PCR types in Hindi |colony PCR, nested PCR, inverse PCR, real-time PCR, rt PCR

[@monikasingh6839]

Beautifully explained

[@shomusbiologyofficial]

Thank you

[@Aendrila231]

You are the best Sir! I salute your efforts towards teaching us with such integrity❤

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@thecerebrum.]

You’re the best sir!!!!!!!! You should get prize in biology 🧬

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@anjaligupta6180]

yes..he should be awarded

[@nidakhan8314]

Yes indeed itny difficult topics itny asan tareqy sy explain kiye ha

[@madanmohanmishra2759]

He has csir net qualified

[@manishakhewadia6133]

Awesome.....thnks....aapke videos bhut achhe hote h nd helpful hote h👏👏

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@urbashisharma3893]

Sir I love you soo much ...mne bahut wait Liya ki kB aap hindi m daaloge...am soooooo happy ..thanks

[@shomusbiologyofficial]

Glad I can make you happy to learn

[@drshinwarishinwari1253]

I like your Hindi you explained very well in Hindi 😍👍🏻

[@rishtanandinisingh4526]

THANK YOU SO MUCH SIR ! U R THE BEST BIOLOGY TEACHER ON KZbin

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@kidscartoon6405]

Very nice explanation sir

[@shomusbiologyofficial]

You're welcome

[@sanjayagarwal2339]

Sir you are amazing ...thank you sir

[@swatichauhan2531]

Excellent job sir👍👍👍 You have good technical knowledge .You explained PCR in such simple way that I am finding easy to learn all this.Your hindi too is good.

[@shomusbiologyofficial]

Thank You're very much for appreciating my efforts

[@ananditajha6718]

Really superb... Mr Suman. God bless you 😇

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@avinashkumar-ow7pq]

Thank you for explaining the things in Hindi

[@shomusbiologyofficial]

You're welcome

[@shadabkarim216]

It was explained superbly❤❤❤

[@narottamsahu1435]

Thank you sir very nice explain in hindi ,, 🙏🙏

[@aayushipathak6666]

Thankyou so much sir 🙂

[@shomusbiologyofficial]

You're welcome

[@masroorkhalil9655]

sir your hindhi is best ever

[@shomusbiologyofficial]

Thank you

[@masroorkhalil9655]

Shomu's Biology si pls make a video on how to write thesis in scientific writing pharaphases in hindhi pls pls pls sir

[@ayushisamriyar4732]

Very well explanation sir.. Clearly understood by anyone.. Your way of explanation is so clear nd smooth... Thnku so much sir...

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@ayushisamriyar4732]

@@shomusbiologyofficial sure sir..

[@aboyfromhills2559]

First to watch. Thanks for every videos sir. I am going through your videos all my semesters. ♥️

[@shomusbiologyofficial]

Glad you liked my lectures

[@sidrakhan8589]

Your Hindi/,Urdu video are much more good for understanding. Even though English are also v well elobarated. What about starting a separate new channel of shomu biology Hindi

[@shomusbiologyofficial]

Thank you

[@samgaming91632]

Best of the best

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sayantansaha53]

Greatest ever explanation Sir.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sayantansaha53]

Welcome sir

[@deepikamahto8039]

Nice explanation

[@shomusbiologyofficial]

Thank you

[@sushreemonali36]

Salute to you 👍👍👍🙏🙏🙏🙏🙏

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@snehak7582]

I am so used to your english videos that its weird to watch you explaining in hindi!😂

[@totalgaming5792]

Yas

[@payelbasu1321]

Sir your teaching is just incomparable to anyone ....please can U explain ITS region Nd related topics

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@amanagrahari12]

too good sir

[@shomusbiologyofficial]

Thank you

[@deepikadev9361]

thanks sir hindi vedio bnaane ke liye

[@gauriargulwar6035]

U speck very nice hindi,

[@shomusbiologyofficial]

Thank you

[@SunilaFeri]

Excellent efforts...special thanks❤❤❤ i have one question, where does the primer attach the DNA strand in reverse PCR??? Because when known sequence is in mid, primer is attached at its sides and continues to add nucleotides further next to itself in the direction of known sequence but now the known sequence is at ends so how will primer be attached???

[@xubivet3295]

Sir itna easy bana diya❤️

[@shomusbiologyofficial]

You're welcome

[@sandeshhulgunde5709]

Nice hindi sir

[@shomusbiologyofficial]

Thank you

[@pabitraamadhurima6960]

In the case of inverse PCR, if the sequence at the end are unknown then how do we identify the desired restriction enzyme to cleave and form sticky ends prior to the formation of circular DNA?

[@SHUBHAMSINGH-gh6ln]

Same question

[@meghavatsa8191]

Very well explained 🙏🙏

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@radhaagarwal9407]

Hats off 🙏🙏🙏🙏 sir thanks for this video

[@bhagyashreechatterjee959]

Aamr akhon Mone hoi.. amr jibone zoology r kono chapter e r kono somossa hbena .. karon shomus biology ache ✌️✌️✌️✌️

[@shomusbiologyofficial]

I am glad to hear that you're getting benefit from my lectures. Stay tuned

[@ilmasiddiqui110]

make ur profile on instagram too sir ...so that more people get to know about you ...and get benefited by watching ur vdos..

[@shomusbiologyofficial]

Sure

[@tarannumkhatoon5070]

such me bahut hi achi vidio hai wo bhi hindi me

[@shomusbiologyofficial]

You're welcome

[@mukeshmurmu471]

Thank you sir achha hai

[@shomusbiologyofficial]

You're welcome

[@shreyatiwari5271]

Awsm sir ...thanku so much jo ap hm logo k liye itna struggle krte hai ...thanku sir ..God bless u Sir mujhe apse ek Favour chahiye plzz help us ... Sir microscopy ke kuch video ap jaldi krne ka try kr skte hai ... I m sure apki list me to hoge hi ye topic but me thoda jaldi krne ka abhi favour mag rhi hu ...i hope u accept my request..thanku sir

[@shomusbiologyofficial]

I will make many more videos in hindi

[@rishabhthakur3711]

Gjb sir

[@ImranAhmad-df3mi]

Thanks sir

[@shomusbiologyofficial]

You're welcome

[@ART-fs2wd]

Wow Sir your hindi 👌👌👌

[@shomusbiologyofficial]

You're welcome

[@nikitavatandar1666]

very nice vdo loved it

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@mamtarathore6641]

Very very nice 👏👏

[@shomusbiologyofficial]

You're welcome

[@pragyasingh6445]

Thanku very much sir for all videos

[@sweta8135]

Thanku sir.. It's really useful.

[@shomusbiologyofficial]

You're welcome

[@TathyabhandarbyAK]

Sir... Please make a video about how to help pcr for diagnosis disease

[@shomusbiologyofficial]

Okay sure

[@Chia5555]

Thnku so much sir

[@shomusbiologyofficial]

You're welcome

[@vasundhararaina6849]

Moreover in inverse PCR the DNA molecule used initially is basically wat a whole genomic DNA or a small part of it. Coz of its whole genome then adding a restriction enzyme may produce several small segments. How will we then isolate the desired one to be used for inverse PCR.

[@punambardhan9147]

I want to understand Degenerate PCR primer designing..Could you??

[@hemmahur1891]

Sir please make a video on Carrier detection in Haemophilia A PATIENTS by using linkage markers

[@biologyc554]

Nice video sir.

[@shomusbiologyofficial]

You're welcome

[@Prabhjotsingh-fs3vp]

Thanku sir make a video in Hindi medium ..... I wish u will make a video Hindi in future

[@shomusbiologyofficial]

Sure

[@yogeshanjansinger2872]

Thanku sir

[@shomusbiologyofficial]

You're welcome

[@shikhakumari6575]

Sir, kindly make a tutorial on lymphocyte trafficking.. I really need to understand it..

[@letstravel8840]

Plz presentation on blast and fasta

[@sunilkumarsahu2283]

Sambhu bro I love you

[@shomusbiologyofficial]

Thanks

[@indranisahu5527]

Nice sir ji 🤗🤗 PDF mil sakta hai Kya

[@arkhamyt6052]

Sir in nested PCR , then what is the need of normal primers? we can directly add the nested primers on the target sequence and amplify our desired gene. Then what is the need of normal primers then?

[@ASHWANI81576]

Please suggest any book of molecular biology i m bsc mlt student

[@Vanshikajain2]

If in the question paper given RT PCR then it is real time or reverse transcriptase PCR ???

[@ekramul3338]

Thank U sir........

[@BTS_army_V30]

Hello sir . semi-nested pcr pr b vedio bna de .iska concept clear nhi ho rha

[@prabhudasnair5636]

In Rt pcr why rna primer is used instead of dna primer? While in other type of pcr dna primer is used Please reply

[@zulqarnanh]

Awesome

[@shomusbiologyofficial]

You're welcome

[@Jubokay]

Flanking sequence we know

[@minakshee7240]

Nice sir

[@shomusbiologyofficial]

Thank you.

[@rubabikram4619]

if no plz tell the difference between nested or hemi-nested pcr

[@jyotisingh448]

Sir as we designed the second set of primers for the target dna in nested pcr why can't we designes the specific primers of the target dna for the common pcr.....sir kindly answer i really wanted to know

[@nathadil6765]

Sir, can you plz upload csir life science videos

[@shomusbiologyofficial]

Many videos are uploaded. There are more than 2300 videos

[@anilkohali6198]

thk u sir

[@shomusbiologyofficial]

You're welcome

[@davidsweeney111]

Shomu could you make this video in English as well please

[@shomusbiologyofficial]

It's already there

[@sunilkumarsahu2283]

Texonomy k upper video upload karo please

[@shomusbiologyofficial]

I will.

[@sahilprabhakar4788]

Sir plz give complete information about ICAR - ARS exam

[@gautambharali4492]

Late PCR kya hai please video sir

[@shomusbiologyofficial]

Okay

[@pk.educationclasses7733]

Nice video sir ekad uhi hindi m b video bana dijiyega

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@glowupwithnature145]

Why we don't use just nested primer ? Why we use 1st primer if it binds unwanted sequnces?

[@shomusbiologyofficial]

One primer set can make more unwanted amplification. Two set can reduce unwanted amplification

[@NehaRajput-ly7do]

Ur hindi is not that bad sir...... 🙂 ur method of teaching best in all the ways

[@shomusbiologyofficial]

Thank you

[@tanvisharma812]

Sir i have a question related to nested pcr.. That we are adding both outer and nested primers in a definite concentration... It is true that outer primer will amplify the unwanted DNA and nested one will amplify the target DNA.. But the whole process is going on for 30 cycles lets say... And the unwanted DNA along with outer primer they too are in the reaction till the reaction ends for 30 cycles how come we are going to identify wheather the unwanted DNA is not amplified as the reaction has outer primer and DNA till the end.... I mean are we adding any enzymes that will. Specifically cut the target DNA from the rest unwanted DNA

[@rishiivirgo]

by gel electrophoresis. best example of nested PCR is identification of Rotavirus

[@rajarora4592]

RNA RT-PCR is confermetory test and target not detected means

[@yashpathology]

Flanking sequence means sir

[@shomusbiologyofficial]

Means the sequence present as single stranded region of DNA

[@devarshkumar9955]

what type of polymerase we use in real time PCR as in taq man probe it uses exonuclease activity

[@shomusbiologyofficial]

Probe or dye

[@rubabikram4619]

is hemi-nested or semi-nested pcr same?

[@tarannumkhatoon5070]

👌👌👌☺

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@pinshusamariya7873]

Kya PCR hiv test me bhi kaam aata hai, or iski accuracy kitni he

[@rambhawan2643]

Evolution video in hindi please sir

[@Pearlblack6387]

i need notes of this video urgently. can anybody have?

[@ilatiwari2807]

Sir ,types of PCR ,csir net me pucha jata h kya?

[@shomusbiologyofficial]

Yes

[@hiteshpegu2844]

Can we use same pcr for testing dna of different species i.e human ,plant ,bacteria

[@shomusbiologyofficial]

Different chambers

[@juwairiahmazhar9607]

👍👍👍

[@jaidtechy2762]

Sir notes mil saktey hai?

[@kumaresangomathykumaresan7817]

Can you make videos in english plz

[@shomusbiologyofficial]

You will get this video in English in my channel as well

[@anmol786]

Sir aap BSC zoology honours ki whole video hindi me bnao either payed video ya free depend on your .....bt mghe need h kaise hoga pls help me sir..

[@sonamdhanava3098]

Will I eligible for UGC net exam after the M. Tech in cosmetics..?

[@villageofbotany3296]

Thanks Sir use Hindi language in your lectures

[@shomusbiologyofficial]

You're welcome

[@anuragsingh-hp4ko]

I think Ye topic eng m cover ho gya h?

[@shomusbiologyofficial]

Ues

[@vasundhararaina6849]

Nested always keeps me wondering ABT it's need. Wn one already has the inner primer set then y to use the external one. Anyway practically things are not so common wd the theoretical aspects.

[@rekhasaini7728]

Same here.... I am still not able to understand nested PCR 😢

[@ArslanAli-e8c]

Any one from physiology 2k23 batch 😂😂😅

[@shomusbiologyofficial]

All the best

[@shomusbiologyofficial]

All the best

[@kolasrinivasarao1096]

PCR English plz

[@shomusbiologyofficial]

Already there

[@Jubokay]

Kitna DNA amplify kr rha hai, we can also measure it.

[@shomusbiologyofficial]

In quantitative real time pcr