Polyadenylation of mRNA (poly a tail)

[@bikkeychettri1333]

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you deserve more views ! maybe add tags so people can find you ! this is great !

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Wow.. you are a great teacher! Great way to explain this!! My graduate professor is extremely knowledgeable but doesn't know how to deliver the information. Thanks!! I understand now!!

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U. R. Brilliant. I. Mean. The. Way. U. R. Describing. All. Is. Very. Catchy. Its. Really. An. Easy. And. Effective. Way. To. Understand. Molecular. Biology. Ur. Videos. Helped. Me. Alot. Keep. It. Up. N. Keep. Helping. Us. The. Way. U. R. Doing. It. ★☆★☆

[@malikid1000]

You said the polyadenylation is added by RNA polymerase 2 which is wrong , it is added by another polymerase knows as PAP (Polyadenylation polymerase).

[@indrajeetsingh-ev3ig]

Yes in Aakash our teacher also told us it is by polyadynelate polymerase not RNAP 2 and clearly mentioned in class 12 biology chapter molecular basis of inheritance

[@mansikhandeparkar2764]

Thank you so much.... Your videos always help in getting very clear picture of the topic in a very easy way

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[@skmehabubalam6667]

Thanks you. Sir from your tutorial vidio i learned well and clear all doubt.and i had got topper in final year of B. Sc microbiology and now still i keep touching with your tutorial. Thank you so much

[@saadahmad9872]

Everything but the end was right. After CF1 and CF2 come in and cleave the pre-mRNA, the cleavage factors and CstF pop off and PAP (poly A polymerase) creates the poly A tail by elongating the 3' OH end. -Slow polyadenylation from 0-15 As -Fast polyadenylation up to 250 As

[@Tilloya]

Thank you for all your vids! These series about DNA transcription are super useful. It covers about 3 long hours of my bio mol cours! Beside, you explain very well and make all this process look easier :)

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Sir u r brilliant...you make it very simple to us to understand any topic thank you keep posting

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[@karabomoloisi1134]

ure amazing and the way you explain things its just so understandable and simplified. thank you very much for your video's

[@mamtaom6098]

Sir Bina book open kre teach krte h even diagram bi draw krte h clean.plz request to all..phele video full dekhe. Fir mistake btaye kbi kbi sir end me mistake' correct bi kr dete h. Shomu sir 👏👏👏

[@shomusbiologyofficial]

Thank you for your support

[@malikid1000]

The CSTF also binds to the GU region not the CA region.

[@tai_tai_fish]

what have been said by @abz down there is correct.. polyadylation is commenced by PAP (poly A polymerase) becasue rna pol 2 never come with m-RNA transcript .

[@ayeshashams3135]

Thank you sir your teaching skills are amazing

[@shomusbiologyofficial]

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[@pratibhamishra5370]

So simple way and much informative thank you

[@shomusbiologyofficial]

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[@tannerkim5644]

I think you might have some confusion, let me clarify few things. CSF what you mentioned is actually CstF and CstF binds to G/U rich region. Anyway, I like your lecture. Thanks for this lecture.

[@jesminsultana1700]

Thank you sir, your teaching skills are amazing ❤️

[@shomusbiologyofficial]

Thank you

[@akshatakale3167]

u r too good...helping me to get good marks ,,thanks a lot

[@preetisawarikar4564]

the diagram of this is present on pg 454 of weaver 5 th edition....awesome explanation by u too ...

[@souravdalapati.6270]

Cleavage stimulation factor(CST) binds on GU rich tract not on AAUAAA sequence along with CPSF.Please make it correct! Salute your work shomu da!

[@exploringworld5860]

instead of CA sequence it is GU sequence which is recognized by CSF.

[@sudipkumarmukhi726]

Such a hard work for all of us Tq 😀

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@kingmahi1]

Sir you're a king🙏

[@Banilicious89]

you were right , it is CstF for Cleavage stimulating Factor ... the st is for stimulation

[@joanne7699]

Hi Shomu could I ask does the 5' capping or the polyA tailing also help to prevent the miRNA from degrading the mRNA. Really appreciate if you could reply 😃

[@marymessam2435]

Thank you 💗

[@shomusbiologyofficial]

You're welcome

[@ekramhelmy1437]

May I ask why the tailing is specifically added by As repeats and not any other nucleotide (polyGs, polyTs, or PolyCs repeats)????

[@alebraheme]

i read article taking about some models that were designed for explain transcriptional regulation such as probabilistic model and quantitative model and other models but i can not understand them .. could u plze explain thus models for us

[@nifatjan760]

sir...why only G and A are added. 5'&3' respectively.why not pyramidine.

[@saktiranjanrout]

u r doing a nice job sir..thank you

[@shilpavenu9396]

Thank u soo much ...its really helpfull

[@shomusbiologyofficial]

You're welcome

[@mercuriallittlebitch]

thank you so much for your effort saving us = ) the genetic superhero!

[@sayeedachowdhury5598]

Excellent work!

[@namratadey6781]

sir cleavage factor cuts at specific site and PABP attaches at that position and so on....but what is the fate of the portion that relies after the cut site of CF...??Is it degraded?

[@yugandhar108]

Thank you so much suman.

[@Alexey_Heisenberg]

What is alternative polyadenylation

[@binduu19]

its really helpful...thank u sir..

[@Lovleen.kakkar]

sir please make video on topic "nuclear export of mRNA & RNA stability"

[@shomusbiologyofficial]

Thank You

[@sunainakaul6987]

can't endonuclease cleave the mrna??

[@aaqibjavid5623]

is it pol 2 which is adding Adenine or Poly A polymerase..... as pol 2 is template dependent

[@eragon2121]

Doesn't CstF recognize the GU sequence?

[@hafizmuhammadrehan074]

eragon2121 yes

[@sumanapal]

Is CstF not bind GU region?

[@ladwobhai]

Great work!! Thank you :)

[@Raptor9323]

i second rupert. but he helps.

[@mariamj9197]

The is really helpful..👍

[@jyotikumari-vc4ep]

I guess PAP performs polyadenylation not pol 2

[@faryalgohar8605]

it was very helpful :)

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Thank you!!!

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Thanks sir

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Keep it up!!!!

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thanks sir...

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Darun

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👍😊thank you

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Sir u r best in molecular bio... Pls reply or react on my comment....I will happy then

[@shomusbiologyofficial]

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Hindi ya to bengali use plese

[@samk4367]

lol the thumbnail looks like a meme

[@shomusbiologyofficial]

Ha ha

[@karthikparker8079]

As a student of biochemistry I kindly ask you to upload a theory part of genetic code , please sir.

[@shomusbiologyofficial]

Will make it soon.

[@indrajeetsingh-ev3ig]

Any neet UG aspirant here