Sub-culturing cells || Cell culture basics

Sub culturing (aka passaging), is the removal of the medium and transfer
of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.
The growth of cells in culture proceeds from the lag phase following seeding to the log phase, where the cells proliferate exponentially. When the cells in adherent cultures occupy all the available substrate and have no room left for expansion, or when the cells in suspension cultures exceed the capacity of the medium to support further growth, cell proliferation is greatly reduced or ceases entirely . To keep them at an optimal density for continued growth and to stimulate further proliferation, the culture has to divided and fresh medium supplied.

[@katherineramirez8941]

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[@animatedbiologywitharpan]

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[@georgistan]

Really good video! I have watched some before but this one provides the most clear information. Thank you!

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[@Natasha.narkar]

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[@pritammaitra4072]

So much helpful and excellent presentation

[@jacqueonassismec3430]

well explained, thank you very much.

[@animatedbiologywitharpan]

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[@dineshsaajanbhandari5736]

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[@TALKyLIKEENGLISH]

Well-explained, thanks 😊

[@misbashaikh7646]

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[@animatedbiologywitharpan]

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[@MUHAMMADALIARIFbba-fa19-vm4hp]

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[@cartikey4687]

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[@animatedbiologywitharpan]

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[@animatedbiologywitharpan]

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[@meghanak9898]

Thanks!

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

[@anupamat.k.4069]

Nice presentation thank u , so helpful ..upload more videos ..pls

[@animatedbiologywitharpan]

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[@forbesavila8827]

Do you use BioRender? Nice video man best of luck.

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[@akankshakadukar7046]

Thank uhh fr such a great explanation

[@animatedbiologywitharpan]

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[@viditdeshpande3259]

thankyou sir

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

[@sara97979]

how to know the split ratio that I need to use to seed the cells?

[@Ankitsingh-qf6el]

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[@animatedbiologywitharpan]

Yes I am there

[@xieruiyun5633]

Hello,thanks for your amazing video.It's really useful in my experiment.But can you teach us about MTT assay this experiment?

[@animatedbiologywitharpan]

Thanks for your suggestion....I will upload a video on MTT assay soon.

[@maheksharan5175]

at what rpm do we centrifuge

[@animatedbiologywitharpan]

200-300 rpm is good to precipitate cells

[@MuhammadAhsan-fy4cr]

Sir primary secondary sub culture ki video khaaan hain ? Ye tu btain ..bht aala parha rai ap ...

[@animatedbiologywitharpan]

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[@animatedbiologywitharpan]

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[@animatedbiologywitharpan]

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[@yy7w]

ا

[@animatedbiologywitharpan]

Follow me on instagram and facebook My social media handle is @animatedbiologywitharpan You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me