Please robot, take my job!

[@saugesmith2760]

This guy is almost singlehandedly reinvigorating my interest in Biology

[@wardfiesel1332]

The man created mine XD

[@sontapaa11jokulainen94]

@@wardfiesel1332 same here

[@zoutig.4725]

Almost?

[@p_h261]

soo true, he created mine

[@spencermanyet5336]

He is for me!

[@waterunderthebridge7950]

I felt the opening problem in my pipette-scarred fingers, the sheer collective lifetime lost to mindless pipetting is just wow

[@waterunderthebridge7950]

Always room for improvement tho, maybe our (grand)children will one day be able to skip some of the hassle of endless pipetting in standard protocols 😉

[@thethoughtemporium]

@@AliveInTheLab Robotic bio is the future. I hate 96 well plates and it takes a fraction of the time to write a quick program and run it with this than it does to do a whole plate by hand. And if you ever have to run the same thing again, just grab the file and you're good to go. This and robotic remote labs are the future

[@rndargis0]

@@thethoughtemporium and that's why I studied in robotics . Robotics automatisation is inevitable after a certain scale, and even if we got to the point that you can make anything in a GUI , you still need someone to program the robot for adding new components and protocols.

[@robertschnobert9090]

@@thethoughtemporium I love you

[@michaelclark4876]

I, and the thumb arthritis I now have from my lifetime of pipetting, quite literally feel your pain.

[@KW-jj9uy]

I can hardly believe it... That is the Vanguard microscope i sold a year ago with micro-manipulators. At the time, i was interested in making cloning equipment. I hope the microscope can be fully restored and made extra nice. the glass needs alignment and cleaning.

[@appc23]

i suggest stabilizing the table, the googly eyes are making the entire thing wobble

[@surfmanfish]

The amount of shake from the robot arm moving is jarring.

[@SUDeltaS]

Epoxy it to a 100kg granite countertop

[@Kenionatus]

Was noticing that as well and wondering whether the base of the robot would stable enough to not move (significantly) when properly attached to a stable and rigid surface.

[@suryanshtagore7180]

maybe its a feature not a bug

[@jozefvindis4926]

Agree, I would sugest sturdier / heavier table and/or limit accelerations for the moves if its possible.

[@quillclock]

I love it when art and science rub together. they dont often hang out but when they do its always entertaining

[@StainlessHelena]

and they were labmates

[@joelkulesha8284]

If you like science and art mingling then looking into early electronic music is probably the coolest thing imo. Tons of really smart people in electrical, mechanical, and electro acoustic engineering putting all of the cutting edge knowledge of the time into making music. People like stockhausen using WW2 electrical test equipment to make music. Or people like Delia Derbyshire using magnetic tape in new and experimental ways in a time when there was maybe 40 tape machines in the UK total. Or the synth makers like bob Moog, don buchla, Serg, etc. All brilliant people making the building blocks that all modern synths are built on. Hell, all of modular synthesis on its own is super cool, even today. Like there is a module for modular synths call the squid axon and it's a simulation of how real squid axons work, but applied to a musical context. Its all really cool stuff, well worth looking into! (Don buchla is probably the best place to start for that mix of art and science, plenty of documentaries about him too)

[@abadlydrawnsnowman1648]

um... Architecture?

[@Redshift666]

Art is science.

[@TheFalseJared]

@@Redshift666 Both the words "art" and "science" are so loosely defined that the sentance "Art is science" is meaningless

[@Clockworkbio]

Wow--you know you're on the really cool side of KZbin when you're getting Opentrons product placement. I interviewed there a few years back and have been excited for them ever since. Glad to see they're killing it and sponsoring some of my favorite folks out here!

[@DeviRuto]

I'm thinking you should bolt the robot's frame or the workbench to the wall. Those vibrations are scary.

[@recklessroges]

Counter point: Or the mechanical vibrations introduced by the motors might be introducing fluid mechanics to the samples that could lead to a discovery?

[@johannesvahlkvist]

@@recklessroges breaking news: bakteria filled liquid sloshes when moved

[@chemieju6305]

Also maybe do the old 3d printer trick and set it onto a big stone/concrete slab

[@oldkingcrow777]

​@@chemieju6305 or do the oldest trick in the book, and put something squishy underneath lol

[@pyronac1]

i wish there was a hackerspace near me. but damn yours would be my dream.

[@truestopguardatruestop164]

Same

[@asailijhijr]

I didn't think there was one in my area, but then I found out it was inside my local library (which I hadn't been to in many years).

[@pyronac1]

@Internet Guidance actually i have indeed been thinking about that myself. unfortunately it is not something i can afford right now. time and money are an issue.

[@savage101.]

My local hackerspace is ruined by the elitist mentalities of the people that have been there longer and became "master instructors" somehow, even though they struggle to hold a .01 tolerance. My 10+ years of aircraft lathe manufacturing experience meant nothing to them. 🤷‍♂️

[@Verlisify]

"It even made a reminder to like the video" I thought that was a submarine...

[@Durtle02]

How come I always see you in random comment sections lmao

[@blendpinexus1416]

same, i thought it was a submarine

[@iluvpwny7565]

8:20 I thought it's a submarine until he said it's a thumb up

[@matejlieskovsky9625]

That is a subliminal instruction to subscribe! XD

[@KX36]

i thought it was a 2D Benchy

[@tankart3645]

I thought he was showing us the middle finger.

[@marianh69]

your videos literally made me choose a biology career.

[@truestopguardatruestop164]

I am going to choose it too. I still need few years to start studying the specific bio engineer. Let me know if at school they teach things he do or not.

[@joshgilligan5466]

This is very cool to see! We use an Eppendorf liquid handling robot in our lab and often the GUI is extremely frustrating and limiting, as a handy tip for agar plates, older ones loose a little moisture and things stick better so you might not need a blowout step. You could also let your plates dry out a little when you get a decent hood and that will help also.

[@fureversalty]

You're now like the NileRed of Biology, can't wait for the new projects!

[@Ithirahad]

Bacterial pigment red. BioRed?

[@namAehT]

Funny enough, NileRed is also moving to a new lab. I actually assumed at the start of the video that they were moving into the same lab, since they have overlapping needs and have collaborated before.

[@Altair2000]

i’m being taught how to do covid tests at walgreens. please don’t let your boy steal my job

[@LukeDeGraff1]

Boy?

[@Altair2000]

@@LukeDeGraff1 the robot

[@n.g.s1mple29]

Its too late, your gonna be homeless soon

[@Altair2000]

@@n.g.s1mple29 goddamn it

[@virutech32]

@@Altair2000 if it makes u feel better so will everyone else so at least u wont be lonely:)

[@kentvandervelden]

Labs in transition between companies often scrap the old equipment. The computers are generally removed or the instruments are custom, so reverse engineering needed if the manufacturer will not help. I've visited a few of these labs to help the new owners repurpose or salvage equipment. The quality (and original purchase price) of the equipment is generally high. Not sure how how to locate them in general, but lab robots, especially plate and liquid handlers, would be typical finds. Someone will probably know someone...

[@mathewjoshy9774]

How can one follow that locally ?

[@fenice319]

0:15 MY EARS, I was not expecting that spike in volume

[@antonelloprodomo1473]

I got some This old Tony vibes

[@DehimVerveen]

I got medlife crisis vibes: If it ducks like a quack!

[@xusdk]

@@DehimVerveen i came here looking for this comment!

[@goodboiadvsp3297]

"I made Minecraft Steve IRL out of living voxels" - title of your next video

[@brainletmong6302]

2038: "I taught my Alpha-7 Sentience Class AI how to genetically edit a perfect catgirl clone to my tastes"

[@_cyber_cookie_]

now this, this genius

[@virutech32]

ah the obligatory catgirl comment, very nice👍🏽

[@Smitchprime]

Ur profil photo got me for a sec

[@_cyber_cookie_]

might as well make some foxgirls while we're at it

[@dargtagnan3696]

Wdym 2038, in Japan you can legally investigate that RIGHT NOW

[@azuredragonofnether5433]

Stereotypical Scientists of the Future: I cloned a human! This Scientist: I taught a robot draw a Creeper Face with bacteria.

[@StopChangingUsernamesYouTube]

"What is my purpose?" "You are a pixel." "Oh my god."

[@saucelessbones5872]

Imagine 3d printing an entire human skeleton with your own DNA , so when you need a bone replacement you have spear part

[@jskratnyarlathotep8411]

biospears \0/

[@azertyuiop432]

I'd prefer to grow a brainless clone of myself for organ harvesting

[@benjaminbarr8714]

Human bones have dense blood vessels which move lots of blood through our bones.. yours is a novel idea but practically impossible.

[@noeljonsson3578]

Spear?

[@bobjonesisthebestastro]

SPEAR PART SPEAR PART?

[@TheLophius]

Would it be possible to use something like a tattoo needle to "tattoo" bacteria in to agar to limit splash back and make dots super small?

[@fred_e]

unklikely

[@sjege]

Putting bacteria into agar creates a different kinds of problems, mainly that it cant exchange gasses. If it is aerobic it will most likely just not grow and anaerobic will have limited growth.

[@sirareus]

One of the coolest "Subscribe" signs I have ever seen. The lab looks great.

[@relicvault1515]

Everybody’s gangster until the drawing comes to life.

[@Zircuitz]

"Most errors occur between the keyboard and the chair" That cracked me up a little 😂

[@feliksjakubow8372]

I am very glad that this is the reason for the break in posting videos, and I am extremely glad you're back!

[@BloodAsp]

You should definatly make a bio QR code to your webpage!

[@sacation6057]

YES PLEASE! it should be doable when looking at the link pixelart, and then send give them away as promotion to labratories, no bio enthausiast can resist the urge to scan a biological QR code righy?

[@brown56765]

I'm very excited for more progress on the neuron project!

[@Kjackhammer]

What if you guys gentically engineered coffee beans, or tea leaves to make ingesters drowsy? That would be hilarious for a prank!

[@Elenthiriel]

FiiiiiiiiINALLY a channel for what i love with high quality production

[@jim2725]

I could recognize that Kurtzgezagt poster from a mile away, I have the same one!

[@wyattc6572]

i knew i recognized the style lol

[@pollosama1634]

Nice work! Just two things that could be interesting for you. Have you thought of 3d printing a petri-dish sized plate with tiny spikes to create small wells on your agar while it solidifies? this could solve the droplet leaking problem. Also, people tend to use alcool at 70% to kill bacteria instead of 100%. The problem with 100% is that it can make some bacteria sporulate as a defence mechanism. This tends to happen less with 70%.

[@rustycobalt5072]

If ya add a lot of weight near the bottom of that table to get rid of a lot of vibration/movement effects Edit: probably...

[@dr.fistingstein1566]

BROOOOOOOOOOOOOOOOOO.... super excited to see the new lab and workshop in action, in the future. Bring on the science!

[@Benivator2]

I'm happy to see you improved your tools. People really dont appreciate the importance of a consistent workspace and reliable tools.

[@svartmetall48]

Our Beckman i7 protocols were used for covid sample prep - all in one sample prep using multichannel manifolds and a span 8 to allow both whole plate pipetting and single well. I use the i7 for mass spectrometry sample preparation, cellular extraction etc. the liquid handlers are a gamechanger. For entire protocols though, the cellario setups from HighRes are where its at.

[@benmcreynolds8581]

That Link pixel art 🎨 one was by far the best. It was crisp and didn't have any splashes around the other areas

[@openlink9958]

4:43 as a soon to be engineering student, I feel jelous but at the same time: "YES! finally stuff I have experience with..."

[@michaelclark4876]

Someone else may have mentioned this, but Alexander Fleming was an early microbial artist. Back then he had to use different bacteria to get different colors, and they grew at different rates and growth patterns, and their interactions as they grew were important for getting the right effect. This was likely an important preparation that made chance favor his prepared mind when he noticed how that Penicillium mold contaminant produced something that killed Staphylococcus on his plates.

[@alexandrumarzenco6998]

r/3dprinting and every 3d printer owner in the universe: "LEVEL YOUR BED... i mean LEVEL YOUR AGAR"

[@Itssmial_Ova]

WOW! Congradulations on your new lab man! Definately has a less jury-rigged bio-lab vibe (To be honest I loved that vibe) But stolked you've got a new hangout.

[@the_undead]

"to be fair this is not something the robot was ever intended for" I'm going to correct you there, this is something that the designers of this robot probably never their creation would be used to do

[@benjaminbroudy2982]

Your videos have inspired me to want to pursue a major in microbiology. I find it so fascinating that we can like keep little microscopic bacteria alive in a dish

[@The.Talent]

I thought I recalled this machine from pre-COVID, so when this was your first video on it, I was a little surprised. Glad you’ve finally got a space to use it!

[@s33wagz]

I dont come to your channel enough but i've been subbed for awhile and dig every video i watch. I think I've recommended your channel more times than the amount of your videos I've seen haha. Excited to hear your plans and see what the channel has become. Truly awesome content man!

[@sickboi11111]

More spider yeast please! I want to see a space elevator grow out of the sky!

[@RyuuBaka]

Can't wait to see your future projects!

[@creature2322]

Keep it up dude, can't wait to see your further progress in the near future. 🙂

[@Koeng101]

You can solve the tip-agar problem by slowly dispensing above the agar, making a droplet on the tip, and then poking the agar. The droplet should then absorb into the agar. You get a little hole in the agar where the tip poked it (the droplet still makes a little circle), but other than that, it is a reliable method for plating that I use with my p20M to do high throughput transformations.

[@LuxxyOfficial]

new lab looks lit. looking forward to the new stuff you couldn't have done before.

[@Smokkedandslammed]

Dude! What a bad ass upgrade! Your killing it brother!

[@VeryHighPriest]

okay but I'd litterally die to see a "a boy and his atom" style stop motion animation with the bacteria paint robot

[@VitaminTheG]

can't wait to see the mountains you'll move! Keep up the great work and congratulations on achieving the dream for diybio/biohacking!

[@tdarkwolf2434]

Nice, I love the new space. I'm exited to see some of your awesome work out of the new lab!

[@FullModernAlchemist]

Holy moly dude I can’t believe that new lab. So amazing to see how far you’ve come.

[@Veptis]

That lapspace looks great. But it kinda takes a way the "you can do at home in your hobbyist space". Or all the homebuilt tools. The professional options do have merit. Excited to what's coming next tho. Having the liquid be level is a big task in lithography when you spread the photo resist. But it's a much smaller scale than bacteria medium. Perhaps a similar approach works (spinning it really fast)

[@gudadada]

I feel like this channel is kinda the NileRed of biology, it's great.

[@AMTunLimited]

> Crown jewel of the lab > Immediately puts googly eyes on it...

[@hullinstruments]

Congratulations man! Nothing better than watching someone with the knowledge and drive to push things forward and help everyone learn! Folks like yourself truly deserve the tooling and the facilities to allow them their activities. Especially since it benefits so many people! For The huge number of us who came to your Channel long ago… A lot of us are interested in the optical, laser, electronic, and radiation stuff that brought us here. Hopefully much more of that in the future 🙏 Some of us just aren’t cut out for biology, and were glad to leave it behind in college or high school

[@Sleighbells96]

Hey I noticed that there was a fair amount of vibration in the solution, if that becomes an issue you might want to find a more rigid table to put it on or what I found works really well for 3d printers is to put some of those paver stones you can find at any home improvement store and put them underneath the corners. I don’t know much about biology so I can’t say if the vibrations will hurt anything, but I do know 3d printers so if they do hopefully that helps!

[@thethoughtemporium]

The table is going to be bolted to the wall. The tables in the original lab and temporary lab were free floating, but the new one is rigid so that doesn't happen

[@fitingsthdown]

dude..when you were showing the results of the work on that spinning thing.... at the end of that shot when you flip back to the machine dipping the pipet...the image looked like it was spiraling slightly. i then looked at a poster on my wall and the same illusion was taking place. it stayed that way for a good 30 seconds. cool.

[@geoffdavids7647]

3 minutes ago?! Last time I was this early cavemen were still discovering fire

[@bottlekruiser]

It would be pretty cool to run a cellular automaton like GoL on actual cell cultures, if such a thing is even possible. Robot could set up initial conditions really precisely.

[@Silver-xp3es]

Loving the new lab!

[@ransombot]

If you have those transducers from the sonoluminescence video still might try using those to agitate the agar. If it's anything like cement should work well to level it out before you print.

[@zu.kki8]

8:52 “I lied to it” ~betrayed robot noises~

[@AA-gl1dr]

Thank you so much. I love your channel more than nearly all other channels. If I wasn’t funding my own lab I would gladly donate to your patreon. Still might. Please never stop.

[@MarcelRobitaille]

You should consider turning down the acceleration to prevent it shaking your table so much. You can change this in /var/data/robot_settings.json. Brought mine down by an order of magnitude and it helped a bunch. I have no idea why Opentrons sets it so high by default.

[@anesthetized7053]

dude that minecraft creeper could literally be sold as a poster print, its soo cool. kind of has a outerspace / scifi feel to it.

[@ugabuga4825]

That you fixed your lactose intolerance was a vety strong symbol that genetic disease will be cureable one day and that genetherapy works in human. I have connective tissue disease and hope that gene editing/crispr will help people like me and people with gentic disease in general. Im so thankfull that you do this channel and wish you the best.

[@andrewchapman2039]

Bioprinting would be amazing, but I'm sat here astonished by the practicality of a CNC pipette machine.

[@SciFrank38]

The complement system poster from Kdagjhdsajlhgdsj (kurzgesagt) so cool :D

[@Onio_Saiyan]

Of course the robot has googly eyes on it. He’s a little friend! He needs a friendly face!

[@tokalanz]

Welcome back. I've missed your videos and I'm looking forward to see more.

[@pjz7088]

The amount of patience. Good lord.

[@pi_miners2153]

A Thought Emporium Upload? Today Is A Good Day!

[@GiomDe]

cant believe i just randomly typed in my fav youtube scientist who never uploads to find out hes building my dream lab D'; its also the first of your videos ive watched ever since studying bio myself, which makes me all the more jelly of that robot you got. Also if you get the bioprinting working you might be one of the sole people on earth to posess a 3D printed bone-knife. That'll surely piss off the japanese knife making guy xd

[@nickmagrick7702]

that link and jellyfish is pretty cool. You should be proud of that its original art im pretty certain

[@KeKe-bv8qv]

The googly eyes are a great touch.

[@angelindenile]

Just the glasses and the mustache. That's all the bot needs.

[@JudgementJury]

I have found my new favorite content creator because holy fuck I'm going into Biotech and I absolutely adore biology and genetics. Update Googly eyes

[@muuuuuud]

Congratulation on the new work space, can't wait to see what it will allow you to do in terms of research.

[@mozkitolife5437]

Hey man, very excited to see that set up. Jealous is a huge understatement. Congrats on the -80. It's super compact (I've never seen them that small). You messaged me about getting one and I thought it was going to be the huge units I have in my lab but that's a really cool and space(spine)-saving solution. A suggestion on the meniscus issue: can you set agar in a large, shallow tray and use the petri dishes as cookie cutters to extract the inner, flat areas? I know agar is fragile but if you get a very shallow tray, lay all the petri dishes on top, press them down with a board, then flip the entire thing (like the baking a cake trick), you might get really flat agar canvases to work with. You might need to add an air hole to the petri dishes with a soldering iron (they work great on polystyrene). And like others have said, deal with that wobble :) Can't wait for what your channel has in store.

[@Boop__Doop]

We have to hire painting feeder's now

[@not_anson_]

This robot is amazing I’m a student studying genetic testing and one of the things I hate the most is dna extraction it’s just so tedious, thanks to this robot I can get to the good part which is reading the dna

[@scottbruner9987]

The content we are used to is amazing. So with the new digs, wow. Sky's the limit! Carry on, 🧪!

[@suryanshtagore7180]

i have never ever been so exited for someone else ever before!!!!!!...You goooooooooooo boiiiiiiiiiiiiiiiiiii

[@zephyr9450]

Your new lab looks amazing.

[@kylebowles9820]

Nice new lab!! Can't wait to see what you do with it

[@cccircuit8296]

Yay! 🎉 You finally got a proper setup! This should relieve a lot of difficulty so you can focus on your work and the videos, I can't wait to see more, maybe regular (?) content from you. You're Awesome! 😊

[@JB-dm6zt]

I think the table the machine is on is a bit wobbly. Loved the vid though, glad to see you back :)

[@aliceweirdopants4297]

you just continue to be my personal definition of sci fi. please dont over produce, u are just too awesome

[@cvoisineaddis]

6:33 seems you need a sturdier table.

[@eugenevarbanets3898]

15:50 "printing myself a scull or a set of antlers" whats next? a pizza wedge instead of hands ;)

[@kizinaki]

Realllly making me wanna switch fields with your videos

[@mh6276]

That beginning sound effect was WAY to loud. It scared me.

[@SpencerPaire]

That subscribe push is amazing! So clean!

[@coreytaylor5386]

it looks like the robot's hardware is nearly identical to a 3D printer minus the hot end for melting plastic and a heating bed and just an air tight enclosure. if thats true, the capabilities of that thing is going to be really impressive in what you can get that thing to do

[@GaussianBluff]

Lab goals tbh

[@acompletelynormalhuman6392]

I love how the first thing he does with very expensive lab equipment is stick googly eyes on it