Introduction to plant circadian rhythms

[@wonsunparque4788]

Thank you for such a clear and easy-to-understand explanation of how plants' circadian rhythm works. And in such a short amount of time!

[@yudhistira7231]

You have my most sincere gratitude! Thank you🙏

[@divyanautiyal6302]

You are simply AWESOME.

[@Pinknightlover]

It helped me a lot to understand the whole topic, thank you very much

[@pritampritamarts236]

Awesome explanation maam....thanks for sharing this video with us ❤🙇‍♂️

[@marieramette953]

thank you for this video ! it's really usefull and interessant. Thank you !

[@shaynesemuzah567]

You are amazing!!

[@anoopkumarverma390]

Informative Lecture Ma'am...Thanks !!

[@alianatasha8577]

Thank you so much for your explanation! 💗

[@hafsakhan2294]

Sooper explanation

[@nelsonmachado5679]

Amazing explanation!! thanks!!!!!!

[@madman2547]

This is exactly what I was looking for, thank you so much. I have a question by the way: how do circadian and diurnal rhythm combine? I mean since the circadian rhythm is independent on environment and can't be changed, is it convenient to keep the standard 24h diurnal period or the plant could benefit from getting a different one? I kind of have the feeling some plants could grow more "assisted" with shorter diurnal periods

[@katharinehubbard5043]

Hi there - glad it was useful. So - in light-dark cycles there will be both the circadian rhythm and the acute response to light/dark contributing to the plant's physiology. The circadian clock is 'entrained' i.e. reset in response to light - the clock will be reset at dawn in light/cycles, whereas in continuous light each cycle might start a bit early/late depending on the period of the rhythm. Dodd et al 2015 show that growing plants with a 24 hour clock in anything other than a 24 hour light/dark cycle causes poor growth and survival - there is a selective advantage of having the genetic circadian oscillatory synchronised with the external environment. Hope that helps!

[@madman2547]

@@katharinehubbard5043 my feeling was wrong. Thanks for the reply! Sorry for any mistakes as I'm not a native english speaker

[@katharinehubbard5043]

@@madman2547 No problem - thanks for the question!

[@javierlozano8890]

But if gene A expression is dependent on light, doen't that mean that the sistem is not internal?

[@maxwellasiedu1876]

Hi What molecular technique would you employ to evaluate the difference in structure of the circadian clock in different plants? eg CC in wheat and arabidopsis

[@katharinehubbard5043]

Good question! There are lots of different ways you could do it. One approach would be a genomic one, identifying which of the Arabidopsis genes have homologues in wheat. That doesn't demonstrate the functionality of those genes though - you would need to do some reverse genetics to demonstrate that the absence of the gene affects clock function (which is very non-trivial in wheat). Alternatively you take the forward genetic approach and identify mutants in circadian rhythms or associated phenotypes like flowering time or hypocotyl elongation, and then do Genome Wide Association Studies to identify candidate genes. Can also do qPCR/RNAseq to look at the expression patterns of putative clock genes - nearly all clock components have a transcriptional rhythm, but so do downstream genes that are regulated by the clock - a rhythmic transcript by itself doesn't prove you are looking at a central oscillator gene. Hope that helps!

[@maxwellasiedu1876]

@@katharinehubbard5043 Very insightful! Thanks a lot Do you mind me asking further questions?

[@katharinehubbard5043]

@@maxwellasiedu1876 Ask away!

[@maxwellasiedu1876]

@@katharinehubbard5043 How do I quantify the contribution of a specific CC gene & it’s function to yield traits in the field?

[@annabeatrizkuffner6878]

you made it sound so easy and simple, I wish you could teach about every single one of my classes lol! thank you for the video and keep up with the good work!