Using a Micropipette - University of Leicester

A short film showing how to use a Gilson Micropipette. The film was made to support the teaching of undergraduate Biological Science students at the University of Leicester.
The film was produced by GENIE, based within the Genetics Department at the University of Leicester. Further information and materials can be found on the GENIE web site www.le.ac.uk/genie and the GENIE VGEC web site www.le.ac.uk/vgec
The film was directed by Jon Shears of ITS-Multimedia Services and the film was produced by Raymond Dalgleish, Nicola Suter-Giorgini and Cas Kramer.

[@cubehire3653]

This is the best Pipette instructional video I have found thank you.

[@ceciliarosenqvist9797]

Cube Hir. e

[@cmrnqueen]

only pipette video that shows how to read the the P2...Thank YOU!!!...just saved me from getting hell from my lab proff

[@DA-js7xz]

I laughed so hard at that sound effect for the "Don't Do's".

[@nicholaspark4165]

The Nos sounded like a broken tuba or a fart

[@burcus_ARTelier]

me too!! the sound...was great! :)

[@seven7wenty]

haha that's how you'll remember the do's and don'ts

[@valeriozippo5720]

poooo poooo X .......... LOL

[@Dinlek]

Without a doubt the most helpful and informative lab video I've watched. Thank you very much.

[@willdrew6825]

This video made me want to micropipette some liquids so bad

[@HZAexNB]

Not as easy as it looks mate

[@denwin4221]

No bro What my teacher could not explain Like first stop and second stop Easily explained by the mam ,for me to understand. Really helpful ❤️

[@melissarichten6323]

Same

[@Valgween]

Same

[@chriswong1516]

How a clear explanation. I'm going to do laboratory practical tomorrow. This may help a lot to me.

[@GrayWinters]

P2 range: 0.2-2ul P20 range: 2-20ul P200 range: 20-200ul P1000 range: 100-1000ul (in this case u is the symbol for micro, or 10^-6)

[@johannesmthembu8663]

Thanks University of Leicester for this video,well explained thank u.

[@christyxoxo]

lecturer told me to watch.. and i found it very useful!!! Thanks!!

[@KhanaHatake]

I had to watch this for uni, but it's actually so interesting!

[@korenmoscovich4681]

Literally the best pipetting video ever

[@dontownsend9724]

You've covered the possible ways the pipette can be contaminated or damaged,but you've failed to follow up on what to do about it. I started working in an analytical lab over 20 years ago and they were having trouble passing the unknown quantitative metals tests each year required by the EPA. My first year on these analysis's I was testing the output of my eppendorf pipette on an analytical balance with reagent grade water to insure in was delivering the proper volume before making my standards. Another analyst asked me what I was doing , I told them and they then said." well I worked in the medical field for over 10 years and we never tested our pipettes". All I could think was no wonder so many people die in hospitals if this is normal SOP in the medical field. If the pipette is found to be not delivering the set volume it must be taken apart and cleaned or repaired !!!! After this all our measuring equipment was checked closely for accuracy. We never failed our yearly test again .

[@TheSnakeboy11]

Awesome story, that's making the world a better place.

[@jauld360]

The first thing I did on receiving a new pipette was to test it. a) for accuracy, b) for repeatability and c) to check my technique. That analyst wasted 10 years.

[@bottledblue]

thanks heaps - helped my prepare for my first molecular biology lab! :)

[@xeryd6037]

same but 6 years later

[@nafis_rk]

@@xeryd6037 same but a month later

[@gilong1311]

Awesome video, answered all my questions.

[@austindiehl6540]

This video was terrific. Thank you

[@Hiphop101ize]

Oh yeah, all this talk about the tip got my body tingly!

[@KeptNow]

not bad for an instructional video. these science instructions are usually 20 years old and out of date. this video was really well done--with camera angles, explanation, and reasonings

[@BioLogicalNerd]

Thank you for this, I'm a micropipette pro now!

[@anjichant543]

That was an excellent revision of my 4 years of study in lab.

[@JoChen-gb4xr]

honestly, the part which the video demonstrates dos and don'ts is the best

[@김규형]

정말 유익해요! 과학 실험을 진행하는데 필요한 기본적인 지식들이 알차게 들어있네요!!

[@elenaautumnalmermaid6913]

As a biochemistry student, my heart breaks everytime the liquids touch the actual pipette. But great video for learning what to do and what not to do.

[@deadbzeus]

I feel this lol

[@oreadeboye4506]

Thank you so much for the video. You made pipetting so easy to understand.

[@KingofDundee]

Sounds like Douglas from the IT Crowd, great video!

[@cherylpatel8197]

Such a helpful video! Very thorough.

[@fireemblem2770]

I agree!!

[@razahasanbhatti8454]

Thank you very much very informative video. kindly tell me how to pack culture of nitrifying bacteria in sashay or a bottle actually i am asking about the media some people pack bacteria in powder form which media they use to bring alive bacteria into powder form?

[@stagbio4396]

Hello- I have used this video for instructional purposes for college freshman biology lab for years - THANK YOU for the thorough explanations. I see that Hungarian subtitles were added, and I am wondering if there a version with English subtitles still exists? Thank you

[@ifrahjameel4588]

best explanation so far

[@cherryy_2468]

this was honestly a really helpful video thank you

[@noshinnamira6608]

this is so detailed, i learned a lot from it

[@nayabghazali5455]

thats the best video i hv found so far!

[@Teo_live]

Great video. The [X] don't-do's were entertaining

[@KulbirAhluwalia]

This video is so good 😍😍😍 Covered the smallest details Awesome work

[@brookebain7250]

Thanks for this video! My professor didn’t go over how to set the volumes and I was so lost

[@Nayyarscorner93]

greatest demonstration ive evr seen good job

[@spkspk1416]

Literally I found gem in the form of this video

[@a.s9509]

If we have to use the second stop to eject the volume into the receiver tube there is going to be bubbles with that which is going to give you inaccurate results when say you are measuring the OD of serum samples. And if we don't use the second stop there would still be liquid in the pipet tips. What do we have to do?

[@jtxc3]

Thank you! This is brilliant! It helped me review for my lab mid-term

[@rabindrakhatiwada3558]

is there genetic engineering lecture series? if yes than please do post it.

[@kirad1947]

I have a Biology lab pre-quiz tomorrow, haha thank you

[@CWGminer]

Same

[@marcelomesa2154]

Excellent video, thank you for bringing such a great content, University of Leicester! Subscribed : )

[@mohammedahmedjalloh531]

This video is super helpful... so very detailed

[@quack703]

This video truly helped me! Thank you!

[@Sp-fe1eo]

thank you so much for the video...it's really cool and easy to understand

[@joanne7699]

Hi may I ask does the Pipette need to be vertical at all times even when dispensing liquid and Does the pipette tip need to be against the wall of the receiving tube during dispensing ? Thank you

[@bhaktisatam726]

Thanks for risking your pipette for us

[@ifeyinwaobikwelu1314]

A Very Good video, detailed and well explained. this video really helps. thank you

[@selamyemane1640]

You have been very helpful. Thanks

[@raghadalani9706]

Nice work.. I understand now... Thank you very much 👍👍👌😊😊

[@fabyalauture5929]

Thank you this video explain the process of pipetting very clearly

[@ayeshashaikh9729]

Thanks a lot for the best video 😊💗

[@lglintott]

Brilliant. Putting this on my teaching lab page.

[@sobster123]

Best pippetting vid out there!

[@Evemaru2]

Best video tutorial I have seen.

[@fyre1]

very good video, 10/10

[@lizziesinclair4254]

Thank you so much for making this video!

[@chauhuynh8158]

Wonderful video, thank you for helping me with my lab

[@lalayatem3758]

amazing video , thank you and God bless!

[@zahidwajdan2672]

Best Explanation, and of course Sound Effect.

[@hamismwinyi3723]

Thank you, this video is really helpful

[@akritino7437]

Thank you for such an informative video ! Loved it :)

[@liying1994]

Excellent video.

[@minasdrakos7544]

excellent, very analytic!

[@fatimainigo25]

Best Video! Thank you very much!

[@lucysnowe9974]

Very useful tips, thank you. :)

[@hazelezeribe5767]

Soooo Helpful!! Thank you: :)

[@mikejedi182]

Thank you for creating this video

[@lyubomirgizdarski8191]

Great video!

[@yeyhu4767]

excellent video, thank you !

[@palashmassey]

wonderful...love it and learn it..keep uploading please

[@yolniejacques8117]

This video is very helpful, thanks!

[@victorenriqueriosortega5917]

Es un video muy interesante sobre el uso de las micropipetas, desconocía qué algunas pipetas tenían en el botón un número lo que nos dice hasta cuanto puede medir, además de los números rojos qué también sirven para lo mismo

[@andraskovacs5247]

fantastic video

[@natxnatxnat]

What a brilliantly useful and helpful video. Thank you!

[@Dankstv]

thank you for this info that ill prolly never use lol im not even a biologist or chemist

[@timecode37]

I've been told to repeatedly wet the inside of the pipette tip by carefully pipetting up and down to the first stop 2-3 times with the liquid to get the accurate volume, is this not necessary?

[@sonicandroxas]

Hey did you figure if this is true or not?

[@timecode37]

@@sonicandroxas I've heard it in other videos from different manufacturers, so it might be true

[@FieldGamers]

This was perfect. Thank you

[@estelamestellaestellaiscry4417]

Excellent PLEASE .give examples of calculation of valume...

[@rustyjeanz]

Nice explanation. However I am confused at 2:44 so if I have to 152 uL using P1000, then what should be the number on pipette?

[@timecode37]

You couldn't set the P1000 to 152 µL, it would only let you set the µL in sets of five, so you could set 150 and 155, 160 and so on.

[@sofiabergstrom41]

Do they pipette in micro-gallons and milli-cups in America?

[@riyadhdahham2075]

Cood... amazing explains Thank you شكرا

[@jadem.7227]

very helpful! way better than the shitty video my school gave me!!

[@MrJason005]

Guess I should have applied to study at Leicester. Sheffield doesn't have as high quality teaching material as this video

[@nethuakashi432]

All in one.... Thanks so much ❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️

[@kkmatt]

¡Muy excellente! Totally ready for lab!

[@microfetish]

A lovely job indeed!

[@Khemani_RL]

I love these sound effects 😂

[@gabvela8192]

Excellent explanation thank you!

[@Navid115]

awesome video

[@RoyAndrews82]

***** I have a question. What about working with thick, viscous liquids? Can a micropipette work with thick, viscous liquids such as vegetable glycerin? I need to work with vegetable glycerin in amounts of 100uL. thanks for the video, very helpful (+1 : 430)

[@VJamrita]

Can use a positive pressure pipette

[@bblahbulah]

Thanks, so helpful

[@sharon_ng]

+UniversityLeicester How should we clean the cross-contaminated pipettes then?

[@LoveResidentEvil4]

Thank you :) helps my lab bio a lot :)

[@sasa012]

great video ...

[@thanhmiennguyen895]

Thank you so much. It's useful.

[@Hackmeister0]

University of Sydney Baby !

[@thewirelessbrain9331]

perfect explanation, thank you!

[@francescorusso1729]

I always take low volumes or can't take any volume. What is the problem?

[@MrFluffster101]

Is... is that Matt Berry narrating this video?

[@sanghmitrasingh4245]

thanks sir please explain me about digest dna what is that???