PCR and RT-PCR Test Explained | Tamil

What is PCR Test? Tamil
What is Polymerase Chain Reaction? Tamil
How PCR works
Difference between PCR and RT-PCR
What is PCR Testing for Viruses
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This video explains in detail about PCR and RT-PCR tests in Tamil.
PCR stands for Polymerase Chain Reaction. PCR is a major molecular biological technique used to amplify DNA. Dr. Kary B Mullis, an American Biochemist invented PCR.
Applications of PCR: In the diagnostic field, Biomedical Research, Forensic Sciences, Parenting disputes, etc.
Types of PCR: Real-time quantitative PCR, Reverse transcriptase RT-PCR, Nested PCR, Hot start PCR, Multiplex PCR etc. Agarose gel Electrophoresis technique is used to visualize the amplified DNA in PCR.
Reverse Transcription Polymerase Chain Reaction (RT-PCR) is used to detect and amplify Viral RNAs. RT-PCR is an accurate method than a rapid kit test for viruses.
Requirements for PCR test: Template DNA, Primers, Taq Polymerase enzyme, Nucleotide bases, Buffer, and Thermal Cycler.
PCR cycles: A typical PCR takes place in an automated thermal cycler machine consisting of a series of 20-40 repeated cycles with consistent temperature changes. In each cycle, there are three steps called the initial denaturation (94°C-98°C), annealing (50°C-65°C), and extension (72°C)
Sources Credit:
• Gel Electrophoresis An...
pixabay.com/
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Disclaimer: All the information mentioned in this video is only for educational purposes and doesn't advocate any other consult or opinion. So consult your doctor for any procedures and clear knowledge.
பயனுள்ள மருத்துவ மற்றும் அறிவியல் விஞ்ஞானத்தை எளிய தமிழில் மக்களுக்கு சேர்ப்பது எங்களின் நோக்கம். இந்த வீடியோவை உங்கள் நண்பர்களுடன் பகிரவும். MEDICAL SCIENCE VIDEOS - தமிழில் by Science Insights

[@scienceinsights]

Unlike PCR, in RT-PCR nucleic acids will be detected in Real-time using probes.

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@@StatusixinKirubhakaran Doctorate in Medical Microbiology

[@StatusixinKirubhakaran]

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[@abilootis2762]

Thank u mam

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Mam, a very small amount of DNA is adequate for amplification by PCR being highly sensitive...But why do some patients who were actually affected by covid still got negative results???Pls clear my doubt mam

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மிகவும் அருமையான விளக்கம். இதேபோன்ற பதிவுகள் தொடரட்டும்....

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⁰

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நான் ரொம்ப நாளா தெரியணும் ஒன்னு நினைச்ச ஒரு விஷயத்தை இன்னைக்கு இந்த வீடியோ மூலம் தெரிஞ்சுகிட்ட ரொம்ப நன்றி விளக்கம் மிகவும் அருமை

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மிகுந்த உபயோகமானது நன்றி

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Why a 7 month baby with suffocation has died during a PCR test _____ Can a 7 month baby be tested for Pcr while having shortness of breath?

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இதைவிட எளிமையாக யாரும் சொல்ல முடியாது நன்றி மேடம்

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நன்றி

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