pET21: Plasmid, Expression Vector, T7 Promoter

[@vtrindade]

Thank you for this video! Helped me a lot ! If I want to add a fusion protein so that I can increase the solubility of my protein, is the fusion protein inserted into the vector along with my gene? I got lost in the order of the plasmid construction steps..

[@Dr.DNA-Primer]

Hi, Thanks for the nice comment You choose the Expression Vector with various tags (For example GST tag) to increase the solubility and stability of your protein. I suggest expression vector with GST tag may serve your purpose.

[@vtrindade]

@@Dr.DNA-Primer thank you very much !

[@vijjumahankali4297]

Such as a wonderful explanation sir thank you ❤🎉😊

[@Dr.DNA-Primer]

Welcome