Detection of Salmonella species in Different Samples (Part-1)_A Complete Procedure (ISO 6579-1:2017)

[@khushichaturvedi3817]

Tomorrow i wll face auditor in micro lab its very helpful to me thnkyo so muchh

[@MicroChemsExperiments]

All the bestttt

[@khushichaturvedi3817]

@@MicroChemsExperiments i will try to my best

[@isayasnemera4116]

Please,give us the link for part 2 and do the video for bacillus cereus

[@For_u_m_patel]

Very on point and interesting video.

[@MicroChemsExperiments]

Much appreciated

[@simranpal452]

We have to take pre-incubated plates pf XLD or BSA?

[@Niyahgigs]

Good job, I've got a cultured plate with distinct colonies and I'll need your help in identifying the bacterium and medium used. Thank you

[@MicroChemsExperiments]

Its a long task. Its difficult to explain here. Neverthless you can subculture your colonies on different selective & differential culture media like MSA, Cetrimide Agar, XLD, SS agar, Chromogenic Coliform Agar, MRS, MYP agar, etc. After that do biochemical tests of the selected colonies for identification.

[@olabisiolatundun3696]

Well done my teacher. Please can I use this method for pharmaceutical samples?

[@MicroChemsExperiments]

Yes. You can

[@WilfridaYasinta]

Thank you so much. I try to find part 2 but cannot find. Please..

[@benedictagifty6097]

Do you have video on testing of pseudomonas Aureginosal. Please share

[@MicroChemsExperiments]

We will make it soon

[@prasadch1368]

Excellent explanation thanks 💐👍

[@MicroChemsExperiments]

Thank you

[@likeleah]

Thank you for uploading this video.. and it's really helpful and understandable too.. please do video for detection of staphylococcus spp and vibrio spp.

[@MicroChemsExperiments]

Thank you. We will make video later.

[@karvatfaisal3091]

Please make videos for v.c. v.p. and staphylococcus aureus please it's a request 👍🏼

[@SnoooooowMan]

Do you have a video for preparing xld agar plate? Because xld agar can not autoclave

[@MicroChemsExperiments]

We will recheck

[@SnoooooowMan]

Do you have a xld agar plate preparing? I don't know why my xld agar always in a liquid

[@fariyahnaseem5184]

Don't cover the lid of plate until medium is solidified N make sure the agar is completely dissolved in Duran bottle while heating in microwave This can be achieved when u see the medium is boiling clearly i.e bubbles formation.

[@Djofficials]

Thank u so much mam ...its very informative

[@MicroChemsExperiments]

You are welcome

[@StephanieEatsss]

Thank you so much, this video was very helpful

[@MicroChemsExperiments]

Thank you. Stay with us

[@angelikaplacencia8023]

Thanks! Is part 1 already uploaded?

[@MicroChemsExperiments]

Both parts are already uploaded.

[@hiruchaimae1604]

Thanks for this video ! I couldn't find Part 2, are you going to upload it ? Thanks !

[@MicroChemsExperiments]

Already uploaded. Link: kzbin.info/www/bejne/l5fThHd6q8l6g8U

[@ramdewarmanjistha8122]

hi your video is very resourceful but can we use selenite broth instead of RVS and MKTTn broth? and also another question, what is the name of this method used? thank you

[@MicroChemsExperiments]

Yes you can. Method reference is mentioned in the video title

[@RahulKumar-nu1vf]

Thanks a lot make more videos for E. Coli, and zeldahl test protein testing and show serial dilution also

[@MicroChemsExperiments]

Okay. We will make it. Stay with us.

[@SnoooooowMan]

With the exception of RVS/MKTT, any proposed salmonella enrichment broth? Thank you

[@MicroChemsExperiments]

Not at all

[@abhijitmukherjee441]

Salanite broth

[@worlujoy8490]

Is it compulsory to use all the reagents mentioned?

[@MicroChemsExperiments]

Yes

[@reddyyesubabu1327]

Good job

[@MicroChemsExperiments]

Thanks

[@sharonmamabolo9258]

Very informative. Can I use distilled instead of peptone water?

[@MicroChemsExperiments]

No. Peptone water is used for Non-selective enrichment. Non-selective enrichment can not be achieved by distilled water. So you must use Peptone Water.

[@ritikachauhanvlogs175]

Ma'am plz make a video on yeast and mould determination

[@MicroChemsExperiments]

Noted.

[@kathrinajoycemuncal9074]

Hi! I just wanna ask if it is necessary to use both RVS and MKTTn for selective enrichment? as well as XLD and BSA for Isolation? thank you so much

[@MicroChemsExperiments]

Yes. Both needed for selective broth. And you can use only XLD for isolation

[@thanith6432]

Excuse me, may I ask? What type of another sample that is easy to detect salmonella ?

[@MicroChemsExperiments]

Test poultry feces, that has the high possibility to detect salmonella

[@rambabu7652]

Very useful

[@MicroChemsExperiments]

Thank you

[@achbiotech890]

Mam, what kind of sample you r using?

[@MicroChemsExperiments]

Animal feed sample

[@BappaNondi]

Waiting for part 2.

[@MicroChemsExperiments]

We will upload soon

[@arzoosundrani1415]

Please make a video on Bacillus cereus as per ISO 7932:2004 and Staphylococci aureus as per ISO 6888-2:1999 If possible.

[@MicroChemsExperiments]

Ok we will work on it soon.

[@bolarinwaobasoge2716]

Pls why did u use 1ml of peptone in mkTTn broth and 0.1ml in RVS broth

[@MicroChemsExperiments]

Method said so to do that for the selective enrichment of Salmonella spp.

[@arunarao972]

This vedio help me alot

[@MicroChemsExperiments]

Thank you. Stay with us.

[@gustavohenrique2614]

Can you name simpler tests for detection of Salmonella with close accuracy?

[@MicroChemsExperiments]

No other simpler method to be known with close accuracy.

[@ishagoswami6182]

Mam what sample do you use?

[@claudiaalcost4378]

What are the advantages and disadvantages of this method as compared to others detection methods?

[@MicroChemsExperiments]

1. Advantage: This method involves double enrichment steps which increases detection possibility and facilitates to detect Salmonella more selectively, thus this is the more accurate method compared with other methods. 2. Disadvantage: MKTTN media, frequently, becomes not available in local market and there is no replacement media of it.

[@waleed2980]

Very info .thanks

[@MicroChemsExperiments]

It's pleasure.

[@istiakmostofa7167]

Why does solid foods need 24hrs for incubation after homogenized with buffered peptone water???..Not only for salmonella detection but also for enumeration of TPC??

[@MicroChemsExperiments]

24 hours incubation in buffered peptone water is needed for the enrichment of salmonella. But this enrichment is not applicable for TABC or TPC

[@istiakmostofa7167]

@@MicroChemsExperiments then what to do for TPC after homogenized with buffered peptone water???

[@MicroChemsExperiments]

@@istiakmostofa7167 kzbin.info/www/bejne/f523n397nM6pqtU

[@k.vaibhav5773]

What ois the name of the sample taken for the experiment?

[@MicroChemsExperiments]

Poultry Feed Sample

[@zarqaarshad411]

Can I prepare buffer peptone water from peptone

[@MicroChemsExperiments]

You can prepare according to the composition of Buffered Peptone Water.

[@niketadixit9721]

Thank

[@exclusiveshow9721]

Wauu. This is really a good video. Can anyone share with me a full document where I can find medium types used to enumerate bacteria founds in food. Please help if possible. Have a nice weekend

[@MicroChemsExperiments]

We can't provide any document. We are sorry. Thanks for your appreciation

[@fariyahnaseem5184]

Plz read bam manual

[@rehanullahofficals718]

Can please confirm 6:16 mints its 0.1 ml or 100ml its confusing

[@MicroChemsExperiments]

0.1ml (100 microliter)

[@maandancel5223]

What sample did you use??

[@MicroChemsExperiments]

Food Sample

[@SnoooooowMan]

Can we substitute XLD with SS agar

[@MicroChemsExperiments]

Yes. You can.

[@SIMRANPAL-s8m]

how much broth we have to take in the test tube?

[@MicroChemsExperiments]

10 ml

[@SIMRANPAL-s8m]

i dont have a stomachercan i use blender?@@MicroChemsExperiments

[@swuoj3893]

Thank u so much❤

[@MicroChemsExperiments]

You're welcome 😊

[@SirajulIslam-vp9gp]

Thank you....

[@MicroChemsExperiments]

You're welcome!

[@StephanieEatsss]

What was the sample used?

[@MicroChemsExperiments]

Animal feed

[@bokulhossain5771]

lot of thanks

[@MicroChemsExperiments]

It's our pleasure...

[@NONAME-yv3lq]

2:42 what sample is that??

[@MicroChemsExperiments]

food

[@aqsanaz1783]

Mam what sample?

[@dadaprincez7996]

Thank youuuu

[@MicroChemsExperiments]

My pleasure

[@hiwa_jalal]

thank a lot 😍

[@MicroChemsExperiments]

Most welome

[@shritureddy8771]

Please make part 2

[@MicroChemsExperiments]

Noted

[@surampudiraviteja5089]

Part 2?

[@MicroChemsExperiments]

kzbin.info/www/bejne/l5fThHd6q8l6g8U

[@DeFiDebbie]

Do we need to make 225ml of BPW in the non-selective isolation stage? Why not less than that?

[@fariyahnaseem5184]

Refrence?

[@MicroChemsExperiments]

Iso 6579

[@moses3290]

Good video but the method is time consuming.

[@MicroChemsExperiments]

Yes, you are right

[@ajitpatil2153]

E-coli o157 plzz selective Agar name

[@MicroChemsExperiments]

HiCrome EC O157:H7 Selective Agar Base, Himedia

[@ajitpatil2153]

@@MicroChemsExperiments thank you dear

[@MicroChemsExperiments]

@@ajitpatil2153 You are most welcome

[@ajitpatil2153]

@@MicroChemsExperiments hicrome coliform Agar ???????

[@tannukumari8336]

name of sample ,?

[@MicroChemsExperiments]

Animal Feed

[@gaurvibijalwan4534]

Everything is okay, except the narration. The speech is not clear. Please work upon that. Rest, I love your channel.

[@MicroChemsExperiments]

Thank you for your observations. We will try to improve.