
@deemamattar5172 жыл бұрын
This video is literally amazing 😍😍
@sandeep.v85894 жыл бұрын
Really MED is simplified
@MastermindTV3 жыл бұрын
Thank goodness i found you
@duocphamtruongtho44622 жыл бұрын
Thank you so much!
@profiliomongjam15563 жыл бұрын
Thank you very much for explaining pcv in simple way
@DrReem-bm1yg3 жыл бұрын
Thank you ,, it is really really simple and clear explanation ^^
@lihiycohen74773 жыл бұрын
Wow thank you for this video!! Very well explained and presented. Super useful.
@trinaaaich35293 жыл бұрын
Thank u so so much. Was of great help.
@sunithatella15547 ай бұрын
Thank you, 👍 it's really superb explanation
@tonywong73003 жыл бұрын
This is an eye opening explanation. Thanks
@lidhiyalouis18513 жыл бұрын
Excellent presentation
@adabwaleobonato5561 Жыл бұрын
Very useful information and well defined...one thing mistake is here an example of Virus HIV containing DNA...🧬
@shahbaloch48143 жыл бұрын
Grest method of teaching easily understand and learn it thanks a lot sir 😊
@jyotirachchh002 Жыл бұрын
Thank you 😄🤍
@RiazKhan-og7bf4 жыл бұрын
Good for learning in easy way but many questions can still arises here
@niks89245 жыл бұрын
Thanks so much for this vedio...i easily understood the PCR
@MEDSimplified5 жыл бұрын
Thank you so much
@ravikanttyagi96334 жыл бұрын
Is this best to detect abdominal tuberculosis
@shikhakumari22124 жыл бұрын
Very nice explanation sir👍👍👍👍
@likilkumarsunkari30252 жыл бұрын
Well explained bro
@bubblysnowflake58694 жыл бұрын
Thanks a lot sir.
@mireillemvondo-she88842 жыл бұрын
Thank you so much, I really appreciated the easy explanation.
@MoyMoy-q6m8 ай бұрын
So about the Process of PCR; Denaturation, is when the sample is heated, breaking the hydrogens bonds that holds the double-strand DNA together, resulting the separation of the double-stranded DNA; in Annealing, the sample is finally cooled down, allowing the primers to bind with the individual strands of DNA; in Extension, the primers produce more nucleotides of the short sequence of the primer and once a new DNA is completely formed, the double-strand DNA will also undergo the same steps repeatedly by 20 to 30 times, resulting the millions of copies of DNA. Correct me if I'm wrong.
@House_of_wealth2 жыл бұрын
Thank you for this video, it really help me a lot
@faisalfida99293 жыл бұрын
Best For Non Medicals😍😍
@nehavikram007Ай бұрын
Finally... something made sense
@loopeesa1823 Жыл бұрын
It's so simple and realatable!
@KoToKo90cHaN2 жыл бұрын
Great video! Just wanted to note that you've mentioned two different temperatures for denaturation, 96 degrees at 4:50 and 72 degrees the rest of the video, which one is correct?
@parv99942 жыл бұрын
Sarah it is always 96 degrees for denaturation and 72 degrees for extension (a.k.a. elongation). He probably got confused during recording.
@rizwan7540 Жыл бұрын
Thank you so much sir. It's a really informative and interesting video I have really seen. May Allah accept your struggle. Aameen . Love from Rizwan 🥰
@mychannel43974 жыл бұрын
Very good and helpful video
@crypted45275 жыл бұрын
Please make a video on Recombinant DNA technology
@-Shrinath Жыл бұрын
Would like to get a video from regarding agarose gel electrophoresis, sturtevant's obsevation, Morgan's linkage theory, etc..
@shaiestamalik52264 жыл бұрын
Very well explained.. Please share a vedio of Rtpcr procedure for Covid-19..
@NisarAhmed-zd3di2 жыл бұрын
What an editing video that's amazing keep it up
@ironshield43 жыл бұрын
Hi, I'd like to request clarification regarding one of the points covered in the video. You say there will be 2 copies after the first cycle, then 4 after the second, and 16 after the third, then 32, 64, 128, and 512. Unless I'm missing something, or misunderstood (which is entirely possible), I think the 3rd cycle should yield 8 copies, not 16. If I'm right, then all of the subsequent values are wrong as well. Additionally, 512 would follow 256, not 128. Otherwise, it's a very helpful video, and I appreciate the work you put into it. Thanks!
@JaswinderSingh-qi7iy3 жыл бұрын
You are right .I was searching for this comment so as to confirm that I wasn't wrong
@yogeshlakshman84883 жыл бұрын
Thanks to clear my doubt
@drashtibhatt69872 жыл бұрын
I always prefer ur videos for my study bcoz which way you simplfy the topic it's just amazing
@pritichristian26733 жыл бұрын
Good explanation. Thanks
@jiniaisrat94344 жыл бұрын
Thank you so much. I was searching an easy video. It helped me a lot.
@nazzia13 жыл бұрын
please waiting for the video on agarose gel electrophoresis. Your videos are amazing
@AwaisAli-xl1pu2 жыл бұрын
Really impressive thumbs up
@karenjwoods59613 жыл бұрын
Thank you very clear explanation..now i can understand
@Rayyanbinshad6 ай бұрын
In my book its written that in danaturation template is heated to 94 degrees and you said 72 degrees
@sameerquazi26262 жыл бұрын
96 degrees is the temperature at which DNA denatures, not 72. Once denatured aat 96, annealing of primers to strands takes place at 56 dgerees and then Taq Polymerase comes into action at 72.
@pratikbagthariya70893 жыл бұрын
Thank you this is the best channle for remember biology 🙏❤️👍💯
@aditeayah4 жыл бұрын
Thanks
@MedicalBroadcast3 жыл бұрын
Indeed very well explained.
@florashlaks2 жыл бұрын
How is the primer decided? I mean, is the base pair sequence same in all primers? What decides which primer to use?
@sturdybirdyy Жыл бұрын
Great video. But the temperature for each step could be cross checked. Plus one copy will make 2 then 4 then 8, not 16 as stated in the video. Another thing that you mentioned was that after the first cycle there is one parenteral DNA and one newly formed..it should be that there are 2 copies each with one parental strand and one newly synthesized strand..
@MunsKi2 жыл бұрын
its primary use is genetic research... not infection diagnosis, it is only later used
@tanzeelahashim81684 жыл бұрын
Good explanation 👍👍
@iftishapp623 жыл бұрын
Just wow....plz do on topic toxicology
@riazkhattak5272 жыл бұрын
Thank you so much sir 😊😊
@aqsakhan1100 Жыл бұрын
Your voice is so addictive 😶😐😅
@punithauv24474 жыл бұрын
Sir... Very wonderful teaching....
@MEDSimplified4 жыл бұрын
You are very welcome sir
@hadich30093 жыл бұрын
jazakAllah hu khaira
@satishgautam72344 жыл бұрын
Hats off bro u do make med simplified
@muhammadsulaiman60235 жыл бұрын
Very nicely explained..
@janicecarman31572 жыл бұрын
Salamat pangsagot ko sa biotech 🥰
@janicecarman31572 жыл бұрын
Well come
@dadijabutty26583 жыл бұрын
Thank you for well explained animation
@terrybash51943 жыл бұрын
Thank you so much DR RORPOPOR HERBAL on KZbin you saved my life from this deadly PCR virus, I got cured within 14daysly herpesly herpes
@pritammukherjee10535 жыл бұрын
Nice video.... Pls continue ur videos regularly in youtube
@cse46ilanthenral.v634 жыл бұрын
Thank you for your video
@satyaranjanacharya46352 жыл бұрын
u know u voice is just 😍😍
@stevemartin42493 жыл бұрын
One of many problems in governments using PCR for mass testing of the coronavirus - why is there no standardized number of cycles to be run to determine a positive or negative test for RNA snippets? It is as if business or political interests choose the results they want first, and then choose the number of cycles to give that result. Say it ain't so Joe.
@danielm.15213 жыл бұрын
Oh c'mon man...straight Malarkey! Take that mess to another comment section and quit spreading the nonsense and causing doubt. You don't like per results then go the antigen route.
@debravictoria74523 жыл бұрын
Diagnosis? The inventor, Kary Mullis said the PCR was of no use for diagnosis.
@TheFelltimber3 жыл бұрын
You are confusing end time PCR with RT PCR. The latter is used for covid testing.
@godloves91633 жыл бұрын
@@danielm.1521 Get a life! What an ignorant response. kzbin.info/www/bejne/jIiYk4GaYqljoNk